Background:Physical activity(PA)is generally encouraged.Studies from developed countries in the West have shown that maintenance of adequate PA or increasing PA are associated with lower mortality risk.It is unclear w...Background:Physical activity(PA)is generally encouraged.Studies from developed countries in the West have shown that maintenance of adequate PA or increasing PA are associated with lower mortality risk.It is unclear whether these associations apply to an older Chinese population.Hence,we examined the changes in PA prospectively among a middle-aged and older Chinese population over an average of 4 years and explored their subsequent mortality risks.Methods:Metabolic equivalent scores of PA among participants in the Guangzhou Biobank Cohort Study were calculated.Participants were divided into 3 groups related to PA level,and changes in PA were classified into 9 categories.Information on vital status and causes of death from March 2008 to December 2012(the first repeated examination)until December 31,2017,was obtained via record linkage with the Death Registry.Results:Of 18,104 participants aged 61.21±6.85 years(mean±SD),1461 deaths occurred within 141,417 person-years.Compared to participants who maintained moderate PA,those who decreased PA from moderate or high levels to a low level had increased risks for all-cause mortality(hazard ratio(HR)=1.47,95%confidence interval(95%CI):1.11-1.96).Participants who maintained a high level of PA(HR=0.83,95%CI:0.70-0.98)or increased PA from low to high levels(HR=0.71,95%CI:0.52-0.97)showed lower all-cause mortality risks.Those who maintained low PA levels showed a higher all-cause mortality risk,whereas those who increased their PA levels showed a non-significantly lower risk.Similar results were found for cardiovascular disease risk.Conclusion:Even at an older age,maintaining a high PA level or increasing PA from low to high levels results in lower mortality risks,suggesting that substantial health benefits might be achieved by maintaining or increasing engagement in adequate levels of PA.The increased risk of maintaining a low PA level or decreasing PA to a low level warrants the attention of public health officials and clinicians.展开更多
pH, exchangeable Ca2+ and Mg2+ of soil can influence the yield and quality of flue-cured tobacco. Xuancheng city is the dominant tobacco-planting region in Anhui province since 2008. A general soil survey was conducte...pH, exchangeable Ca2+ and Mg2+ of soil can influence the yield and quality of flue-cured tobacco. Xuancheng city is the dominant tobacco-planting region in Anhui province since 2008. A general soil survey was conducted in Xuancheng city to understand the current situations of pH, exchangeable Ca2+ and Mg2+ of farmlands under tobacco-rice rotation and to decide whether continuous applying dolomite powders to abate soil acidification. In total 124 topsoil samples (0-20 cm) were collected from the typical farmlands and soil pH, exchangeable Ca2+ and Mg2+ were measured. The results showed that soil pH and Ca2+ and Mg2+ in Xuancheng city were generally in the suitable grades, pH ranged from 4.56 to 8.42 with an average of 5.87, exchangeable Ca2+ ranged from 1.01 cmol(1/2Ca2+) kg-1 to 100.55 cmol(1/2Ca2+) kg-1 with an average of 11.07 cmol(1/2Ca2+) kg-1, and exchangeable Mg2+ ranged from 0.14 cmol(1/2Mg2+) kg-1 to 2.86 cmol(1/2Mg2+) kg-1 with an average of 0.81 cmol(1/2Mg2+) kg-1, all spanned from the low grades to the high grades. To the whole Xuancheng city, 37.10%, 62.10% and 72.58% of the surveyed farmlands were in the low grades of pH, exchangeable Ca2+ and Mg2+, respectively, while 52.42%, 25.00% and 20.97% of the farmlands were in the suitable grades, respectively. pH had an extremely significantly positive Napierian logarithm correlation with exchangeable Ca2+ and an extremely significantly positive power correlation with exchangeable Mg2+. In total about 338.2 kg·hm-2 of Ca2+ and 202.4 kg·hm-2 of Mg2+ are annually input into the farmlands, while about 110.4 kg·hm-2 of Ca2+ and 7.4 kg·hm-2 of Mg2+ are annually moved out from the farmlands, exchangeable Ca2+ and Mg2+ were annually increased by 225.2 kg·hm-2 and 187.6 kg·hm-2 in the farmlands, respectively. It is necessary to monitor the farmlands dynamically in order to decide whether continuous applying dolomite powders which should be dependent upon the specific conditions of individual farmlands.展开更多
