Background:This study aimed to investigate the potential of intratumoral microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers as predictive biomarkers for antitumor efficacy in patients w...Background:This study aimed to investigate the potential of intratumoral microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers as predictive biomarkers for antitumor efficacy in patients with non-small cell lung cancer.Methods:This study observed patients with metastatic non-driver mutation non-small cell lung cancer who were initially diagnosed at the First Affiliated Hospital of Dalian Medical University’s Department of Oncology from August 2021 to July 2022 and completed at least four cycles of chemotherapy combined with immune checkpoint inhibitor treatment.Lung biopsy tissues,fecal,and peripheral blood samples were collected from these patients.Based on the efficacy of the combined chemotherapy and immunotherapy,patients were divided into an effective group and an ineffective group.The tumor microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers were compared between the two groups.The lung and fecal microbiota were analyzed using 16S rRNA high-throughput sequencing.Flow cytometry was used to detect T-cell subsets,and enzyme-linked immunosorbent assay was employed to measure inflammatory factors.Results:A total of 21 patients were observed.There were significant differences in the tumor microbiota between the responsive and non-responsive groups,particularly the proportion of the genera Sphingomonas and Pseudomonas.The gut microbiome composition changed after treatment,but there were no differences between the two groups before treatment.There were no significant differences in T-cell subsets and inflammatory markers between the responsive and non-responsive groups.Conclusion:The composition of intratumoral microbiota in non-small cell lung cancer patients may serve as an indicator of response to chemotherapy combined with immunotherapy.The predictive value of gut microbiota,T-cell subsets,and inflammatory markers appears limited.Future research should further validate the predictive role of changes in gut microbiota on treatment outcomes.展开更多
Chinese steel industry has made significant progress on reducing fresh water consumption and CO_2/SO_2/COD（ chemical oxygen demand） /dust emissions,and improving comprehensive utilization of solid waste. Some steel ...Chinese steel industry has made significant progress on reducing fresh water consumption and CO_2/SO_2/COD（ chemical oxygen demand） /dust emissions,and improving comprehensive utilization of solid waste. Some steel companies have become topped worldwide. However,due to the large output quantity of crude steel,the whole steel industry is still a huge source of pollutants. At present,environmental protection standards are encountering challenges of lack of technical support,taxation policy and other issues. Steel industry is currently facing enormous environmental pressure. The development trends of environmental protection technologies were studied by summarizing different development stages. To realize the development targets,the industry needs to carry out its research with independent intellectual property rights,develop comprehensive management systems and establish ecological chain with other industries. This can not only raise the level of environmental protection in a broader range,but also improve the energy efficiency of iron and steel plants and increase added value of waste utilization. Finally,23 environmental protection technologies were proposed and 4 new environmental protection practices were studied.展开更多
Air quenched basic oxygen furnace steel slag （BOF-SS） is processed at very high cooling rate, which is expected to have different cementitious properties from conventional slowly cooled BOF-SS. For this purpose, the...Air quenched basic oxygen furnace steel slag （BOF-SS） is processed at very high cooling rate, which is expected to have different cementitious properties from conventional slowly cooled BOF-SS. For this purpose, the strength activity indexes of air quenched and slowly cooled BOF-SS are investigated. The results reveal that, under the specific surface area （S） of 490 m^2/kg, the compressive strength activity index reaches 1.24 after 28 days with replacement of 15% air quenched BOF-SS and reaches 1.05 after 28 days with replacement of 20% air quenched BOF-SS and 30%granulated blast furnace slag （GBFS）. The cementitious activity of air quenched BOF-SS is obviously higher than that of slowly cooled BOF-SS, mainly because it contains more C3 S and glassy phases.展开更多
In the present study,we have prepared glutathione capped gold and platinum alloy nanoclusters(AuPtNCs)in a controlled way by employing the hydrothermal method and optimized through adjusting the ratio of raw materials...In the present study,we have prepared glutathione capped gold and platinum alloy nanoclusters(AuPtNCs)in a controlled way by employing the hydrothermal method and optimized through adjusting the ratio of raw materials,reaction temperature,and time.Compared with the corresponding monometallic gold and platinum nanoclusters,the alloy nanoclusters’catalytic activity is improved dramatically in the oxidation of 3,3,5,5-tetramethylbenzidine(TMB)in the presence of H_(2)O_(2).And the maximum velocity is calculated to be 106×10^(-8)M·s^(-1) for TMB as substrate,being much better than that of other reported metallic nanoclusters and nanoparticles.Further study shows that the high catalytic activity mainly attributes to the synergistic effect of gold and platinum.Besides,they have been applied to determine H_(2)O_(2)in the presence of TMB,which shows high sensitivity with a limit of detection(LOD)at 100 n M.The proposed method has been used to determine H_(2)O_(2)in milk and contact lens solutions,which shows very good recovery and exhibits high practical application potential.Therefore,the present study provides a new type of alloy nanoclusters with high peroxidase-like activity,which will inspire more research interests on doping and alloying with Pt to improve the catalytic activity of metal nanoclusters.展开更多
