The natural gas hydrate has been regarded as an important future green energy.Significant progress on the hydrate exploitation has been made,but some challenges are still remaining.In order to enhance the hydrate expl...The natural gas hydrate has been regarded as an important future green energy.Significant progress on the hydrate exploitation has been made,but some challenges are still remaining.In order to enhance the hydrate exploitation efficiency,a significant understanding of the effective thermal conductivity(ETC)of the hydrate-bearing sediment has become essential,since it directly controls the heat and mass transfer behaviors,and thereby determines the stability of hydrate reservoir and production rate.In this study,the effective thermal conductivities of various hydrate-bearing sediments were in-situ measured and studied.The impacts of temperature,particle size and type of sediment were investigated.The effective thermal conductivities of the quartz sand sediments before and after hydrate formation were in-situ measured.The results show the weak negative correlation of effective thermal conductivity of the quartz sand sediment on the temperature before and after the hydrate formation.The effective thermal conductivity of the hydrate-bearing sediment decreases with the increase of particle size of the sediment.The dominant effect of the type of porous medium on the characteristics of the effective thermal conductivity of hydrate-bearing sediment was highlighted.The results indicate that both the effective thermal conductivities of hydrate-bearing quartz sand sediment and hydrate-bearing silicon carbide sediment are weakly negatively correlated with temperature,but the effective thermal conductivity of hydrate-bearing clay sediment is weakly positively dependent on the temperature.In addition,the values of the effective thermal conductivities of various hydrate-bearing sediments are in the order of hydrate-bearing silicon carbide sediment>hydrate-bearing quartz sand sediment>hydrate-bearing clay sediment.These findings could suggest that the intrinsic thermal conductivity of porous medium could control the characteristics of effective thermal conductivity of hydrate-bearing sediment.展开更多
Although zeolitic imidazolate frameworks(ZIFs)have bright prospects in wide fields like gas storage/separation,catalysis and medicine,etc.,their large-scale applications are bottlenecked by the absence of their low-co...Although zeolitic imidazolate frameworks(ZIFs)have bright prospects in wide fields like gas storage/separation,catalysis and medicine,etc.,their large-scale applications are bottlenecked by the absence of their low-cost commercial production technique.Here,we report an uncon ventional method suitable for environmentally friendly and low-cost mass-production of ZIFs.In this method,taking the synthesis of ZIF-8 as an example,ZnO was used instead of Zn(NO_(3))_(2) in traditional solvent synthesis methods and CO_(2) was introduced to dissolve ZnO in aqueous solution of 2-methylimidazole(HMeim)and form water soluble salt([ZnMeim]^(+)[MeimCOO]^(-))at room temperature.Then,by removing CO_(2) through heating or vacuuming,Meim-ions are produced and instantaneously assemble with[ZnMeim]^(+)s to generate ZIF-8 without any by product.Due to the absence of strong acid anions(such as NO^(-)_(3) and Cl^(-) et al.)in solution,the washing of filter cake required in the conventional approaches could be omitted and the filtrate containing only water and HMeim could be reused completely.This method is really green as no waste gas or liquid generates because CO_(2) and water could be recycled perfectly.It overcomes almost all bottlenecks occurred in commercial production of ZIF-8 when using traditional methods.A pilot plant was established for mass-production of ZIF-8 and hundreds kilograms of ZIF-8 was produced,which indicates that the new method is not only environmentally friendly but also low cost and commercial accessibility.It is expected that the new method would open an avenue for commercial applications of ZIFs.展开更多
Insights into the mechanism of hydrate nucleation are of great significance for the development of hydrate-based technologies,hydrate relevant flow assurance,and the exploration of in situ natural gas hydrates.Compare...Insights into the mechanism of hydrate nucleation are of great significance for the development of hydrate-based technologies,hydrate relevant flow assurance,and the exploration of in situ natural gas hydrates.Compared with the thermodynamics of hydrate formation,understanding the nucleation mechanism is challenging and has drawn substantial attention in recent decades.In this paper,we attempt to give a comprehensive review of the recent progress of studies of clathrate hydrate nucleation.First,the existing hypotheses on the hydrate nucleation mechanism are introduced and discussed.Then,we summarize recent experimental studies on induction time,a key parameter evaluating the velocity of the nucleation process.Subsequently,the memory effect is particularly discussed,followed by the suggestion of several promising research perspectives.展开更多
