基于电解槽流动场的分析,利用ANSYS软件模拟计算了SY500铝电解槽的流动场形态分布及界面变形,同时对某铝厂500 k A电解槽进行流动场测试,并将铝液流动方向和流动速度的模拟计算结果与实测结果进行对比分析,对比结果存在一定的偏差,但整...基于电解槽流动场的分析,利用ANSYS软件模拟计算了SY500铝电解槽的流动场形态分布及界面变形,同时对某铝厂500 k A电解槽进行流动场测试,并将铝液流动方向和流动速度的模拟计算结果与实测结果进行对比分析,对比结果存在一定的偏差,但整体是相互吻合的,即基于数值模拟的电解槽流动场方法是准确可靠的。展开更多
5Ni/（10NiO-NiFe2O4） cermet materials were prepared by cold pressing sintering method in different atmospheres. The phase compositions were studied by XRD, microstructure was studied by SEM and mechanical properties ...5Ni/（10NiO-NiFe2O4） cermet materials were prepared by cold pressing sintering method in different atmospheres. The phase compositions were studied by XRD, microstructure was studied by SEM and mechanical properties were studied by three-point-bending strength and heat cycle. The results show that Ni/（NiO-NiFe2O4） cermet can be obtained by sintering in vacuum or atmospheres with oxygen content of 2×10-5, 2×10-4 and 2×10-3, respectively, but the phase composition of the ceramics varies greatly with oxygen content of the sintering atmosphere. The cermet tends to have a high content of NiO when sintered in vacuum. The content of NiFe2O4 and Ni in the cermet materials respectively increases and decreases with increasing oxygen in the sintering atmosphere. In the vacuum, cermet has an average grain size of 3.90 μm and bending strength of 138.59 MPa, which is able to withstand thermal shock tests 6.67 times in average.展开更多
Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples o...Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers（6 subjects in each of the 3 groups） were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups： H0（no HA）, H100（HA, 0.1 g/L） and H500（HA, 0.5 g/L）. The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results：HA promoted proliferation of chondrocytes in all the three groups, and.in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 〈 HA100 〈 H0. Conclusion：Sodium hyaluronate administration has a dosedependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.展开更多
This study aimed to investigate the effect of hyaluronic acid(HA)on the expression of heat-shock protein 70(HSP70)in chondrocytes isolated from patients with osteoarthritis(OA)and Kashin-Beck disease(KBD).The chondroc...This study aimed to investigate the effect of hyaluronic acid(HA)on the expression of heat-shock protein 70(HSP70)in chondrocytes isolated from patients with osteoarthritis(OA)and Kashin-Beck disease(KBD).The chondrocytes were collected from OA and KBD patients,and chondrocytes isolated from patients of accident injuries were used as the control.The chondrocytes were treated with HA at different doses.HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis.Compared with control,both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA.However,HA at the dose of 500μg/mL significantly inhibited HSP70 expression levels in both KBD and OA groups(P<0.05).In conclusion,HSP70 is highly expressed in chondrocytes of patients of OA and KBD.HA intervention inhibits the upregulation of HSP70 in chondrocytes of OA and KBD patients and could be a promising agent for treatment of OA and KBD.展开更多
Nutritional diarrhea and subsequent performance degradation in weaned piglets are major challenges for the pig industry.Bile acids(BA)can be added to the diet as emulsifiers.This experiment was conducted to investigat...Nutritional diarrhea and subsequent performance degradation in weaned piglets are major challenges for the pig industry.Bile acids(BA)can be added to the diet as emulsifiers.This experiment was conducted to investigate the effects of chenodeoxycholic acid(CDCA),a major primary BA,on growth performance,serum metabolic profiles and gut health in weaned piglets.A total of 72 healthy weaned piglets were randomly assigned to the control(CON)and the CDCA groups,which were feed a basal diet and the basal diet supplemented with 200 mg/kg CDCA for 30 d,respectively.Our results demonstrated that CDCA significantly increased final BW and average daily gain(ADG),decreased feed-to-gain(F:G)ratio and tended to reduce diarrhea incidence.In addition,CDCA increased the villus height-to-crypt depth(V:C)ratio,elevated goblet cell numbers and the expression of tight junction proteins,suggesting the enhancement of intestinal barrier function.As an emulsifier,CDCA increased jejunal lipase activity and the mRNA expression of pancreatic lipases.CDCA supplementation also altered the serum metabolic profiles,including increasing the levels of indole 3-acetic acid,N'-formylkynurenine and theobromine that were beneficial for gut health.Moreover,the relative abundance of 2 beneficial gut bacteria,Pre-votella 9 and Prevotellaceae TCG-001,were increased,whereas the relative abundance of a harmful bacteria,Dorea,was decreased in the gut of weaned piglets supplemented with CDCA.Importantly,the altered serum metabolic profiles showed a strong correlation with the changed gut bacteria.In conclusion,CDCA improved the growth performance of weaned piglets by improving intestinal morphology and barrier function,and enhancing lipid digestion,accompanied by alterations of serum metabolic profiles,and changes in relative abundance of certain gut bacteria.展开更多
Dear Editor,Kidney stones are one of the most common clinical diseases in urology.Approximately 80%of renal calculi are composed of calcium oxalate(CaOx)crystals,which are gradually deposited in the medullary collecti...Dear Editor,Kidney stones are one of the most common clinical diseases in urology.Approximately 80%of renal calculi are composed of calcium oxalate(CaOx)crystals,which are gradually deposited in the medullary collecting duct or in the renal interstitium,causing nephrocalcinosis.1 Although CaOx nephrocalcinosis is usually asymptomatic.展开更多
文摘基于电解槽流动场的分析,利用ANSYS软件模拟计算了SY500铝电解槽的流动场形态分布及界面变形,同时对某铝厂500 k A电解槽进行流动场测试,并将铝液流动方向和流动速度的模拟计算结果与实测结果进行对比分析,对比结果存在一定的偏差,但整体是相互吻合的,即基于数值模拟的电解槽流动场方法是准确可靠的。
基金Project (2001AA335013) supported by the National High-tech Research and Development Program of China
文摘5Ni/（10NiO-NiFe2O4） cermet materials were prepared by cold pressing sintering method in different atmospheres. The phase compositions were studied by XRD, microstructure was studied by SEM and mechanical properties were studied by three-point-bending strength and heat cycle. The results show that Ni/（NiO-NiFe2O4） cermet can be obtained by sintering in vacuum or atmospheres with oxygen content of 2×10-5, 2×10-4 and 2×10-3, respectively, but the phase composition of the ceramics varies greatly with oxygen content of the sintering atmosphere. The cermet tends to have a high content of NiO when sintered in vacuum. The content of NiFe2O4 and Ni in the cermet materials respectively increases and decreases with increasing oxygen in the sintering atmosphere. In the vacuum, cermet has an average grain size of 3.90 μm and bending strength of 138.59 MPa, which is able to withstand thermal shock tests 6.67 times in average.
