BACKGROUND Severe craniocerebral injury(STBI)is a critical physical trauma caused by a sudden external force acting on the head.The condition is complex and changeable,and disability and mortality rates are high.Altho...BACKGROUND Severe craniocerebral injury(STBI)is a critical physical trauma caused by a sudden external force acting on the head.The condition is complex and changeable,and disability and mortality rates are high.Although the life of STBI patients can be saved through treatment,the sequelae of consciousness,speech,cognitive impairment,stiffness,spasm,pain and abnormal behavior in the early rehabilitation stage can be a heavy burden to a family.In the past,routine nursing was often used to treat/manage STBI;however,problems,such as improper cooperation and untimely communication,reduced therapeutic effectiveness.AIM To investigate the effect of a proposed care bundle to optimize the first aid process and assess its effectiveness on the early rehabilitation nursing of patients with STBI.METHODS From January 2019 to December 2020,126 STBI patients were admitted to the emergency department of Chongqing Emergency Medical Center.These patients were retrospectively selected as the research participants in the current study.The study participants were then divided into a control group(61 cases)and a study group(65 cases).The control group was treated with routine nursing.The study group adopted the proposed care bundle.The National Institutes of Health Stroke Scale/Score and Glasgow Coma Scale(GCS)were used to evaluate neurological function before and after emergency treatment.After 3 mo of rehabilitation,experimental outcomes were assessed.These included the GCS,Barthel Index,complication rate,muscle strength grade and satisfaction.RESULTS There was no significant difference in gender,age,cause of injury and GCS between the two groups.After emergency,the National Institutes of Health Stroke Scale/Score of the study group(10.23±3.26)was lower than that of the control group(14.79±3.14).The GCS score of the study group(12.48±2.38)was higher than that of the control group(9.32±2.01).The arrival time of consultation in the study group was 20.56±19.12,and the retention time in the emergency room was 45.12±10.21,which were significantly shorter than those in the control group.After 3 mo of rehabilitation management,the GCS and Barthel Index of the study group were 14.56±3.75 and 58.14±12.14,respectively,which were significantly higher than those of the control group.The incidence of complications in the study group(15.38%)was significantly lower than that in the control group(32.79%).The proportion of muscle strength≥grade III in the study group(89.23%)was significantly higher than that in the control group(50.82%).The satisfaction of patients in the study group was significantly higher than that in the control group.CONCLUSION Care bundles are used to optimize the nursing process.During first-aid,care bundles can effectively improve the rescue effect and improve neurological function of STBI patients as well as shorten the treatment time.In early rehabilitation,they can effectively improve the consciousness of STBI patients,improve the activities of daily living,reduce the risk of complications,accelerate the recovery of muscle strength and improve their satisfaction.展开更多
Drought at the grain filling stage of wheat will cause premature leaf senescence, thus leading to considerable loss of wheat yield. Therefore, this paper aims to establish a cultivation technology for strong drought r...Drought at the grain filling stage of wheat will cause premature leaf senescence, thus leading to considerable loss of wheat yield. Therefore, this paper aims to establish a cultivation technology for strong drought resistance, delayed senescence, and yield improvement based on the analysis of hormones homeostasis obtained by applying chemical control substances. Experiments were conducted with two genotypes of wheat. Four water irrigation treatments were applied to impose the water deficit, including well-watered control treatment(WW), mild water deficit(MiWD), moderate water deficit(MoWD), and severe water deficit(SWD). Exogenous abscisic acid(ABA) was sprayed on the plants at the anthesis stage of the wheat. As a result, exogenous ABA reduced initial senescence rate(r0), total duration of chlorophyll(Chltotal), rapid senescence phase(Chlloss), and the accumulated temperature at an inflection point(M) but improved the persistence phase(Chlper) of flag leaves under all of the four treatments. However, exogenous ABA produced inconsistent effects on photoassimilate relocation and grain weight under different treatments. It produced positive regulatory effects on grain weight under WW, MiWD, and MoWD treatments. On the one hand, spraying ABA during the persistence phase of flag leaves reduced the ratios of zeatin to gibberellin(Z/GA3), spermine to spermidine(Spm/Spd), and salicylic acid to ABA(SA/ABA),which prolonged active photosynthesis by stimulating high level of proline(Pro) and increased the activities of antioxidant enzymes, such as superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX). Therefore, drought tolerance was enhanced, and more photosynthetic assimilates were accumulated. On the other hand, the rapid senescence phase and the transport rate of assimilates into grains were accelerated, resulting in higher grain weight, yield, and water use efficiency(WUE). However, under SWD treatment, exogenous ABA improved the ratio of SA/ABA, leading to low Pro content and low antioxidant enzyme activity of flag leaves in the rapid loss phase. Meanwhile,drought resistance declined and the transport duration of assimilates into grains was shortened, thus making photosynthetic assimilates redundant. Therefore, exogenous ABA can lead to the reduction in grain weight, yield, and WUE of wheat under SWD treatment.展开更多
