Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric...Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.展开更多
Introduction:Wetland soil fauna support material cycling and restoration processes in wetland ecosystems.In our study,we observed variations in wetland soil fauna on the shores of Lake Taihu,China.We examined the rela...Introduction:Wetland soil fauna support material cycling and restoration processes in wetland ecosystems.In our study,we observed variations in wetland soil fauna on the shores of Lake Taihu,China.We examined the relationships between fauna and major environmental factors,and looked at the short-and long-term changes in reed wetlands under restoration and in the natural reed lakeshore.Outcomes:We identified 93 groups of soil fauna in different wetlands and found significant differences in the lakeshore wetlands’soil fauna assemblages,depending on the length of the restoration period.By analyzing the soil fauna community evenness,dominance,number of taxa,and diversity,we found minimal seasonal variation in the soil fauna community diversity and abundance.The abundance of soil fauna in the sites under restoration decreased with depth below the soil surface.The reed restoration was obvious in the succession of the soil fauna groups in the long-term site.Although the restoration had an overall positive long-term effect on the soil fauna communities,there were no obvious short-term changes in the number of individuals.Conclusion:The study explored various potential measures to restore soil fauna in the Lake Taihu wetland and developed a theoretical basis for restoring the lakeshore wetland ecosystem.展开更多
Introduction:Plant communities and soil factors might interact with each other in different temporal and spatial scales,which can influence the patterns and processes of the wetland ecosystem.To get a better understan...Introduction:Plant communities and soil factors might interact with each other in different temporal and spatial scales,which can influence the patterns and processes of the wetland ecosystem.To get a better understanding of the distribution of plants in wetlands and analyze their associations with environmental soil factors,the structure and types of plant communities in the eastern shore area of Lake Taihu were analyzed by two-way indicator species analysis and canonical correspondence analysis(CCA)ordination.The spatial distribution patterns of vegetation and the main factors affecting the distributions were investigated.Outcomes:Sixty-six sampling sites were selected to obtain vegetation species and soil environmental factor data.Results showed that 22 species from the 66 sites could be divided into seven communities:I:Arundo donax;II:A.donax+Phragmites australis;III:Zizania latifolia+Typha orientalis;IV:P.australis+Alternanthera philoxeroides+Polygonum hydropiper;V:P.australis;VI:P.australis+Humulus scandens;and VII:Erigeron acer+Ipomoea batatas+Rumex acetosa.Plant species and soil factors in the CCA analysis showed that I.batatas,E.acer,Chenopodium album,Polygonum lapathifolium,and Acalypha australis were mainly affected by pH,whereas Echinochloa crus-galli,Setaria viridis,and H.scandens were mainly affected by soil total phosphorus.Mentha canadensis and A.donax were mainly affected by soil conductivity,A.philoxeroides was mainly affected by soil organic matter and,Z.latifolia,Metaplexis japonica and P.hydropiper were mainly affected by available phosphorus.Conclusion:These results indicated that different plants adapted to different soil environmental factors and provided basic information on the diversity of Lake Taihu wetland vegetation.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82201621(to LS),31930048(to QY)and 81720108016(to QY),and 81971225(to CG)the Key Research and Development Project of Shaanxi Province,No.2022SF-189(to XS)the Tangdu Hospital Supporting Foundation,Nos.2021ZTXM-006(to LS)and 2021JSZH-006(to CG)。
文摘Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.
基金The Special Fund for Forest Scientific Research in the Public Welfare[Grant Nos.201404305 and 200904001]“The Lecture and Study Program for Outstanding Scholars from Home and Abroad”[CAFYBB2011007]funded this research.
文摘Introduction:Wetland soil fauna support material cycling and restoration processes in wetland ecosystems.In our study,we observed variations in wetland soil fauna on the shores of Lake Taihu,China.We examined the relationships between fauna and major environmental factors,and looked at the short-and long-term changes in reed wetlands under restoration and in the natural reed lakeshore.Outcomes:We identified 93 groups of soil fauna in different wetlands and found significant differences in the lakeshore wetlands’soil fauna assemblages,depending on the length of the restoration period.By analyzing the soil fauna community evenness,dominance,number of taxa,and diversity,we found minimal seasonal variation in the soil fauna community diversity and abundance.The abundance of soil fauna in the sites under restoration decreased with depth below the soil surface.The reed restoration was obvious in the succession of the soil fauna groups in the long-term site.Although the restoration had an overall positive long-term effect on the soil fauna communities,there were no obvious short-term changes in the number of individuals.Conclusion:The study explored various potential measures to restore soil fauna in the Lake Taihu wetland and developed a theoretical basis for restoring the lakeshore wetland ecosystem.
基金The research in this article was funded by the Special Forestry Project of Public Interests[201404305,200904001].
文摘Introduction:Plant communities and soil factors might interact with each other in different temporal and spatial scales,which can influence the patterns and processes of the wetland ecosystem.To get a better understanding of the distribution of plants in wetlands and analyze their associations with environmental soil factors,the structure and types of plant communities in the eastern shore area of Lake Taihu were analyzed by two-way indicator species analysis and canonical correspondence analysis(CCA)ordination.The spatial distribution patterns of vegetation and the main factors affecting the distributions were investigated.Outcomes:Sixty-six sampling sites were selected to obtain vegetation species and soil environmental factor data.Results showed that 22 species from the 66 sites could be divided into seven communities:I:Arundo donax;II:A.donax+Phragmites australis;III:Zizania latifolia+Typha orientalis;IV:P.australis+Alternanthera philoxeroides+Polygonum hydropiper;V:P.australis;VI:P.australis+Humulus scandens;and VII:Erigeron acer+Ipomoea batatas+Rumex acetosa.Plant species and soil factors in the CCA analysis showed that I.batatas,E.acer,Chenopodium album,Polygonum lapathifolium,and Acalypha australis were mainly affected by pH,whereas Echinochloa crus-galli,Setaria viridis,and H.scandens were mainly affected by soil total phosphorus.Mentha canadensis and A.donax were mainly affected by soil conductivity,A.philoxeroides was mainly affected by soil organic matter and,Z.latifolia,Metaplexis japonica and P.hydropiper were mainly affected by available phosphorus.Conclusion:These results indicated that different plants adapted to different soil environmental factors and provided basic information on the diversity of Lake Taihu wetland vegetation.
