Alzheimer’s disease is a prevalent and debilitating neurodegenerative condition that profoundly affects a patient’s daily functioning with progressive cognitive decline,which can be partly attributed to impaired hip...Alzheimer’s disease is a prevalent and debilitating neurodegenerative condition that profoundly affects a patient’s daily functioning with progressive cognitive decline,which can be partly attributed to impaired hippocampal neurogenesis.Neurogenesis in the hippocampal dentate gyrus is likely to persist throughout life but declines with aging,especially in Alzheimer’s disease.Recent evidence indicated that RNA-binding protein 8A(Rbm8a)promotes the proliferation of neural progenitor cells,with lower expression levels observed in Alzheimer’s disease patients compared with healthy people.This study investigated the hypothesis that Rbm8a overexpression may enhance neurogenesis by promoting the proliferation of neural progenitor cells to improve memory impairment in Alzheimer’s disease.Therefore,Rbm8a overexpression was induced in the dentate gyrus of 5×FAD mice to validate this hypothesis.Elevated Rbm8a levels in the dentate gyrus triggered neurogenesis and abated pathological phenotypes(such as plaque formation,gliosis reaction,and dystrophic neurites),leading to ameliorated memory performance in 5×FAD mice.RNA sequencing data further substantiated these findings,showing the enrichment of differentially expressed genes involved in biological processes including neurogenesis,cell proliferation,and amyloid protein formation.In conclusion,overexpressing Rbm8a in the dentate gyrus of 5×FAD mouse brains improved cognitive function by ameliorating amyloid-beta-associated pathological phenotypes and enhancing neurogenesis.展开更多
To assess whether a development strategy will be profitable enough,production forecasting is a crucial and difficult step in the process.The development history of other reservoirs in the same class tends to be studie...To assess whether a development strategy will be profitable enough,production forecasting is a crucial and difficult step in the process.The development history of other reservoirs in the same class tends to be studied to make predictions accurate.However,the permeability field,well patterns,and development regime must all be similar for two reservoirs to be considered in the same class.This results in very few available experiences from other reservoirs even though there is a lot of historical information on numerous reservoirs because it is difficult to find such similar reservoirs.This paper proposes a learn-to-learn method,which can better utilize a vast amount of historical data from various reservoirs.Intuitively,the proposed method first learns how to learn samples before directly learning rules in samples.Technically,by utilizing gradients from networks with independent parameters and copied structure in each class of reservoirs,the proposed network obtains the optimal shared initial parameters which are regarded as transferable information across different classes.Based on that,the network is able to predict future production indices for the target reservoir by only training with very limited samples collected from reservoirs in the same class.Two cases further demonstrate its superiority in accuracy to other widely-used network methods.展开更多
Objective This study aimed to investigate the changes of follicular helper T(TFH)and follicular regulatory T(TFR)cell subpopulations in patients with non-small cell lung cancer(NSCLC)and their significance.Methods Per...Objective This study aimed to investigate the changes of follicular helper T(TFH)and follicular regulatory T(TFR)cell subpopulations in patients with non-small cell lung cancer(NSCLC)and their significance.Methods Peripheral blood was collected from 58 NSCLC patients at different stages and 38 healthy controls.Flow cytometry was used to detect TFH cell subpopulation based on programmed death 1(PD-1)and inducible