To optimize milling force and machining accuracy quality in corner milling process, the changing law of milling force is revealed by Finite Element Method（FEM）. Based on DEFORM software a serial of 3D FEM models for...To optimize milling force and machining accuracy quality in corner milling process, the changing law of milling force is revealed by Finite Element Method（FEM）. Based on DEFORM software a serial of 3D FEM models for corner milling process are devloped. Tool curved trajectory is achieved by establishing accurate relationship of tool location with milling time. Adaptive remeshing technique and iterative algorithm are adopted to ensure convergence of FEM model. Component force characteristics are revealed by analyzing FEM simulation results. It indicates that the milling force in Y direction becomes negative comparing with forces in X and Z direction. Magnitude of forces in three directions decreases with increase of spindle speed, while it increases with increase of milling feedrate. The simulation results for cutting force are in good agreement with those obtained from experiment. The FEM simulation model is first successfully established for corner milling process in this study, and the results provide a guide for optimizing cutting parameters in cutting process.展开更多
Objective:To investigate the clinical study of Qiming granule combined with compound anisodine injection in the treatment of diabetic retinopathy.Methods:104 patients with nonproliferative diabetic retinopathy admitte...Objective:To investigate the clinical study of Qiming granule combined with compound anisodine injection in the treatment of diabetic retinopathy.Methods:104 patients with nonproliferative diabetic retinopathy admitted to department of ophthalmology in Xishui Hospital Affiliated to Hubei University of science and technology from September 2016 to May 2018 were selected as the study subjects.According to the principle of treatment,they were divided into 52 cases in the control group and 52 cases in the treatment group,the clinical efficacy,blood sugar and lipid levels,hemorheological changes and adverse reactions were compared between the two groups.Results:The total effective rate difference between the treatment group(92.31%)and the control group(71.15%)was statistically significant(χ^(2)=7.792,P=0.005).After treatment,the two groups of fasting plasma glucose(FPG),hemoglobin A1c(HbA1c),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C),whole blood high-viscosity,whole blood cut viscosity,whole blood low-cut viscosity,plasma viscosity,number of microangioma and area of the hemorrhage were significantly lower than before treatment(P<0.05),and the levels of FPG,HbA1c,TC,LDL-C,whole blood highviscosity,whole blood cut viscosity,whole blood low-cut viscosity,plasma viscosity,number of microangioma and area of the hemorrhage in the treatment group were significantly lower than those in the control group(χ^(2)=0.122,P=0.727).Conclusions:Qiming granule combined with compound anisodine injection can effectively improve the clinical efficacy of patients with non-proliferative DR,and its mechanism may be related to improve blood sugar and blood lipid levels and correct blood rheology disorder,reduce microangioma and hemorrhage.It has clinical promotion significance.展开更多
Periodontitis is an inflammatory disease caused by bacterial biofilms,which leads to the destruction of periodontal tissue.Current treatments,such as mechanical cleaning and antibiotics,struggle to effectively address...Periodontitis is an inflammatory disease caused by bacterial biofilms,which leads to the destruction of periodontal tissue.Current treatments,such as mechanical cleaning and antibiotics,struggle to effectively address the persistent biofilms,inflammation,and tissue damage.A new approach involves developing a Janus nanomotor(J-CeM@Au)by coating cerium dioxide-doped mesoporous silica(CeM)with gold nanoparticles(AuNPs).This nanomotor exhibits thermophoretic motion when exposed to near-infrared(NIR)laser light due to the temperature gradient produced by the photothermal effects of asymmetrically distributed AuNPs.The NIR laser provides the energy for propulsion and activates the nanomotor's antibacterial properties,allowing it to penetrate biofilms and kill bacteria.Additionally,the nanomotor's ability to scavenge reactive oxygen species(ROS)can modulate the immune response and create a regenerative environment,promoting the healing of periodontal tissue.Overall,this multifunctional nanomotor offers a promising new approach for treating periodontitis by simultaneously addressing biofilm management and immune modulation with autonomous movement.展开更多
