该研究以黄瓜“津春2号”cDNA为模板,采用RT-PCR方法克隆得到黄瓜叶绿素降解关键酶(pheophorbide a oxygenase,PAO)基因(CsPAO),对其进行亚细胞定位观察,并采用实时荧光定量PCR技术和生物信息学技术,分析了CsPAO基因的表达模式及其编码...该研究以黄瓜“津春2号”cDNA为模板,采用RT-PCR方法克隆得到黄瓜叶绿素降解关键酶(pheophorbide a oxygenase,PAO)基因(CsPAO),对其进行亚细胞定位观察,并采用实时荧光定量PCR技术和生物信息学技术,分析了CsPAO基因的表达模式及其编码蛋白的特性。结果表明:(1)CsPAO编码545个氨基酸,理论等电点为6.09,蛋白相对分子质量为61.02 kD。蛋白预测发现,黄瓜CsPAO属于不稳定蛋白,具有2个蛋白结合位点,且存在跨膜现象。(2)荧光定量PCR结果表明,CsPAO基因表达响应水杨酸(SA)、茉莉酸(JA)和赤霉素(GA3)的调控,在高温(42℃)和低温(4℃)处理下CsPAO基因的表达量显著上升并达到最高,但黑暗处理对CsPAO基因表达没有影响;在黄瓜不同组织中花的表达显著高于根、茎、叶、萼、须、果。(3)亚细胞定位结果表明,CsPAO蛋白定位于叶绿体内。(4)系统进化树分析显示,黄瓜CsPAO与葫芦科植物苦瓜、西葫芦、南瓜、笋瓜等亲缘关系较近。本研究结果为进一步揭示黄瓜叶绿素降解的分子机制奠定了基础。展开更多
PhaseⅠcommissioning of the SSRF storage ring at 3.0 GeV was ended with encouraging results. Distortions and calibrations of the linear optics during the storage ring commissioning are discussed in this paper.The cali...PhaseⅠcommissioning of the SSRF storage ring at 3.0 GeV was ended with encouraging results. Distortions and calibrations of the linear optics during the storage ring commissioning are discussed in this paper.The calibration procedure has reduced sextupole-additional focusing effects by minimizing closed orbit deviation and corrected quadrupole magnetic coefficients with the linear optics from closed orbit (LOCO) technique.After fitting the closed orbit response matrix,linear optics of the storage ring is substantially corrected,and the measured parameters agree well with the design.Four optics modes were commissioned,and relevant machine physics studies were carried out.Their results are summarized.展开更多
The Shanghai Synchrotron Radiation Facility (SSRF) is a low emittance third-generation synchrotron radiation light source under commissioning. The excitation curve calibration for the booster magnets is important to p...The Shanghai Synchrotron Radiation Facility (SSRF) is a low emittance third-generation synchrotron radiation light source under commissioning. The excitation curve calibration for the booster magnets is important to provide the magnet current configurations as reference. Calibration studies give the polynomial coefficients of each type of magnets and provide the magnet current configurations under different beam energies as beam is ramped at speed of 2 Hz. The applications of calibration in booster commissioning which show the accuracy of the magnetic excitation curve calibration are also discussed.展开更多
In this paper,we report a MATLAB-based GUI tool,bodgui,which integrates functions of lattice editor,linear match,and nonlinear optimization,and visualized tracking functions for beam optics design.A user can switch hi...In this paper,we report a MATLAB-based GUI tool,bodgui,which integrates functions of lattice editor,linear match,and nonlinear optimization,and visualized tracking functions for beam optics design.A user can switch his/her design procedures one to another.Flexibilities are provided for adjusting or optimizing the lattice settings in commissioning or operation of the accelerators.The algorithm of the linear match and nonlinear optimization,and the GUI windows including the main functions and running status,are presented.The SSRF storage ring was employed as a test lattice.Several optics modes designed and optimized by the GUI tools were used for commissioning the storage ring.Functions of bodgui tool are machine-independent,and it can be well applied to modern light sources being built in other parts of the world.展开更多
文摘该研究以黄瓜“津春2号”cDNA为模板,采用RT-PCR方法克隆得到黄瓜叶绿素降解关键酶(pheophorbide a oxygenase,PAO)基因(CsPAO),对其进行亚细胞定位观察,并采用实时荧光定量PCR技术和生物信息学技术,分析了CsPAO基因的表达模式及其编码蛋白的特性。结果表明:(1)CsPAO编码545个氨基酸,理论等电点为6.09,蛋白相对分子质量为61.02 kD。蛋白预测发现,黄瓜CsPAO属于不稳定蛋白,具有2个蛋白结合位点,且存在跨膜现象。(2)荧光定量PCR结果表明,CsPAO基因表达响应水杨酸(SA)、茉莉酸(JA)和赤霉素(GA3)的调控,在高温(42℃)和低温(4℃)处理下CsPAO基因的表达量显著上升并达到最高,但黑暗处理对CsPAO基因表达没有影响;在黄瓜不同组织中花的表达显著高于根、茎、叶、萼、须、果。(3)亚细胞定位结果表明,CsPAO蛋白定位于叶绿体内。(4)系统进化树分析显示,黄瓜CsPAO与葫芦科植物苦瓜、西葫芦、南瓜、笋瓜等亲缘关系较近。本研究结果为进一步揭示黄瓜叶绿素降解的分子机制奠定了基础。
文摘PhaseⅠcommissioning of the SSRF storage ring at 3.0 GeV was ended with encouraging results. Distortions and calibrations of the linear optics during the storage ring commissioning are discussed in this paper.The calibration procedure has reduced sextupole-additional focusing effects by minimizing closed orbit deviation and corrected quadrupole magnetic coefficients with the linear optics from closed orbit (LOCO) technique.After fitting the closed orbit response matrix,linear optics of the storage ring is substantially corrected,and the measured parameters agree well with the design.Four optics modes were commissioned,and relevant machine physics studies were carried out.Their results are summarized.
文摘The Shanghai Synchrotron Radiation Facility (SSRF) is a low emittance third-generation synchrotron radiation light source under commissioning. The excitation curve calibration for the booster magnets is important to provide the magnet current configurations as reference. Calibration studies give the polynomial coefficients of each type of magnets and provide the magnet current configurations under different beam energies as beam is ramped at speed of 2 Hz. The applications of calibration in booster commissioning which show the accuracy of the magnetic excitation curve calibration are also discussed.
文摘In this paper,we report a MATLAB-based GUI tool,bodgui,which integrates functions of lattice editor,linear match,and nonlinear optimization,and visualized tracking functions for beam optics design.A user can switch his/her design procedures one to another.Flexibilities are provided for adjusting or optimizing the lattice settings in commissioning or operation of the accelerators.The algorithm of the linear match and nonlinear optimization,and the GUI windows including the main functions and running status,are presented.The SSRF storage ring was employed as a test lattice.Several optics modes designed and optimized by the GUI tools were used for commissioning the storage ring.Functions of bodgui tool are machine-independent,and it can be well applied to modern light sources being built in other parts of the world.