co-stimulator(ICOS),and TFR cell subpopulation based on cluster determinant 45RA(CD45RA)and forkhead box protein P3(FoxP3).The levels of interleukin-10(IL-10),interleukin-17a(IL-17a),interleukin-21(IL-21),and transforming growth factor-β(TGF-β)in the plasma were measured,and changes in circulating B cell subsets and plasma IgG levels were also analyzed.The correlation between serum cytokeratin fragment antigen 21-1(CYFRA 21-1)levels and TFH,TFR,or B cell subpopulations was further explored.Results The TFR/TFH ratio increased significantly in NSCLC patients.The CD45RA^(+)FoxP3^(int) TFR subsets were increased,with their proportions increasing in stages Ⅱ to Ⅲ and decreasing in stage IV.PD-1^(+)ICOS+TFH cells showed a downward trend with increasing stages.Plasma IL-21 and TGF-β concentrations were increased in NSCLC patients compared with healthy controls.Plasmablasts,plasma IgG levels,and CD45RA^(+)FoxP3^(int) TFR cells showed similar trends.TFH numbers and plasmablasts were positively correlated with CYFRA 21-1 in stages Ⅰ-Ⅲ and negatively correlated with CYFRA 21-1 in stage IV.Conclusion Circulating TFH and TFR cell subpopulations and plasmablasts dynamically change in different stages of NSCLC,which is associated with serum CYFRA 21-1 levels and reflects disease progression.展开更多
目的探讨“互联网+医疗”模式在肺癌术后肺康复患者的应用效果。方法选取笔者医院2022年6月至2023年1月接受择期肺癌手术治疗的87例肺癌患者,按随机数字表法分为对照组44例与观察组43例;对照组行常规干预,观察组行基于“互联网+医疗”...目的探讨“互联网+医疗”模式在肺癌术后肺康复患者的应用效果。方法选取笔者医院2022年6月至2023年1月接受择期肺癌手术治疗的87例肺癌患者,按随机数字表法分为对照组44例与观察组43例;对照组行常规干预,观察组行基于“互联网+医疗”模式干预;对比两组肺功能指标[用力肺活量(forced vital capacity,FVC)、第1秒用力肺活量(first second forced vital capacity,FEV1)、呼吸流量(respiratory flow,PEF)]、心肺耐力水平[6min步行试验(6min walking test,6MWT)]、呼吸劳累感及生活质量[世界卫生组织生存质量测定简表(World Health Organization quality of life,WHOQOL-BREF)]。结果干预6个月,研究组FVC、FEV1、PEF、6MWT距离、WHOQOL-BREE评分均较对照组高,呼吸疲劳感评分较对照组低,差异有统计学意义(P<0.05)。结论肺癌术后康复患者采用“互联网+医疗”模式干预可改善肺功能,提高心肺耐力,减轻呼吸疲劳感,提升生活质量。展开更多
目的比较电针手部经筋结点疗法与电针传统穴位疗法治疗脑卒中后(痉挛期)手运动功能障碍的临床疗效差异。方法纳入64例脑卒中后痉挛期手运动功能障碍患者,随机分为经筋结点组和传统穴位组,每组32例,其中经筋结点组脱落1例,传统穴位组脱落...目的比较电针手部经筋结点疗法与电针传统穴位疗法治疗脑卒中后(痉挛期)手运动功能障碍的临床疗效差异。方法纳入64例脑卒中后痉挛期手运动功能障碍患者,随机分为经筋结点组和传统穴位组,每组32例,其中经筋结点组脱落1例,传统穴位组脱落2例。两组均加电针,每次治疗30 min,每天2次,每周6 d,共4周。经筋结点组针刺指伸肌结点和指外展结点,传统穴位组针刺外关和合谷、阳池、后溪、大陵。通过改良Ashworth痉挛评定量表(modified Ashworth scale,MAS)、临床神经功能缺损程度评分、腕手部关节主动活动度(active range of motion,AROM)、简化Fugl-Meyer运动功能评分法(Fugl-Meyer assessment,FMA)(手部分)进行疗效判定。结果治疗后经筋结点组和传统穴位组AROM、FMA较治疗前均有提高(P<0.05),临床神经功能缺损程度、MAS评分均较治疗前降低(P<0.01,P<0.05),且治疗后经筋结点组MAS、AROM、FMA、临床神经功能缺损程度评分均优于传统穴位组(P<0.05);电针针刺经筋结点组总有效率为87%(27/31),电针传统穴位组总有效率为70%(21/30),两组比较结果有统计学意义(P<0.05)。结论电针手部经筋结点疗法对脑卒中后痉挛期手运动功能障碍治疗有效,且疗效优于电针传统穴位疗法。展开更多
基金supported by the National Natural Science Foundation of China,No.91849104(to YW)。
文摘Alzheimer’s disease is a prevalent and debilitating neurodegenerative condition that profoundly affects a patient’s daily functioning with progressive cognitive decline,which can be partly attributed to impaired hippocampal neurogenesis.Neurogenesis in the hippocampal dentate gyrus is likely to persist throughout life but declines with aging,especially in Alzheimer’s disease.Recent evidence indicated that RNA-binding protein 8A(Rbm8a)promotes the proliferation of neural progenitor cells,with lower expression levels observed in Alzheimer’s disease patients compared with healthy people.This study investigated the hypothesis that Rbm8a overexpression may enhance neurogenesis by promoting the proliferation of neural progenitor cells to improve memory impairment in Alzheimer’s disease.Therefore,Rbm8a overexpression was induced in the dentate gyrus of 5×FAD mice to validate this hypothesis.Elevated Rbm8a levels in the dentate gyrus triggered neurogenesis and abated pathological phenotypes(such as plaque formation,gliosis reaction,and dystrophic neurites),leading to ameliorated memory performance in 5×FAD mice.RNA sequencing data further substantiated these findings,showing the enrichment of differentially expressed genes involved in biological processes including neurogenesis,cell proliferation,and amyloid protein formation.In conclusion,overexpressing Rbm8a in the dentate gyrus of 5×FAD mouse brains improved cognitive function by ameliorating amyloid-beta-associated pathological phenotypes and enhancing neurogenesis.