The sharing of pathogens is of great significance for the continuous and comprehensive testing and monitoring of viral samples,vaccine and drug development,and the study of drug resistance and mutability of viral samp...The sharing of pathogens is of great significance for the continuous and comprehensive testing and monitoring of viral samples,vaccine and drug development,and the study of drug resistance and mutability of viral samples.This paper reviews the current legal framework in the field of pathogen sharing,analyzes existing issues,and proposes recommendations to improve the legal framework of pathogen sharing.展开更多
Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,interven...Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.展开更多
As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed den...As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed dentinal tubules provide channels for irritants and bacterial invasion,leading to dentin hypersensitivity and even pulp inflammation.Cariogenic bacterial adhesion and biofilm formation on dentin are responsible for tooth demineralization and caries.It remains a clinical challenge to achieve the integration of tubule occlusion,collagen mineralization,and antibiofilm functions for managing exposed dentin.To address this issue,an epigallocatechin-3-gallate(EGCG)and poly(allylamine)-stabilized amorphous calcium phosphate(PAH-ACP)co-delivery hollow mesoporous silica(HMS)nanosystem(E/PA@HMS)was herein developed.The application of E/PA@HMS effectively occluded the dentinal tubules with acid-and abrasion-resistant stability and inhibited the biofilm formation of Streptococcus mutans.Intrafibrillar mineralization of collagen fibrils and remineralization of demineralized dentin were induced by E/PA@HMS.The odontogenic differentiation and mineralization of dental pulp cells with high biocompatibility were also promoted.Animal experiments showed that E/PA@HMS durably sealed the tubules and inhibited biofilm growth up to 14 days.Thus,the development of the E/PA@HMS nanosystem provides promising benefits for protecting exposed dentin through the coordinated manipulation of dentin caries and hypersensitivity.展开更多
The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,...The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,3 days)in Fig.5c were mistakenly used during figure assembly.We tracked down the original data obtained in June 2022,and have replaced Fig.5 with the correct image as follows.The authors would like to apologise for any inconvenience caused and state that the correction does not change the scientific conclusions of the article in any way.展开更多
Mapping of three-dimensional network on chip is a key problem in the research of three-dimensional network on chip. The quality of the mapping algorithm used di- rectly affects the communication efficiency between IP ...Mapping of three-dimensional network on chip is a key problem in the research of three-dimensional network on chip. The quality of the mapping algorithm used di- rectly affects the communication efficiency between IP cores and plays an important role in the optimization of power consumption and throughput of the whole chip. In this paper, ba- sic concepts and related work of three-dimensional network on chip are introduced. Quantum-behaved particle swarm op- timization algorithm is applied to the mapping problem of three-dimensional network on chip for the first time. Sim- ulation results show that the mapping algorithm based on quantum-behaved particle swarm algorithm has faster con- vergence speed with much better optimization performance compared with the mapping algorithm based on particle swarm algorithm. It also can effectively reduce the power consumption of mapping of three-dimensional network on chip.展开更多
With the development of magnetic manipulation technology based on magnetic nanoparticles(MNPs),scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells.The rapid in vitro co...With the development of magnetic manipulation technology based on magnetic nanoparticles(MNPs),scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells.The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues.Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs.Herein,safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h,and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled,layer by layer,in 5 min intervals.The in vivo transplantation results showed that in a stem cell microtissue with trisection architecture,the two separated human umbilical vein endothelial cell(HUVEC)layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels,which anastomosed with the host vasculature.The“hamburger”architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival.This study will contribute to the construction and application of structural and functional tissues or organs in the future.展开更多
The Biosecurity Law of the People’s Republic of China is the first basic,comprehensive,systematic,and overarching law of biosecurity in China,involving biosecurity risks,national biosecurity governance,and systems fo...The Biosecurity Law of the People’s Republic of China is the first basic,comprehensive,systematic,and overarching law of biosecurity in China,involving biosecurity risks,national biosecurity governance,and systems for minimizing the biosecurity threats.This article elaborates on the formulation,content,features,and significance of the law,and provides an outlook for its evolution.展开更多
