The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported.Gold nanodots,15 nm in diameter,arranged in large(cm2)square arrays with a pitch of 200 nm,are used as carr...The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported.Gold nanodots,15 nm in diameter,arranged in large(cm2)square arrays with a pitch of 200 nm,are used as carriers for primary antibodies(immunoglobulin G(IgG)),further recognized by secondary redox-labeled detection antibodies.Ensemble scale interrogation of the antibody array by cyclic voltammetry,and nanoscale interrogation of individual nanodots by mediator tethered atomic forcescanning electrochemical microscopy(Mt/AFM-SECM),enable the occupancy of nanodots by single antibody molecules to be demonstrated.Experiments involving the competitive adsorption of antibodies of different species onto the nanodots evidence the possibility of using single-antibody nanoarrays for digital electrochemical immunoassays.展开更多
文摘The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported.Gold nanodots,15 nm in diameter,arranged in large(cm2)square arrays with a pitch of 200 nm,are used as carriers for primary antibodies(immunoglobulin G(IgG)),further recognized by secondary redox-labeled detection antibodies.Ensemble scale interrogation of the antibody array by cyclic voltammetry,and nanoscale interrogation of individual nanodots by mediator tethered atomic forcescanning electrochemical microscopy(Mt/AFM-SECM),enable the occupancy of nanodots by single antibody molecules to be demonstrated.Experiments involving the competitive adsorption of antibodies of different species onto the nanodots evidence the possibility of using single-antibody nanoarrays for digital electrochemical immunoassays.
