Vascular etiology is the second most prevalent cause of cognitive impairment globally.Endothelin-1,which is produced and secreted by endothelial cells and astrocytes,is implicated in the pathogenesis of stroke.However...Vascular etiology is the second most prevalent cause of cognitive impairment globally.Endothelin-1,which is produced and secreted by endothelial cells and astrocytes,is implicated in the pathogenesis of stroke.However,the way in which changes in astrocytic endothelin-1 lead to poststroke cognitive deficits following transient middle cerebral artery occlusion is not well understood.Here,using mice in which astrocytic endothelin-1 was overexpressed,we found that the selective overexpression of endothelin-1 by astrocytic cells led to ischemic stroke-related dementia(1 hour of ischemia;7 days,28 days,or 3 months of reperfusion).We also revealed that astrocytic endothelin-1 overexpression contributed to the role of neural stem cell proliferation but impaired neurogenesis in the dentate gyrus of the hippocampus after middle cerebral artery occlusion.Comprehensive proteome profiles and western blot analysis confirmed that levels of glial fibrillary acidic protein and peroxiredoxin 6,which were differentially expressed in the brain,were significantly increased in mice with astrocytic endothelin-1 overexpression in comparison with wild-type mice 28 days after ischemic stroke.Moreover,the levels of the enriched differentially expressed proteins were closely related to lipid metabolism,as indicated by Kyoto Encyclopedia of Genes and Genomes pathway analysis.Liquid chromatography-mass spectrometry nontargeted metabolite profiling of brain tissues showed that astrocytic endothelin-1 overexpression altered lipid metabolism products such as glycerol phosphatidylcholine,sphingomyelin,and phosphatidic acid.Overall,this study demonstrates that astrocytic endothelin-1 overexpression can impair hippocampal neurogenesis and that it is correlated with lipid metabolism in poststroke cognitive dysfunction.展开更多
Graphene,as a proof-of-concept two-dimensional material,has proven to have excellent physical and chemical properties.Its derivatives,such as defective or doped graphene,are also applied as catalytic materials for met...Graphene,as a proof-of-concept two-dimensional material,has proven to have excellent physical and chemical properties.Its derivatives,such as defective or doped graphene,are also applied as catalytic materials for metal-air batteries(MABs).MABs have been recognized as possible candidates for new-generation energy storage systems due to their ultra-high theoretical energy density.So far,graphene and its derivatives with optimized structures have been widely explored to improve the electrochemical performance in MABs.Generally speaking,perfect graphene crystalline is inert for many catalytic processes,while defects and heteroatoms can endow graphene with high activity for many electrocatalytic reactions.Under this circumstance,recent progress is summarized for defective/doped graphene as air cathodes in aqueous or organic MABs,which are actually different electrochemical systems with distinct requirements for air cathodes.Also,the relationship is clarified between graphene defects/doping and electrocatalytic mechanisms that can be the guidance for catalyst design.Future directions are also prospected for the development of graphene-based MAB cathodes.展开更多
This paper briefly analyzes the advantages of IPv6 used at Intelligent home and describes an IPv6 home network frame. It also studies IPv6 transition technical problems facing home gateways and gives the summaries to ...This paper briefly analyzes the advantages of IPv6 used at Intelligent home and describes an IPv6 home network frame. It also studies IPv6 transition technical problems facing home gateways and gives the summaries to the main protocol of each node of home network and their mutual relations. The key nodes of the home network based on IPv6, including the hardware design and software design of the home gateway and home network controller, are systematically de-signed in the paper. Finally, it illustrates the actual test environment and test in detail. The paper gives a view of the fact that the intelligent home appliances are becoming the current trend with the accelerating the transition of IPv4 to IPv6 and home network-wide implementation of IPv6 is about to become a reality, holding that the network controllers and home gateway based on ARM processor can support IPv6 and carry on the implementation of remote control and local control based on Web Browser.展开更多
A lot of technologies can be used in home control subnet, but the hardware and software resources available for the home control subnet are limited. There are security problems easily seen. The paper gives the system-...A lot of technologies can be used in home control subnet, but the hardware and software resources available for the home control subnet are limited. There are security problems easily seen. The paper gives the system-atic analysis of the structure and function of home control subnet based on the general model of home net-work. The paper has also analyzed two types of major equipment, namely sub-gateways and terminal equip-ment. The major networking technology used in home control subnet is summarized and concluded. In com-bination with relationship among home control subnet, home network, as well as the outside main network, the paper has systematically studied various safety problems related to home control gateways and the possi-ble solutions to those problems have been made.展开更多