文摘BACKGROUND Severe craniocerebral injury(STBI)is a critical physical trauma caused by a sudden external force acting on the head.The condition is complex and changeable,and disability and mortality rates are high.Although the life of STBI patients can be saved through treatment,the sequelae of consciousness,speech,cognitive impairment,stiffness,spasm,pain and abnormal behavior in the early rehabilitation stage can be a heavy burden to a family.In the past,routine nursing was often used to treat/manage STBI;however,problems,such as improper cooperation and untimely communication,reduced therapeutic effectiveness.AIM To investigate the effect of a proposed care bundle to optimize the first aid process and assess its effectiveness on the early rehabilitation nursing of patients with STBI.METHODS From January 2019 to December 2020,126 STBI patients were admitted to the emergency department of Chongqing Emergency Medical Center.These patients were retrospectively selected as the research participants in the current study.The study participants were then divided into a control group(61 cases)and a study group(65 cases).The control group was treated with routine nursing.The study group adopted the proposed care bundle.The National Institutes of Health Stroke Scale/Score and Glasgow Coma Scale(GCS)were used to evaluate neurological function before and after emergency treatment.After 3 mo of rehabilitation,experimental outcomes were assessed.These included the GCS,Barthel Index,complication rate,muscle strength grade and satisfaction.RESULTS There was no significant difference in gender,age,cause of injury and GCS between the two groups.After emergency,the National Institutes of Health Stroke Scale/Score of the study group(10.23±3.26)was lower than that of the control group(14.79±3.14).The GCS score of the study group(12.48±2.38)was higher than that of the control group(9.32±2.01).The arrival time of consultation in the study group was 20.56±19.12,and the retention time in the emergency room was 45.12±10.21,which were significantly shorter than those in the control group.After 3 mo of rehabilitation management,the GCS and Barthel Index of the study group were 14.56±3.75 and 58.14±12.14,respectively,which were significantly higher than those of the control group.The incidence of complications in the study group(15.38%)was significantly lower than that in the control group(32.79%).The proportion of muscle strength≥grade III in the study group(89.23%)was significantly higher than that in the control group(50.82%).The satisfaction of patients in the study group was significantly higher than that in the control group.CONCLUSION Care bundles are used to optimize the nursing process.During first-aid,care bundles can effectively improve the rescue effect and improve neurological function of STBI patients as well as shorten the treatment time.In early rehabilitation,they can effectively improve the consciousness of STBI patients,improve the activities of daily living,reduce the risk of complications,accelerate the recovery of muscle strength and improve their satisfaction.
基金supported by the National Key Research and Development Program of China(grant Nos.:2017YFD0301001 and 2016YFD0300403)the Shandong Province Mount Tai Industrial Talents Programthe National Natural Science Foundation of China(grant No.:31801295)。
文摘Drought at the grain filling stage of wheat will cause premature leaf senescence, thus leading to considerable loss of wheat yield. Therefore, this paper aims to establish a cultivation technology for strong drought resistance, delayed senescence, and yield improvement based on the analysis of hormones homeostasis obtained by applying chemical control substances. Experiments were conducted with two genotypes of wheat. Four water irrigation treatments were applied to impose the water deficit, including well-watered control treatment(WW), mild water deficit(MiWD), moderate water deficit(MoWD), and severe water deficit(SWD). Exogenous abscisic acid(ABA) was sprayed on the plants at the anthesis stage of the wheat. As a result, exogenous ABA reduced initial senescence rate(r0), total duration of chlorophyll(Chltotal), rapid senescence phase(Chlloss), and the accumulated temperature at an inflection point(M) but improved the persistence phase(Chlper) of flag leaves under all of the four treatments. However, exogenous ABA produced inconsistent effects on photoassimilate relocation and grain weight under different treatments. It produced positive regulatory effects on grain weight under WW, MiWD, and MoWD treatments. On the one hand, spraying ABA during the persistence phase of flag leaves reduced the ratios of zeatin to gibberellin(Z/GA3), spermine to spermidine(Spm/Spd), and salicylic acid to ABA(SA/ABA),which prolonged active photosynthesis by stimulating high level of proline(Pro) and increased the activities of antioxidant enzymes, such as superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX). Therefore, drought tolerance was enhanced, and more photosynthetic assimilates were accumulated. On the other hand, the rapid senescence phase and the transport rate of assimilates into grains were accelerated, resulting in higher grain weight, yield, and water use efficiency(WUE). However, under SWD treatment, exogenous ABA improved the ratio of SA/ABA, leading to low Pro content and low antioxidant enzyme activity of flag leaves in the rapid loss phase. Meanwhile,drought resistance declined and the transport duration of assimilates into grains was shortened, thus making photosynthetic assimilates redundant. Therefore, exogenous ABA can lead to the reduction in grain weight, yield, and WUE of wheat under SWD treatment.