Coal bed methane has been considered as an important energy resource.One major difficulty of purifying coal bed methane comes from the similar physical properties of CH_4 and N_2.The ZIF-8/water-glycol slurry was used...Coal bed methane has been considered as an important energy resource.One major difficulty of purifying coal bed methane comes from the similar physical properties of CH_4 and N_2.The ZIF-8/water-glycol slurry was used as a medium to separate coal bed methane by fluidifying the solid adsorbent material.The sorption equilibrium experiment of binary mixture(CH_4/N_2)and slurry was conducted.The selectivity of CH_4 to N_2 is within the range of 2-6,which proved the feasibility of the slurry separation method.The modified Langmuir equation was used to describe the gas-slurry phase equilibrium behavior,and the calculated results were in good agreement with the experimental data.A continuous absorption-adsorption and desorption process on the separation of CH_4/N_2 in slurry is proposed and its mathematical model is also developed.Sensitivity analysis is conducted to determine the operation conditions and the energy performance of the proposed process was also evaluated.Feed gas contains 30 mol%of methane and the methane concentration in product gas is 95.46 mol%with the methane recovery ratio of 90.74%.The total energy consumption for per unit volume of product gas is determined as 1.846 kWh Nm^(-3).Experimental results and process simulation provide basic data for the design and operation of pilot and industrial plant.展开更多
In order to improve the performance of line spectrum detection,according to the feature that the underwater target radiated noise containing stable line spectrum,the differences of the phase difference between line sp...In order to improve the performance of line spectrum detection,according to the feature that the underwater target radiated noise containing stable line spectrum,the differences of the phase difference between line spectrum and background noise,a weighted line spectrum detection algorithm based on the phase variance is proposed in frequency domain.After phase difference alignment,the phase variance of line spectrum and the phase of background noise,respectively,are small and big in frequency domain,this method utilizes the weighted statistical algorithm to cumulate the frequency spectrum based on the phase variance,which can restrain the background noise disturbance,and enhance the signal to noise ratio(SNR).The theory analysis and experimental results both verify that the proposed method can well enhance the energy of line spectrum,restrain the energy of background noise,and have better detection performance under lower SNR.展开更多
In order to simulate the behavior of gas hydrate formation and decomposition,a 3-Dimension experimental device was built,consisting of a high-pressure reactor with an inner diameter of 300 mm,effective height of 100 m...In order to simulate the behavior of gas hydrate formation and decomposition,a 3-Dimension experimental device was built,consisting of a high-pressure reactor with an inner diameter of 300 mm,effective height of 100 mm,and operation pressure of 16 MPa.Eight thermal resistances were mounted in the porous media at different depthes and radiuses to detect the temperature distribution during the hydrate formation/decomposition.To collect the pressure,temperature,and flux of gas production data,the Monitor and Control Generated System(MCGS) was used.Using this device,the formation and decomposition behavior of methane hydrate in the 20 ～ 40 mesh natural sand with salinity of 3.35 wt% was examined.It was found that the front of formation or decomposition of hydrate can be judged by the temperature distribution.The amount of hydrate formation can also be evaluated by the temperature change.During the hydrate decomposition process,the temperature curves indicated that the hydrate in the top and bottom of reactor dissociated earlier than in the inner.The hydrate decomposition front gradually moved from porous media surface to inner and kept a shape of column form,with different moving speed at different surface position.The proper decomposition pressure was also determined.展开更多
