Dissolved oxygen(DO)is one of most important factors which affect wide range physiologic features of including immune responses and intestinal bacterial community.However,the underlying mechanisms remain enigmatic.To ...Dissolved oxygen(DO)is one of most important factors which affect wide range physiologic features of including immune responses and intestinal bacterial community.However,the underlying mechanisms remain enigmatic.To address this question,the intestinal bacterial community compositions and the immune features of Atlantic salmon(Salmo salar)grown in recirculating aquaculture systems(RAS)were characterized.Fish were reared under different DO saturation levels,e.g.,200% saturation named high group(H),100%saturation named control group(CK),and 60%saturation named lower group(L).Large variations in the operational taxonomic units(OTUs)frequency distribution for the intestinal bacterial community of Atlantic salmon were observed.The intestinal bacterial community of all groups was dominated mainly by three phyla,e.g.,Proteobacteria.Firmicutes,and Bacteroidetes.Interestingly,Acinetobacter baumannii,an opportunistic pathogen of salmon was increased significantly in L group.We further monitored the immunity features of fish under different DO levels.The results show that leucocyte number,cortisol level,the expressions of interleukin-1β(IL-1β),Toll-like receptor 4(TLR4),and nucleotide-binding oligomerization domain like protein 2(NOD2)were higher at significant levels in the L group than those in the other two groups.TLR4 and NOD2 are usually related with the bacterial infections;therefore,it is reasonable to believe that the stronger immune responses observed in the L group might be related with the higher abundance of A.baumannii in the inte stine of Atlantic salmon.Overall,these findings demonstrated that low DO level may induce stronger immunity responses in Atlantic salmon.展开更多
Objective:To explore the effect of continuous non-invasive blood pressure monitoring on intraoperative hemodynamics and postoperative myocardial injury in craniotomy.Methods:120 cases of elective craniotomy were divid...Objective:To explore the effect of continuous non-invasive blood pressure monitoring on intraoperative hemodynamics and postoperative myocardial injury in craniotomy.Methods:120 cases of elective craniotomy were divided into the self-control group(continuous non-invasive blood pressure monitoring and intermittent cuff non-invasive blood pressure monitoring,CNAP group)and propensity score matching group(only intermittent cuff non-invasive blood pressure measurement in previous craniotomy,PSM group);Goal-directed hemodynamic management in CNAP group included heart rate(HR),blood pressure(BP),stroke volume(SV),stroke variability(SVV),and systemic vascular resistance index(SVRI).The main index is to compare the troponin level within 72 hours after operation between the CNAP group and the PSM group;The secondary indicators are the comparison of the hemodynamic conditions between the CNAP group and the PSM at 10 specific time points.Results:The incidence of postoperative myocardial injury in the CNAP group was significantly lower than that in the PSM group(12%vs.30%,P=0.01);in the CNAP group hypotensive episodes(6 vs.3,P=0.01),positive balance of fluid therapy(700 vs.500 mL,P<0.001),more use of vasoactive drugs(29 vs.18,P=0.04),more stable hemodynamics medical status(P=0.03)were recorded.Conclusion:The hemodynamic management strategy based on continuous non-invasive blood pressure monitoring can reduce the incidence of myocardial injury after elective craniotomy and maintain a more stable hemodynamic state.展开更多
Objective:To explore the half-effective dose(ED_(50))of remazolam toluenesulfonate combined with subthreshold amounts of esketamine for inhibiting cardiovascular response to tracheal intubation in elderly patients.Met...Objective:To explore the half-effective dose(ED_(50))of remazolam toluenesulfonate combined with subthreshold amounts of esketamine for inhibiting cardiovascular response to tracheal intubation in elderly patients.Method:We included 42 patients,aged 65-75,who required general anesthesia and single-lumen endotracheal intubation for elective surgery.The first patient was administered remazolam toluenesulfonate at a dose of 0.20 mg/kg.Once the patient lost consciousness,their alertness/sedation score(OAA/S score)was≤1,and their BIS score was≤60,and a subthreshold dose(0.3 mg/kg)of esketamine was given.The subsequent doses were adjusted using a sequential approach based on the cardiovascular response to tracheal intubation observed in the previous patient.The dose was modified in increments or decrements of 0.01 mg/kg.The ED_(50)and 95%CI of remazolam toluenesulfonate were calculated using the Dixon and Massey sequential distribution test method.Result:The inhibition of endotracheal intubation response was positively correlated with the dose of remazolam toluenesulfonate,and the depth of sedation could not be achieved when the amount was≤0.22 mg/kg.The ED_(50)of remazolam toluenesulfonate combined with a subthreshold dose of esketamine in inhibiting cardiovascular response to tracheal intubation in elderly patients was 0.30(0.28,0.33)mg/kg.There was no statistically significant difference in blood pressure between the induction of anesthesia and before the operation.Conclusion:When compounded with 0.3 mg/kg esketamine,the ED_(50)of Remazolam toluenesulfonate in inhibiting cardiovascular response to endotracheal intubation in elderly patients was 0.30 mg/kg(95%CI0.28-0.33 mg/kg).展开更多
Evapotranspiration(ET) within an ecosystem is crucial for the water-limited environment that currently lacks adequate quantification in the arid region of Northwest China, mainly covered by phreatophytes, such as the ...Evapotranspiration(ET) within an ecosystem is crucial for the water-limited environment that currently lacks adequate quantification in the arid region of Northwest China, mainly covered by phreatophytes, such as the Populus euphratica Oliv. tree and the Tamarix ramosissima Ledeb. shrub species. Accordingly, ET was measured for an entire year using eddy covariance(EC) in P. euphratica stands in the lower Heihe River Basin, Northwest China. During the growing season,the total ET was 850 mm, with a mean of 4.0 mm/d, which is obviously more than that observed at tree-level and standlevel scales, which was likely due to the different level of soil evaporation induced by irrigation via water conveyance.Factors associated with ET fall into either environmental or plant eco-physiological categories. Environmental factors account for at least 79% variation of ET, and the linear relationship between ET and the groundwater table(GWT) revealed the potential water use of P. euphratica forests under the non-water stress condition with the GWT less than 3 m deep.Plant eco-physiological parameters, specifically the leaf area index(LAI), have direct impact on the seasonal pattern of ET, which provides a valuable reference to the wide-area estimates of ET for riparian forests by using LAI. In conclusion,P. euphratica forests have high water use after water conveyance, which may be the result of long-term adapting to local climates and limited water availability.展开更多
