β-Cyclodextrin （β-CD） and its derivatives functionalized magnetic nanoparticles （MNPs） with high saturated magnetism were fabricated successfully by an effective grafting method. The resultant carboxymethyl/hydr...β-Cyclodextrin （β-CD） and its derivatives functionalized magnetic nanoparticles （MNPs） with high saturated magnetism were fabricated successfully by an effective grafting method. The resultant carboxymethyl/hydroxy- propyl/sulfobutyl ether-β-CD-MNPs （CM/HP/SBE-β-CD-MNPs） nanocomposites were characterized by the TEM, FTIR, DLS, Zeta potential, XRD and VSM. In addition, the loading and release performance of the as-prepared nanocarriers for the hydrophobic anti-cancer drug curcumin was also investigated. The results revealed that the SBE-fl-CD-MNPs possessed the highest loading and release capacity in comparison with other two nanosystems. Cellular uptake and imaging suggested that the SBE-β-CD-MNPs entered into the cell, and curcumin could be suc-cessfully delivered into the cell by SBE-β-CD-MNPs nanocarrier. Moreover, cell toxicity experiments demonstrated the SBE-β-CD-MNPs were non-toxic, while curcumin loaded SBE-β-CD-MNPs showed high potential to kill the HepG2 cells. The as-prepared magnetic composites were expected to expand their potential applications in bio- medical field.展开更多
Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed pep...Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed peptide microarrays to map linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and vaccination.LPEs detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned with reported neutralizing antibody binding sies.Similar LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stimulus,where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function.Vaccine-specifc LPEs tended to map to sites known or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein.Mapping LPEs to regions of known functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.展开更多
The surface organs mainly comprise the superficial layers of various parts of the mammalian body,including the skin,eyes,and ears,which provide solid protection against various threats to the entire body.Damage to sur...The surface organs mainly comprise the superficial layers of various parts of the mammalian body,including the skin,eyes,and ears,which provide solid protection against various threats to the entire body.Damage to surface organs could lead to many serious diseases or even death.Currently,despite significant advancements in this field,there remain numerous enigmas that necessitate expeditious resolution,particularly pertaining to diagnostic and therapeutic objectives.The advancements in nanomedicine have provided a significant impetus for the development of novel approaches in the diagnosis,bioimaging,and therapy of superficial organs.The aggregation-induced emission(AIE)phenomenon,initially observed by Prof.Ben Zhong Tang,stands out due to its contrasting behavior to the aggregationcaused quenching effect.This discovery has significantly revolutionized the field of nanomedicine for surface organs owing to its remarkable advantages.In this review of literature,we aim to provide a comprehensive summary of recent advances of AIE lumenogen(AIEgen)-based nanoplatforms in the fields of detection,diagnosis,imaging,and therapeutics of surface organ-related diseases and discuss their prospects in the domain.It is hoped that this review will help attract researchers’attention toward the utilization of this field for the exploration of a wider range of biomedical and clinical applications.展开更多
文摘β-Cyclodextrin （β-CD） and its derivatives functionalized magnetic nanoparticles （MNPs） with high saturated magnetism were fabricated successfully by an effective grafting method. The resultant carboxymethyl/hydroxy- propyl/sulfobutyl ether-β-CD-MNPs （CM/HP/SBE-β-CD-MNPs） nanocomposites were characterized by the TEM, FTIR, DLS, Zeta potential, XRD and VSM. In addition, the loading and release performance of the as-prepared nanocarriers for the hydrophobic anti-cancer drug curcumin was also investigated. The results revealed that the SBE-fl-CD-MNPs possessed the highest loading and release capacity in comparison with other two nanosystems. Cellular uptake and imaging suggested that the SBE-β-CD-MNPs entered into the cell, and curcumin could be suc-cessfully delivered into the cell by SBE-β-CD-MNPs nanocarrier. Moreover, cell toxicity experiments demonstrated the SBE-β-CD-MNPs were non-toxic, while curcumin loaded SBE-β-CD-MNPs showed high potential to kill the HepG2 cells. The as-prepared magnetic composites were expected to expand their potential applications in bio- medical field.
基金supported by the Department of Defense(grant number W8IxwH19i0926)National Institute of Allergy and Infectious Diseases contract(grant number_HHSN2722017000331)+1 种基金National Institute of Child Health and Human Development grant(grant numbers R01HD090927 and R01HDi03511)National Center for Research Resources and the Ofice of Research Infrastructure Programs(grant numbers OD011104).
文摘Identification of epitopes targeted following virus infection or vaccination can guide vaccine design and development of therapeutic interventions targeting functional sites,but can be laborious.Herein,we employed peptide microarrays to map linear peptide epitopes(LPEs)recognized following SARS-CoV-2 infetion and vaccination.LPEs detected by nonhuman primate(NHP)and patient IgMs after SARS-CoV-2 infection extensively overlapped,localized to functionally important virus regions,and aligned with reported neutralizing antibody binding sies.Similar LPE overlap occurred atfter infection and vaccination,with LPE clusters specifc to each stimulus,where strong and conserved LPEs mapping to sites known or likely to inhibit spike protein function.Vaccine-specifc LPEs tended to map to sites known or likely to be afected by structural changes induced by the proline substitutions in the mRNA vaccine's S protein.Mapping LPEs to regions of known functional importance in this manner may acelerate vaccine evaluation and discovery of targets for sile secific therapeutic interventions.
基金National Natural Science Foundation of China,Grant/Award Number:82102904the Science,Technology&Innovation Commission of Shenzhen Municipality,Grant/Award Number:JCYJ20210324113405014+1 种基金Hunan Provincial Science and Technology Plan Project,Grant/Award Number:2021SK5001Natural Science and Health Bureau of Hunan Province,Grant/Award Number:2021JJ70136。
文摘The surface organs mainly comprise the superficial layers of various parts of the mammalian body,including the skin,eyes,and ears,which provide solid protection against various threats to the entire body.Damage to surface organs could lead to many serious diseases or even death.Currently,despite significant advancements in this field,there remain numerous enigmas that necessitate expeditious resolution,particularly pertaining to diagnostic and therapeutic objectives.The advancements in nanomedicine have provided a significant impetus for the development of novel approaches in the diagnosis,bioimaging,and therapy of superficial organs.The aggregation-induced emission(AIE)phenomenon,initially observed by Prof.Ben Zhong Tang,stands out due to its contrasting behavior to the aggregationcaused quenching effect.This discovery has significantly revolutionized the field of nanomedicine for surface organs owing to its remarkable advantages.In this review of literature,we aim to provide a comprehensive summary of recent advances of AIE lumenogen(AIEgen)-based nanoplatforms in the fields of detection,diagnosis,imaging,and therapeutics of surface organ-related diseases and discuss their prospects in the domain.It is hoped that this review will help attract researchers’attention toward the utilization of this field for the exploration of a wider range of biomedical and clinical applications.
