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家蝇抗菌肽MDC协同庆大霉素抗普通变形杆菌作用研究 被引量:4
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作者 陈晴汝 桂水清 卢雪梅 《生物技术通报》 CAS CSCD 北大核心 2020年第11期85-93,共9页
以普通变形杆菌(Bacillus proteus vulgaris)为研究对象,探讨家蝇抗菌肽(Musca domestical cecropin,MDC)和庆大霉素(Gentamicin)的体外联合抗菌效果及协同抗菌机制。采用微量肉汤稀释法、棋盘法、结晶紫染色等方法研究两药的协同抗菌... 以普通变形杆菌(Bacillus proteus vulgaris)为研究对象,探讨家蝇抗菌肽(Musca domestical cecropin,MDC)和庆大霉素(Gentamicin)的体外联合抗菌效果及协同抗菌机制。采用微量肉汤稀释法、棋盘法、结晶紫染色等方法研究两药的协同抗菌作用和抗生物膜作用;通过荧光染色、流式细胞术(FCM)、透射电子显微镜(TEM)观察两药联用的抗菌机制。家蝇抗菌肽MDC与庆大霉素的FIC为0.313,表明两者具有较好的协同抗菌作用;结晶紫染色结果显示两药对细菌初始生物膜、成熟生物膜和胞外蛋白质的影响也有一定的协同作用;荧光和流式细胞术结果显示药物可以有效杀伤细菌,使细菌死亡数目上升;透射电镜观察到给药后大部分细菌细胞壁破损,细胞膜不完整,胞质内容物坏死溶解导致细胞肿胀破裂。家蝇抗菌肽MDC和庆大霉素具有协同抗普通变形杆菌作用,且两药的作用机制相辅相成。 展开更多
关键词 普通变形杆菌 家蝇抗菌肽 庆大霉素 协同抗菌
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Grinding process within vertical roller mills: experiment and simulation 被引量:11
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作者 WANG Jian-huai chen qing-ru +2 位作者 KUANG Ya-li LYNCH A j ZHUO Jin-wu 《Mining Science and Technology》 EI CAS 2009年第1期97-101,共5页
Based on screening analysis, laser size analysis, grindability and rigidity tests of samples collected on line from a cement and a power plant, a simulation of the grinding process in vertical roller mills was carried... Based on screening analysis, laser size analysis, grindability and rigidity tests of samples collected on line from a cement and a power plant, a simulation of the grinding process in vertical roller mills was carried out. The simulation calculation used a breakage function, B. The results indicate that the breakage function, B, and the selection function, S, in the form of a matrix, can be used to express the probability of the material breaking during the grinding process. This allows the size distribution of the product to be numerically estimated. The simulation results also show that the simulated size distribution curves fit the actual experimental product curves quite well. The model provides a good starting point for simulation of the grinding process. Further research is needed to determine the proper breakage function and the matrix value of the selection function. 展开更多
关键词 vertical roller mill GRINDING SIMULATION
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Non-isothermal Kinetics of Pyrolysis of Three Kinds of Fresh Biomass 被引量:10
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作者 MIN Fan-fei ZHANG Ming-xu chen qing-ru 《Journal of China University of Mining and Technology》 EI 2007年第1期105-111,共7页
The pyrolysis kinetics of three different kinds of fresh biomass (grass: triple A, wheat straw, corn straw) in nitrogen flow were studied by thermogravimetric analysis at five different heating rates. The kinetic para... The pyrolysis kinetics of three different kinds of fresh biomass (grass: triple A, wheat straw, corn straw) in nitrogen flow were studied by thermogravimetric analysis at five different heating rates. The kinetic parameters of the pyrolysis process were calculated using the method of Ozawa-Flynn-Wall and the mechanism of reactions were investi- gated using the method of Popescu. It was found that the values of activation energy varied in different temperature ranges. The pyrolysis processes are well described by the models of Zhuravlev (Zh) and valid for diffusion-controlled between 200 ℃ and 280 ℃, by Ginstling-Brounshtein (G-B), valid for diffusion-control between 280 ℃ and 310 ℃, for first-order chemical reaction between 310℃ and 350 ℃, by Zhuravlev (Zh) valid for diffusion-control between 350 ℃ and 430 ℃ and by the one-way transport model when temperatures are over 430 ℃. 展开更多
关键词 non-isothermal kinetics fresh biomass PYROLYSIS thermogravimetric analysis
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Study on the Reaction Mechanism of Naphthalene with Oxalyl Chloride 被引量:3
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作者 Wu Lin Qin Yi-min +4 位作者 Huang Bo Zong Zhi-ming Wei Xian-yong chen qing-ru Zou Guo-lin 《Wuhan University Journal of Natural Sciences》 EI CAS 2001年第4期854-858,共5页
The reaction of naphthalene with oxalyl chloride in the presence of anhydrous AlCl3 was investigated. The homolog of dinaphthyl methanone can be obtained mainly from this reaction. Naphthalene conversion does not have... The reaction of naphthalene with oxalyl chloride in the presence of anhydrous AlCl3 was investigated. The homolog of dinaphthyl methanone can be obtained mainly from this reaction. Naphthalene conversion does not have evident correlation with the amount of AlCl3. The results show that the reaction proceeds via carbon cation electrophilic substitution reaction-free radical substitution reaction pathway. 展开更多
