Objective:To investigate the effect of exosomes secreted by decidual macrophages on trophoblast cells and their molecular mechanism.Methods:The decidual tissues of patients with preeclampsia(PE)and normal-term pregnan...Objective:To investigate the effect of exosomes secreted by decidual macrophages on trophoblast cells and their molecular mechanism.Methods:The decidual tissues of patients with preeclampsia(PE)and normal-term pregnant women were collected.Macrophages were obtained by the density gradient method and then flow cell sorting,then the exosomes were extracted.The structure of the exosomes was observed by transmission electron microscope.The expression of CD63,a marker protein of the exocrine body,was detected by western blot,and the exosomes were identified.CCK-8 was used to detect the effect of exosomes on trophoblast cell viability.Transwell migration experiment was used to detect the influence on migration ability.The expression of miR-146a-5p in exosomes was detected by qPCR.The effect of exosomes on the expression of HIF1αprotein in trophoblasts was detected by western blot and detection of the binding site between miR-146a-5p and HIF1αby double luciferase reporter gene was conducted.Results:The exosomes of macrophages present a"cake"structure with a middle depression about 30-130 nm in diameter,and CD63 is highly expressed,which conforms to the characteristics of exosomes.Compared with the normal group,the exosomes of decidual macrophages in the PE group inhibited the activity and migration of trophoblast cells(P<0.001).The expression of miR-146a-5p in the exosomes of decidual macrophages in the PE decreased significantly,and after exosomes of PE decidual macrophages treating trophoblast cells,the protein expression of HIF1αin trophoblast cells was significantly increased.There are targeted binding sites between miR-146a-5p and HIF1α.Conclusion:PE decidual macrophage exosomes can inhibit the viability and migration of trophoblast cells,which may be related to the decreased expression of miR-146a-5p in exosomes,thus promoting HIF1αprotein expression of trophoblast cells.展开更多
目的:采用系统评价的方法探讨培美曲塞联合顺铂(PC)对比吉西他滨联合顺铂(GC)治疗中国晚期初治非小细胞肺癌(NSCLC)的临床效果。方法:计算机检索PubMed、web of science、The Cochrane Library、CNKI、VIP、CBM、万方数据库收集PC对比G...目的:采用系统评价的方法探讨培美曲塞联合顺铂(PC)对比吉西他滨联合顺铂(GC)治疗中国晚期初治非小细胞肺癌(NSCLC)的临床效果。方法:计算机检索PubMed、web of science、The Cochrane Library、CNKI、VIP、CBM、万方数据库收集PC对比GC一线治疗晚期非小细胞肺癌的随机对照试验(RCT),根据Cochrane评价手册5.0版对纳入进行文献质量评价,采用Revman5.3软件对数据进行meta分析。结果:共纳入8个RCT,共801例患者。Meta分析结果显示PC方案的有效率明显提高(OR=1.28,95%CI:1.10-1.49,P=0.001);但亚组分析结果显示非鳞非小细胞癌组PC方案与GC方案疗效相似(P=0.05,OR=1.52,95%CI:1.00-2.30)。血小板减少的发生率、血红蛋白减少的发生率以及肝功能损害的发生率均显著降低。但两方案之间的疾病控制率、1年生存率、粒细胞减少发生率、恶心/呕吐发生率无显著差异。结论:PC方案一线治疗中国初治非鳞小细胞肺癌有效率与GC方案相当。但本次研究仍存在较多的局限性,两方案对比疗效如何,仍需更多的临床研究证实。展开更多
Background The activation of triggering receptor expressed on myeloid cells-1 （TREM-1） in the presence of microbial components amplifies the inflammatory response. The aim of the present study was to investigate the...Background The activation of triggering receptor expressed on myeloid cells-1 （TREM-1） in the presence of microbial components amplifies the inflammatory response. The aim of the present study was to investigate the effect of the modulation of the TREM-1 pathway during empyema in rats. Methods Adult male Wistar rats were subjected to empyema induced by intrapleural injection of Pseudomonas aeruginosa and Staphylococcus aureus. The animals were treated with LP17 （a synthetic TREM-1 inhibitor）, a control peptide, or a vehicle （normal saline）. Differential cell count, flow cytometry and histological examination were performed to evaluate local inflammatory alterations. Concentrations of tumor necrosis factor-a, interleukin-1B, and interleukin-6 in both pleural effusion and serum were measured by enzyme-linked immunosorbent assay. Results Although differential counts of each type of leukocytes in pleural effusion were not affected by LP17, a marked reduction in neutrophil numbers was seen in LP17 treated rats due to the reduction of both pleural effusion volume and total cell numbers. LP17 administration impaired concentration elevation in tumor necrosis factor-（], interleukin-1B, and interleukin-6 in both pleural effusion and serum. It was found that survival rate in LP17 treated rats was much higher than that in control rats. Conclusion The modulation of the TREM-1 pathway by the use of LP17 appears to be beneficial during empyema in rats in attenuating pleural and systemic inflammatory responses.展开更多