A better understanding of nitrogen (N) diffusion and transformation in soils could reveal the capacity of the biological inorganic N and improve the efficiency of N fertilizers. A field micro-plot experiment was carri...A better understanding of nitrogen (N) diffusion and transformation in soils could reveal the capacity of the biological inorganic N and improve the efficiency of N fertilizers. A field micro-plot experiment was carried out to study the effects of fertilization methods (mixed uniformly with 12 cm top soil, placed in holes at a 12-cm depth, or placed in furrows at a 12-cm depth) and forms of N fertilizers (urea and ammonium phosphate) on the dynamics of soil N’s vertical diffusion and horizontal migration in red-yellow soil. The soil inorganic N ( NH4+-N and NH3--N) content following point deep placement was greater than that from mixed or furrow applications. Under point placement, the migration of soil inorganic N in urea and ammonium phosphate treatments occurred in the 6 - 15 cm layer at a horizontal distance of 0 - 9 cm. However, the nutrient preservation capability of the soil receiving ammonium phosphate was greater than that receiving urea under point deep placement. Thus, point deep placement had a tendency to increase the inorganic N in the soil and reduce inorganic N loss, which probably occurred due to the reduced soil volume with which the N fertilizer was mixed. According to crop growth and fertilizer requirements, the optimized fertilizer placement and N species resulted in a continuously high nutrient supply to crops for 90 d. However, the effects of point deep placement on increasing the N-use efficiency and reducing N loss have to be evaluated under natural field conditions.展开更多
The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2）...The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2） solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine （BA） and α-naphthaleneacetic acid （NAA） added to Murashige and Skoog （MS） medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips （96.7%, 9.8） and leaves （90.0%, 8.7） were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid （IBA） with the highest rooting frequency （93.3%） and numbers of roots/explant （8.2）. For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat （1： 1）. After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana ＇Neva＇.展开更多
Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen （N） supply （low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil） on the growth, photosynthetic chara...Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen （N） supply （low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil） on the growth, photosynthetic characteristics and photosynthetic nitrogen use efficiency （PNUE） of tobacco seedlings （Nicotiana tabacum L. Yunyan 87）. The results showed middle and high N significantly enhanced seedling growth including plant stem and leaf dry weight comparing with low N. High N supply could lead to a dramatic increase in the photosynthetic capacity of tobacco seedlings under low N conditions. There were significant differences in leaf N content between nitrogen treatments. About a 76% increase in leafN content in plants fed by high N resulted in about 43% increase in Rubisco content and 27% in net photosynthetic rate. The non-corresponding increases in photosynthetic rate in tobacco seedlings fed by high N relative to low N resulted from Rubisco activity and/or carboxylation efficiency （CE）. These results indicated that tobacco seedlings under high N application can maintain high net photosynthetic rate （Pn） but lower PNUE, will finally result in a decline in N use efficiency.展开更多
Xuancheng City is the dominant tobacco-planting area of Anhui province due to the high quality of the tobacco leaves. However, the potassium content in the tobacco leaves shows a gradual decreasing trend in recent yea...Xuancheng City is the dominant tobacco-planting area of Anhui province due to the high quality of the tobacco leaves. However, the potassium content in the tobacco leaves shows a gradual decreasing trend in recent years, which may be attributed partly to the possible low potassium content in topsoil of tobacco-planting farmland. Therefore, the content data in the year of 2005-2007 of rapidly available potassium (RA-K) of 7730 topsoil samples mainly under double rice rotation or wheat/rice-rice rotation at that time and the content data of slowly available potassium (SA-K) and RA-K of 124 typical topsoil samples in the year of 2015 under tobacco-rice rotation were used and compared in order to disclose the status of topsoil potassium and to provide a guidance for reasonable potassium fertilization in Xuancheng. The results showed that in 2005-2007 RA-K content ranged from 1 mg·kg-1 to 844 mg·kg-1 with an average of 68 mg·kg-1, and 82.7% of topsoil samples were insufficient in RA-K (-1). Comparatively in 2015 SA-K content ranged from 230 mg·kg-1 to 1340 mg·kg-1 with an average of 595 mg·kg-1, and 13.7% of soil samples were insufficient in SA-K (-1);RA-K content ranged from 46 mg·kg-1 to 352 mg·kg-1 with an average of 134 mg·kg-1, and 25.8% of soil samples were insufficient in RA-K (-1). The above data show that RA-K content has increased gradually in farmland topsoil mainly due to fertilization since 1980s, particularly to farmland under tobacco-rice rotation, but insufficient RA-K in topsoil is still widely and serious for farmland under double rice rotation or wheat/rice-rice rotation. Under tobacco-rice rotation, RA-K insufficiency usually occurred in the farmlands which plant tobacco less than 3 years, indicating more potassium fertilizer should be applied.展开更多