The recent fast development in computer vision and mobile sensor technology such as mobile LiDAR and RGB-D cameras is pushing the boundary of the technology to suit the need of real-life applications in the fields of ...The recent fast development in computer vision and mobile sensor technology such as mobile LiDAR and RGB-D cameras is pushing the boundary of the technology to suit the need of real-life applications in the fields of Augmented Reality(AR),robotics,indoor GIS and self-driving.Camera localization is often a key and enabling technology among these applications.In this paper,we developed a novel camera localization workflow based on a highly accurate 3D prior map optimized by our RGBD SLAM method in conjunction with a deep learning routine trained using consecutive video frames labeled with high precision camera pose.Furthermore,an AR registration method tightly coupled with a game engine is proposed,which incorporates the proposed localization algorithm and aligns the real Kinetic camera with a virtual camera of the game engine to facilitate AR application development in an integrated manner.The experimental results show that the localization accuracy can achieve an average error of 35 cm based on a fine-tuned prior 3D feature database at 3 cm accuracy compared against the ground-truth 3D LiDAR map.The influence of the localization accuracy on the visual effect of AR overlay is also demonstrated and the alignment of the real and virtual camera streamlines the implementation of AR fire emergency response demo in a Virtual Geographic Environment.展开更多
The adsorption and desorption behavior of Cr(Ⅵ) in membrane capacitive deionization(MCDI) was investigated systematically in the presence of bovine serum albumin(BSA) and KCl with different concentrations, respective...The adsorption and desorption behavior of Cr(Ⅵ) in membrane capacitive deionization(MCDI) was investigated systematically in the presence of bovine serum albumin(BSA) and KCl with different concentrations, respectively. Results revealed that Cr(Ⅵ) absorption was enhanced and the adsorption amount for Cr(Ⅵ) increased from 155.7 to 190.8 mg/g when KCl concentration increased from 100 to 200 mg/L in the adsorption process, which was attributed to the stronger driving force. However, the adsorption amount sharply decreased to 90.2 mg/g when KCl concentration reached up to 1000 mg/L suggesting the negative effect for Cr(Ⅵ) removal that high KCl concentration had. As for the effect of BSA on ion adsorption, the amount for Cr(Ⅵ) significantly declined to 78.3 mg/g and p H was found to be an important factor contributing to this significant reduction. Then, the desorption performance was also conducted and it was obtained that the presence of KCl had negligible effect on Cr(Ⅵ) desorption, while promoted by the addition of BSA. The incomplete desorption was obtained and the residual chromium ions onto the electrode after desorption was detected via energy-dispersive X-ray spectroscopy(EDS). Based on above analysis, the enhanced removal mechanism for Cr(Ⅵ) in MCDI was found to be consisted of ion adsorption onto electrode surface, the redox reaction of Cr(Ⅵ) into Cr(III)and precipitation, which was demonstrated by X-ray photoelectron spectroscopy(XPS) and scanning electron microscope(SEM).展开更多
The preparation of enantiomerically pure 1,4-oxazines remains a continuous challenge in synthetic chemistry because of their potential application in the total synthesis of morpholines.Herein,a one-pot asymmetric tran...The preparation of enantiomerically pure 1,4-oxazines remains a continuous challenge in synthetic chemistry because of their potential application in the total synthesis of morpholines.Herein,a one-pot asymmetric transfer hydrogenation/cyclization enantio-relay process catalyzed by Ru and Au bimetallic catalysts was developed.This enantio-relay process firstly allows the asymmetric transfer hydrogenation of alkynones to form chiral alkynols in the presence of(S,S)-mesityleneRuClTsDPEN(TsDPEN=N-(p-toluenesulfonyl)-1,2-diphenylethylenediamine)and then the chiral alkynols could be converted into 3,4-dihydro-2H-1,4-oxazines in moderate to good yields with excellent enantioselectivity retention accompanied by AuCl(PPh_(3))/AgNTf_(2) and HCOOH.A series of sulfonamide-tethered alkynones were well tolerated in this process.Mechanistic studies indicated that the alkynols formed in the first step were initially transformed into a methylenemorpholine intermediate when AuCl(PPh_(3))/AgNTf_(2) and HCOOH were added,which can be further isomerized into chiral 3,4-dihydro-2H-1,4-oxazines with the addition of a second HCOOH.This protocol offers not only a practical method to access 3,4-dihydro-2H-1,4-oxazines from alkynones in an enantioselective version,but also enriches the arsenal of gold chemistry from a mechanistic point of view.展开更多
