Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four diff erent categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.T...Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four diff erent categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.Their abilities to produce protease,amylase,and lipase were determined,and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions.The purifi cation of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate,and sequentially chromatographed by anion exchange column and gel filtration.The purifi ed protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE.The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80℃and a wide acidic range of pH 6.5-11.5,and displayed highest activity at 40℃ and pH 8.5.Notably,the protease still maintained high activity even at low to 10℃.Furthermore,the protease exhibited good stability in presence of diff erent surfactants,organic solvents,oxidizing agent H_(2)O_(2),and commercial detergents.Therefore,the protease produced by gcc-1 is a cold active and high stable enzyme,and has a promising potential in laundry detergent as an additive.展开更多
To fabricate an oil-water separation material that is rich in source,eco-friendly,and responsive,in this study,we successfully developed a collagen-based sponge for application to oil-water separation based on a green...To fabricate an oil-water separation material that is rich in source,eco-friendly,and responsive,in this study,we successfully developed a collagen-based sponge for application to oil-water separation based on a green and facile strategy.In this design,widely-available collagen(COL)was used as the substrate:it was immersed in polydimethylsiloxane(PDMS)suspension with candle soot(CS)nanoparticles,followed by hot curing.The resultant sponge(CS/PDMS-COL)possessed good hydrophobicity with a water contact angle of 148.3°under a low PDMS concentration of 2%.The results from field emission scanning electron microscope,Fourier transform infrared spectrometer,X-ray photoelectron spectrometer,and X-ray diffractometry demonstrated the successful coating of CS and PDMS on the surface of COL substrate.The CS/PDMS-COL can adsorb eight oils,with the adsorption capacity for trichloromethane reaching 95 g/g.With benzene as the target adsorbent,the separation efficiency was maintained at no less than 95%even after recycling 20 times.CS/PDMS-COL was also used to separate oil-in-water emulsion.Moreover,the sponge killed bacteria effectively due to its excellent near-infrared photothermal responsiveness.This study provides new insight into the preparation of facile oil-water separation materials based on naturally occurring biomaterials effortlessly.展开更多
The design and development of energy storage device with high energy/power density has become a research hotspot.Zinc-ion hybrid capacitors(ZHCs)are considered as one of the most promising candidates.However,the appli...The design and development of energy storage device with high energy/power density has become a research hotspot.Zinc-ion hybrid capacitors(ZHCs)are considered as one of the most promising candidates.However,the application of ZHCs is hindered by their low energy density at high power density due to the unsatisfactory cathode material.In this study,a novel 3D phosphorus-doped carbon nanotube/reduced graphene oxide(P-CNT/rGO)aerogel cathode is synthesized through a synergistic modification strategy of CNT insertion and P doping modification combined with 3D porous design.The as-obtained P-CNT/rGO aerogel cathode manifests significantly increased surface aera,expanded interlayer spacing,and enhanced pseudocapacitance behavior,thus leading to significantly enhanced specific capacitance and superb ions transport performance.The as-assembled ZHC based on P-CNT/rGO cathode delivers a superior energy density of 42.2 Wh/kg at an extreme-high power density of 80 kW/kg and excellent cycle life.In-depth kinetic analyses are undertaken to prove the enhanced pseudocapacitance behavior and exceptional power output capability of ZHCs.Furthermore,the reaction mechanism of physical and chemical adsorption/desorption of electrolyte ions on the P-CNT/rGO cathode is revealed by systematic ex-situ characterizations.This work can provide a valuable reference for developing advanced graphene-based cathode for high energy/power density ZHCs.展开更多