基金This work is supported by the National Natural Science Foundation of China under Grant 52274057,52074340 and 51874335the Major Scientific and Technological Projects of CNPC under Grant ZD2019-183-008+2 种基金the Major Scientific and Technological Projects of CNOOC under Grant CCL2022RCPS0397RSNthe Science and Technology Support Plan for Youth Innovation of University in Shandong Province under Grant 2019KJH002111 Project under Grant B08028.
文摘To assess whether a development strategy will be profitable enough,production forecasting is a crucial and difficult step in the process.The development history of other reservoirs in the same class tends to be studied to make predictions accurate.However,the permeability field,well patterns,and development regime must all be similar for two reservoirs to be considered in the same class.This results in very few available experiences from other reservoirs even though there is a lot of historical information on numerous reservoirs because it is difficult to find such similar reservoirs.This paper proposes a learn-to-learn method,which can better utilize a vast amount of historical data from various reservoirs.Intuitively,the proposed method first learns how to learn samples before directly learning rules in samples.Technically,by utilizing gradients from networks with independent parameters and copied structure in each class of reservoirs,the proposed network obtains the optimal shared initial parameters which are regarded as transferable information across different classes.Based on that,the network is able to predict future production indices for the target reservoir by only training with very limited samples collected from reservoirs in the same class.Two cases further demonstrate its superiority in accuracy to other widely-used network methods.
基金supported by grants from the National Natural Science Foundation of China(No.82271755,No.81871230)Peking University People's Hospital Scientific Research Development Funds(RZ 2022-06).
文摘Objective This study aimed to investigate the changes of follicular helper T(TFH)and follicular regulatory T(TFR)cell subpopulations in patients with non-small cell lung cancer(NSCLC)and their significance.Methods Peripheral blood was collected from 58 NSCLC patients at different stages and 38 healthy controls.Flow cytometry was used to detect TFH cell subpopulation based on programmed death 1(PD-1)and inducible co-stimulator(ICOS),and TFR cell subpopulation based on cluster determinant 45RA(CD45RA)and forkhead box protein P3(FoxP3).The levels of interleukin-10(IL-10),interleukin-17a(IL-17a),interleukin-21(IL-21),and transforming growth factor-β(TGF-β)in the plasma were measured,and changes in circulating B cell subsets and plasma IgG levels were also analyzed.The correlation between serum cytokeratin fragment antigen 21-1(CYFRA 21-1)levels and TFH,TFR,or B cell subpopulations was further explored.Results The TFR/TFH ratio increased significantly in NSCLC patients.The CD45RA^(+)FoxP3^(int) TFR subsets were increased,with their proportions increasing in stages Ⅱ to Ⅲ and decreasing in stage IV.PD-1^(+)ICOS+TFH cells showed a downward trend with increasing stages.Plasma IL-21 and TGF-β concentrations were increased in NSCLC patients compared with healthy controls.Plasmablasts,plasma IgG levels,and CD45RA^(+)FoxP3^(int) TFR cells showed similar trends.TFH numbers and plasmablasts were positively correlated with CYFRA 21-1 in stages Ⅰ-Ⅲ and negatively correlated with CYFRA 21-1 in stage IV.Conclusion Circulating TFH and TFR cell subpopulations and plasmablasts dynamically change in different stages of NSCLC,which is associated with serum CYFRA 21-1 levels and reflects disease progression.
