Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on...Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on the growthof tartary buckwheat seedling roots, and the alleviation of Al stress by silicon (Si), as has been demonstrated inmany crops. Under Al stress, root growth (total root length, primary root length, root tips, root surface area, androot volume) was significantly inhibited, and Al and malondialdehyde (MDA) accumulated in the root tips. At thesame time, catalase (CAT) and ascorbate peroxidase activities, polyphenols, flavonoids, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free-radical scavenging abilitywere significantly decreased. After the application of Si, root growth, Al accumulation, and oxidative damage wereimproved. Compared to Al-treated seedlings, the contents of ·O2− and MDA decreased by 29.39% and 25.22%,respectively. This was associated with Si-induced increases in peroxidase and CAT enzyme activity, flavonoidcompounds, and free-radical scavenging (DPPH and ABTS). The application of Si therefore has positive effectson Al toxicity in tartary buckwheat roots by reducing Al accumulation in the roots and maintaining oxidationhomeostasis.展开更多
[Objectives] Rice-Tartary buckwheat is a special type of Tartary buckwheat with easy hulling thin shell.This study was conducted to characterize the carbohydrate metabolism,quality and yield and provide a basis for fo...[Objectives] Rice-Tartary buckwheat is a special type of Tartary buckwheat with easy hulling thin shell.This study was conducted to characterize the carbohydrate metabolism,quality and yield and provide a basis for food processing and breeding of rice-Tartary buckwheat.[Methods]The local rice-Tartary buckwheat‘cv.Xiaomiqiao’(XMQ) was used to explore the characteristics of photosynthesis,starch synthesis enzyme activities,traits of yield and quality,as compared to Tartary buckwheat‘cv.Jinqiao 2’(JQ2).[Results]XMQ showed significantly lower values of net photosynthetic rate,ADPGase activity and starch synthase activity at one or more periods during grain filling than JQ2.The effective branch number and grain number per plant of XMQ were similar to that of JQ2 regardless of different years,but the 1 000-grain weight was significantly lighter that resulted in the significantly lower yield.XMQ accumulated similar contents of starch(amylose,amylopectin) and protein(glutelin,albumin,prolamin,globulin) to those of JQ2,but exhibited significantly lower flavonoid content.The values of peak viscosity and hot paste viscosity in XMQ were similar to those of JQ2,but the values of cool paste viscosity,breakdown and setback were significantly lower.In conclusion,XMQ showed low yield due to the insufficient of photosynthesis and starch synthesis.XMQ exhibited high nutritional quality as well as normal Tartary buckwheat and stored 18.88 mg/g of flavonoid,that might be consumed as daily diet.[Conclusions]The present results will provide a basis for utilization and improvement of rice-Tartary buckwheat.展开更多
Rice-Tartary buckwheat(RTB)is a special germplasm of Tartary buckwheat.In this study,the appraisal of taste quality between RTB and conventional Tartary buckwheat(CTB)was presented,and the metabolites in kernels at th...Rice-Tartary buckwheat(RTB)is a special germplasm of Tartary buckwheat.In this study,the appraisal of taste quality between RTB and conventional Tartary buckwheat(CTB)was presented,and the metabolites in kernels at three typical grain filling stage(GFS)were investigated.Unlike CTB,RTB showed thin shell seeds without longitudinal furrows at maturity,which was easily artificially dehulled.Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance,viscosity,taste and summary were appraised.In total,92 metabolites were identified in kernels using GM-MS metabolomics platform.The levels of most metabolites changed greatly during grain filling and a large numbers of metabolite-metabolite correlations were found by Pearson correlation coefficient analysis.ANOVA analysis identified 61 differentially expressed metabolites between RTB and CTB,while Venn diagram analysis screened 35 common differential metabolites.Compared with CTB,RTB showed similar levels of lysine and methionine,indicated that RTB own excellent nutritional value.Additionally,RTB exhibited significantly up-regulated levels of most sweeteners(sugars and polyols),which might contribute to better taste.This work provides the first comprehensive metabolomics analysis of kernels between RTB and CTB,which may potentially provide theoretical basis for further research.展开更多
A low seed-setting rate is the main limiting factor influencing Tartary buckwheat production under high-yield cultivation conditions.To investigate the seed setting and its spatial characteristics,two Tartary buckwhea...A low seed-setting rate is the main limiting factor influencing Tartary buckwheat production under high-yield cultivation conditions.To investigate the seed setting and its spatial characteristics,two Tartary buckwheat cultivars(high seed-setting rate cultivar Qianku 3;low seed-setting rate cultivar Liuku 3)were compared by a two-year field trial in 2017 and 2018.The results showed that the Tartary buckwheat underwent simultaneous flowering and fruiting.Flowers,generated from branch,were still blooming during the mature stage of grains on stem,which resulting in a greater number of flowers and grains on the branch than those on the stem at the low part of plant.The seed-setting characteristics significantly differed between two cultivars.The high