Objective To investigate the short- and long-term therapeutic efficacies of intravenous transplantation of bone marrow stem cells(MSCs) in rats with experimental myocardial infarction by metaanalysis.Methods Randomize...Objective To investigate the short- and long-term therapeutic efficacies of intravenous transplantation of bone marrow stem cells(MSCs) in rats with experimental myocardial infarction by metaanalysis.Methods Randomized controlled trials were systematically searched from Pub Med,Science Citation Index(SCI),Chinese journal full-text database(CJFD) up to December 2014.While the experimental groups(MSCs groups) were injected MSCs intravenously,the control groups were injected Delubecco's minimum essential medium(DMEM) or phosphate buffered saline(PBS).Subgroup analysis for each outcome measure was performed for the observing time point after the transplantation of MSCs.Weighted mean differences(WMD) and 95% confidence intervals(CI) were calculated for outcome parameters including ejection fraction(EF) and fractional shortening(FS),which were measured by echocardiogram after intravenous injection and analyzed by Rev Man 5.2 and STATA 12.0.Results Data from 9 studies(190 rats) were included in the meta-analysis.As compared to the control groups,the cardiac function of the experimental groups were not improved at day 7(EF:WMD=0.08,95%CI-1.32 to 1.16,P>0.01; FS:WMD=-0.12,95%CI-0.90 to 0.65,P>0.01) until at day 14 after MSCs' transplantation(EF:WMD=10.79,95%CI 9.16 to 12.42,P<0.01; FS:WMD=11.34,95%CI 10.44 to 12.23,P<0.01),and it lasted 4 weeks or more after transplantation of MSCs(EF:WMD=13.94,95%CI 12.24 to 15.64,P<0.01; FS:WMD=9.64,95%CI 7.98 to 11.31,P<0.01).Conclusion The therapeutic efficacies of MSCs in rats with myocardid infarction become increasing apparent as time advances since 2 weeks after injection.展开更多
In advanced hepatocellular carcinoma(HCC)tissues,M2-like tumor-associated macrophages(TAMs)are in the majority and promotes HCC progression.Contrary to the pro-tumor effect of M2-like TAMs,M1-like TAMs account for a s...In advanced hepatocellular carcinoma(HCC)tissues,M2-like tumor-associated macrophages(TAMs)are in the majority and promotes HCC progression.Contrary to the pro-tumor effect of M2-like TAMs,M1-like TAMs account for a small proportion and have anti-tumor effects.Since TAMs can switch from one type to another,reprogramming TAMs may be an important treatment for HCC therapy.However,the mechanisms of phenotypic switch and reprogramming TAMs are still obscure.In this study,we analyzed differential genes in normal macrophages and TAMs,and found that loss of MANF in TAMs accompanied by high levels of downstream genes negatively regulated by MANF.MANF reprogrammed TAMs into M1 phenotype.Meanwhile,loss of MANF promoted HCC progression in HCC patients and mice HCC model,especially tumor neovascularization.Additionally,macrophages with MANF supplement suppressed HCC progression in mice,suggesting MANF supplement in macrophage was an effective treatment for HCC.Mechanistically,MANF enhanced the HSF1-HSP70-1 interaction,restricted HSF1 in the cytoplasm of macrophages,and decreased both mRNA and protein levels of HSP70-1,which in turn led to reprogramming TAMs,and suppressing neovascularization of HCC.Our study contributes to the exploration the mechanism of TAMs reprogramming,which may provide insights for future therapeutic exploitation of HCC neovascularization.展开更多
Rubbers or elastomers play an important role in hi-tech technology and civilian daily life because of their unique and strategical properties.Generally,the rubber additives are essential components for rubbers’practi...Rubbers or elastomers play an important role in hi-tech technology and civilian daily life because of their unique and strategical properties.Generally,the rubber additives are essential components for rubbers’practical application.Nowadays,developing novel multifunctional additives has attracted increasing research attention.In this work,low-cost crude carbon dots(CCDs)were used as multifunctional additives for natural rubber/silica system(without any additional modification)through industrial compatible melt-mixing method.The results revealed that the CCDs could disperse well in the NR/silica system,and they could not only endow the rubber compound with excellent anti-aging