Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of ...Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.展开更多
Small populations are at risk of extinction from deterministic and stochastic factors.Less than 250 Asian elephants(Elephas maximus)remain in China,and are distributed in a few isolated areas;yet,population viability ...Small populations are at risk of extinction from deterministic and stochastic factors.Less than 250 Asian elephants(Elephas maximus)remain in China,and are distributed in a few isolated areas;yet,population viability analyses of this endangered population have not been conducted.Here,the current genetic status of the Pu’Er-Mengyang Asian elephant populations in China was analyzed,and the risk of extinction was predicted over the next 500 years.Factors affecting the viability of this population were determined through simulations.The genetic diversity of the population was very low(mean allele number:3.1;expected heterozygosity:0.463),even though a recent population bottleneck was not detected.The effective population size was approximately 24.1 adult elephants.Enough adult breeding individuals exist to maintain population viability.VORTEX simulation model showed that this population would not go extinct in the next 500 years.However,illegal poaching and harvesting could negatively affect population size.A sensitivity analysis showed that the mean stochastic growth rate of the study population is sensitive to sex ratio,number of breeding females,mortality of females of different age classes,carrying capacity,and lethal equivalents.Based on our results,we suggest that action should be taken to alleviate inbreeding and any further loss of genetic diversity,by connecting fragmented elephant habitat or by translocating individual elephants.In addition,human–elephant conflict should be mitigated using various modern approaches,including crop guarding techniques,and by encouraging farmers to switch to crops and income sources not vulnerable to elephant raids.展开更多
基金This work was supported by National Natural Science Foundation of China(No.81360684)Guangxi Key Research and Development Plan Project(Gui Ke AB18221095)+3 种基金Teaching Teacher Training Project from Youjiang Medical University for Nationalities-National Teaching Teacher Training Project(You Hospital Zi[2018]No.98)High-level Talent Research Projects from Youjiang Medical University For Nationalities(No.01002018079)China National and Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599022)China Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599073).
文摘Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.
基金the National Nature Science Foundation of China[31570408]。
文摘Small populations are at risk of extinction from deterministic and stochastic factors.Less than 250 Asian elephants(Elephas maximus)remain in China,and are distributed in a few isolated areas;yet,population viability analyses of this endangered population have not been conducted.Here,the current genetic status of the Pu’Er-Mengyang Asian elephant populations in China was analyzed,and the risk of extinction was predicted over the next 500 years.Factors affecting the viability of this population were determined through simulations.The genetic diversity of the population was very low(mean allele number:3.1;expected heterozygosity:0.463),even though a recent population bottleneck was not detected.The effective population size was approximately 24.1 adult elephants.Enough adult breeding individuals exist to maintain population viability.VORTEX simulation model showed that this population would not go extinct in the next 500 years.However,illegal poaching and harvesting could negatively affect population size.A sensitivity analysis showed that the mean stochastic growth rate of the study population is sensitive to sex ratio,number of breeding females,mortality of females of different age classes,carrying capacity,and lethal equivalents.Based on our results,we suggest that action should be taken to alleviate inbreeding and any further loss of genetic diversity,by connecting fragmented elephant habitat or by translocating individual elephants.In addition,human–elephant conflict should be mitigated using various modern approaches,including crop guarding techniques,and by encouraging farmers to switch to crops and income sources not vulnerable to elephant raids.