关键词 NAPHTHALENE oxalyl chloride carboncation free radical reaction mechanism
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Effect of Pre-oxidation on the Properties of Crushed Bituminous Coal and Activated Carbon Prepared Therefrom 被引量:2
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作者 WANG Qi-bao ZHANG Xiang-lan +1 位作者 XU De-ping chen qing-ru 《Journal of China University of Mining and Technology》 EI 2007年第4期494-497,共4页
The influence of a pre-oxidation process on the chemical properties of crushed bituminous coal and on adsorption properties of the subsequently formed char and activated carbon is discussed in this paper. Datong bitum... The influence of a pre-oxidation process on the chemical properties of crushed bituminous coal and on adsorption properties of the subsequently formed char and activated carbon is discussed in this paper. Datong bituminous coal samples sized 6 mm were oxidized at different temperatures and for different times and then carbonized and activated by steam to obtain activated carbons. A Uniform Design method was used to arrange the experiments,IR and adsorption experiments were used to characterize these oxidized coals,chars and activated carbon samples. The results show that the carboxyl group disappeared and α-CH2 groups joined to alkenes decreased dramatically but the carbonyl group clearly increased in the coal sample oxidized at 543 K; The chemical composition of coal samples oxidized at lower temperature is different from that of coal oxidized at 543 K. Oxidizing coal samples at higher temperatures for a short time or at lower temperatures for a longer time resulted in activated carbon samples that tended toward the same adsorption properties: Iodine number 1100 mg/g and Methylene blue value 252 mg/g. The yield of activated carbon obtained from the pre-oxidized coal is 10% higher than the yield from parent coal but the activated carbons have the same adsorption properties. 展开更多
关键词 bituminous coal OXIDATION activated carbon chemical structure
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Combined Removal of Surface Moisture and Dust from Feed Coal for Coal Dry Cleaning with an Air-solid Fluidized Bed
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作者 YANG Guo-hua ZHAO Yue-min chen qing-ru 《Journal of China University of Mining and Technology》 EI 2005年第4期283-287,共5页
A demonstration plant and a commercial plant employing coal dry cleaning technology with an air-solid fluidized bed were built in China. The operation practice of these two plants shows that the surface moisture and t... A demonstration plant and a commercial plant employing coal dry cleaning technology with an air-solid fluidized bed were built in China. The operation practice of these two plants shows that the surface moisture and the fines or dust of feed coal must be well controlled as low as possible. For this purpose, a new process of combined removal of surface moisture and dust from feed coal using a vibrated fluidized bed dryer was investigated in a batch test apparatus and a pilot test system. A mathematical model on drying kinetics of coal surface moisture was developed and three empirical formulas of the model coefficient involving the main operating variables were determined based on the test results from the batch test apparatus. The mathematical model shows that the surface moisture retained in coal during drying decreases exponentially with drying time. According to this model, a new divisional heat supply mode, in which the inlet gas of higher temperature was introduced into the fore part of the dryer and the inlet gas of lower temperature into the rear part of the dryer, was employed in the pilot test system. The pilot tests show that 1) the new divisional heat supply mode is effective for lowering down the average temperature and reducing the total heat loss of the outlet gas off the dryer, 2) the moist coal of about 60 g/kg surface moisture contentcan be dried to about 10 g/kg, and simultaneously the fines (〈1mm in diameter) adhering to the surface of coarse coal particles are completely washed off by the gas flow. 展开更多
关键词 COAL dry cleaning DRYING DUST vibrated fluidized bed
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