An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colore...An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide.Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.展开更多
基金supported by the Open Project of the State Key Laboratory of Veterinary Etiological Biology, China (SKLVEB 2013 KFKT 0015)
文摘An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide.Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.
文摘目的 探讨新生儿行为神经测定(NBNA)评分联合血清胆红素总量/白蛋白比值(B/A)预测新生儿高胆红素血症并发急性胆红素脑病的临床价值。方法 收集2020年5月—2021年6月杭州市妇产科医院收治的患儿62例为观察组,另选取在院进行健康体检的婴儿62例为对照组。检测两组NBNA评分、B/A水平,分析NBNA评分、B/A在高胆红素血症及其并发病情的表达水平以及对高胆红素血症以及并发症状的诊断价值。结果 观察组B/A表达水平高于对照组(8.51±2.03 vs. 5.69±1.35),NBNA评分表达水平低于对照组(26.88±4.23 vs. 35.33±5.61),差异有统计学意义(P<0.05)。logistic回归分析显示,B/A水平表达上升,NBNA评分表达水平下降是患有高胆红素血症以及并发不良病症患儿的重要影响机制。ROC曲线显示,两项联合对患有高胆红素血症及并发不良病症患儿的诊断价值高于NBNA评分、B/A单项诊断[AUC(95%CI):0.944(0.896~0.991)vs.0.823(0.749~0.897)vs.0.782(0.699~0.866)],差异有统计学意义(P<0.05)。结论 对于研究患有不良病症患儿情况变化,可检测出B/A水平表达上升,NBNA评分表达下降,能够有效地提前对患儿病情进行预测,减少不良反应的发生。