基金Supported by National Natural Science Foundation of China(Grant Nos.51575147,51235003)Science Funds for the Young Innovative Talents of Harbin University of Science and Technology,China(Grant No.201507)
文摘To optimize milling force and machining accuracy quality in corner milling process, the changing law of milling force is revealed by Finite Element Method（FEM）. Based on DEFORM software a serial of 3D FEM models for corner milling process are devloped. Tool curved trajectory is achieved by establishing accurate relationship of tool location with milling time. Adaptive remeshing technique and iterative algorithm are adopted to ensure convergence of FEM model. Component force characteristics are revealed by analyzing FEM simulation results. It indicates that the milling force in Y direction becomes negative comparing with forces in X and Z direction. Magnitude of forces in three directions decreases with increase of spindle speed, while it increases with increase of milling feedrate. The simulation results for cutting force are in good agreement with those obtained from experiment. The FEM simulation model is first successfully established for corner milling process in this study, and the results provide a guide for optimizing cutting parameters in cutting process.
基金National Youth Science Fund Project(No.81900874)。
文摘Objective:To investigate the clinical study of Qiming granule combined with compound anisodine injection in the treatment of diabetic retinopathy.Methods:104 patients with nonproliferative diabetic retinopathy admitted to department of ophthalmology in Xishui Hospital Affiliated to Hubei University of science and technology from September 2016 to May 2018 were selected as the study subjects.According to the principle of treatment,they were divided into 52 cases in the control group and 52 cases in the treatment group,the clinical efficacy,blood sugar and lipid levels,hemorheological changes and adverse reactions were compared between the two groups.Results:The total effective rate difference between the treatment group(92.31%)and the control group(71.15%)was statistically significant(χ^(2)=7.792,P=0.005).After treatment,the two groups of fasting plasma glucose(FPG),hemoglobin A1c(HbA1c),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C),whole blood high-viscosity,whole blood cut viscosity,whole blood low-cut viscosity,plasma viscosity,number of microangioma and area of the hemorrhage were significantly lower than before treatment(P<0.05),and the levels of FPG,HbA1c,TC,LDL-C,whole blood highviscosity,whole blood cut viscosity,whole blood low-cut viscosity,plasma viscosity,number of microangioma and area of the hemorrhage in the treatment group were significantly lower than those in the control group(χ^(2)=0.122,P=0.727).Conclusions:Qiming granule combined with compound anisodine injection can effectively improve the clinical efficacy of patients with non-proliferative DR,and its mechanism may be related to improve blood sugar and blood lipid levels and correct blood rheology disorder,reduce microangioma and hemorrhage.It has clinical promotion significance.
基金Fundamental Research Funds for the Central Universities(2042022kf1206)National Natural Science Foundation of China(82271010).
文摘Periodontitis is an inflammatory disease caused by bacterial biofilms,which leads to the destruction of periodontal tissue.Current treatments,such as mechanical cleaning and antibiotics,struggle to effectively address the persistent biofilms,inflammation,and tissue damage.A new approach involves developing a Janus nanomotor(J-CeM@Au)by coating cerium dioxide-doped mesoporous silica(CeM)with gold nanoparticles(AuNPs).This nanomotor exhibits thermophoretic motion when exposed to near-infrared(NIR)laser light due to the temperature gradient produced by the photothermal effects of asymmetrically distributed AuNPs.The NIR laser provides the energy for propulsion and activates the nanomotor's antibacterial properties,allowing it to penetrate biofilms and kill bacteria.Additionally,the nanomotor's ability to scavenge reactive oxygen species(ROS)can modulate the immune response and create a regenerative environment,promoting the healing of periodontal tissue.Overall,this multifunctional nanomotor offers a promising new approach for treating periodontitis by simultaneously addressing biofilm management and immune modulation with autonomous movement.
基金supported by the National Key Research and Development Program of China(Grant no.2020YFA0908603).