Objective:Research has demonstrated that microRNA(miR)-106a is related to cisplatin resistance.We investigated the expression of miR-106a in the serum of patients with non-small cell lung cancer(NSCLC)and their sensit...Objective:Research has demonstrated that microRNA(miR)-106a is related to cisplatin resistance.We investigated the expression of miR-106a in the serum of patients with non-small cell lung cancer(NSCLC)and their sensitivity to chemotherapy by cisplatin combined with gemcitabine.Methods:Eighty-five NSCLC patients,who completed four cycles of gemcitabine and cisplatin chemotherapy,volunteered for this study and their serum samples were collected.Serum samples from 60 healthy subjects were used as controls.Real-time quantitative polymerase chain reaction(real-time q PCR)was used to quantify the level of miR-106a in the serum.Demographic and survival data of these patients were collected for the analysis.Results:The expression of miR-106a in the serum of NSCLC patients was significantly higher than that of healthy subjects(P<0.001).The expression of miR-106a was not correlated with patients'gender,age,tumor size,lymphatic metastasis,and pathological types;but was correlated with patients'tumor staging(P=0.003).After chemotherapy,serum miR-106a expression decreased in patients.The decrease in miR-106a expression in the chemotherapy-sensitive group was much higher than that in the chemotherapy-resistant group.Survival analysis shows that NSCLC patients with high expression of miR-106a have a poorer prognosis.The overall survival of NSCLC patients in the chemotherapy-sensitive group was significantly higher than that in the chemotherapy-resistant group.Conclusions:High expression of miR-106a may be involved in the development of NSCLC.Mi R-106a has significance in the prognosis of NSCLC.The level of miR-106a in the serum can be a useful parameter in screening for drug resistance during cisplatin-based chemotherapy.展开更多
Natural gas hydrate(NGH)is a highly efficient and clean energy,with huge reserves and widespread distribution in permafrost and marine areas.Researches all over the world are committed to developing an effective explo...Natural gas hydrate(NGH)is a highly efficient and clean energy,with huge reserves and widespread distribution in permafrost and marine areas.Researches all over the world are committed to developing an effective exploring technology for NGH reservoirs.In this paper,four conventional in-situ hydrate production methods,such as depressurization,thermal stimulation,inhibitor injection and CO2 replacement,are briefly introduced.Due to the limitations of each method,there has been no significantly breakthrough in hydrate exploring technology.Inspired by the development of unconventional oil and gas fields,researchers have put forward some new hydrate production methods.We summarize the enhanced hydrate exploiting methods,such as CO2/N2–CH4 replacement,CO2/H2–CH4 replacement,hydraulic fracturing treatment,and solid exploration;and potential hydrate mining techniques,such as self-generating heat fluid injection,geothermal stimulation,the well pattern optimization of hydrate exploring.The importance of reservoir stimulation technology for hydrate exploitation is emphasized,and it is believed that hydrate reservoir modification technology is a key to open hydrate resources exploitation,and the major challenges in the process of hydrate exploitation are pointed out.The combination of multiple hydrate exploring technologies and their complementary advantages will be the development trend in the future so as to promote the process of hydrate industrialization.展开更多
Low dosage kinetic hydrate inhibitors(KHIs)are a kind of alternative chemical additives to high dosage thermodynamic inhibitors for preventing gas hydrate formation in oil&gas production wells and transportation p...Low dosage kinetic hydrate inhibitors(KHIs)are a kind of alternative chemical additives to high dosage thermodynamic inhibitors for preventing gas hydrate formation in oil&gas production wells and transportation pipelines.In this paper,a new KHI,poly(N-vinyl caprolactam)-co-tert-butyl acrylate(PVCapco-TBA),was successfully synthesized with N-vinyl caprolactam(NVCap)and tert-butyl acrylate.The kinetic inhibition performances of PVCap-co-TBA on the formations of both structureⅠmethane hydrate and structureⅡnatural gas hydrate were investigated by measuring the onset times of hydrate formation under different conditions and compared with commercial KHIs such as PVP,PVCap and inhibex 501.The results indicated that PVCap-co-TBA outperformed these widely applied inhibitors for both structureⅠand structureⅡhydrates.At the same dosage of KHI,the maximum tolerable degree of subcooling under which the onset time of hydrate formation exceeded 24 hours for structureⅠhydrate was much lower than that for structureⅡhydrate.The inhibition strength increased with the increasing dosage of PVCap-co-TBA;The maximum tolerable degree of subcooling for the natural gas hydrate is more than10 K when the dosage was higher than 0.5%(mass)while it achieved 12 K when that dosage rose to0.75%(mass).Additionally,we found polypropylene glycol could be used as synergist at the dosage of 1.0%(mass)or so,under which the kinetic inhibition performance of PVCap-co-TBA could be improved significantly.All evaluation results demonstrated that PVCap-co-TBA was a very promising KHI and a competitive alternative to the existing commercial KHIs.展开更多