The available contents of microelements in the topsoil can influence the yield and quality of flue-cured tobacco. Xuancheng is the dominant tobacco-planting region in Anhui province since 2008. In this paper, the tops...The available contents of microelements in the topsoil can influence the yield and quality of flue-cured tobacco. Xuancheng is the dominant tobacco-planting region in Anhui province since 2008. In this paper, the topsoil available Fe, Mn, Cu and Zn contents of 4197 farmlands mainly under rice-rice rotation and wheat-rice rotation in 2008 and of 124 typical farmlands under tobacco-rice rotation in 2015 in Xuancheng city were analyzed in order to disclose the changes and spatial distributions of these microelements and to instruct the reasonable application of the microelement fertilizers. The results showed that the topsoil average available contents in the farmlands under rice-rice rotation or wheat-rice rotation in 2008 were 105.9 mg·kg-1 for Fe, 19.6 mg·kg-1 for Mn, 4.5 mg·kg-1 for Cu, and 3.0 mg·kg-1 for Zn, respectively, increased by 33.14%, 64.29%, 51.11% and 36.67%, respectively, compared with those in the 2nd Soil Survey in 1980s, and the historic, once intensive and overall application of microelement fertilizers was attributed to the great increases. The topsoil average available contents in the farmlands under tobacco-rice rotation in 2015 were 31.1 mg·kg-1 for Fe, 9.8 mg·kg-1 for Mn, 2.1 mg·kg-1 for Cu, and 0.3 mg·kg-1 for Zn, respectively, decreased by 70.63%, 50.00%, 53.33% and 90.00%, respectively, compared with those of the farmlands under rice-rice rotation or wheat-rice rotation in 2008, and the net deficiencies in the input and output of microelements were attributed to the significant decreases in the topsoil microelements. It is necessary to monitor the farmlands under tobacco-rice rotation dynamically and in time in order to decide whether applying microelement fertilizers or not.展开更多
基金supported by the National Natural Science Foundation (No.81941019)the National Key R&D Program of China (No.2017YFC0907100)+3 种基金the Natural Science Foundation of Guangdong (No.2018A030313140)the Guangzhou Science and Technology Bureau,Guangzhou,China (No.201704030132)the Major Infectious Disease Prevention and Control of the National Science and Technique Major Project (No.2018ZX10715004)the University of Birmingham,UK
文摘Background:Physical activity(PA)is generally encouraged.Studies from developed countries in the West have shown that maintenance of adequate PA or increasing PA are associated with lower mortality risk.It is unclear whether these associations apply to an older Chinese population.Hence,we examined the changes in PA prospectively among a middle-aged and older Chinese population over an average of 4 years and explored their subsequent mortality risks.Methods:Metabolic equivalent scores of PA among participants in the Guangzhou Biobank Cohort Study were calculated.Participants were divided into 3 groups related to PA level,and changes in PA were classified into 9 categories.Information on vital status and causes of death from March 2008 to December 2012(the first repeated examination)until December 31,2017,was obtained via record linkage with the Death Registry.Results:Of 18,104 participants aged 61.21±6.85 years(mean±SD),1461 deaths occurred within 141,417 person-years.Compared to participants who maintained moderate PA,those who decreased PA from moderate or high levels to a low level had increased risks for all-cause mortality(hazard ratio(HR)=1.47,95%confidence interval(95%CI):1.11-1.96).Participants who maintained a high level of PA(HR=0.83,95%CI:0.70-0.98)or increased PA from low to high levels(HR=0.71,95%CI:0.52-0.97)showed lower all-cause mortality risks.Those who maintained low PA levels showed a higher all-cause mortality risk,whereas those who increased their PA levels showed a non-significantly lower risk.Similar results were found for cardiovascular disease risk.Conclusion:Even at an older age,maintaining a high PA level or increasing PA from low to high levels results in lower mortality risks,suggesting that substantial health benefits might be achieved by maintaining or increasing engagement in adequate levels of PA.The increased risk of maintaining a low PA level or decreasing PA to a low level warrants the attention of public health officials and clinicians.