Small cell lung cancer(SCLC)is a phenotypically heterogeneous disease with an extremely poor prognosis,which is mainly attributed to the rapid development of resistance to chemotherapy.However,the relation between the...Small cell lung cancer(SCLC)is a phenotypically heterogeneous disease with an extremely poor prognosis,which is mainly attributed to the rapid development of resistance to chemotherapy.However,the relation between the growth phenotypes and chemo-resistance of SCLC remains largely unclear.Through comprehensive bioinformatic analyses,we found that the heterogeneity of SCLC phenotype was significantly associated with different sensitivity to chemotherapy.Adherent or semiadherent SCLC cells were enriched with activation of the PI3K/Akt/mTOR pathway and were highly chemoresistant.Mechanistically,activation of the PI3K/Akt/mTOR pathway promotes the phenotypic transition from suspension to adhesion growth pattern and confers SCLC cells with chemo-resistance.Such chemo-resistance could be largely overcome by combining chemotherapy with PI3K/Akt/mTOR pathway inhibitors.Our findings support that the PI3K/Akt/mTOR pathway plays an important role in SCLC phenotype transition and chemo-resistance,which holds important clinical implications for improving SCLC treatment.展开更多
In the References of the original publication[1]of this paper,the authors of ref.[26]should be'S.A.Bass,et al.(UrQMD Collaboration)',the authors of ref.[27]should be'M.Ble-icher,et al.(UrQMD Collaboration)...In the References of the original publication[1]of this paper,the authors of ref.[26]should be'S.A.Bass,et al.(UrQMD Collaboration)',the authors of ref.[27]should be'M.Ble-icher,et al.(UrQMD Collaboration)'.The authors of ref.[35]should be'W.Reisdorf,et al.(FOPI Collaboration)',展开更多
By considering different values of the time-step for the potential updates in the ultra-relativistic quantum molecular dynamics(UrQMD) model, we examine its influence on observables, such as the yield and collective f...By considering different values of the time-step for the potential updates in the ultra-relativistic quantum molecular dynamics(UrQMD) model, we examine its influence on observables, such as the yield and collective flow of nucleons and pions from heavyion collisions around 1 GeV/nucleon. It is found that these observables are affected to some extent by the choice of the time-step,and the impact of the time-step on the pion-related observables is more visible than that on the nucleon-related ones. However,its effect on the π-/π+yield ratio and elliptic flow difference between neutrons and protons, which have been taken as sensitive observables for probing the density-dependent nuclear symmetry energy at high densities, is fairly weak.展开更多
Red phosphorus(RP)is beneficial to industrialization due to its rich resources,chemical stability and environmental friendliness.However,the low electronic conductivity and large volume expansion limit its application...Red phosphorus(RP)is beneficial to industrialization due to its rich resources,chemical stability and environmental friendliness.However,the low electronic conductivity and large volume expansion limit its application for energy storage.Herein,we first used RP to prepare a novel bouquet-like Co_(3)(HPO_(4))_(2)(OH)_(2) by the hydrothermal approach as cathode materials for hybrid supercapacitors(HSC),which delivered a large specific capacity(119.2 mA h g^(−1) at 1 A g^(−1)),a superb rate capability(83.6 mA h g^(−1) at 100 A g^(−1))and a splendid electrochemical stability(92%capacity retention after 5000 cycles at 10 A g^(−1)).Furthermore,a novel HSC device assembled with Co_(3)(HPO_(4))_(2)(OH)_(2) as the cathode,porous carbon(PC)as the anode showed a high energy density of 44.6 W h kg^(−1) and a remarkable power density of 33.75 kW kg^(−1),along with an exceptional cyclic performance(91.8%capacity retention after 10,000 cycles at 3 A g^(−1)).This study not only develops a novel type of high-performance battery-type cathode material,but also provides a new idea for the industrial application of RP.展开更多
Small cell lung cancer(SCLC)is the most aggressive lung cancer with high heterogeneity.Mouse SCLC cells derived from the Rb1L/L/Trp53L/L(RP)autochthonous mouse model grew as adhesion or suspension in cell culture,and ...Small cell lung cancer(SCLC)is the most aggressive lung cancer with high heterogeneity.Mouse SCLC cells derived from the Rb1L/L/Trp53L/L(RP)autochthonous mouse model grew as adhesion or suspension in cell culture,and the adhesion cells are defined as non-neuroendocrine(non-NE)SCLC cells.Here,we uncover the heterogenous subpopulations within the non-NE cells and referred to them as mesenchymallike(Mes)and epithelial-like(Epi)SCLC cells.The Mes cells have increased capability to form colonies in soft agar and harbored stronger metastatic capability in vivo when compared with the Epi cells.Gene Set Enrichment Analysis reveals that the transforming growth factor(TGF)-βsignaling is enriched in the Mes cells.Importantly,inhibition of the TGF-βsignaling through ectopic expression of dominant-negative Tgfbr2(Tgfbr2-DN)or treatment with Tgfbr1 inhibitor SD-208 consistently abrogates tumor metastasis in nude mouse allograft assays.Moreover,genetic deletion of Tgfbr2 or Smad4,the key components of the TGF-βsignaling pathway,dramatically attenuates SCLC metastasis in the RP autochthonous mouse model.Collectively,our results uncover the high heterogeneity in non-NE SCLC cells and highlight an important role of TGF-βsignaling in promoting SCLC metastasis.展开更多