Evidence exists of nighttime transpiration and its potential impact on plant/water relations for species in a diversity of ecosystems. However, relevant data related to typical desert riparian forest species remains l...Evidence exists of nighttime transpiration and its potential impact on plant/water relations for species in a diversity of ecosystems. However, relevant data related to typical desert riparian forest species remains limited Accordingly, we measured sap flow velocity of Populus euphratica using the heat ratio method between 2012 and2014. Nocturnal stem sap flow was separated into nighttime and stem refilling using the ‘‘forecasted refilling''method. Nighttime transpiration was observed for each phenophase. The highest value was during the full foliation period but lowest during leaf expansion and defoliation periods. The contribution of nighttime transpiration to daytime transpiration was an average of 15% but this was comparatively higher during the defoliation period. Relationships between nighttime transpiration, vapor pressure deficits, and air temperatures were more closely associated than with wind speed in all phenophases. Moreover, we found that nighttime transpiration linearly correlated to vapour pressure deficit during the first and the full foliation periods, but nighttime transpiration showed exponential correlations to air temperatures during the same phenophases. Additionally, environmental drivers of transpiration were significantly different between nighttime and daytime(P \ 0.05). Driving forces behind nighttime transpiration were characterized by many factors, and integrated impacts between these multiple environmental factors were complex. Future studies should focus on these integrated impacts on nighttime transpiration, and the physiological mechanisms of nighttime transpiration should be investigated, given that this could also influence its occurrence and magnitude during different phenophases.展开更多
Understanding forest ecosystem evapotranspiration(ET) is crucial for water-limited environments,particularly those that lack adequate quantified data such as the lower Heihe River basin of northwest China which is pri...Understanding forest ecosystem evapotranspiration(ET) is crucial for water-limited environments,particularly those that lack adequate quantified data such as the lower Heihe River basin of northwest China which is primarily dominated by Tamarix ramosissima Ledeb.and Populus euphratica Oliv.forests.Accordingly,we selected the growing season for 2 years (2012 and 2014) of two such forests under similar meteorological conditions to compare ET using the eddy covariance(EC) technique.During the growing seasons,daily ET of T.ramosissima ranged from 0.3 to 8.0 mm day^(-1) with a mean of 3.6 mm day^(-1),and daily ET of P.euphratica ranged from 0.9 to 7.9 mm day^(-1) with a mean of 4.6 mm day^(-1) for a total of 548 and 707 mm,respectively.The significantly higher ET of the P.euphratica stand was directly linked to high soil evaporation rates under sufficient water availability from irrigation.When the soil evaporation was disregarded,water use was comparable to two contrasting riparian forests,a P.euphratica forest with a total transpiration of 465 mm and a T.ramosissima forest with 473 mm.Regression analysis demonstrated that climate factors accounted for at least 80% of ET variation in both forest types.In conclusion,water use of the riparian forests was low and comparable in this arid region,that suggest the long-term plant adaptation to the local climate and conditions of water availability.展开更多
The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and...The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.展开更多
In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause chan...In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning time.In this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 c.Distinct expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and eye.The most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal development.In addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and maturation.The results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal reproduction.Moreover,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation.展开更多
Ecophysiological responses to drought stress of Populus euphratica in Alashan Desert Eco-hydrology Experimental Research Station were investigated.Results show that under mild and moderate drought stress,stomatal leng...Ecophysiological responses to drought stress of Populus euphratica in Alashan Desert Eco-hydrology Experimental Research Station were investigated.Results show that under mild and moderate drought stress,stomatal length,aperture,area and density is likely to decrease in the early days,but afterwards this is likely to recovery with treatment over the pas‐sage of treatment time.Under severe drought stress,these properties appear to decline continuously.However,after 45 days of drought-stress treatment,the decline is not as noticeable as before,indicating that Populus euphratica could possibly reduce water evaporation by shutting down the stoma,leading to an improvement in its water use efficiency with better survival under drought stress conditions.The leaf area first decreases,and then increases under mild and moderate drought stress conditions,with the average values under different degree of stress found to be approximately 129.52,120.08,116.63 and 107.28 cm2,respectively.Under moderate stress conditions,the leaf water potential appears to show a continuous decline where the average values under different degree of stress are found to be-1.27,-1.85,-4.29 and-4.80 MPa,respectively.In terms of proline content,the results demonstrate that this factor appears to increase significant‐ly under moderate and severe drought stress conditions.Especially under severe drought stress condition,the content is found to be more than 700μg/g.Ranging over average values of 14.64 and 15.90 nmol/g under moderate and severe drought stress,respectively,Malondialdehyde content is found to increase quite rapidly under moderate and severe drought stress conditions at first,which then appears to decrease gradually with the treatment over time.展开更多
With the greenhouse soil that had been planted with cucumber as the control,the greenhouse soil that had been planted with pepper for 3,7,10 and 15 consecutive years was collected,and the effects of continuous croppin...With the greenhouse soil that had been planted with cucumber as the control,the greenhouse soil that had been planted with pepper for 3,7,10 and 15 consecutive years was collected,and the effects of continuous cropping on plant height,stem diameter,leaf area,yield,photosynthetic pigment content and fruit quality of pepper were investigated by pot experiment. The results showed that with the extension of continuous cropping duration,the plant height,stem diameter and yield of pepper decreased significantly compared with the control; and the mass fraction of photosynthetic pigments declined,and the mass fractions of chlorophyll a and total chlorophyll were significantly lower than those of the control. Under the condition of continuous cropping,the fruit quality of pepper decreased significantly,and the mass fractions of soluble solids and soluble protein were significantly lower than those of the control.展开更多