capability due to CCDs’radical scavenging activity because of their plenty of nitrogen-containing species,but also improve the curing rate and mechanical performance of the rubber composite.Also,the CCDs could reduce the rolling resistance of the rubber composites(tanδvalue at 7%strain of the rubber composite could be decreased by 34%),which is promising for the application of energy-saving tire industry.Lastly,the addition of CCDs could effectively reduce the ZnO dosage by at least 40%in the rubber composite without deteriorating its performance.Overall,this work provides valuable guidance to develop novel cheap yet effective additives for the elastomer.展开更多
Slip boundary condition is commonly utilized to model elastic wave propagation through layered earth media. The same approach is used here to characterize acoustic wave propagation along a cased borehole with various ...Slip boundary condition is commonly utilized to model elastic wave propagation through layered earth media. The same approach is used here to characterize acoustic wave propagation along a cased borehole with various cement bond conditions. By modeling the cement layer between casing and formation as a viscoelastic slip interface with complex coupling rigidity parameters, one can not only reduce the complexity in the classical elastic wave modeling of the problem, but also efficiently model various complicated wave phenomena that are difficult for the existing modeling. More specifically, the new theory can well describe the effect of the cement bond condition change and the location of the change(i.e., whether it is in the first interface between casing and cement, or the second interface between cement and formation) on the acoustic waves,demonstrating the good modeling capability and predicting power. Application of the theory to field data shows that the theory can correctly model the acoustic wave characteristics and interpret the cement bond condition, thus providing a useful fundament theory for casing bond evaluation using acoustic logging.展开更多
Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study show...Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study showed that the serum from stressed mice presented a significant decrease in lymphocyte proliferation at 1, 12, 24, and 48 h after stress. There was not significant suppressive effect at 96 h. The half life of biological effect was 21.6展开更多
基金Supported by the Youth Project of National Natural Science Foundation(81100078)the Key Project of Chinese Ministry of Education(211207)+1 种基金Guangzhou Pearl River science and technology new star project plan(2012J2200063)Project of Guangdong Science and Technology Department(S2011040001392)
文摘Objective To investigate the short- and long-term therapeutic efficacies of intravenous transplantation of bone marrow stem cells(MSCs) in rats with experimental myocardial infarction by metaanalysis.Methods Randomized controlled trials were systematically searched from Pub Med,Science Citation Index(SCI),Chinese journal full-text database(CJFD) up to December 2014.While the experimental groups(MSCs groups) were injected MSCs intravenously,the control groups were injected Delubecco's minimum essential medium(DMEM) or phosphate buffered saline(PBS).Subgroup analysis for each outcome measure was performed for the observing time point after the transplantation of MSCs.Weighted mean differences(WMD) and 95% confidence intervals(CI) were calculated for outcome parameters including ejection fraction(EF) and fractional shortening(FS),which were measured by echocardiogram after intravenous injection and analyzed by Rev Man 5.2 and STATA 12.0.Results Data from 9 studies(190 rats) were included in the meta-analysis.As compared to the control groups,the cardiac function of the experimental groups were not improved at day 7(EF:WMD=0.08,95%CI-1.32 to 1.16,P>0.01; FS:WMD=-0.12,95%CI-0.90 to 0.65,P>0.01) until at day 14 after MSCs' transplantation(EF:WMD=10.79,95%CI 9.16 to 12.42,P<0.01; FS:WMD=11.34,95%CI 10.44 to 12.23,P<0.01),and it lasted 4 weeks or more after transplantation of MSCs(EF:WMD=13.94,95%CI 12.24 to 15.64,P<0.01; FS:WMD=9.64,95%CI 7.98 to 11.31,P<0.01).Conclusion The therapeutic efficacies of MSCs in rats with myocardid infarction become increasing apparent as time advances since 2 weeks after injection.