seed-setting rate of Qianku 3 was 26.6%and 33.2%higher than Liuku 3 in 2017 and 2018,respectively.Meanwhile,Qianku 3 showed a higher filled grain number(157.8%)and seed-setting rate(66.4%)on branch than Liuku 3.A network analysis showed that the whole-plant seed-setting rate was positively correlated with grain number,which was closely correlated with flower number at the same position of plant.The path-coefficient analysis revealed that grains number on branches was the most dominant component(Path coefficient(P)=2.19)of the seed-setting rate,followed by grains number on stem(P=0.60).The grains number on branches showed the greatest positive direct effect with significant correlation(r=0.76 and P<0.01)on the seed-setting rate.Overall,the present study indicated that the grain number of branches may play a vital role in improving the seed-setting rate in Tartary buckwheat.展开更多
In this paper,a new 3D visualization technical method was developed for hydraulic fractures using micro-seismic monitoring.This technical method consists of four steps:i.interpret the geologic hydraulic fracture model...In this paper,a new 3D visualization technical method was developed for hydraulic fractures using micro-seismic monitoring.This technical method consists of four steps:i.interpret the geologic hydraulic fracture model based on seismic source location data from micro-seismic monitoring;ii.develop a hydraulic fracture indication model,relying on the 3D spatial freeze-frame of micro-seismic monitoring sources from hydraulic fracturing;iii.construct a hydraulic fracture density model using the intensity from the micro-seismic monitoring;and iv.implement a 3D visualization of the hydraulic fractures,relying on the spatial constraints of the density model,the hydraulic fracture indication model,and the properties of the hydraulic fractures.This proposed technical method was used to produce 3D visualizations of the hydraulic fractures in well X in the Jiao reservoir,China,and the 3D visualizations of the distribution,development,extent and cutting relationships of hydraulic fractures were successfully realized.The results show that this technical method can be used as a practical and reliable approach to characterize hydraulic fractures.展开更多
基金Science&Technology Department of Sichuan Province(2022YFQ0041,2022NSFSC1725,2023NSFSC0214)China Agriculture Research System(CARS-07-B-1)+2 种基金The National Natural Science Foundation of China(32160428)Innovative Training Program for College Students(202311079040,S202311079112,CDUCX2023550)Undergraduate Education and Teaching Reform Project of Chengdu University(cdjgb2022186).
文摘Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on the growthof tartary buckwheat seedling roots, and the alleviation of Al stress by silicon (Si), as has been demonstrated inmany crops. Under Al stress, root growth (total root length, primary root length, root tips, root surface area, androot volume) was significantly inhibited, and Al and malondialdehyde (MDA) accumulated in the root tips. At thesame time, catalase (CAT) and ascorbate peroxidase activities, polyphenols, flavonoids, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free-radical scavenging abilitywere significantly decreased. After the application of Si, root growth, Al accumulation, and oxidative damage wereimproved. Compared to Al-treated seedlings, the contents of ·O2− and MDA decreased by 29.39% and 25.22%,respectively. This was associated with Si-induced increases in peroxidase and CAT enzyme activity, flavonoidcompounds, and free-radical scavenging (DPPH and ABTS). The application of Si therefore has positive effectson Al toxicity in tartary buckwheat roots by reducing Al accumulation in the roots and maintaining oxidationhomeostasis.
基金Supported by National Natural Science Foundation of China(31660366)Natural Science Foundation of Guizhou Province(QKH CG[2019]4336,QKH ZC[2019]2298,QKH LH[2017]7359,QKH PT[2017]5726)。
文摘[Objectives] Rice-Tartary buckwheat is a special type of Tartary buckwheat with easy hulling thin shell.This study was conducted to characterize the carbohydrate metabolism,quality and yield and provide a basis for food processing and breeding of rice-Tartary buckwheat.[Methods]The local rice-Tartary buckwheat‘cv.Xiaomiqiao’(XMQ) was used to explore the characteristics of photosynthesis,starch synthesis enzyme activities,traits of yield and quality,as compared to Tartary buckwheat‘cv.Jinqiao 2’(JQ2).[Results]XMQ showed significantly lower values of net photosynthetic rate,ADPGase activity and starch synthase activity at one or more periods during grain filling than JQ2.The effective branch number and grain number per plant of XMQ were similar to that of JQ2 regardless of different years,but the 1 000-grain weight was significantly lighter that resulted in the significantly lower yield.XMQ accumulated similar contents of starch(amylose,amylopectin) and protein(glutelin,albumin,prolamin,globulin) to those of JQ2,but exhibited significantly lower flavonoid content.The values of peak viscosity and hot paste viscosity in XMQ were similar to those of JQ2,but the values of cool paste viscosity,breakdown and setback were significantly lower.In conclusion,XMQ showed low yield due to the insufficient of photosynthesis and starch synthesis.XMQ exhibited high nutritional quality as well as normal Tartary buckwheat and stored 18.88 mg/g of flavonoid,that might be consumed as daily diet.[Conclusions]The present results will provide a basis for utilization and improvement of rice-Tartary buckwheat.