Carbon emission reduction and clean energy development are urgent demands for mankind in the coming decades.Exploring an efficient CO_(2) storage method can significantly reduce CO_(2) emissions in the short term.In t...Carbon emission reduction and clean energy development are urgent demands for mankind in the coming decades.Exploring an efficient CO_(2) storage method can significantly reduce CO_(2) emissions in the short term.In this study,we attempted to construct sediment samples with different residual CH_(4) hydrate amounts and reservoir conditions,and then investigate the potentials of both CO_(2) storage and enhanced CH_(4) recovery in depleted gas hydrate deposits in the permafrost and ocean zones,respectively.The results demonstrate that CO_(2) hydrate formation rate can be significantly improved due to the presence of residual hydrate seeds;However,excessive residual hydrates in turn lead to the decrease in CO_(2) storage efficiency.Affected by the T-P conditions of the reservoir,the storage amount of liquid CO_(2) can reach 8 times that of gaseous CO_(2),and CO_(2) stored in hydrate form reaches 2e4 times.Additionally,we noticed two other advantages of this method.One is that CO_(2) injection can enhance CH_(4) recovery rate and increases CH_(4) recovery by 10%e20%.The second is that hydrate saturation in the reservoir can be restored to 20%e40%,which means that the solid volume of the reservoir avoids serious shrinkage.Obviously,this is crucial for protecting the goaf stability.In summary,this approach is greatly promising for high-efficient CO_(2) storage and safe exploitation of gas hydrate.展开更多
To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and...To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.展开更多
Natural gas hydrate is an energy resource for methane that has a carbon quantity twice more than all traditional fossil fuels combined.However,their practical application in the field has been limited due to the chall...Natural gas hydrate is an energy resource for methane that has a carbon quantity twice more than all traditional fossil fuels combined.However,their practical application in the field has been limited due to the challenges of long-term preparation,high costs and associated risks.Experimental studies,on the other hand,offer a safe and cost-effective means of exploring the mechanisms of hydrate dissociation and optimizing exploitation conditions.Gas hydrate decomposition is a complicated process along with intrinsic kinetics,mass transfer and heat transfer,which are the influencing factors for hydrate decomposition rate.The identification of the rate-limiting factor for hydrate dissociation during depressurization varies with the scale of the reservoir,making it challenging to extrapolate findings from laboratory experiments to the actual exploitation.This review aims to summarize current knowledge of investigations on hydrate decomposition on the subject of the research scale(core scale,middle scale,large scale and field tests)and to analyze determining factors for decomposition rate,considering the various research scales and their associated influencing factors.展开更多
In order to obtain the robust high-resolution beamforming, a high order cross sensor processing(CSP) approach is developed. According to the relation ship between the target bearing and the phase difference of each el...In order to obtain the robust high-resolution beamforming, a high order cross sensor processing(CSP) approach is developed. According to the relation ship between the target bearing and the phase difference of each element receiving signal, this method exploits the property that the same diagonal of covariance matrix with the same phase difference and obtains(2M-1)(N-1)virtual elements(N is the original array number) by executing M order CSP. The extended virtual elements can effectively increase the physical aperture of linear array, reduce the main lobe width of beam-forming, and improve the bearing resolution. The CSP method accumulates the data on the same sub-diagonal of the covariance matrix, which can decrease the impact of background noise on beam-forming. The theoretical analysis and experimental results both show that this method has high resolution in bearing estimation, compared with the MUSIC method, which has better robustness under the lower signal-to-noise ratio(SNR).展开更多
基金This work was supported by the National Natural Science Foundation of China(U19B2005,21808238,U20B6005,22127812)the National Key Research and Development Program of China(2021YFC2800902).