文摘Background:This study aimed to investigate the potential of intratumoral microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers as predictive biomarkers for antitumor efficacy in patients with non-small cell lung cancer.Methods:This study observed patients with metastatic non-driver mutation non-small cell lung cancer who were initially diagnosed at the First Affiliated Hospital of Dalian Medical University’s Department of Oncology from August 2021 to July 2022 and completed at least four cycles of chemotherapy combined with immune checkpoint inhibitor treatment.Lung biopsy tissues,fecal,and peripheral blood samples were collected from these patients.Based on the efficacy of the combined chemotherapy and immunotherapy,patients were divided into an effective group and an ineffective group.The tumor microbiota,gut microbiome,peripheral blood T-cell subsets,and inflammatory markers were compared between the two groups.The lung and fecal microbiota were analyzed using 16S rRNA high-throughput sequencing.Flow cytometry was used to detect T-cell subsets,and enzyme-linked immunosorbent assay was employed to measure inflammatory factors.Results:A total of 21 patients were observed.There were significant differences in the tumor microbiota between the responsive and non-responsive groups,particularly the proportion of the genera Sphingomonas and Pseudomonas.The gut microbiome composition changed after treatment,but there were no differences between the two groups before treatment.There were no significant differences in T-cell subsets and inflammatory markers between the responsive and non-responsive groups.Conclusion:The composition of intratumoral microbiota in non-small cell lung cancer patients may serve as an indicator of response to chemotherapy combined with immunotherapy.The predictive value of gut microbiota,T-cell subsets,and inflammatory markers appears limited.Future research should further validate the predictive role of changes in gut microbiota on treatment outcomes.
基金supported by National Natural Science Foundation of China(Grants No.51234003)
文摘Chinese steel industry has made significant progress on reducing fresh water consumption and CO_2/SO_2/COD（ chemical oxygen demand） /dust emissions,and improving comprehensive utilization of solid waste. Some steel companies have become topped worldwide. However,due to the large output quantity of crude steel,the whole steel industry is still a huge source of pollutants. At present,environmental protection standards are encountering challenges of lack of technical support,taxation policy and other issues. Steel industry is currently facing enormous environmental pressure. The development trends of environmental protection technologies were studied by summarizing different development stages. To realize the development targets,the industry needs to carry out its research with independent intellectual property rights,develop comprehensive management systems and establish ecological chain with other industries. This can not only raise the level of environmental protection in a broader range,but also improve the energy efficiency of iron and steel plants and increase added value of waste utilization. Finally,23 environmental protection technologies were proposed and 4 new environmental protection practices were studied.
基金Item Sponsored by National Natural Science Foundation of China(51234003)
文摘Air quenched basic oxygen furnace steel slag （BOF-SS） is processed at very high cooling rate, which is expected to have different cementitious properties from conventional slowly cooled BOF-SS. For this purpose, the strength activity indexes of air quenched and slowly cooled BOF-SS are investigated. The results reveal that, under the specific surface area （S） of 490 m^2/kg, the compressive strength activity index reaches 1.24 after 28 days with replacement of 15% air quenched BOF-SS and reaches 1.05 after 28 days with replacement of 20% air quenched BOF-SS and 30%granulated blast furnace slag （GBFS）. The cementitious activity of air quenched BOF-SS is obviously higher than that of slowly cooled BOF-SS, mainly because it contains more C3 S and glassy phases.
基金financially supported by financial support from the National Natural Science Foundation of China(Nos.21875085,21373101,and 91027027)the Opening Program of the State Key Laboratory of Supramolecular Structure and Materials,Jilin University。
文摘In the present study,we have prepared glutathione capped gold and platinum alloy nanoclusters(AuPtNCs)in a controlled way by employing the hydrothermal method and optimized through adjusting the ratio of raw materials,reaction temperature,and time.Compared with the corresponding monometallic gold and platinum nanoclusters,the alloy nanoclusters’catalytic activity is improved dramatically in the oxidation of 3,3,5,5-tetramethylbenzidine(TMB)in the presence of H_(2)O_(2).And the maximum velocity is calculated to be 106×10^(-8)M·s^(-1) for TMB as substrate,being much better than that of other reported metallic nanoclusters and nanoparticles.Further study shows that the high catalytic activity mainly attributes to the synergistic effect of gold and platinum.Besides,they have been applied to determine H_(2)O_(2)in the presence of TMB,which shows high sensitivity with a limit of detection(LOD)at 100 n M.The proposed method has been used to determine H_(2)O_(2)in milk and contact lens solutions,which shows very good recovery and exhibits high practical application potential.Therefore,the present study provides a new type of alloy nanoclusters with high peroxidase-like activity,which will inspire more research interests on doping and alloying with Pt to improve the catalytic activity of metal nanoclusters.