基金This research was funded in part by the National Natural Science Foundation of China(Grant Nos.31660366,31660424)Natural Science Foundation of Guizhou Province(QKH ZC[2019]2298,QKH CG[2019]4336)+1 种基金the construction of the Key Laboratory for Conservation and Innovation of Buckwheat Germplasm in Guizhou(QJH KY Zi[2017]002)Doctor Research Fund in Guizhou Normal University.
文摘Rice-Tartary buckwheat(RTB)is a special germplasm of Tartary buckwheat.In this study,the appraisal of taste quality between RTB and conventional Tartary buckwheat(CTB)was presented,and the metabolites in kernels at three typical grain filling stage(GFS)were investigated.Unlike CTB,RTB showed thin shell seeds without longitudinal furrows at maturity,which was easily artificially dehulled.Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance,viscosity,taste and summary were appraised.In total,92 metabolites were identified in kernels using GM-MS metabolomics platform.The levels of most metabolites changed greatly during grain filling and a large numbers of metabolite-metabolite correlations were found by Pearson correlation coefficient analysis.ANOVA analysis identified 61 differentially expressed metabolites between RTB and CTB,while Venn diagram analysis screened 35 common differential metabolites.Compared with CTB,RTB showed similar levels of lysine and methionine,indicated that RTB own excellent nutritional value.Additionally,RTB exhibited significantly up-regulated levels of most sweeteners(sugars and polyols),which might contribute to better taste.This work provides the first comprehensive metabolomics analysis of kernels between RTB and CTB,which may potentially provide theoretical basis for further research.
基金the National Natural Science Foundation of China(31771716)China Agriculture Research System(CARS-07-B-1)to facilitate the research.
文摘A low seed-setting rate is the main limiting factor influencing Tartary buckwheat production under high-yield cultivation conditions.To investigate the seed setting and its spatial characteristics,two Tartary buckwheat cultivars(high seed-setting rate cultivar Qianku 3;low seed-setting rate cultivar Liuku 3)were compared by a two-year field trial in 2017 and 2018.The results showed that the Tartary buckwheat underwent simultaneous flowering and fruiting.Flowers,generated from branch,were still blooming during the mature stage of grains on stem,which resulting in a greater number of flowers and grains on the branch than those on the stem at the low part of plant.The seed-setting characteristics significantly differed between two cultivars.The high seed-setting rate of Qianku 3 was 26.6%and 33.2%higher than Liuku 3 in 2017 and 2018,respectively.Meanwhile,Qianku 3 showed a higher filled grain number(157.8%)and seed-setting rate(66.4%)on branch than Liuku 3.A network analysis showed that the whole-plant seed-setting rate was positively correlated with grain number,which was closely correlated with flower number at the same position of plant.The path-coefficient analysis revealed that grains number on branches was the most dominant component(Path coefficient(P)=2.19)of the seed-setting rate,followed by grains number on stem(P=0.60).The grains number on branches showed the greatest positive direct effect with significant correlation(r=0.76 and P<0.01)on the seed-setting rate.Overall,the present study indicated that the grain number of branches may play a vital role in improving the seed-setting rate in Tartary buckwheat.
基金We are grateful for the support of the National Major Basic Research Development Program of China(2014CB239205)the Sichuan Science and Technology Program(Grant No.2021YFQ0049)the sub-project of the National Science and Technology Major Project(Grant No.2017ZX05035003).
文摘In this paper,a new 3D visualization technical method was developed for hydraulic fractures using micro-seismic monitoring.This technical method consists of four steps:i.interpret the geologic hydraulic fracture model based on seismic source location data from micro-seismic monitoring;ii.develop a hydraulic fracture indication model,relying on the 3D spatial freeze-frame of micro-seismic monitoring sources from hydraulic fracturing;iii.construct a hydraulic fracture density model using the intensity from the micro-seismic monitoring;and iv.implement a 3D visualization of the hydraulic fractures,relying on the spatial constraints of the density model,the hydraulic fracture indication model,and the properties of the hydraulic fractures.This proposed technical method was used to produce 3D visualizations of the hydraulic fractures in well X in the Jiao reservoir,China,and the 3D visualizations of the distribution,development,extent and cutting relationships of hydraulic fractures were successfully realized.The results show that this technical method can be used as a practical and reliable approach to characterize hydraulic fractures.