Objective:To explore the effects of different infusion schemes on colloid osmotic pressure during major abdominal surgery and perioperative albumin in elderly patients.Methods:140 elderly patients of 65-80 years old u...Objective:To explore the effects of different infusion schemes on colloid osmotic pressure during major abdominal surgery and perioperative albumin in elderly patients.Methods:140 elderly patients of 65-80 years old undergoing major abdominal surgery were divided into 4 groups according to the method of random number table,and different proportions of crystals and colloids were given to different groups(group A:full crystal,group B:crystal-colloid ratio 1:1,group C:crystal-colloid ratio 2:1,Group D:crystalcolloid ratio 1:2).The plasma colloid osmotic pressure and level of albumin during perioperative period were monitored.Simultaneously observe the arterial blood pH,blood glucose,extubating time of endotracheal tube,postoperative feeding time,et al.Results:The total amount of liquid infusion in the group of whole crystal was 3056ml(3056±253),which was significantly more than other groups(P<0.01).At the same time,the colloid osmotic pressure decreased by 11.9 mmHg(11.9±2.8),which was more obviously decreased than that of the other groups(B group3.9±1.3,C group 1.5±0.3,D group 4.7±2.1).The difference was a statistically significant(P<0.01).On the next day after surgery,the level of albumin decreased by an average of 4.3 g/L(4.5±1.9)compared with that in group B before surgery,and group C decreased by 2.9 g/L(2.9±1.2)in average,which was significantly different(P<0.05)from group A 10.2 g/L(10.2±1.8).There was no statistically significant difference between group A and group D(P>0.05).And the other indexes were not significantly different between the two groups.Conclusion:This study found that different infusion solutions with different crystal-colloid ratios had an effect on perioperative colloid osmotic pressure and level of albumin.展开更多
Objective:To explore the effects of different positions on supine hypotensive syndrome in cesarean section after lumbar anesthesia.Methods:600 fullterm parturient were randomly divided into 4 groups.The patented posit...Objective:To explore the effects of different positions on supine hypotensive syndrome in cesarean section after lumbar anesthesia.Methods:600 fullterm parturient were randomly divided into 4 groups.The patented positioning pads(patent number:ZL 201720618886.5)in our department was used when the left-leaning position was placed.The parturient lied in a supine position for anesthesia,and then the group of positioning pads was placed after turning into the lateral position.The parturient were divided into group A(supine position),group B(left-leaning to 10°),group C(left-leaning to 20°),and group D(30°).Observation index:Main index:Comparison of maternal blood pressure changes and neonatal blood gas analysis in the supine position without using position pad and with the use of patent positioning pads in different tilt angles(10°,20°,30°).Minor index:1)the use and frequency of vasoactive drugs,whether a left-leaning operating bed or uterine displacement is required;2)the selffeeling of the parturient;3)newborn’s Apgar scores of 1 minute,5 minutes,and 10 minutes after birth;4)whether the obstetrician can perform the operation smoothly in the corresponding left leaning position.Results:The blood pressure at the supine position after anesthesia,the beginning of surgery and the time when the newborn was delivered in group A and group B were significantly different from those in group C(P<0.05).There were significant differences at different timings in group A and group B,and the decline was more significant at the supine position after anesthesia,the beginning of surgery and the time when the newborn was delivered(P<0.05).The pH of blood gas of newborns in group A,group B and group C was between 7.25 and 7.37,and there was no significant difference among the three groups(P>0.05).Conclusion:The use of positioning pads can prevent the adverse effects of supine hypotensive syndrome on parturient and newborns to a certain extent.展开更多
World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmenta...World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmental factors[1].展开更多
Purpose:To analyze the application path of the integration of clinical medicine integrated courses with the elements of ideology and politics under the theory of“Sanquan Education,”and to provide references for the ...Purpose:To analyze the application path of the integration of clinical medicine integrated courses with the elements of ideology and politics under the theory of“Sanquan Education,”and to provide references for the promotion of educational reform in medical schools.Methods:60 clinical medicine undergraduates at Jiamusi University in 2018 and 2019 were randomly selected as research subjects.On the basis of the analysis of students’characteristics and pre-study content,the students were divided into the integration group and the traditional group,each with 30 students.Students in the integration group carried out clinical medicine integrated class with the integration of ideological and political elements.Students in the traditional group carried out the traditional clinical medicine integrated class.Classroom evaluation scales,anonymous questionnaires,and interviews were used to conduct research,collect data,and compare and observe the application effects.Results:After carrying out different modes of clinical medicine integrated courses,the undergraduates of the Excellent Physician Class in the integration group were higher than those in the traditional group in terms of teaching satisfaction,comprehensive quality,basic knowledge test,and case analysis scores through questionnaire analysis(P<0.05).In the evaluation of the effect of ideological education,the undergraduates of the Excellent Physician Class of the integration group were higher than the traditional group in terms of the acceptance of doctor-patient communication,the correct rate of mastering the history of the discipline,and the correct rate of mastering the frontiers and policies(P<0.05).Conclusion:Through the integration of clinical medicine integrated course and ideological and political elements,we can help students establish correct values while teaching them professional knowledge,improve the implementation of moral education in medical schools,cultivate excellent medical workers with high morality and firm beliefs for the society,and realize the fundamental educational task of establishing morality and educating people in medical schools in the context of“Sanquan Education.”展开更多