文摘The sharing of pathogens is of great significance for the continuous and comprehensive testing and monitoring of viral samples,vaccine and drug development,and the study of drug resistance and mutability of viral samples.This paper reviews the current legal framework in the field of pathogen sharing,analyzes existing issues,and proposes recommendations to improve the legal framework of pathogen sharing.
基金supported by the National Natural Science Foundation of China(No.82001106,81970918,81901043).
文摘Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.
基金This work was financially supported by National Natural Science Foundation of China(81901043,81970918,and 82001106)J.Yu’s research conducted at the UBC Faculty of Dentistry was supported by China Scholarship Council(202006275049).
文摘As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed dentinal tubules provide channels for irritants and bacterial invasion,leading to dentin hypersensitivity and even pulp inflammation.Cariogenic bacterial adhesion and biofilm formation on dentin are responsible for tooth demineralization and caries.It remains a clinical challenge to achieve the integration of tubule occlusion,collagen mineralization,and antibiofilm functions for managing exposed dentin.To address this issue,an epigallocatechin-3-gallate(EGCG)and poly(allylamine)-stabilized amorphous calcium phosphate(PAH-ACP)co-delivery hollow mesoporous silica(HMS)nanosystem(E/PA@HMS)was herein developed.The application of E/PA@HMS effectively occluded the dentinal tubules with acid-and abrasion-resistant stability and inhibited the biofilm formation of Streptococcus mutans.Intrafibrillar mineralization of collagen fibrils and remineralization of demineralized dentin were induced by E/PA@HMS.The odontogenic differentiation and mineralization of dental pulp cells with high biocompatibility were also promoted.Animal experiments showed that E/PA@HMS durably sealed the tubules and inhibited biofilm growth up to 14 days.Thus,the development of the E/PA@HMS nanosystem provides promising benefits for protecting exposed dentin through the coordinated manipulation of dentin caries and hypersensitivity.
文摘The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,3 days)in Fig.5c were mistakenly used during figure assembly.We tracked down the original data obtained in June 2022,and have replaced Fig.5 with the correct image as follows.The authors would like to apologise for any inconvenience caused and state that the correction does not change the scientific conclusions of the article in any way.
文摘Mapping of three-dimensional network on chip is a key problem in the research of three-dimensional network on chip. The quality of the mapping algorithm used di- rectly affects the communication efficiency between IP cores and plays an important role in the optimization of power consumption and throughput of the whole chip. In this paper, ba- sic concepts and related work of three-dimensional network on chip are introduced. Quantum-behaved particle swarm op- timization algorithm is applied to the mapping problem of three-dimensional network on chip for the first time. Sim- ulation results show that the mapping algorithm based on quantum-behaved particle swarm algorithm has faster con- vergence speed with much better optimization performance compared with the mapping algorithm based on particle swarm algorithm. It also can effectively reduce the power consumption of mapping of three-dimensional network on chip.
基金the National Natural Science Foundation of China(Grant Nos.81921002,81620108006,31700848,82001009)the National Key Research and Development Program of China(2016YFC1102900)。
文摘With the development of magnetic manipulation technology based on magnetic nanoparticles(MNPs),scaffold-free microtissues can be constructed utilizing the magnetic attraction of MNP-labeled cells.The rapid in vitro construction and in vivo vascularization of microtissues with complex hierarchical architectures are of great importance to the viability and function of stem cell microtissues.Endothelial cells are indispensable for the formation of blood vessels and can be used in the prevascularization of engineered tissue constructs.Herein,safe and rapid magnetic labeling of cells was achieved by incubation with MNPs for 1 h,and ultrathick scaffold-free microtissues with different sophisticated architectures were rapidly assembled,layer by layer,in 5 min intervals.The in vivo transplantation results showed that in a stem cell microtissue with trisection architecture,the two separated human umbilical vein endothelial cell(HUVEC)layers would spontaneously extend to the stem cell layers and connect with each other to form a spatial network of functional blood vessels,which anastomosed with the host vasculature.The“hamburger”architecture of stem cell microtissues with separated HUVEC layers could promote vascularization and stem cell survival.This study will contribute to the construction and application of structural and functional tissues or organs in the future.
文摘The Biosecurity Law of the People’s Republic of China is the first basic,comprehensive,systematic,and overarching law of biosecurity in China,involving biosecurity risks,national biosecurity governance,and systems for minimizing the biosecurity threats.This article elaborates on the formulation,content,features,and significance of the law,and provides an outlook for its evolution.