文摘目的探讨“互联网+医疗”模式在肺癌术后肺康复患者的应用效果。方法选取笔者医院2022年6月至2023年1月接受择期肺癌手术治疗的87例肺癌患者,按随机数字表法分为对照组44例与观察组43例;对照组行常规干预,观察组行基于“互联网+医疗”模式干预;对比两组肺功能指标[用力肺活量(forced vital capacity,FVC)、第1秒用力肺活量(first second forced vital capacity,FEV1)、呼吸流量(respiratory flow,PEF)]、心肺耐力水平[6min步行试验(6min walking test,6MWT)]、呼吸劳累感及生活质量[世界卫生组织生存质量测定简表(World Health Organization quality of life,WHOQOL-BREF)]。结果干预6个月,研究组FVC、FEV1、PEF、6MWT距离、WHOQOL-BREE评分均较对照组高,呼吸疲劳感评分较对照组低,差异有统计学意义(P<0.05)。结论肺癌术后康复患者采用“互联网+医疗”模式干预可改善肺功能,提高心肺耐力,减轻呼吸疲劳感,提升生活质量。
基金funded by the National Science and Technology Major Project,China(Nos.2017VI-0002-0072,2019-VI-0020-0135)the National Natural Science Foundation of China(Nos.51771148,52071263)+4 种基金the Key Research and Development Program of Shaanxi Province,China(No.2020ZDLGY13-02)the Natural Science Basic Research Plan in Shaanxi Province,China(No.2021JC-13)the Research Fund of the State Key Laboratory of Solidification Processing(NPU),China(No.2021-QZ-03)the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University,China(No.CX2021057)the Science,Technology,and Innovation Commission of Shenzhen Municipality,China(No.JCYJ20180306171121424)。
文摘目的比较电针手部经筋结点疗法与电针传统穴位疗法治疗脑卒中后(痉挛期)手运动功能障碍的临床疗效差异。方法纳入64例脑卒中后痉挛期手运动功能障碍患者,随机分为经筋结点组和传统穴位组,每组32例,其中经筋结点组脱落1例,传统穴位组脱落2例。两组均加电针,每次治疗30 min,每天2次,每周6 d,共4周。经筋结点组针刺指伸肌结点和指外展结点,传统穴位组针刺外关和合谷、阳池、后溪、大陵。通过改良Ashworth痉挛评定量表(modified Ashworth scale,MAS)、临床神经功能缺损程度评分、腕手部关节主动活动度(active range of motion,AROM)、简化Fugl-Meyer运动功能评分法(Fugl-Meyer assessment,FMA)(手部分)进行疗效判定。结果治疗后经筋结点组和传统穴位组AROM、FMA较治疗前均有提高(P<0.05),临床神经功能缺损程度、MAS评分均较治疗前降低(P<0.01,P<0.05),且治疗后经筋结点组MAS、AROM、FMA、临床神经功能缺损程度评分均优于传统穴位组(P<0.05);电针针刺经筋结点组总有效率为87%(27/31),电针传统穴位组总有效率为70%(21/30),两组比较结果有统计学意义(P<0.05)。结论电针手部经筋结点疗法对脑卒中后痉挛期手运动功能障碍治疗有效,且疗效优于电针传统穴位疗法。
