The Qaidam Basin(QB)is a concentrated distribution area and chemical industrial bases of salt lakes in China.Lakes in the QB have been expanding during the past 20 years.Rapid lake expansion resulted in some considera...The Qaidam Basin(QB)is a concentrated distribution area and chemical industrial bases of salt lakes in China.Lakes in the QB have been expanding during the past 20 years.Rapid lake expansion resulted in some considerable scientific issues on the protection of salt lake resources and infrastructure,and monitoring of hydrological processes at the lake-basin scale.Although the spatial-temporal trends of lake changes on the Qinghai-Tibet Plateau(QTP)have been well documented,the underlying influencing mechanism and hydrogeological implications of rapid lake changes in the QB are not well understood.Three lakes in the northern QB were selected to investigate lake water level fluctuations on different time scales based on extensive in-situ monitoring and satellite observations.The influencing mechanism and hydrogeological implications of rapid changes of terminal lakes were discussed in combination with the reported increasing precipitation rate and mass balance of glaciers in the northern QTP.Results reveal the following:(1)the fluctuation pattern of Sugan Lake was asynchronous and out of phase with that of Xiao Qaidam and Toson lakes during the monitoring period;(2)Sugan Lake water rose gradually,and the rise interval was from late April to early July.In contrast,Xiao Qaidam and Toson lakes took on a rapid and steep rise,and the rise intervalwas from late July to September;(3)the influencing mechanisms for rapid lake fluctuations are controlled by different factors:glacier and snow melting with increasing temperature for Sugan Lake and increasing precipitation for Xiao Qaidam and Toson lakes;(4)in accordance with different intervals and influencing mechanisms of rapid lake expansions in the QB,hydrological risk precaution of lakes and corresponding river catchments was conducted in different parts of the basin.This study provided an important scientific basis for assessing the hydrological process and hydrological risk precaution,and protection of salt lake resources along with rapid lake expansions in the arid area.展开更多
The Qarhan Salt Lake(QSL)in western China is K-Sr-Li-B-Br-Rb multi-resource coexisting Quaternary brine deposits.Significant research efforts have been directed to the origin of K-Li resources and evolutionary history...The Qarhan Salt Lake(QSL)in western China is K-Sr-Li-B-Br-Rb multi-resource coexisting Quaternary brine deposits.Significant research efforts have been directed to the origin of K-Li resources and evolutionary history of the QSL.However,the study on the different sources,recharge processes,and differential distribution patterns for these resource elements in brine deposits is still inadequate.Therefore,we measured Li-B concentrations and H-O-Sr-B isotopic compositions of different waters(river,spring,and brine)from the QSL,combined with the reported K-Sr contents and multiple isotopes of waters,to discuss the recharge,source of K-Sr-Li-B and their spatial distributions,by analogy with other evaporite basins in the world.The results show that:(1)the K-Li-B-Sr elemental concentrations of brines and their spatial distribution in the QSL are diverse;(2)high K and Sr values are distributed in Dabuxun and Qarhan sections,respectively,which are controlled by Ca-Cl springs in the northern QSL;on the contrary,Li and B values are enriched in the Bieletan section and are charged by thermal springs in the Kunlun Mountains;(3)the formation and evolution of Ca-Cl and thermal springs constrain fundamentally on the recharge processes of K-Sr and Li-B elements in the terminal salt lakes of the Qaidam Basin(QB);(4)some analogues of recharge processes limit the resource elements of the QSL and other salt lakes(Da Qaidam,Lop Nur,Zhabuye,Atacama,and Guayatayoc)in the world provides a reference for the resource exploration in deep formation waters in the evaporite basins.展开更多
Accurate determination of biological activity is essential in quality control of recombinant human brain natriuretic peptide(rhBNP). In previous study, we successfully developed a genetically modified cell line 293GCA...Accurate determination of biological activity is essential in quality control of recombinant human brain natriuretic peptide(rhBNP). In previous study, we successfully developed a genetically modified cell line 293GCAC3-based ELISA assay for rhBNP. But ELISA procedure is still tedious, so this study was aimed to develop a rapid and simple bioassay for rhBNP using GloSensor technology, which provides a platform of flexible luciferase-based biosensors for real-time detection of signaling events in live cells, including cGMP production. A reporter cell line 293GCAGlo-G1 was constructed by transfecting pGloSensor^(TM) 40 F plasmid into 293GCAC3. The reporter assay based on 293GCAGlo-G1 showed high precision with intraassay CV being 8.3% and inter-assay CV being 14.1%; high accuracy with 80%, 100% and 120% recovery rate being 99.2%, 102.4% and 99.0% respectively; and great linearity with R^2 of linear fitting equation being 0.99. Besides, no significant difference was found in test results of reporter assay and 293GCAC3-based ELISA assay(paired t test, p=0.630). All these results suggested that the reporter assay was a viable assay for biological determination of rhBNP.展开更多