基金financially supported by the National Natural Science Foundation of China,No.81303115,81774042 (both to XC)the Pearl River S&T Nova Program of Guangzhou,No.201806010025 (to XC)+3 种基金the Specialty Program of Guangdong Province Hospital of Chinese Medicine of China,No.YN2018ZD07 (to XC)the Natural Science Foundatior of Guangdong Province of China,No.2023A1515012174 (to JL)the Science and Technology Program of Guangzhou of China,No.20210201 0268 (to XC),20210201 0339 (to JS)Guangdong Provincial Key Laboratory of Research on Emergency in TCM,Nos.2018-75,2019-140 (to JS)
文摘Vascular etiology is the second most prevalent cause of cognitive impairment globally.Endothelin-1,which is produced and secreted by endothelial cells and astrocytes,is implicated in the pathogenesis of stroke.However,the way in which changes in astrocytic endothelin-1 lead to poststroke cognitive deficits following transient middle cerebral artery occlusion is not well understood.Here,using mice in which astrocytic endothelin-1 was overexpressed,we found that the selective overexpression of endothelin-1 by astrocytic cells led to ischemic stroke-related dementia(1 hour of ischemia;7 days,28 days,or 3 months of reperfusion).We also revealed that astrocytic endothelin-1 overexpression contributed to the role of neural stem cell proliferation but impaired neurogenesis in the dentate gyrus of the hippocampus after middle cerebral artery occlusion.Comprehensive proteome profiles and western blot analysis confirmed that levels of glial fibrillary acidic protein and peroxiredoxin 6,which were differentially expressed in the brain,were significantly increased in mice with astrocytic endothelin-1 overexpression in comparison with wild-type mice 28 days after ischemic stroke.Moreover,the levels of the enriched differentially expressed proteins were closely related to lipid metabolism,as indicated by Kyoto Encyclopedia of Genes and Genomes pathway analysis.Liquid chromatography-mass spectrometry nontargeted metabolite profiling of brain tissues showed that astrocytic endothelin-1 overexpression altered lipid metabolism products such as glycerol phosphatidylcholine,sphingomyelin,and phosphatidic acid.Overall,this study demonstrates that astrocytic endothelin-1 overexpression can impair hippocampal neurogenesis and that it is correlated with lipid metabolism in poststroke cognitive dysfunction.
文摘Graphene,as a proof-of-concept two-dimensional material,has proven to have excellent physical and chemical properties.Its derivatives,such as defective or doped graphene,are also applied as catalytic materials for metal-air batteries(MABs).MABs have been recognized as possible candidates for new-generation energy storage systems due to their ultra-high theoretical energy density.So far,graphene and its derivatives with optimized structures have been widely explored to improve the electrochemical performance in MABs.Generally speaking,perfect graphene crystalline is inert for many catalytic processes,while defects and heteroatoms can endow graphene with high activity for many electrocatalytic reactions.Under this circumstance,recent progress is summarized for defective/doped graphene as air cathodes in aqueous or organic MABs,which are actually different electrochemical systems with distinct requirements for air cathodes.Also,the relationship is clarified between graphene defects/doping and electrocatalytic mechanisms that can be the guidance for catalyst design.Future directions are also prospected for the development of graphene-based MAB cathodes.
文摘This paper briefly analyzes the advantages of IPv6 used at Intelligent home and describes an IPv6 home network frame. It also studies IPv6 transition technical problems facing home gateways and gives the summaries to the main protocol of each node of home network and their mutual relations. The key nodes of the home network based on IPv6, including the hardware design and software design of the home gateway and home network controller, are systematically de-signed in the paper. Finally, it illustrates the actual test environment and test in detail. The paper gives a view of the fact that the intelligent home appliances are becoming the current trend with the accelerating the transition of IPv4 to IPv6 and home network-wide implementation of IPv6 is about to become a reality, holding that the network controllers and home gateway based on ARM processor can support IPv6 and carry on the implementation of remote control and local control based on Web Browser.
文摘A lot of technologies can be used in home control subnet, but the hardware and software resources available for the home control subnet are limited. There are security problems easily seen. The paper gives the system-atic analysis of the structure and function of home control subnet based on the general model of home net-work. The paper has also analyzed two types of major equipment, namely sub-gateways and terminal equip-ment. The major networking technology used in home control subnet is summarized and concluded. In com-bination with relationship among home control subnet, home network, as well as the outside main network, the paper has systematically studied various safety problems related to home control gateways and the possi-ble solutions to those problems have been made.
