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Involvement of cysteinyl leukotriene signaling in microglial morphological changes and CASP1 expression in vitro
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作者 Yu-xi WANG Si-ran ZHANG +5 位作者 Xue XU cheng-tan li Wei ZHENG Jian-bo ZHAO Yan-fang WANG li-hui ZHANG 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2017年第10期1028-1029,共2页
OBJECTIVE We have recently reported that cysteinyl leukotriene(Cys LT) signaling plays an important role in microglial interleukin(IL)-1β secretion and subsequent neurotoxicity.The present study aimed to examine micr... OBJECTIVE We have recently reported that cysteinyl leukotriene(Cys LT) signaling plays an important role in microglial interleukin(IL)-1β secretion and subsequent neurotoxicity.The present study aimed to examine microglial morphological changes and the upstream molecular underlying IL^(-1)β production in Cys LT receptor agonist leukotriene D4(LTD4)-treated BV2 microglia in vitro.METHODS Twenty-four hours after murine microglial BV2 cells were stimulated with LTD4(1-100 nmol·L^(-1)),the cell proliferation and morphology were observed.The expression level of cysteinyl aspartate-specific protease 1(CASP1) protein was measured by Western blotin BV2 cells.In addition,BV2 cells were pretreated with or without CysLT1 receptor antagonist montelukast for 1 h and the effects of monte-lukaston LTD4-stimulated microglial activation and CASP1 expression were evaluated.RESULTS The number of BV2 cells had an increasing tendency after 24 h treatment with LTD4,but no significant differences were observed between the control and LTD4-treated cells(P>0.05).Under basal and resting conditions,BV2 microglial cells displayed a ramified morphology.However,LTD4 at 100 nmool·L^(-1) drove microglial morphological changes from a ramified towards an amoeboid shape.The expression of CASP1 protein was significantly upregulated in 100 nmool·L^(-1) LTD4-treated BV2 microglia(P<0.01).Furthermore,pretreatment with CysLT1 receptor antagonist montelukast prevented cell morphological changes and suppressed the increased CASP1 expression in LTD4-treated BV2 cells(P<0.05).CONCLUSION Cys LT receptor agonist LTD4 induces morphological changes and CASP1 expressionin BV2 microglia,which can be inhibited by CysLT1 antagonist.These results suggest the involvement of Cys LT signaling in microglial morphological changes and CASP1 expression. 展开更多
关键词 cysteinyl leukotriene RECEPTOR MICROGLIA INFLAMMATION
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Oxygen-Glucose Deprivation Activates 5-Lipoxygenase Mediated by Oxidant Stress through p38 MAPK Pathway in PC12 cells
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作者 cheng-tan li Wei-Ping Zhang +8 位作者 Yun-Bi Lu San-Hua Fang Yu-Mei Yuan ling-ling Qi li-Hui Zhang Xiao-Jia Huang Lei Zhang Zhong Chen Er-Qing Wei 《中国药理通讯》 2008年第3期30-31,共2页
关键词 葡萄糖 PC12细胞 氧化剂
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