基金Supported by the Second Tibetan Plateau Scientific Expedition and Research Program(No.2019 QZKK 0805)the National Natural Science Foundation of China(No.U 21 A 2018)the Foundation of Department of Qinghai Science&Technology(No.2020-ZJ-T 06)。
文摘The Qaidam Basin(QB)is a concentrated distribution area and chemical industrial bases of salt lakes in China.Lakes in the QB have been expanding during the past 20 years.Rapid lake expansion resulted in some considerable scientific issues on the protection of salt lake resources and infrastructure,and monitoring of hydrological processes at the lake-basin scale.Although the spatial-temporal trends of lake changes on the Qinghai-Tibet Plateau(QTP)have been well documented,the underlying influencing mechanism and hydrogeological implications of rapid lake changes in the QB are not well understood.Three lakes in the northern QB were selected to investigate lake water level fluctuations on different time scales based on extensive in-situ monitoring and satellite observations.The influencing mechanism and hydrogeological implications of rapid changes of terminal lakes were discussed in combination with the reported increasing precipitation rate and mass balance of glaciers in the northern QTP.Results reveal the following:(1)the fluctuation pattern of Sugan Lake was asynchronous and out of phase with that of Xiao Qaidam and Toson lakes during the monitoring period;(2)Sugan Lake water rose gradually,and the rise interval was from late April to early July.In contrast,Xiao Qaidam and Toson lakes took on a rapid and steep rise,and the rise intervalwas from late July to September;(3)the influencing mechanisms for rapid lake fluctuations are controlled by different factors:glacier and snow melting with increasing temperature for Sugan Lake and increasing precipitation for Xiao Qaidam and Toson lakes;(4)in accordance with different intervals and influencing mechanisms of rapid lake expansions in the QB,hydrological risk precaution of lakes and corresponding river catchments was conducted in different parts of the basin.This study provided an important scientific basis for assessing the hydrological process and hydrological risk precaution,and protection of salt lake resources along with rapid lake expansions in the arid area.
基金Supported by the Second Tibetan Plateau Scientific Expedition and Research Program(No.2019 QZKK 0805)the National Natural Science Foundation of China(No.U 21 A 2018)the Foundation of Department of Qinghai Science&Technology(No.2020-ZJ-T 06)。
文摘The Qarhan Salt Lake(QSL)in western China is K-Sr-Li-B-Br-Rb multi-resource coexisting Quaternary brine deposits.Significant research efforts have been directed to the origin of K-Li resources and evolutionary history of the QSL.However,the study on the different sources,recharge processes,and differential distribution patterns for these resource elements in brine deposits is still inadequate.Therefore,we measured Li-B concentrations and H-O-Sr-B isotopic compositions of different waters(river,spring,and brine)from the QSL,combined with the reported K-Sr contents and multiple isotopes of waters,to discuss the recharge,source of K-Sr-Li-B and their spatial distributions,by analogy with other evaporite basins in the world.The results show that:(1)the K-Li-B-Sr elemental concentrations of brines and their spatial distribution in the QSL are diverse;(2)high K and Sr values are distributed in Dabuxun and Qarhan sections,respectively,which are controlled by Ca-Cl springs in the northern QSL;on the contrary,Li and B values are enriched in the Bieletan section and are charged by thermal springs in the Kunlun Mountains;(3)the formation and evolution of Ca-Cl and thermal springs constrain fundamentally on the recharge processes of K-Sr and Li-B elements in the terminal salt lakes of the Qaidam Basin(QB);(4)some analogues of recharge processes limit the resource elements of the QSL and other salt lakes(Da Qaidam,Lop Nur,Zhabuye,Atacama,and Guayatayoc)in the world provides a reference for the resource exploration in deep formation waters in the evaporite basins.
基金supported by grants from the National Science and Technology Major Project(No.2015ZX09501008-001)the Middle-aged and Young Development Research Foundation of NIFDC(No.2017B3)
文摘Accurate determination of biological activity is essential in quality control of recombinant human brain natriuretic peptide(rhBNP). In previous study, we successfully developed a genetically modified cell line 293GCAC3-based ELISA assay for rhBNP. But ELISA procedure is still tedious, so this study was aimed to develop a rapid and simple bioassay for rhBNP using GloSensor technology, which provides a platform of flexible luciferase-based biosensors for real-time detection of signaling events in live cells, including cGMP production. A reporter cell line 293GCAGlo-G1 was constructed by transfecting pGloSensor^(TM) 40 F plasmid into 293GCAC3. The reporter assay based on 293GCAGlo-G1 showed high precision with intraassay CV being 8.3% and inter-assay CV being 14.1%; high accuracy with 80%, 100% and 120% recovery rate being 99.2%, 102.4% and 99.0% respectively; and great linearity with R^2 of linear fitting equation being 0.99. Besides, no significant difference was found in test results of reporter assay and 293GCAC3-based ELISA assay(paired t test, p=0.630). All these results suggested that the reporter assay was a viable assay for biological determination of rhBNP.