The printable electrode interlayer with excellent thickness tolerance is crucial for mass production of organic solar cells(OSCs)by solution-based print techniques. Herein, high-quality printable SnO2 films are simply...The printable electrode interlayer with excellent thickness tolerance is crucial for mass production of organic solar cells(OSCs)by solution-based print techniques. Herein, high-quality printable SnO2 films are simply fabricated by spin-coating or bladecoating the chemical precipitated SnO2 colloid precursor with post thermal annealing treatment. The SnO2 films possess outstanding optical and electrical properties, especially extreme thickness-insensitivity. The interfacial electron trap density of SnO2 cathode interlayers(CILs) are very low and show negligible increase as the thicknesses increase from 10 to 160 nm,resulting in slight change of the power conversion efficiencies(PCEs) of the PM6:Y6 based OSCs from 16.10% to 13.07%. For blade-coated SnO2 CIL, the PCE remains high up to 12.08% even the thickness of SnO2 CIL is high up to 530 nm. More strikingly, the large-area OSCs of 100 mm2 with printed SnO2 CILs obtain a high efficiency of 12.74%. To the best of our knowledge, this work presents the first example for the high-performance and large-area OSCs with the thickness-insensitive SnO2 CIL.展开更多
The increasing production and usage of chlorinated paraffins(CPs) correspondently increase the amount of CPs that experience thermal processes. Our previous study revealed that a significant amount of medium-chain chl...The increasing production and usage of chlorinated paraffins(CPs) correspondently increase the amount of CPs that experience thermal processes. Our previous study revealed that a significant amount of medium-chain chlorinated paraffins(MCCPs), short-chain chlorinated paraffins(SCCPs) as well as aromatic and chlorinated polycyclic aromatic hydrocarbons(Cl-PAHs) were formed synergistically during the thermal decomposition of CP-52(a class of CP products).However, the transformation mechanisms of CP-52 to these compounds are still not very clear.This article presents a mechanistic analysis on the decomposition of CP-52 experimentally and theoretically. It was found that CP-52 initially undergoes dehydrochlorination and carbon chain cleavage and it transformed into chlorinated and unsaturated hydrocarbons. Cyclization and aromatization were the most accessible pathways at low temperatures(200–400°C), both of which produce mostly aromatic hydrocarbons. As the temperature exceeds 400°C, the hydrocarbons could decompose into small molecules, and the subsequent radical-induced reactions become the predominant pathways, leading to the formation of Cl-PAHs. The decomposition of CP-52 was investigated by using density functional theory and calculations demonstrating the feasibility and rationality of PCB and PCN formation from chlorobenzene. The results improve the understanding of the transformation processes from CP-52 to SCCPs and Cl-PAHs as well as provide data for reducing their emissions during thermal-related processes.展开更多
Coffee is one of the most important agricultural commodities and has the unique organoleptic characteristics such as strong but not bitter taste,fragrant,oily,and fruity.In this study,an untargeted metabolomics approa...Coffee is one of the most important agricultural commodities and has the unique organoleptic characteristics such as strong but not bitter taste,fragrant,oily,and fruity.In this study,an untargeted metabolomics approach based on UHPLC-QE-MS was used to investigate the differences in terms of components of precursor metabolites in coffee beans from 18 producing regions worldwide.Fingerprint analysis,principal component analysis and hierarchical clustering analysis revealed a neat separation among coffee beans.Compounds with high relevance to variance in the projection values in supervised multivariate analysis were selected as important metabolites for the discrimination of coffee samples.In total,10 different families of compounds were considered as potential markers of the coffee beans:3-hydroxycoumarin,4,5-di-O-caffeoylquinic,cryptochlorogenic acid,palmitic amide,linoleamide,arachidic acid,petroselinic acid,trehalose,L-glutamic acid,L-malic acid.The findings presented herein serve as a suitable framework for the design of novel discrimination strategies in food origin tracing.展开更多
What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is ...What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems.展开更多
基金Supported by the Shandong Province Key Research and Invention Program(No.2017CXGC010K)the China Agriculture Research System(No.CARS-47)+7 种基金the National Key Research and Development Program(No.2018YFD0901204)the National Infrastructure of Fishery Germplasm Resource(No.2019DKA30470)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0502-2)the National Natural Science Foundation of China(Nos.31872606,31572657,U1701233,31402283,31802319)the Department of Agriculture and Rural Areas of Guangdong Province(No.KA1911101)the Natural Science Foundation of Shandong Province(No.ZR2018BC053)the Agricultural Application Technology Innovation Project of Shandong Province in 2018/2019(No.SD2019YY011)the Shandong Province Major Scientific and Technological Innovation Projects(No.2019JZZY020710)。
文摘Dissolved oxygen(DO)is one of most important factors which affect wide range physiologic features of including immune responses and intestinal bacterial community.However,the underlying mechanisms remain enigmatic.To address this question,the intestinal bacterial community compositions and the immune features of Atlantic salmon(Salmo salar)grown in recirculating aquaculture systems(RAS)were characterized.Fish were reared under different DO saturation levels,e.g.,200% saturation named high group(H),100%saturation named control group(CK),and 60%saturation named lower group(L).Large variations in the operational taxonomic units(OTUs)frequency distribution for the intestinal bacterial community of Atlantic salmon were observed.The intestinal bacterial community of all groups was dominated mainly by three phyla,e.g.,Proteobacteria.Firmicutes,and Bacteroidetes.Interestingly,Acinetobacter baumannii,an opportunistic pathogen of salmon was increased significantly in L group.We further monitored the immunity features of fish under different DO levels.The results show that leucocyte number,cortisol level,the expressions of interleukin-1β(IL-1β),Toll-like receptor 4(TLR4),and nucleotide-binding oligomerization domain like protein 2(NOD2)were higher at significant levels in the L group than those in the other two groups.TLR4 and NOD2 are usually related with the bacterial infections;therefore,it is reasonable to believe that the stronger immune responses observed in the L group might be related with the higher abundance of A.baumannii in the inte stine of Atlantic salmon.Overall,these findings demonstrated that low DO level may induce stronger immunity responses in Atlantic salmon.