基金funded by support programs for Jun Liu,including the National Natural Science Foundation of China(82073862)Excellent Youth Talent Program of Anhui Province Natural Science Foundation(2108085Y27,China)funded by Anhui Province Natural Science Foundation(2208085MH284,China)for Xiangpeng Hu,and funded by the National Natural Science Foundation of China(U21A20345)for Yuxian Shen。
文摘In advanced hepatocellular carcinoma(HCC)tissues,M2-like tumor-associated macrophages(TAMs)are in the majority and promotes HCC progression.Contrary to the pro-tumor effect of M2-like TAMs,M1-like TAMs account for a small proportion and have anti-tumor effects.Since TAMs can switch from one type to another,reprogramming TAMs may be an important treatment for HCC therapy.However,the mechanisms of phenotypic switch and reprogramming TAMs are still obscure.In this study,we analyzed differential genes in normal macrophages and TAMs,and found that loss of MANF in TAMs accompanied by high levels of downstream genes negatively regulated by MANF.MANF reprogrammed TAMs into M1 phenotype.Meanwhile,loss of MANF promoted HCC progression in HCC patients and mice HCC model,especially tumor neovascularization.Additionally,macrophages with MANF supplement suppressed HCC progression in mice,suggesting MANF supplement in macrophage was an effective treatment for HCC.Mechanistically,MANF enhanced the HSF1-HSP70-1 interaction,restricted HSF1 in the cytoplasm of macrophages,and decreased both mRNA and protein levels of HSP70-1,which in turn led to reprogramming TAMs,and suppressing neovascularization of HCC.Our study contributes to the exploration the mechanism of TAMs reprogramming,which may provide insights for future therapeutic exploitation of HCC neovascularization.
基金supported by the National Natural Science Foundation of China(Nos.52103065,51988102 and 52273003)the scientific research fund of Wuhan Institute of Technology(No.K2021044).
文摘Rubbers or elastomers play an important role in hi-tech technology and civilian daily life because of their unique and strategical properties.Generally,the rubber additives are essential components for rubbers’practical application.Nowadays,developing novel multifunctional additives has attracted increasing research attention.In this work,low-cost crude carbon dots(CCDs)were used as multifunctional additives for natural rubber/silica system(without any additional modification)through industrial compatible melt-mixing method.The results revealed that the CCDs could disperse well in the NR/silica system,and they could not only endow the rubber compound with excellent anti-aging capability due to CCDs’radical scavenging activity because of their plenty of nitrogen-containing species,but also improve the curing rate and mechanical performance of the rubber composite.Also,the CCDs could reduce the rolling resistance of the rubber composites(tanδvalue at 7%strain of the rubber composite could be decreased by 34%),which is promising for the application of energy-saving tire industry.Lastly,the addition of CCDs could effectively reduce the ZnO dosage by at least 40%in the rubber composite without deteriorating its performance.Overall,this work provides valuable guidance to develop novel cheap yet effective additives for the elastomer.
基金supported by the National Natural Science Foundation of China (Grant No. 41774141)
文摘Slip boundary condition is commonly utilized to model elastic wave propagation through layered earth media. The same approach is used here to characterize acoustic wave propagation along a cased borehole with various cement bond conditions. By modeling the cement layer between casing and formation as a viscoelastic slip interface with complex coupling rigidity parameters, one can not only reduce the complexity in the classical elastic wave modeling of the problem, but also efficiently model various complicated wave phenomena that are difficult for the existing modeling. More specifically, the new theory can well describe the effect of the cement bond condition change and the location of the change(i.e., whether it is in the first interface between casing and cement, or the second interface between cement and formation) on the acoustic waves,demonstrating the good modeling capability and predicting power. Application of the theory to field data shows that the theory can correctly model the acoustic wave characteristics and interpret the cement bond condition, thus providing a useful fundament theory for casing bond evaluation using acoustic logging.
文摘Immuno-suppressive protein was generated in spleen and lymph node and released into serum in mice and rats under stress, which had strong suppressive effects on T and B lymphocyte proliferation. The present study showed that the serum from stressed mice presented a significant decrease in lymphocyte proliferation at 1, 12, 24, and 48 h after stress. There was not significant suppressive effect at 96 h. The half life of biological effect was 21.6