文摘The natural gas hydrate has been regarded as an important future green energy.Significant progress on the hydrate exploitation has been made,but some challenges are still remaining.In order to enhance the hydrate exploitation efficiency,a significant understanding of the effective thermal conductivity(ETC)of the hydrate-bearing sediment has become essential,since it directly controls the heat and mass transfer behaviors,and thereby determines the stability of hydrate reservoir and production rate.In this study,the effective thermal conductivities of various hydrate-bearing sediments were in-situ measured and studied.The impacts of temperature,particle size and type of sediment were investigated.The effective thermal conductivities of the quartz sand sediments before and after hydrate formation were in-situ measured.The results show the weak negative correlation of effective thermal conductivity of the quartz sand sediment on the temperature before and after the hydrate formation.The effective thermal conductivity of the hydrate-bearing sediment decreases with the increase of particle size of the sediment.The dominant effect of the type of porous medium on the characteristics of the effective thermal conductivity of hydrate-bearing sediment was highlighted.The results indicate that both the effective thermal conductivities of hydrate-bearing quartz sand sediment and hydrate-bearing silicon carbide sediment are weakly negatively correlated with temperature,but the effective thermal conductivity of hydrate-bearing clay sediment is weakly positively dependent on the temperature.In addition,the values of the effective thermal conductivities of various hydrate-bearing sediments are in the order of hydrate-bearing silicon carbide sediment>hydrate-bearing quartz sand sediment>hydrate-bearing clay sediment.These findings could suggest that the intrinsic thermal conductivity of porous medium could control the characteristics of effective thermal conductivity of hydrate-bearing sediment.
基金supports received from the National Natural Science Foundation of China (21776301,21636009)are gratefully acknowledged.
文摘Although zeolitic imidazolate frameworks(ZIFs)have bright prospects in wide fields like gas storage/separation,catalysis and medicine,etc.,their large-scale applications are bottlenecked by the absence of their low-cost commercial production technique.Here,we report an uncon ventional method suitable for environmentally friendly and low-cost mass-production of ZIFs.In this method,taking the synthesis of ZIF-8 as an example,ZnO was used instead of Zn(NO_(3))_(2) in traditional solvent synthesis methods and CO_(2) was introduced to dissolve ZnO in aqueous solution of 2-methylimidazole(HMeim)and form water soluble salt([ZnMeim]^(+)[MeimCOO]^(-))at room temperature.Then,by removing CO_(2) through heating or vacuuming,Meim-ions are produced and instantaneously assemble with[ZnMeim]^(+)s to generate ZIF-8 without any by product.Due to the absence of strong acid anions(such as NO^(-)_(3) and Cl^(-) et al.)in solution,the washing of filter cake required in the conventional approaches could be omitted and the filtrate containing only water and HMeim could be reused completely.This method is really green as no waste gas or liquid generates because CO_(2) and water could be recycled perfectly.It overcomes almost all bottlenecks occurred in commercial production of ZIF-8 when using traditional methods.A pilot plant was established for mass-production of ZIF-8 and hundreds kilograms of ZIF-8 was produced,which indicates that the new method is not only environmentally friendly but also low cost and commercial accessibility.It is expected that the new method would open an avenue for commercial applications of ZIFs.
基金Supported by the National Key Research and Development Program of China(2016YFC0304003,2017YFC0307302)the National Natural Science Foundation of China(21636009,51576209,21522609)the Innovative Talents Support Program for Postgraduates(BX201700288)
文摘Insights into the mechanism of hydrate nucleation are of great significance for the development of hydrate-based technologies,hydrate relevant flow assurance,and the exploration of in situ natural gas hydrates.Compared with the thermodynamics of hydrate formation,understanding the nucleation mechanism is challenging and has drawn substantial attention in recent decades.In this paper,we attempt to give a comprehensive review of the recent progress of studies of clathrate hydrate nucleation.First,the existing hypotheses on the hydrate nucleation mechanism are introduced and discussed.Then,we summarize recent experimental studies on induction time,a key parameter evaluating the velocity of the nucleation process.Subsequently,the memory effect is particularly discussed,followed by the suggestion of several promising research perspectives.