基金Hainan Provincial Natural Science Foundation Project(821MS128,822MS164)Hainan Provincial People's Hospital National Natural Science Foundation Cultivation Project(530)(2021MSXM04)。
文摘Objective:To investigate the effect of exosomes secreted by decidual macrophages on trophoblast cells and their molecular mechanism.Methods:The decidual tissues of patients with preeclampsia(PE)and normal-term pregnant women were collected.Macrophages were obtained by the density gradient method and then flow cell sorting,then the exosomes were extracted.The structure of the exosomes was observed by transmission electron microscope.The expression of CD63,a marker protein of the exocrine body,was detected by western blot,and the exosomes were identified.CCK-8 was used to detect the effect of exosomes on trophoblast cell viability.Transwell migration experiment was used to detect the influence on migration ability.The expression of miR-146a-5p in exosomes was detected by qPCR.The effect of exosomes on the expression of HIF1αprotein in trophoblasts was detected by western blot and detection of the binding site between miR-146a-5p and HIF1αby double luciferase reporter gene was conducted.Results:The exosomes of macrophages present a"cake"structure with a middle depression about 30-130 nm in diameter,and CD63 is highly expressed,which conforms to the characteristics of exosomes.Compared with the normal group,the exosomes of decidual macrophages in the PE group inhibited the activity and migration of trophoblast cells(P<0.001).The expression of miR-146a-5p in the exosomes of decidual macrophages in the PE decreased significantly,and after exosomes of PE decidual macrophages treating trophoblast cells,the protein expression of HIF1αin trophoblast cells was significantly increased.There are targeted binding sites between miR-146a-5p and HIF1α.Conclusion:PE decidual macrophage exosomes can inhibit the viability and migration of trophoblast cells,which may be related to the decreased expression of miR-146a-5p in exosomes,thus promoting HIF1αprotein expression of trophoblast cells.
文摘目的:采用系统评价的方法探讨培美曲塞联合顺铂(PC)对比吉西他滨联合顺铂(GC)治疗中国晚期初治非小细胞肺癌(NSCLC)的临床效果。方法:计算机检索PubMed、web of science、The Cochrane Library、CNKI、VIP、CBM、万方数据库收集PC对比GC一线治疗晚期非小细胞肺癌的随机对照试验(RCT),根据Cochrane评价手册5.0版对纳入进行文献质量评价,采用Revman5.3软件对数据进行meta分析。结果:共纳入8个RCT,共801例患者。Meta分析结果显示PC方案的有效率明显提高(OR=1.28,95%CI:1.10-1.49,P=0.001);但亚组分析结果显示非鳞非小细胞癌组PC方案与GC方案疗效相似(P=0.05,OR=1.52,95%CI:1.00-2.30)。血小板减少的发生率、血红蛋白减少的发生率以及肝功能损害的发生率均显著降低。但两方案之间的疾病控制率、1年生存率、粒细胞减少发生率、恶心/呕吐发生率无显著差异。结论:PC方案一线治疗中国初治非鳞小细胞肺癌有效率与GC方案相当。但本次研究仍存在较多的局限性,两方案对比疗效如何,仍需更多的临床研究证实。
基金This study was supported by a grant from National Science Fund for Distinguished Young Scholars （No. 30925032） and by grants from National Natural Science Foundation of China （No. 30872343 and No. 30770648）. None of the authors have a financial relationship with a commercial entity that has an interest in the subject of this manuscript.
文摘Background The activation of triggering receptor expressed on myeloid cells-1 （TREM-1） in the presence of microbial components amplifies the inflammatory response. The aim of the present study was to investigate the effect of the modulation of the TREM-1 pathway during empyema in rats. Methods Adult male Wistar rats were subjected to empyema induced by intrapleural injection of Pseudomonas aeruginosa and Staphylococcus aureus. The animals were treated with LP17 （a synthetic TREM-1 inhibitor）, a control peptide, or a vehicle （normal saline）. Differential cell count, flow cytometry and histological examination were performed to evaluate local inflammatory alterations. Concentrations of tumor necrosis factor-a, interleukin-1B, and interleukin-6 in both pleural effusion and serum were measured by enzyme-linked immunosorbent assay. Results Although differential counts of each type of leukocytes in pleural effusion were not affected by LP17, a marked reduction in neutrophil numbers was seen in LP17 treated rats due to the reduction of both pleural effusion volume and total cell numbers. LP17 administration impaired concentration elevation in tumor necrosis factor-（], interleukin-1B, and interleukin-6 in both pleural effusion and serum. It was found that survival rate in LP17 treated rats was much higher than that in control rats. Conclusion The modulation of the TREM-1 pathway by the use of LP17 appears to be beneficial during empyema in rats in attenuating pleural and systemic inflammatory responses.