基金supported by the Ministry of Science and Technology(2006 DFA33610)the Natural Scientific Fund of China(30630058)the International Co-operative Fund in Shaanxi(2005KW-13)
文摘Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers（6 subjects in each of the 3 groups） were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups： H0（no HA）, H100（HA, 0.1 g/L） and H500（HA, 0.5 g/L）. The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results：HA promoted proliferation of chondrocytes in all the three groups, and.in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 〈 HA100 〈 H0. Conclusion：Sodium hyaluronate administration has a dosedependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.
基金Supported by Project of Science and Technology of Social Development in Shaanxi Province(No.2013SF2-10)Shaanxi Science&Technology Coordination&Innovation Project(No.2015KTCQ03-01).
文摘This study aimed to investigate the effect of hyaluronic acid(HA)on the expression of heat-shock protein 70(HSP70)in chondrocytes isolated from patients with osteoarthritis(OA)and Kashin-Beck disease(KBD).The chondrocytes were collected from OA and KBD patients,and chondrocytes isolated from patients of accident injuries were used as the control.The chondrocytes were treated with HA at different doses.HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis.Compared with control,both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA.However,HA at the dose of 500μg/mL significantly inhibited HSP70 expression levels in both KBD and OA groups(P<0.05).In conclusion,HSP70 is highly expressed in chondrocytes of patients of OA and KBD.HA intervention inhibits the upregulation of HSP70 in chondrocytes of OA and KBD patients and could be a promising agent for treatment of OA and KBD.
基金the National Natural Science Foundation of China(31672508,31972636,31790411)the National Key Research and Development Program of China(2017YFD0500501)+2 种基金South China Agricultural University Doctoral Innovative Talent Cultivation Program(CX2019N006)Guangdong Key areas Research and Development Project(2019B020218001)Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry.
文摘Nutritional diarrhea and subsequent performance degradation in weaned piglets are major challenges for the pig industry.Bile acids(BA)can be added to the diet as emulsifiers.This experiment was conducted to investigate the effects of chenodeoxycholic acid(CDCA),a major primary BA,on growth performance,serum metabolic profiles and gut health in weaned piglets.A total of 72 healthy weaned piglets were randomly assigned to the control(CON)and the CDCA groups,which were feed a basal diet and the basal diet supplemented with 200 mg/kg CDCA for 30 d,respectively.Our results demonstrated that CDCA significantly increased final BW and average daily gain(ADG),decreased feed-to-gain(F:G)ratio and tended to reduce diarrhea incidence.In addition,CDCA increased the villus height-to-crypt depth(V:C)ratio,elevated goblet cell numbers and the expression of tight junction proteins,suggesting the enhancement of intestinal barrier function.As an emulsifier,CDCA increased jejunal lipase activity and the mRNA expression of pancreatic lipases.CDCA supplementation also altered the serum metabolic profiles,including increasing the levels of indole 3-acetic acid,N'-formylkynurenine and theobromine that were beneficial for gut health.Moreover,the relative abundance of 2 beneficial gut bacteria,Pre-votella 9 and Prevotellaceae TCG-001,were increased,whereas the relative abundance of a harmful bacteria,Dorea,was decreased in the gut of weaned piglets supplemented with CDCA.Importantly,the altered serum metabolic profiles showed a strong correlation with the changed gut bacteria.In conclusion,CDCA improved the growth performance of weaned piglets by improving intestinal morphology and barrier function,and enhancing lipid digestion,accompanied by alterations of serum metabolic profiles,and changes in relative abundance of certain gut bacteria.
基金This work was supported by the National Natural Science Foundation of China(82070726,82060137,81970604,and 82100808)the Key Project of the Guangxi Natural Science Foundation(2020GXNSFDA297013)+2 种基金the China Postdoctoral Science Foundation(2021M690185)the Anhui Postdoctoral Science Foundation(2021A482)the Anhui Provincial Department of Education Research Project(KJ2020A0167).
文摘Dear Editor,Kidney stones are one of the most common clinical diseases in urology.Approximately 80%of renal calculi are composed of calcium oxalate(CaOx)crystals,which are gradually deposited in the medullary collecting duct or in the renal interstitium,causing nephrocalcinosis.1 Although CaOx nephrocalcinosis is usually asymptomatic.