基金The financial supports received from the National Natural Science Foundation of China(21522609,21636009 and 21878328)the National Key Research and Development Program of China(Nos.2017YFC0307302,2016YFC0304003)+1 种基金the Science Foundation of China University of Petroleum,Beijing(No.2462018BJC004)Beijing Science and Technology Program,China(No.Z181100005118010)。
文摘Coal bed methane has been considered as an important energy resource.One major difficulty of purifying coal bed methane comes from the similar physical properties of CH_4 and N_2.The ZIF-8/water-glycol slurry was used as a medium to separate coal bed methane by fluidifying the solid adsorbent material.The sorption equilibrium experiment of binary mixture(CH_4/N_2)and slurry was conducted.The selectivity of CH_4 to N_2 is within the range of 2-6,which proved the feasibility of the slurry separation method.The modified Langmuir equation was used to describe the gas-slurry phase equilibrium behavior,and the calculated results were in good agreement with the experimental data.A continuous absorption-adsorption and desorption process on the separation of CH_4/N_2 in slurry is proposed and its mathematical model is also developed.Sensitivity analysis is conducted to determine the operation conditions and the energy performance of the proposed process was also evaluated.Feed gas contains 30 mol%of methane and the methane concentration in product gas is 95.46 mol%with the methane recovery ratio of 90.74%.The total energy consumption for per unit volume of product gas is determined as 1.846 kWh Nm^(-3).Experimental results and process simulation provide basic data for the design and operation of pilot and industrial plant.
基金supported by the National Natural Science Foundation of China(61372180)the Young Talent Frontier Project of Institute of Acoustics of Chinese Academy of Sciences(Y454341261)
文摘In order to improve the performance of line spectrum detection,according to the feature that the underwater target radiated noise containing stable line spectrum,the differences of the phase difference between line spectrum and background noise,a weighted line spectrum detection algorithm based on the phase variance is proposed in frequency domain.After phase difference alignment,the phase variance of line spectrum and the phase of background noise,respectively,are small and big in frequency domain,this method utilizes the weighted statistical algorithm to cumulate the frequency spectrum based on the phase variance,which can restrain the background noise disturbance,and enhance the signal to noise ratio(SNR).The theory analysis and experimental results both verify that the proposed method can well enhance the energy of line spectrum,restrain the energy of background noise,and have better detection performance under lower SNR.
基金supported by the National Natural Science Foundation of China (Grant numbers: 20676145, U0633003)973 program (No. 2009CB219504)NCET-07-0842
文摘In order to simulate the behavior of gas hydrate formation and decomposition,a 3-Dimension experimental device was built,consisting of a high-pressure reactor with an inner diameter of 300 mm,effective height of 100 mm,and operation pressure of 16 MPa.Eight thermal resistances were mounted in the porous media at different depthes and radiuses to detect the temperature distribution during the hydrate formation/decomposition.To collect the pressure,temperature,and flux of gas production data,the Monitor and Control Generated System(MCGS) was used.Using this device,the formation and decomposition behavior of methane hydrate in the 20 ～ 40 mesh natural sand with salinity of 3.35 wt% was examined.It was found that the front of formation or decomposition of hydrate can be judged by the temperature distribution.The amount of hydrate formation can also be evaluated by the temperature change.During the hydrate decomposition process,the temperature curves indicated that the hydrate in the top and bottom of reactor dissociated earlier than in the inner.The hydrate decomposition front gradually moved from porous media surface to inner and kept a shape of column form,with different moving speed at different surface position.The proper decomposition pressure was also determined.
文摘Objective:Research has demonstrated that microRNA(miR)-106a is related to cisplatin resistance.We investigated the expression of miR-106a in the serum of patients with non-small cell lung cancer(NSCLC)and their sensitivity to chemotherapy by cisplatin combined with gemcitabine.Methods:Eighty-five NSCLC patients,who completed four cycles of gemcitabine and cisplatin chemotherapy,volunteered for this study and their serum samples were collected.Serum samples from 60 healthy subjects were used as controls.Real-time quantitative polymerase chain reaction(real-time q PCR)was used to quantify the level of miR-106a in the serum.Demographic and survival data of these patients were collected for the analysis.Results:The expression of miR-106a in the serum of NSCLC patients was significantly higher than that of healthy subjects(P<0.001).The expression of miR-106a was not correlated with patients'gender,age,tumor size,lymphatic metastasis,and pathological types;but was correlated with patients'tumor staging(P=0.003).After chemotherapy,serum miR-106a expression decreased in patients.The decrease in miR-106a expression in the chemotherapy-sensitive group was much higher than that in the chemotherapy-resistant group.Survival analysis shows that NSCLC patients with high expression of miR-106a have a poorer prognosis.The overall survival of NSCLC patients in the chemotherapy-sensitive group was significantly higher than that in the chemotherapy-resistant group.Conclusions:High expression of miR-106a may be involved in the development of NSCLC.Mi R-106a has significance in the prognosis of NSCLC.The level of miR-106a in the serum can be a useful parameter in screening for drug resistance during cisplatin-based chemotherapy.