文摘Objective:To explore the effect of continuous non-invasive blood pressure monitoring on intraoperative hemodynamics and postoperative myocardial injury in craniotomy.Methods:120 cases of elective craniotomy were divided into the self-control group(continuous non-invasive blood pressure monitoring and intermittent cuff non-invasive blood pressure monitoring,CNAP group)and propensity score matching group(only intermittent cuff non-invasive blood pressure measurement in previous craniotomy,PSM group);Goal-directed hemodynamic management in CNAP group included heart rate(HR),blood pressure(BP),stroke volume(SV),stroke variability(SVV),and systemic vascular resistance index(SVRI).The main index is to compare the troponin level within 72 hours after operation between the CNAP group and the PSM group;The secondary indicators are the comparison of the hemodynamic conditions between the CNAP group and the PSM at 10 specific time points.Results:The incidence of postoperative myocardial injury in the CNAP group was significantly lower than that in the PSM group(12%vs.30%,P=0.01);in the CNAP group hypotensive episodes(6 vs.3,P=0.01),positive balance of fluid therapy(700 vs.500 mL,P<0.001),more use of vasoactive drugs(29 vs.18,P=0.04),more stable hemodynamics medical status(P=0.03)were recorded.Conclusion:The hemodynamic management strategy based on continuous non-invasive blood pressure monitoring can reduce the incidence of myocardial injury after elective craniotomy and maintain a more stable hemodynamic state.
文摘Objective:To explore the half-effective dose(ED_(50))of remazolam toluenesulfonate combined with subthreshold amounts of esketamine for inhibiting cardiovascular response to tracheal intubation in elderly patients.Method:We included 42 patients,aged 65-75,who required general anesthesia and single-lumen endotracheal intubation for elective surgery.The first patient was administered remazolam toluenesulfonate at a dose of 0.20 mg/kg.Once the patient lost consciousness,their alertness/sedation score(OAA/S score)was≤1,and their BIS score was≤60,and a subthreshold dose(0.3 mg/kg)of esketamine was given.The subsequent doses were adjusted using a sequential approach based on the cardiovascular response to tracheal intubation observed in the previous patient.The dose was modified in increments or decrements of 0.01 mg/kg.The ED_(50)and 95%CI of remazolam toluenesulfonate were calculated using the Dixon and Massey sequential distribution test method.Result:The inhibition of endotracheal intubation response was positively correlated with the dose of remazolam toluenesulfonate,and the depth of sedation could not be achieved when the amount was≤0.22 mg/kg.The ED_(50)of remazolam toluenesulfonate combined with a subthreshold dose of esketamine in inhibiting cardiovascular response to tracheal intubation in elderly patients was 0.30(0.28,0.33)mg/kg.There was no statistically significant difference in blood pressure between the induction of anesthesia and before the operation.Conclusion:When compounded with 0.3 mg/kg esketamine,the ED_(50)of Remazolam toluenesulfonate in inhibiting cardiovascular response to endotracheal intubation in elderly patients was 0.30 mg/kg(95%CI0.28-0.33 mg/kg).
基金supported by the Youth Foundation of the National Natural Science Foundation of China (41401033)the Chinese Postdoctoral Science Foundation (2014M560819)+1 种基金the General Program of the National Natural Science Fund of China (Nos. 31370466, 41271037)the Natural Science Foundation of Gansu Province (No. 145RJZA141)
文摘Evapotranspiration(ET) within an ecosystem is crucial for the water-limited environment that currently lacks adequate quantification in the arid region of Northwest China, mainly covered by phreatophytes, such as the Populus euphratica Oliv. tree and the Tamarix ramosissima Ledeb. shrub species. Accordingly, ET was measured for an entire year using eddy covariance(EC) in P. euphratica stands in the lower Heihe River Basin, Northwest China. During the growing season,the total ET was 850 mm, with a mean of 4.0 mm/d, which is obviously more than that observed at tree-level and standlevel scales, which was likely due to the different level of soil evaporation induced by irrigation via water conveyance.Factors associated with ET fall into either environmental or plant eco-physiological categories. Environmental factors account for at least 79% variation of ET, and the linear relationship between ET and the groundwater table(GWT) revealed the potential water use of P. euphratica forests under the non-water stress condition with the GWT less than 3 m deep.Plant eco-physiological parameters, specifically the leaf area index(LAI), have direct impact on the seasonal pattern of ET, which provides a valuable reference to the wide-area estimates of ET for riparian forests by using LAI. In conclusion,P. euphratica forests have high water use after water conveyance, which may be the result of long-term adapting to local climates and limited water availability.