By modeling the fragmentation process using a dynamic model and permitting only evaporation in the statistical code,the main features of a projectile fragmentation at 600 MeV/u were considered in our previous study[Ph...By modeling the fragmentation process using a dynamic model and permitting only evaporation in the statistical code,the main features of a projectile fragmentation at 600 MeV/u were considered in our previous study[Phys.Rev.C,98:014610(2018)].In this study,we extend this to the isospin dependence of a projectile fragmentation at several hundreds of MeV/u.We searched for isospin observables related to the isospin fractionation to extract the symmetry energy,and found that at the pre-equilibrium stage of the collisions an isospin diffusion will take place and affect the isospin of the final fragments.The isospin fractionation plays a part during the fragmenting stage.Compared to the soft symmetry energy,the stiff symmetry energy provides a smaller repulsive force for neutrons and an attractive force for the protons in a neutron-rich system at a subnormal density,and hence causes a smaller isospin asymmetry of the gas phase,leaving a more neutron-rich liquid phase.An observable robust isospin is proposed to extract the slope of the symmetry energy at normal density based on the isospin dependence of the projectile fragmentation at hundreds of MeV/u.展开更多
基金Supported by the National Natural Science Foundation of China(No.31600035)to Shimei WUthe China Ocean Mineral Resources R&D Association Grant(No.DY135-B2-14)to Chaomin SUN。
文摘Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four diff erent categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.Their abilities to produce protease,amylase,and lipase were determined,and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions.The purifi cation of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate,and sequentially chromatographed by anion exchange column and gel filtration.The purifi ed protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE.The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80℃and a wide acidic range of pH 6.5-11.5,and displayed highest activity at 40℃ and pH 8.5.Notably,the protease still maintained high activity even at low to 10℃.Furthermore,the protease exhibited good stability in presence of diff erent surfactants,organic solvents,oxidizing agent H_(2)O_(2),and commercial detergents.Therefore,the protease produced by gcc-1 is a cold active and high stable enzyme,and has a promising potential in laundry detergent as an additive.
基金the financial support provided by the National Natural Science Foundation of China (Grant Nos. 22178056 & 22078060)the Natural Science Foundation of Fujian Province (Grant Nos. 2020J01555 & 2020J01881)+1 种基金the Opening Project of Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control (2019KF09)Special Fund for Science and Technology Innovation of Fujian Agriculture and Forestry University (Grant Nos. CXZX2019108S & CXZX2019116G)
文摘To fabricate an oil-water separation material that is rich in source,eco-friendly,and responsive,in this study,we successfully developed a collagen-based sponge for application to oil-water separation based on a green and facile strategy.In this design,widely-available collagen(COL)was used as the substrate:it was immersed in polydimethylsiloxane(PDMS)suspension with candle soot(CS)nanoparticles,followed by hot curing.The resultant sponge(CS/PDMS-COL)possessed good hydrophobicity with a water contact angle of 148.3°under a low PDMS concentration of 2%.The results from field emission scanning electron microscope,Fourier transform infrared spectrometer,X-ray photoelectron spectrometer,and X-ray diffractometry demonstrated the successful coating of CS and PDMS on the surface of COL substrate.The CS/PDMS-COL can adsorb eight oils,with the adsorption capacity for trichloromethane reaching 95 g/g.With benzene as the target adsorbent,the separation efficiency was maintained at no less than 95%even after recycling 20 times.CS/PDMS-COL was also used to separate oil-in-water emulsion.Moreover,the sponge killed bacteria effectively due to its excellent near-infrared photothermal responsiveness.This study provides new insight into the preparation of facile oil-water separation materials based on naturally occurring biomaterials effortlessly.
基金financially supported by Distinguished Young Scientists of Hunan Province(No.2022JJ10024)National Natural Science Foundation of China(No.21601057)+1 种基金Natural Science Foundation of Hunan Province(No.2021JJ30216)Key Projects of Hunan Provincial Education Department(No.22A0412).
