Heat shock protein 90(HSP90)represents a suite of highly conserved and multi-functional molecular chaperone proteins that play an important role in cellular stress responses.In order to better understand the expressio...Heat shock protein 90(HSP90)represents a suite of highly conserved and multi-functional molecular chaperone proteins that play an important role in cellular stress responses.In order to better understand the expression of HSP90 in mollusks,a full-length complementary DNA(cDNA)of HSP90(HcHSP90)was identified in Hyriopsis cumingii.HcHSP90 cDNA was 2659 bp in length,consisting of 30 and 50-untranslated regions and an open reading frame of 2187 bp,that encoded a 728 amino acid protein.Homology analyses showed that the HcHSP90 protein was highly conserved and had 5 well-conserved family signatures of HSP90 proteins.HcHSP90 mRNA expressed in various tissues of H.cumingii.The expression level of HcHSP90 was the highest in the digestive gland.In all tissues,with the exception of the digestive gland where it was down-regulated,HcHSP90 mRNA expression was significantly induced by temperature treatments(0,5,25,and 35℃)relative to the control(15℃).Exposure of H.cumingii to different concentrations of cadmium(50,100,and 200 mg/L),up-regulated HcHSP90 mRNA in the haemolymph and gill but without an obvious dose-dependent response.When H.cumingii were infected with Aeromonas hydrophila,HcHSP90 mRNA expression in the haemolymph was up-regulated and peaked 36 h post-infection,while in the gills it was significantly up-regulated 3 h post-infection in the gills,then remained constant until returning to pre-challenge expression levels at 36 h post-infection.The results show that HcHSP90 expression can be significantly regulated by changes in temperature,cadmium exposure and bacterial infection.We deduced that HSP90 may play an important role in helping H.cumingii to cope with environmental stress.展开更多
The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study...The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study,the full-length cDNA of the fem-1c(Hcfem-1c)gene was isolated from the freshwater mussel(Hypriopsis cumingii).The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains.Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues,indicating that the sequence of HcFEM-1C was conserved during evolution.Quantitative real-time PCR(qPCR)expression revealed that the Hcfem-1c gene was expressed in the adductor muscle,foot,liver,gill,kidney,mantle,and gonads of male and female adult mussels(two years old).In the gonads Hcfem-1c was much less abundant in males than that in females.During early development of the gonads,Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage(5 months old).We hypothesized that Hcfem-1c probably regulates female gonad differentiation.In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall,indicating that Hcfem-1c gene may not only be involved in female gonad differentiation,but also participates in egg development.This study laid the foundations for a better understanding of gender differentiation mechanism in H.cumingii.展开更多
基金This study was supported by the National Natural Science Foundation of China(31101939)the Key Fundamental Research Project of Shanghai Municipal Science and Technology Committee(10JC1406300)the Innovation Program of Shanghai Municipal Education Commission(13ZZ128).
文摘Heat shock protein 90(HSP90)represents a suite of highly conserved and multi-functional molecular chaperone proteins that play an important role in cellular stress responses.In order to better understand the expression of HSP90 in mollusks,a full-length complementary DNA(cDNA)of HSP90(HcHSP90)was identified in Hyriopsis cumingii.HcHSP90 cDNA was 2659 bp in length,consisting of 30 and 50-untranslated regions and an open reading frame of 2187 bp,that encoded a 728 amino acid protein.Homology analyses showed that the HcHSP90 protein was highly conserved and had 5 well-conserved family signatures of HSP90 proteins.HcHSP90 mRNA expressed in various tissues of H.cumingii.The expression level of HcHSP90 was the highest in the digestive gland.In all tissues,with the exception of the digestive gland where it was down-regulated,HcHSP90 mRNA expression was significantly induced by temperature treatments(0,5,25,and 35℃)relative to the control(15℃).Exposure of H.cumingii to different concentrations of cadmium(50,100,and 200 mg/L),up-regulated HcHSP90 mRNA in the haemolymph and gill but without an obvious dose-dependent response.When H.cumingii were infected with Aeromonas hydrophila,HcHSP90 mRNA expression in the haemolymph was up-regulated and peaked 36 h post-infection,while in the gills it was significantly up-regulated 3 h post-infection in the gills,then remained constant until returning to pre-challenge expression levels at 36 h post-infection.The results show that HcHSP90 expression can be significantly regulated by changes in temperature,cadmium exposure and bacterial infection.We deduced that HSP90 may play an important role in helping H.cumingii to cope with environmental stress.
基金This work was supported by the National Natural Science Foundation of China[grant number 31772835]the Innovation Program of Shanghai Municipal Education Commission[grant number 13ZZ128]the Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding[grant number ZF1206].
文摘The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study,the full-length cDNA of the fem-1c(Hcfem-1c)gene was isolated from the freshwater mussel(Hypriopsis cumingii).The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains.Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues,indicating that the sequence of HcFEM-1C was conserved during evolution.Quantitative real-time PCR(qPCR)expression revealed that the Hcfem-1c gene was expressed in the adductor muscle,foot,liver,gill,kidney,mantle,and gonads of male and female adult mussels(two years old).In the gonads Hcfem-1c was much less abundant in males than that in females.During early development of the gonads,Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage(5 months old).We hypothesized that Hcfem-1c probably regulates female gonad differentiation.In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall,indicating that Hcfem-1c gene may not only be involved in female gonad differentiation,but also participates in egg development.This study laid the foundations for a better understanding of gender differentiation mechanism in H.cumingii.