基金financially supported by the Key Research Program of Frontier Sciences CAS(QYZDJ-SSWDQC031)Key Project of the Chinese Academy of Sciences(KZZDEW-04-05)+1 种基金the National Natural Science Foundation of China(91025024)the ‘‘Western Light’’ project of the Chinese Academy of Science
文摘Evidence exists of nighttime transpiration and its potential impact on plant/water relations for species in a diversity of ecosystems. However, relevant data related to typical desert riparian forest species remains limited Accordingly, we measured sap flow velocity of Populus euphratica using the heat ratio method between 2012 and2014. Nocturnal stem sap flow was separated into nighttime and stem refilling using the ‘‘forecasted refilling''method. Nighttime transpiration was observed for each phenophase. The highest value was during the full foliation period but lowest during leaf expansion and defoliation periods. The contribution of nighttime transpiration to daytime transpiration was an average of 15% but this was comparatively higher during the defoliation period. Relationships between nighttime transpiration, vapor pressure deficits, and air temperatures were more closely associated than with wind speed in all phenophases. Moreover, we found that nighttime transpiration linearly correlated to vapour pressure deficit during the first and the full foliation periods, but nighttime transpiration showed exponential correlations to air temperatures during the same phenophases. Additionally, environmental drivers of transpiration were significantly different between nighttime and daytime(P \ 0.05). Driving forces behind nighttime transpiration were characterized by many factors, and integrated impacts between these multiple environmental factors were complex. Future studies should focus on these integrated impacts on nighttime transpiration, and the physiological mechanisms of nighttime transpiration should be investigated, given that this could also influence its occurrence and magnitude during different phenophases.
基金supported by the National Natural Science Foundation of China(41401033,31370466,and 41271037)the China Postdoctoral Science Foundation(2014M560819)the National Key Research and Development Program of China(2016YFC0501002)
文摘Understanding forest ecosystem evapotranspiration(ET) is crucial for water-limited environments,particularly those that lack adequate quantified data such as the lower Heihe River basin of northwest China which is primarily dominated by Tamarix ramosissima Ledeb.and Populus euphratica Oliv.forests.Accordingly,we selected the growing season for 2 years (2012 and 2014) of two such forests under similar meteorological conditions to compare ET using the eddy covariance(EC) technique.During the growing seasons,daily ET of T.ramosissima ranged from 0.3 to 8.0 mm day^(-1) with a mean of 3.6 mm day^(-1),and daily ET of P.euphratica ranged from 0.9 to 7.9 mm day^(-1) with a mean of 4.6 mm day^(-1) for a total of 548 and 707 mm,respectively.The significantly higher ET of the P.euphratica stand was directly linked to high soil evaporation rates under sufficient water availability from irrigation.When the soil evaporation was disregarded,water use was comparable to two contrasting riparian forests,a P.euphratica forest with a total transpiration of 465 mm and a T.ramosissima forest with 473 mm.Regression analysis demonstrated that climate factors accounted for at least 80% of ET variation in both forest types.In conclusion,water use of the riparian forests was low and comparable in this arid region,that suggest the long-term plant adaptation to the local climate and conditions of water availability.
基金The National Natural Science Foundation of China under contract Nos 31802319,31972784,31802269the Natural Science Foundation of Shandong Province under contract No.ZR2018BC053+1 种基金the Agricultural Application Technology Innovation Project of Shandong Province under contract No.SD2019YY006the Advanced Talents Foundation of Qingdao Agricultural University under contract Nos 6631119055,6631119032.
文摘The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.
基金The National Natural Science Foundation of China under contract No.31802319the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)under contract No.GML2019ZD0402+7 种基金the National Key Research and Development Program under contract No.2018YFD0901204the Major Agricultural Application Technology Innovation Project of Shandong Province under contract No.SD2019YY011the Natural Science Foundation of Shandong Province under contract No.ZR2018BC053the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2018SDKJ0502-2the Fund of China Agriculture Research System under contract No.CARS-47the Major Science and Technology for Scientific and Technological Innovation Projects(Shandong)under contract No.2019JZZY020710the Science and Technology Service Network Initiative Project under contract Nos KFZD-SW-106,ZSSD-019,2017T3017 and 2019T3022the Advanced Talents Foundation of Qingdao Agricultural University under contract No.6631119055。
文摘In most fish,reproduction is seasonal or periodic under the suitable conditions.In turbot(Scophthalmus maximus)farms,one of the most economically important marine flatfish species,changes in daylength could cause changes in the spawning time.In this study,to characterize the regulation of reproductive physiology following light signals,three melatonin receptors(Mtnr)investigated in turbot were named sm Mtnr1,sm Mtnr2,and sm Mtnr1 c.Distinct expression profiles demonstrated that Mtnr m RNAs were concentrated in the brain(as detected in the hypothalamus(Hy)and mesencephalon(Me)),gonad and eye.The most abundant Mtnr1 and Mtnr2 m RNA expression levels were detected in the central nervous system at the beginning of the breeding season,suggesting that Mtnr1 and Mtnr2 may play vital roles in the regulation of turbot gonadal development.In addition,the melatonin profiles gradually increased and reached to the highest level at the spawning stage,indicating that melatonin is a potent hormone in the regulation of fish oocyte growth and maturation.The results of this study suggested that melatonin is the primary factor that transduces the light signal and regulates the physiological functions of turbot seasonal reproduction.Moreover,the results of this study may establish a foundation for further research seeking to identify fish melatonin receptors involved in the gonadal development and gamete maturation.
基金the National Natural Science Foundation of China"Nighttime tran-spiration and its eco-hydrological effects in typical desert vegetation"(42001038)the Major special projects of science and technology in Inner Mongolia Autonomous Region(zdzx2018057)+1 种基金the project of CAS innovation cross team(JCTD-2019-19)The authors thank anonymous reviewers for their valuable review and constructive comments on this manuscript.