基金This work was funded by the National Key Research and Development Program of China[grant number 2016YFB0502102]It was also partially funded by National Natural Science Foundation of China[grant number 41101436]the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry。
文摘The recent fast development in computer vision and mobile sensor technology such as mobile LiDAR and RGB-D cameras is pushing the boundary of the technology to suit the need of real-life applications in the fields of Augmented Reality(AR),robotics,indoor GIS and self-driving.Camera localization is often a key and enabling technology among these applications.In this paper,we developed a novel camera localization workflow based on a highly accurate 3D prior map optimized by our RGBD SLAM method in conjunction with a deep learning routine trained using consecutive video frames labeled with high precision camera pose.Furthermore,an AR registration method tightly coupled with a game engine is proposed,which incorporates the proposed localization algorithm and aligns the real Kinetic camera with a virtual camera of the game engine to facilitate AR application development in an integrated manner.The experimental results show that the localization accuracy can achieve an average error of 35 cm based on a fine-tuned prior 3D feature database at 3 cm accuracy compared against the ground-truth 3D LiDAR map.The influence of the localization accuracy on the visual effect of AR overlay is also demonstrated and the alignment of the real and virtual camera streamlines the implementation of AR fire emergency response demo in a Virtual Geographic Environment.
基金financially supported by the National Natural Science Fund of China (No. 51508153)the Natural Science Fund of Jiangsu (No. BK20150813)+1 种基金the Fundamental Research Funds for the Central UniversitiesA Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The adsorption and desorption behavior of Cr(Ⅵ) in membrane capacitive deionization(MCDI) was investigated systematically in the presence of bovine serum albumin(BSA) and KCl with different concentrations, respectively. Results revealed that Cr(Ⅵ) absorption was enhanced and the adsorption amount for Cr(Ⅵ) increased from 155.7 to 190.8 mg/g when KCl concentration increased from 100 to 200 mg/L in the adsorption process, which was attributed to the stronger driving force. However, the adsorption amount sharply decreased to 90.2 mg/g when KCl concentration reached up to 1000 mg/L suggesting the negative effect for Cr(Ⅵ) removal that high KCl concentration had. As for the effect of BSA on ion adsorption, the amount for Cr(Ⅵ) significantly declined to 78.3 mg/g and p H was found to be an important factor contributing to this significant reduction. Then, the desorption performance was also conducted and it was obtained that the presence of KCl had negligible effect on Cr(Ⅵ) desorption, while promoted by the addition of BSA. The incomplete desorption was obtained and the residual chromium ions onto the electrode after desorption was detected via energy-dispersive X-ray spectroscopy(EDS). Based on above analysis, the enhanced removal mechanism for Cr(Ⅵ) in MCDI was found to be consisted of ion adsorption onto electrode surface, the redox reaction of Cr(Ⅵ) into Cr(III)and precipitation, which was demonstrated by X-ray photoelectron spectroscopy(XPS) and scanning electron microscope(SEM).
基金the National Natural Science Foundation of China(22001170,22071154 and 21872095)the Shanghai Sciences and Technologies Development Fund(20070502600)the Shanghai Frontiers Science Research Base of Biomimetic Catalysis for financial support.
文摘The preparation of enantiomerically pure 1,4-oxazines remains a continuous challenge in synthetic chemistry because of their potential application in the total synthesis of morpholines.Herein,a one-pot asymmetric transfer hydrogenation/cyclization enantio-relay process catalyzed by Ru and Au bimetallic catalysts was developed.This enantio-relay process firstly allows the asymmetric transfer hydrogenation of alkynones to form chiral alkynols in the presence of(S,S)-mesityleneRuClTsDPEN(TsDPEN=N-(p-toluenesulfonyl)-1,2-diphenylethylenediamine)and then the chiral alkynols could be converted into 3,4-dihydro-2H-1,4-oxazines in moderate to good yields with excellent enantioselectivity retention accompanied by AuCl(PPh_(3))/AgNTf_(2) and HCOOH.A series of sulfonamide-tethered alkynones were well tolerated in this process.Mechanistic studies indicated that the alkynols formed in the first step were initially transformed into a methylenemorpholine intermediate when AuCl(PPh_(3))/AgNTf_(2) and HCOOH were added,which can be further isomerized into chiral 3,4-dihydro-2H-1,4-oxazines with the addition of a second HCOOH.This protocol offers not only a practical method to access 3,4-dihydro-2H-1,4-oxazines from alkynones in an enantioselective version,but also enriches the arsenal of gold chemistry from a mechanistic point of view.