文摘pH, exchangeable Ca2+ and Mg2+ of soil can influence the yield and quality of flue-cured tobacco. Xuancheng city is the dominant tobacco-planting region in Anhui province since 2008. A general soil survey was conducted in Xuancheng city to understand the current situations of pH, exchangeable Ca2+ and Mg2+ of farmlands under tobacco-rice rotation and to decide whether continuous applying dolomite powders to abate soil acidification. In total 124 topsoil samples (0-20 cm) were collected from the typical farmlands and soil pH, exchangeable Ca2+ and Mg2+ were measured. The results showed that soil pH and Ca2+ and Mg2+ in Xuancheng city were generally in the suitable grades, pH ranged from 4.56 to 8.42 with an average of 5.87, exchangeable Ca2+ ranged from 1.01 cmol(1/2Ca2+) kg-1 to 100.55 cmol(1/2Ca2+) kg-1 with an average of 11.07 cmol(1/2Ca2+) kg-1, and exchangeable Mg2+ ranged from 0.14 cmol(1/2Mg2+) kg-1 to 2.86 cmol(1/2Mg2+) kg-1 with an average of 0.81 cmol(1/2Mg2+) kg-1, all spanned from the low grades to the high grades. To the whole Xuancheng city, 37.10%, 62.10% and 72.58% of the surveyed farmlands were in the low grades of pH, exchangeable Ca2+ and Mg2+, respectively, while 52.42%, 25.00% and 20.97% of the farmlands were in the suitable grades, respectively. pH had an extremely significantly positive Napierian logarithm correlation with exchangeable Ca2+ and an extremely significantly positive power correlation with exchangeable Mg2+. In total about 338.2 kg·hm-2 of Ca2+ and 202.4 kg·hm-2 of Mg2+ are annually input into the farmlands, while about 110.4 kg·hm-2 of Ca2+ and 7.4 kg·hm-2 of Mg2+ are annually moved out from the farmlands, exchangeable Ca2+ and Mg2+ were annually increased by 225.2 kg·hm-2 and 187.6 kg·hm-2 in the farmlands, respectively. It is necessary to monitor the farmlands dynamically in order to decide whether continuous applying dolomite powders which should be dependent upon the specific conditions of individual farmlands.
文摘A better understanding of nitrogen (N) diffusion and transformation in soils could reveal the capacity of the biological inorganic N and improve the efficiency of N fertilizers. A field micro-plot experiment was carried out to study the effects of fertilization methods (mixed uniformly with 12 cm top soil, placed in holes at a 12-cm depth, or placed in furrows at a 12-cm depth) and forms of N fertilizers (urea and ammonium phosphate) on the dynamics of soil N’s vertical diffusion and horizontal migration in red-yellow soil. The soil inorganic N ( NH4+-N and NH3--N) content following point deep placement was greater than that from mixed or furrow applications. Under point placement, the migration of soil inorganic N in urea and ammonium phosphate treatments occurred in the 6 - 15 cm layer at a horizontal distance of 0 - 9 cm. However, the nutrient preservation capability of the soil receiving ammonium phosphate was greater than that receiving urea under point deep placement. Thus, point deep placement had a tendency to increase the inorganic N in the soil and reduce inorganic N loss, which probably occurred due to the reduced soil volume with which the N fertilizer was mixed. According to crop growth and fertilizer requirements, the optimized fertilizer placement and N species resulted in a continuously high nutrient supply to crops for 90 d. However, the effects of point deep placement on increasing the N-use efficiency and reducing N loss have to be evaluated under natural field conditions.
文摘The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ＇Neva＇ by using in vitro shoots tips and leaves. For sterilization, 0.1% （w/v） mercuric chloride （HgCl2） solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine （BA） and α-naphthaleneacetic acid （NAA） added to Murashige and Skoog （MS） medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips （96.7%, 9.8） and leaves （90.0%, 8.7） were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid （IBA） with the highest rooting frequency （93.3%） and numbers of roots/explant （8.2）. For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat （1： 1）. After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus×euramericana ＇Neva＇.