文摘Ecophysiological responses to drought stress of Populus euphratica in Alashan Desert Eco-hydrology Experimental Research Station were investigated.Results show that under mild and moderate drought stress,stomatal length,aperture,area and density is likely to decrease in the early days,but afterwards this is likely to recovery with treatment over the pas‐sage of treatment time.Under severe drought stress,these properties appear to decline continuously.However,after 45 days of drought-stress treatment,the decline is not as noticeable as before,indicating that Populus euphratica could possibly reduce water evaporation by shutting down the stoma,leading to an improvement in its water use efficiency with better survival under drought stress conditions.The leaf area first decreases,and then increases under mild and moderate drought stress conditions,with the average values under different degree of stress found to be approximately 129.52,120.08,116.63 and 107.28 cm2,respectively.Under moderate stress conditions,the leaf water potential appears to show a continuous decline where the average values under different degree of stress are found to be-1.27,-1.85,-4.29 and-4.80 MPa,respectively.In terms of proline content,the results demonstrate that this factor appears to increase significant‐ly under moderate and severe drought stress conditions.Especially under severe drought stress condition,the content is found to be more than 700μg/g.Ranging over average values of 14.64 and 15.90 nmol/g under moderate and severe drought stress,respectively,Malondialdehyde content is found to increase quite rapidly under moderate and severe drought stress conditions at first,which then appears to decrease gradually with the treatment over time.
基金Supported by Youth Science and Technology Foundation of Gansu Province(1610RJYE145)Agricultural Science and Technology Innovation Fund of Gansu Academy of Agricultural Sciences(2017GAAS51)Science and Technology Support Plan of Tianshui City(2018-NCK-6711)
文摘With the greenhouse soil that had been planted with cucumber as the control,the greenhouse soil that had been planted with pepper for 3,7,10 and 15 consecutive years was collected,and the effects of continuous cropping on plant height,stem diameter,leaf area,yield,photosynthetic pigment content and fruit quality of pepper were investigated by pot experiment. The results showed that with the extension of continuous cropping duration,the plant height,stem diameter and yield of pepper decreased significantly compared with the control; and the mass fraction of photosynthetic pigments declined,and the mass fractions of chlorophyll a and total chlorophyll were significantly lower than those of the control. Under the condition of continuous cropping,the fruit quality of pepper decreased significantly,and the mass fractions of soluble solids and soluble protein were significantly lower than those of the control.
文摘Objective:To explore the effects of different infusion schemes on colloid osmotic pressure during major abdominal surgery and perioperative albumin in elderly patients.Methods:140 elderly patients of 65-80 years old undergoing major abdominal surgery were divided into 4 groups according to the method of random number table,and different proportions of crystals and colloids were given to different groups(group A:full crystal,group B:crystal-colloid ratio 1:1,group C:crystal-colloid ratio 2:1,Group D:crystalcolloid ratio 1:2).The plasma colloid osmotic pressure and level of albumin during perioperative period were monitored.Simultaneously observe the arterial blood pH,blood glucose,extubating time of endotracheal tube,postoperative feeding time,et al.Results:The total amount of liquid infusion in the group of whole crystal was 3056ml(3056±253),which was significantly more than other groups(P<0.01).At the same time,the colloid osmotic pressure decreased by 11.9 mmHg(11.9±2.8),which was more obviously decreased than that of the other groups(B group3.9±1.3,C group 1.5±0.3,D group 4.7±2.1).The difference was a statistically significant(P<0.01).On the next day after surgery,the level of albumin decreased by an average of 4.3 g/L(4.5±1.9)compared with that in group B before surgery,and group C decreased by 2.9 g/L(2.9±1.2)in average,which was significantly different(P<0.05)from group A 10.2 g/L(10.2±1.8).There was no statistically significant difference between group A and group D(P>0.05).And the other indexes were not significantly different between the two groups.Conclusion:This study found that different infusion solutions with different crystal-colloid ratios had an effect on perioperative colloid osmotic pressure and level of albumin.
文摘Objective:To explore the effects of different positions on supine hypotensive syndrome in cesarean section after lumbar anesthesia.Methods:600 fullterm parturient were randomly divided into 4 groups.The patented positioning pads(patent number:ZL 201720618886.5)in our department was used when the left-leaning position was placed.The parturient lied in a supine position for anesthesia,and then the group of positioning pads was placed after turning into the lateral position.The parturient were divided into group A(supine position),group B(left-leaning to 10°),group C(left-leaning to 20°),and group D(30°).Observation index:Main index:Comparison of maternal blood pressure changes and neonatal blood gas analysis in the supine position without using position pad and with the use of patent positioning pads in different tilt angles(10°,20°,30°).Minor index:1)the use and frequency of vasoactive drugs,whether a left-leaning operating bed or uterine displacement is required;2)the selffeeling of the parturient;3)newborn’s Apgar scores of 1 minute,5 minutes,and 10 minutes after birth;4)whether the obstetrician can perform the operation smoothly in the corresponding left leaning position.Results:The blood pressure at the supine position after anesthesia,the beginning of surgery and the time when the newborn was delivered in group A and group B were significantly different from those in group C(P<0.05).There were significant differences at different timings in group A and group B,and the decline was more significant at the supine position after anesthesia,the beginning of surgery and the time when the newborn was delivered(P<0.05).The pH of blood gas of newborns in group A,group B and group C was between 7.25 and 7.37,and there was no significant difference among the three groups(P>0.05).Conclusion:The use of positioning pads can prevent the adverse effects of supine hypotensive syndrome on parturient and newborns to a certain extent.
基金supported by the National Natural Science Foundation of China(22136006,22021003)the K.C.Wong Education Foundation of China(GJTD-2020-03)。
文摘World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmental factors[1].