文摘The design and development of energy storage device with high energy/power density has become a research hotspot.Zinc-ion hybrid capacitors(ZHCs)are considered as one of the most promising candidates.However,the application of ZHCs is hindered by their low energy density at high power density due to the unsatisfactory cathode material.In this study,a novel 3D phosphorus-doped carbon nanotube/reduced graphene oxide(P-CNT/rGO)aerogel cathode is synthesized through a synergistic modification strategy of CNT insertion and P doping modification combined with 3D porous design.The as-obtained P-CNT/rGO aerogel cathode manifests significantly increased surface aera,expanded interlayer spacing,and enhanced pseudocapacitance behavior,thus leading to significantly enhanced specific capacitance and superb ions transport performance.The as-assembled ZHC based on P-CNT/rGO cathode delivers a superior energy density of 42.2 Wh/kg at an extreme-high power density of 80 kW/kg and excellent cycle life.In-depth kinetic analyses are undertaken to prove the enhanced pseudocapacitance behavior and exceptional power output capability of ZHCs.Furthermore,the reaction mechanism of physical and chemical adsorption/desorption of electrolyte ions on the P-CNT/rGO cathode is revealed by systematic ex-situ characterizations.This work can provide a valuable reference for developing advanced graphene-based cathode for high energy/power density ZHCs.
基金supported by the National Natural Science Foundation of China(82030083 to H.J.,81871875 to L.H.)the National Basic Research Program of China(2017YFA0505501 to H.J.+8 种基金2020YFA0803300 to H.J.)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19020201 to H.J.)the National Natural Science Foundation of China(81872312 to H.J.,82011540007 to H.J.,31621003 to H.J.,81402371 to Y.J.,81802279 to H.H.,81902326 to X.W.,81602443 to X.L.)the Basic Frontier Scientific Research Program of Chinese Academy of Science(ZDBSLY-SM006 to H.J.)the International Cooperation Project of Chinese Academy of Sciences(153D31KYSB20190035 to H.J.)the Youth Innovation Promotion Association CAS(Y919S31371 to X.W.)the Natural Science Foundation of Hunan Province,China(2019JJ50550 to X.L.)Clinical Medical Technology Innovation Guide Project of Hunan(2020SK51827 to X.L.)Project of Scientific Research Plan of Hunan Provincial Health Commission(202103100127 to X.L.)。
文摘Small cell lung cancer(SCLC)is a phenotypically heterogeneous disease with an extremely poor prognosis,which is mainly attributed to the rapid development of resistance to chemotherapy.However,the relation between the growth phenotypes and chemo-resistance of SCLC remains largely unclear.Through comprehensive bioinformatic analyses,we found that the heterogeneity of SCLC phenotype was significantly associated with different sensitivity to chemotherapy.Adherent or semiadherent SCLC cells were enriched with activation of the PI3K/Akt/mTOR pathway and were highly chemoresistant.Mechanistically,activation of the PI3K/Akt/mTOR pathway promotes the phenotypic transition from suspension to adhesion growth pattern and confers SCLC cells with chemo-resistance.Such chemo-resistance could be largely overcome by combining chemotherapy with PI3K/Akt/mTOR pathway inhibitors.Our findings support that the PI3K/Akt/mTOR pathway plays an important role in SCLC phenotype transition and chemo-resistance,which holds important clinical implications for improving SCLC treatment.
文摘In the References of the original publication[1]of this paper,the authors of ref.[26]should be'S.A.Bass,et al.(UrQMD Collaboration)',the authors of ref.[27]should be'M.Ble-icher,et al.(UrQMD Collaboration)'.The authors of ref.[35]should be'W.Reisdorf,et al.(FOPI Collaboration)',
基金supported by the National Natural Science Foundation of China (Grant Nos. 11505057, 11547312, 11375062, and 11605270)the Department of Education of Zhejiang Province (Grant No. Y201533176)+1 种基金the China Postdoctoral Special Science Foundation (Grant No. 2016M591730)support by the computing server C3S2 in Huzhou University
文摘By considering different values of the time-step for the potential updates in the ultra-relativistic quantum molecular dynamics(UrQMD) model, we examine its influence on observables, such as the yield and collective flow of nucleons and pions from heavyion collisions around 1 GeV/nucleon. It is found that these observables are affected to some extent by the choice of the time-step,and the impact of the time-step on the pion-related observables is more visible than that on the nucleon-related ones. However,its effect on the π-/π+yield ratio and elliptic flow difference between neutrons and protons, which have been taken as sensitive observables for probing the density-dependent nuclear symmetry energy at high densities, is fairly weak.