文摘Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen （N） supply （low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil） on the growth, photosynthetic characteristics and photosynthetic nitrogen use efficiency （PNUE） of tobacco seedlings （Nicotiana tabacum L. Yunyan 87）. The results showed middle and high N significantly enhanced seedling growth including plant stem and leaf dry weight comparing with low N. High N supply could lead to a dramatic increase in the photosynthetic capacity of tobacco seedlings under low N conditions. There were significant differences in leaf N content between nitrogen treatments. About a 76% increase in leafN content in plants fed by high N resulted in about 43% increase in Rubisco content and 27% in net photosynthetic rate. The non-corresponding increases in photosynthetic rate in tobacco seedlings fed by high N relative to low N resulted from Rubisco activity and/or carboxylation efficiency （CE）. These results indicated that tobacco seedlings under high N application can maintain high net photosynthetic rate （Pn） but lower PNUE, will finally result in a decline in N use efficiency.
文摘Xuancheng City is the dominant tobacco-planting area of Anhui province due to the high quality of the tobacco leaves. However, the potassium content in the tobacco leaves shows a gradual decreasing trend in recent years, which may be attributed partly to the possible low potassium content in topsoil of tobacco-planting farmland. Therefore, the content data in the year of 2005-2007 of rapidly available potassium (RA-K) of 7730 topsoil samples mainly under double rice rotation or wheat/rice-rice rotation at that time and the content data of slowly available potassium (SA-K) and RA-K of 124 typical topsoil samples in the year of 2015 under tobacco-rice rotation were used and compared in order to disclose the status of topsoil potassium and to provide a guidance for reasonable potassium fertilization in Xuancheng. The results showed that in 2005-2007 RA-K content ranged from 1 mg·kg-1 to 844 mg·kg-1 with an average of 68 mg·kg-1, and 82.7% of topsoil samples were insufficient in RA-K (-1). Comparatively in 2015 SA-K content ranged from 230 mg·kg-1 to 1340 mg·kg-1 with an average of 595 mg·kg-1, and 13.7% of soil samples were insufficient in SA-K (-1);RA-K content ranged from 46 mg·kg-1 to 352 mg·kg-1 with an average of 134 mg·kg-1, and 25.8% of soil samples were insufficient in RA-K (-1). The above data show that RA-K content has increased gradually in farmland topsoil mainly due to fertilization since 1980s, particularly to farmland under tobacco-rice rotation, but insufficient RA-K in topsoil is still widely and serious for farmland under double rice rotation or wheat/rice-rice rotation. Under tobacco-rice rotation, RA-K insufficiency usually occurred in the farmlands which plant tobacco less than 3 years, indicating more potassium fertilizer should be applied.
文摘The available contents of microelements in the topsoil can influence the yield and quality of flue-cured tobacco. Xuancheng is the dominant tobacco-planting region in Anhui province since 2008. In this paper, the topsoil available Fe, Mn, Cu and Zn contents of 4197 farmlands mainly under rice-rice rotation and wheat-rice rotation in 2008 and of 124 typical farmlands under tobacco-rice rotation in 2015 in Xuancheng city were analyzed in order to disclose the changes and spatial distributions of these microelements and to instruct the reasonable application of the microelement fertilizers. The results showed that the topsoil average available contents in the farmlands under rice-rice rotation or wheat-rice rotation in 2008 were 105.9 mg·kg-1 for Fe, 19.6 mg·kg-1 for Mn, 4.5 mg·kg-1 for Cu, and 3.0 mg·kg-1 for Zn, respectively, increased by 33.14%, 64.29%, 51.11% and 36.67%, respectively, compared with those in the 2nd Soil Survey in 1980s, and the historic, once intensive and overall application of microelement fertilizers was attributed to the great increases. The topsoil average available contents in the farmlands under tobacco-rice rotation in 2015 were 31.1 mg·kg-1 for Fe, 9.8 mg·kg-1 for Mn, 2.1 mg·kg-1 for Cu, and 0.3 mg·kg-1 for Zn, respectively, decreased by 70.63%, 50.00%, 53.33% and 90.00%, respectively, compared with those of the farmlands under rice-rice rotation or wheat-rice rotation in 2008, and the net deficiencies in the input and output of microelements were attributed to the significant decreases in the topsoil microelements. It is necessary to monitor the farmlands under tobacco-rice rotation dynamically and in time in order to decide whether applying microelement fertilizers or not.