基金Supported by the National Key Research and Development Program of China(2017YFC0307302,2016YFC0304003)the National Natural Science Foundation of China(21636009,51576209,51676207,21522609)
文摘Natural gas hydrate(NGH)is a highly efficient and clean energy,with huge reserves and widespread distribution in permafrost and marine areas.Researches all over the world are committed to developing an effective exploring technology for NGH reservoirs.In this paper,four conventional in-situ hydrate production methods,such as depressurization,thermal stimulation,inhibitor injection and CO2 replacement,are briefly introduced.Due to the limitations of each method,there has been no significantly breakthrough in hydrate exploring technology.Inspired by the development of unconventional oil and gas fields,researchers have put forward some new hydrate production methods.We summarize the enhanced hydrate exploiting methods,such as CO2/N2–CH4 replacement,CO2/H2–CH4 replacement,hydraulic fracturing treatment,and solid exploration;and potential hydrate mining techniques,such as self-generating heat fluid injection,geothermal stimulation,the well pattern optimization of hydrate exploring.The importance of reservoir stimulation technology for hydrate exploitation is emphasized,and it is believed that hydrate reservoir modification technology is a key to open hydrate resources exploitation,and the major challenges in the process of hydrate exploitation are pointed out.The combination of multiple hydrate exploring technologies and their complementary advantages will be the development trend in the future so as to promote the process of hydrate industrialization.
基金supported by the National Natural Science Foundation of China(U20B6005)Hainan Province Science and Technology Special Fund(ZDKJ2021026)。
文摘Low dosage kinetic hydrate inhibitors(KHIs)are a kind of alternative chemical additives to high dosage thermodynamic inhibitors for preventing gas hydrate formation in oil&gas production wells and transportation pipelines.In this paper,a new KHI,poly(N-vinyl caprolactam)-co-tert-butyl acrylate(PVCapco-TBA),was successfully synthesized with N-vinyl caprolactam(NVCap)and tert-butyl acrylate.The kinetic inhibition performances of PVCap-co-TBA on the formations of both structureⅠmethane hydrate and structureⅡnatural gas hydrate were investigated by measuring the onset times of hydrate formation under different conditions and compared with commercial KHIs such as PVP,PVCap and inhibex 501.The results indicated that PVCap-co-TBA outperformed these widely applied inhibitors for both structureⅠand structureⅡhydrates.At the same dosage of KHI,the maximum tolerable degree of subcooling under which the onset time of hydrate formation exceeded 24 hours for structureⅠhydrate was much lower than that for structureⅡhydrate.The inhibition strength increased with the increasing dosage of PVCap-co-TBA;The maximum tolerable degree of subcooling for the natural gas hydrate is more than10 K when the dosage was higher than 0.5%(mass)while it achieved 12 K when that dosage rose to0.75%(mass).Additionally,we found polypropylene glycol could be used as synergist at the dosage of 1.0%(mass)or so,under which the kinetic inhibition performance of PVCap-co-TBA could be improved significantly.All evaluation results demonstrated that PVCap-co-TBA was a very promising KHI and a competitive alternative to the existing commercial KHIs.
基金financially supported by the National Natural Science Foundation of China,China(22378424,52004136,22127812,U20B6005)the Science Foundation of China University of Petroleum Beijing(2462023BJRC017)Hunan Provincial Department of Education Scientific Research Project(22B0310).
