Enhancing the active tumor targeting ability and decreasing the clearance of reticuloendothelial system(RES)are important issues for drug delivery systems(DDSs)in cancer therapy.In recent years,cell membrane camouflag...Enhancing the active tumor targeting ability and decreasing the clearance of reticuloendothelial system(RES)are important issues for drug delivery systems(DDSs)in cancer therapy.In recent years,cell membrane camouflage,as one of the biomimetic modification strategies,has shown huge potential.Many natural properties of source cells can be inherited,allowing the DDSs to successfully avoid phagocytosis by macrophages,prolong circulation time,and achieve homologous targeting to lesion tissue.In this study,a cancer cell membrane camouflaged nanoplatform based on gelatin with a typical core-shell structure was developed for cancer chemotherapy.Doxorubicin(DOX)loaded gelatin nanogel(NG@DOX)acted as the inner core,and 4T1(mouse breast carcinoma cell)membrane was set as the outer shell(M-NG@DOX).The M-NG platform enhanced the ability of homologous targeting due to the surface protein of cell membrane being completely retained,which could promote the cell uptake of homotypic cells,avoid phagocytosis by RAW264.7 macrophages,and therefore increase accumulation in tumor tissue.Meanwhile,due to the better controlled drug release capability of M-NG@DOX,premature release of DOX in circulation could be reduced,minimizing side effects in common chemotherapy.As a result,the biomimetic nanoplatform in this study,obtained by a cancer cell membrane camouflaged drug delivery system,efficiently reached desirable tumor elimination,providing a significant strategy for effective targeted therapy and specific carcinoma therapy.展开更多
A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomi...A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomimetic peptide and vascular endothelial growth factor-165(VEGF) that achieved controlled release, cell attachment, proliferation and differentiation. To promote osteogenesis with VEGF, we designed the BMP-2 biomimetic peptide that comprised BMP-2 core sequence oligopeptide(SSVPT), phosphoserine, and synthetic cell adhesion factor(RGDS). In vitro cell experiments, the scaffold was conducive to the adhesion and proliferation of rat bone marrow mesenchymal stem cells(r BMSCs). The micro-CT3 D reconstruction of the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center area implanted with the prepared cryogel, and tissue section staining analysis demonstrated that the scaffold with double-growth factor can synergistically accelerate bone regeneration. These findings suggested that the obtained gelatin cryogel could serve as a cell-responsive platform for biomaterial-based nonbearing bone repair.展开更多
基金financially supported by the Scientific Research Start-up Funds of Hexi University(KYQD2022004)the 13th Innovation Program Science and Technology for college students of Hexi University(138)。
基金supported by grants from Science and Technology Program of Guangzhou,China(No.201804010146)Guangzhou Science and Technology Program City-University Joint Funding Project(No.2023A03J0001)。
文摘Enhancing the active tumor targeting ability and decreasing the clearance of reticuloendothelial system(RES)are important issues for drug delivery systems(DDSs)in cancer therapy.In recent years,cell membrane camouflage,as one of the biomimetic modification strategies,has shown huge potential.Many natural properties of source cells can be inherited,allowing the DDSs to successfully avoid phagocytosis by macrophages,prolong circulation time,and achieve homologous targeting to lesion tissue.In this study,a cancer cell membrane camouflaged nanoplatform based on gelatin with a typical core-shell structure was developed for cancer chemotherapy.Doxorubicin(DOX)loaded gelatin nanogel(NG@DOX)acted as the inner core,and 4T1(mouse breast carcinoma cell)membrane was set as the outer shell(M-NG@DOX).The M-NG platform enhanced the ability of homologous targeting due to the surface protein of cell membrane being completely retained,which could promote the cell uptake of homotypic cells,avoid phagocytosis by RAW264.7 macrophages,and therefore increase accumulation in tumor tissue.Meanwhile,due to the better controlled drug release capability of M-NG@DOX,premature release of DOX in circulation could be reduced,minimizing side effects in common chemotherapy.As a result,the biomimetic nanoplatform in this study,obtained by a cancer cell membrane camouflaged drug delivery system,efficiently reached desirable tumor elimination,providing a significant strategy for effective targeted therapy and specific carcinoma therapy.
基金supported by grants from Science and Technology Program of Guangzhou,China (No. 201804010146)Natural Science Foundation of Guangdong Province,China (No.2016A030313341)+2 种基金Science and Technology Planning Project of Guangdong Province,China (No. 2014B020215001)National Natural Science Foundation of China (No. 51973243)Science and Technology Planning Project of Shenzhen (No. JCYJ20190807155801657)。
文摘A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomimetic peptide and vascular endothelial growth factor-165(VEGF) that achieved controlled release, cell attachment, proliferation and differentiation. To promote osteogenesis with VEGF, we designed the BMP-2 biomimetic peptide that comprised BMP-2 core sequence oligopeptide(SSVPT), phosphoserine, and synthetic cell adhesion factor(RGDS). In vitro cell experiments, the scaffold was conducive to the adhesion and proliferation of rat bone marrow mesenchymal stem cells(r BMSCs). The micro-CT3 D reconstruction of the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center area implanted with the prepared cryogel, and tissue section staining analysis demonstrated that the scaffold with double-growth factor can synergistically accelerate bone regeneration. These findings suggested that the obtained gelatin cryogel could serve as a cell-responsive platform for biomaterial-based nonbearing bone repair.