基金financially supported by the National Natural Science Foundation of China(21601057)Hunan Provincial Natural Science Foundation(2021JJ30216)。
文摘Red phosphorus(RP)is beneficial to industrialization due to its rich resources,chemical stability and environmental friendliness.However,the low electronic conductivity and large volume expansion limit its application for energy storage.Herein,we first used RP to prepare a novel bouquet-like Co_(3)(HPO_(4))_(2)(OH)_(2) by the hydrothermal approach as cathode materials for hybrid supercapacitors(HSC),which delivered a large specific capacity(119.2 mA h g^(−1) at 1 A g^(−1)),a superb rate capability(83.6 mA h g^(−1) at 100 A g^(−1))and a splendid electrochemical stability(92%capacity retention after 5000 cycles at 10 A g^(−1)).Furthermore,a novel HSC device assembled with Co_(3)(HPO_(4))_(2)(OH)_(2) as the cathode,porous carbon(PC)as the anode showed a high energy density of 44.6 W h kg^(−1) and a remarkable power density of 33.75 kW kg^(−1),along with an exceptional cyclic performance(91.8%capacity retention after 10,000 cycles at 3 A g^(−1)).This study not only develops a novel type of high-performance battery-type cathode material,but also provides a new idea for the industrial application of RP.
基金supported by the National Natural Science Foundation of China(82030083 to H.J.,81871875 to L.H.)the National Basic Research Program of China(2017YFA0505501 to H.J.+4 种基金2020YFA0803300 to H.J.)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19020201 to H.J.)the National Natural Science Foundation of China(81872312 to H.J.,82011540007 to H.J.,31621003 to H.J.,81402371 to Y.J.)the Basic Frontier Scientific Research Program of Chinese Academy of Science(ZDBS-LY-SM006 to H.J.)the International Cooperation Project of Chinese Academy of Sciences(153D31KYSB20190035 to H.J.)。
文摘Small cell lung cancer(SCLC)is the most aggressive lung cancer with high heterogeneity.Mouse SCLC cells derived from the Rb1L/L/Trp53L/L(RP)autochthonous mouse model grew as adhesion or suspension in cell culture,and the adhesion cells are defined as non-neuroendocrine(non-NE)SCLC cells.Here,we uncover the heterogenous subpopulations within the non-NE cells and referred to them as mesenchymallike(Mes)and epithelial-like(Epi)SCLC cells.The Mes cells have increased capability to form colonies in soft agar and harbored stronger metastatic capability in vivo when compared with the Epi cells.Gene Set Enrichment Analysis reveals that the transforming growth factor(TGF)-βsignaling is enriched in the Mes cells.Importantly,inhibition of the TGF-βsignaling through ectopic expression of dominant-negative Tgfbr2(Tgfbr2-DN)or treatment with Tgfbr1 inhibitor SD-208 consistently abrogates tumor metastasis in nude mouse allograft assays.Moreover,genetic deletion of Tgfbr2 or Smad4,the key components of the TGF-βsignaling pathway,dramatically attenuates SCLC metastasis in the RP autochthonous mouse model.Collectively,our results uncover the high heterogeneity in non-NE SCLC cells and highlight an important role of TGF-βsignaling in promoting SCLC metastasis.
基金Supported by the National Natural Science Foundation of China(11875328,11605296,11635003)。
文摘By modeling the fragmentation process using a dynamic model and permitting only evaporation in the statistical code,the main features of a projectile fragmentation at 600 MeV/u were considered in our previous study[Phys.Rev.C,98:014610(2018)].In this study,we extend this to the isospin dependence of a projectile fragmentation at several hundreds of MeV/u.We searched for isospin observables related to the isospin fractionation to extract the symmetry energy,and found that at the pre-equilibrium stage of the collisions an isospin diffusion will take place and affect the isospin of the final fragments.The isospin fractionation plays a part during the fragmenting stage.Compared to the soft symmetry energy,the stiff symmetry energy provides a smaller repulsive force for neutrons and an attractive force for the protons in a neutron-rich system at a subnormal density,and hence causes a smaller isospin asymmetry of the gas phase,leaving a more neutron-rich liquid phase.An observable robust isospin is proposed to extract the slope of the symmetry energy at normal density based on the isospin dependence of the projectile fragmentation at hundreds of MeV/u.