文摘Carbon emission reduction and clean energy development are urgent demands for mankind in the coming decades.Exploring an efficient CO_(2) storage method can significantly reduce CO_(2) emissions in the short term.In this study,we attempted to construct sediment samples with different residual CH_(4) hydrate amounts and reservoir conditions,and then investigate the potentials of both CO_(2) storage and enhanced CH_(4) recovery in depleted gas hydrate deposits in the permafrost and ocean zones,respectively.The results demonstrate that CO_(2) hydrate formation rate can be significantly improved due to the presence of residual hydrate seeds;However,excessive residual hydrates in turn lead to the decrease in CO_(2) storage efficiency.Affected by the T-P conditions of the reservoir,the storage amount of liquid CO_(2) can reach 8 times that of gaseous CO_(2),and CO_(2) stored in hydrate form reaches 2e4 times.Additionally,we noticed two other advantages of this method.One is that CO_(2) injection can enhance CH_(4) recovery rate and increases CH_(4) recovery by 10%e20%.The second is that hydrate saturation in the reservoir can be restored to 20%e40%,which means that the solid volume of the reservoir avoids serious shrinkage.Obviously,this is crucial for protecting the goaf stability.In summary,this approach is greatly promising for high-efficient CO_(2) storage and safe exploitation of gas hydrate.
基金supports received from National Natural Science Foundation of China(U20B6005,22178378,and 22127812)are gratefully acknowledgedIn addition,Kun Li wants to thank,in particular,the great support and encourage from Shuangshuang Li.Looking forward to a bright future with you.
文摘To date,the primary industrial carbon capture approach is still absorption using aqueous solutions of alkanolamines.Here,to pursue a substitute for the amine-based approach to improve the CO_(2) capture efficiency and decrease the energy cost further,we report a new carbon capture approach using a 2-methylimidazole(mIm)aqueous solution.The properties and sorption behaviors of this approach have been experimentally investigated.The results show that the mIm solution has higher CO_(2) absorption capacity under relatively higher equilibrium pressure(>130 kPa)and lower desorption heat than the methyldiethanolamine solution.91.6%sorption capacity of mIm solution can be recovered at 353.15 K and 80 kPa.The selectivity for CO_(2)/N_(2) and CO_(2)/CH_(4) can reach an exceptional 7609 and 4324,respectively.Furthermore,the pilot-scale tests were also performed,and the results demonstrate that more than 98%of CO_(2) in the feed gas could be removed and cyclic absorption capacity can reach 1 mol·L^(-1).This work indicates that mIm is an excellent alternative to alkanolamines for carbon capture in the industry.
基金Financial support received from the National Natural Science Foundation of China(22178379)the National Key Research and Development Program of China(2021YFC2800902)is gratefully acknowledged.
文摘Natural gas hydrate is an energy resource for methane that has a carbon quantity twice more than all traditional fossil fuels combined.However,their practical application in the field has been limited due to the challenges of long-term preparation,high costs and associated risks.Experimental studies,on the other hand,offer a safe and cost-effective means of exploring the mechanisms of hydrate dissociation and optimizing exploitation conditions.Gas hydrate decomposition is a complicated process along with intrinsic kinetics,mass transfer and heat transfer,which are the influencing factors for hydrate decomposition rate.The identification of the rate-limiting factor for hydrate dissociation during depressurization varies with the scale of the reservoir,making it challenging to extrapolate findings from laboratory experiments to the actual exploitation.This review aims to summarize current knowledge of investigations on hydrate decomposition on the subject of the research scale(core scale,middle scale,large scale and field tests)and to analyze determining factors for decomposition rate,considering the various research scales and their associated influencing factors.
基金supported by the National Natural Science Foundation of China(61372180)the Young Talent Frontier Project of Institute of Acoustics of Chinese Academy of Sciences(Y454341261)
文摘In order to obtain the robust high-resolution beamforming, a high order cross sensor processing(CSP) approach is developed. According to the relation ship between the target bearing and the phase difference of each element receiving signal, this method exploits the property that the same diagonal of covariance matrix with the same phase difference and obtains(2M-1)(N-1)virtual elements(N is the original array number) by executing M order CSP. The extended virtual elements can effectively increase the physical aperture of linear array, reduce the main lobe width of beam-forming, and improve the bearing resolution. The CSP method accumulates the data on the same sub-diagonal of the covariance matrix, which can decrease the impact of background noise on beam-forming. The theoretical analysis and experimental results both show that this method has high resolution in bearing estimation, compared with the MUSIC method, which has better robustness under the lower signal-to-noise ratio(SNR).