基金Heilongjiang Province Education Science Planning Key Project(Project number:GJB1423157)。
文摘Purpose:To analyze the application path of the integration of clinical medicine integrated courses with the elements of ideology and politics under the theory of“Sanquan Education,”and to provide references for the promotion of educational reform in medical schools.Methods:60 clinical medicine undergraduates at Jiamusi University in 2018 and 2019 were randomly selected as research subjects.On the basis of the analysis of students’characteristics and pre-study content,the students were divided into the integration group and the traditional group,each with 30 students.Students in the integration group carried out clinical medicine integrated class with the integration of ideological and political elements.Students in the traditional group carried out the traditional clinical medicine integrated class.Classroom evaluation scales,anonymous questionnaires,and interviews were used to conduct research,collect data,and compare and observe the application effects.Results:After carrying out different modes of clinical medicine integrated courses,the undergraduates of the Excellent Physician Class in the integration group were higher than those in the traditional group in terms of teaching satisfaction,comprehensive quality,basic knowledge test,and case analysis scores through questionnaire analysis(P<0.05).In the evaluation of the effect of ideological education,the undergraduates of the Excellent Physician Class of the integration group were higher than the traditional group in terms of the acceptance of doctor-patient communication,the correct rate of mastering the history of the discipline,and the correct rate of mastering the frontiers and policies(P<0.05).Conclusion:Through the integration of clinical medicine integrated course and ideological and political elements,we can help students establish correct values while teaching them professional knowledge,improve the implementation of moral education in medical schools,cultivate excellent medical workers with high morality and firm beliefs for the society,and realize the fundamental educational task of establishing morality and educating people in medical schools in the context of“Sanquan Education.”
基金supported by the National Natural Science Foundation of China (51873007, 51961165102, 21835006)the Fundamental Research Funds for the Central Universities in China (2019MS025, 2018MS032, 2017MS027, 2017XS084)。
文摘The printable electrode interlayer with excellent thickness tolerance is crucial for mass production of organic solar cells(OSCs)by solution-based print techniques. Herein, high-quality printable SnO2 films are simply fabricated by spin-coating or bladecoating the chemical precipitated SnO2 colloid precursor with post thermal annealing treatment. The SnO2 films possess outstanding optical and electrical properties, especially extreme thickness-insensitivity. The interfacial electron trap density of SnO2 cathode interlayers(CILs) are very low and show negligible increase as the thicknesses increase from 10 to 160 nm,resulting in slight change of the power conversion efficiencies(PCEs) of the PM6:Y6 based OSCs from 16.10% to 13.07%. For blade-coated SnO2 CIL, the PCE remains high up to 12.08% even the thickness of SnO2 CIL is high up to 530 nm. More strikingly, the large-area OSCs of 100 mm2 with printed SnO2 CILs obtain a high efficiency of 12.74%. To the best of our knowledge, this work presents the first example for the high-performance and large-area OSCs with the thickness-insensitive SnO2 CIL.
基金supported by the National Natural Science Foundation of China (Nos.21625702,21337002,21621064)the National Basic Research Program of China (No.2015CB453102)the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDB14010400)
文摘The increasing production and usage of chlorinated paraffins(CPs) correspondently increase the amount of CPs that experience thermal processes. Our previous study revealed that a significant amount of medium-chain chlorinated paraffins(MCCPs), short-chain chlorinated paraffins(SCCPs) as well as aromatic and chlorinated polycyclic aromatic hydrocarbons(Cl-PAHs) were formed synergistically during the thermal decomposition of CP-52(a class of CP products).However, the transformation mechanisms of CP-52 to these compounds are still not very clear.This article presents a mechanistic analysis on the decomposition of CP-52 experimentally and theoretically. It was found that CP-52 initially undergoes dehydrochlorination and carbon chain cleavage and it transformed into chlorinated and unsaturated hydrocarbons. Cyclization and aromatization were the most accessible pathways at low temperatures(200–400°C), both of which produce mostly aromatic hydrocarbons. As the temperature exceeds 400°C, the hydrocarbons could decompose into small molecules, and the subsequent radical-induced reactions become the predominant pathways, leading to the formation of Cl-PAHs. The decomposition of CP-52 was investigated by using density functional theory and calculations demonstrating the feasibility and rationality of PCB and PCN formation from chlorobenzene. The results improve the understanding of the transformation processes from CP-52 to SCCPs and Cl-PAHs as well as provide data for reducing their emissions during thermal-related processes.
基金This work was supported by Risk assessment of agricultural product quality and safety-Building a coffee quality index system[grant number 125D0202].
文摘Coffee is one of the most important agricultural commodities and has the unique organoleptic characteristics such as strong but not bitter taste,fragrant,oily,and fruity.In this study,an untargeted metabolomics approach based on UHPLC-QE-MS was used to investigate the differences in terms of components of precursor metabolites in coffee beans from 18 producing regions worldwide.Fingerprint analysis,principal component analysis and hierarchical clustering analysis revealed a neat separation among coffee beans.Compounds with high relevance to variance in the projection values in supervised multivariate analysis were selected as important metabolites for the discrimination of coffee samples.In total,10 different families of compounds were considered as potential markers of the coffee beans:3-hydroxycoumarin,4,5-di-O-caffeoylquinic,cryptochlorogenic acid,palmitic amide,linoleamide,arachidic acid,petroselinic acid,trehalose,L-glutamic acid,L-malic acid.The findings presented herein serve as a suitable framework for the design of novel discrimination strategies in food origin tracing.
基金Supported by the National Key Research and Development Program of China(2021YFC2301000).
文摘What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems.
