The literature reports mainly focus on 2023-2024,and the research hotspots are mostly productivity and new quality productivity.After analysis,research in the field of fisheries is still in its early stages.Among them...The literature reports mainly focus on 2023-2024,and the research hotspots are mostly productivity and new quality productivity.After analysis,research in the field of fisheries is still in its early stages.Among them,the facilities,intelligence,and"Blue granary"of marine fisheries need to be further strengthened to provide technical support for achieving the goal of"new quality productivity"in the fishing industry in the future.展开更多
According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Const...According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Construction"emphasizes the development of circular aquaculture,active expansion of green aquaculture space in facility fisheries,multi-channel development of food sources,comprehensive improvement of the quality,efficiency,and competitiveness of facility fisheries,ensuring stable and safe supply of aquatic products,and better meeting people's needs for high-quality aquatic products and beautiful aquatic ecological environment.Since the implementation of the 14th Five Year Plan for fisheries,facility fisheries have made significant progress.In order to thoroughly implement General Secretary Xi Jinping's important discourse on new quality productivity,conduct in-depth research on the current situation and existing problems of the fishery industry,and put forward suggestions to meet the requirements of high-quality development of the fishery industry in the new era.Therefore,it is crucial to continue practicing the"revitalization of aquatic seed industry"and"big food concept",take multiple measures to"demand food from rivers,lakes,and seas",and expand aquaculture space.Through research,it has been found that research in the field of facility based fisheries is still in its developmental stage.Further exploration is needed on the current status,breeding models,and experimental demonstrations of land-based facility based fisheries,in order to provide a basis for fisheries regulatory authorities to formulate the"15th Five Year Plan"for facility based fisheries.展开更多
[ Objective] To investigate the mechanisms involved in the Up-regulatory effects of 17β-estrodiol on β-defensin-2 （SBD-2） in epithelial cells of ovine oviduct. [ Methods] Epithelial cells of ovine oviduct were iso...[ Objective] To investigate the mechanisms involved in the Up-regulatory effects of 17β-estrodiol on β-defensin-2 （SBD-2） in epithelial cells of ovine oviduct. [ Methods] Epithelial cells of ovine oviduct were isolated and cultured; and then the cultured cells at secondary generation were divided into 17β-estradiol （E2, 10^-8 tool/L） group, estrogen nuclear receptor antagonist ICI182780 （10^-7 tool/L） group, PKA antagonist KT-5720 （1 μmol/L） group, PKC antagonist H- 7（50 μmol/L） group, nuclear factor kappa B antagonist PDTC（50μmol/L） group and the blank control group （ Control ）. Firstly, different antagonists were added into corresponding antagonist groups in order to interfere the epithelial ceils of ovine oviduct for 1 h. Then, 17β-estradiol （ 10^-8 mol/L） was added into each antagonist group and E2 group for cultivation for 6 h. Finally, real-time fluorescent quantitative RT-PCR was used to detect the changes of SBD-2 mRNA expression. [ Results] 10^-8 mol/L 17β-estrodiol had significantly Up-regulatory effects on the expression of SBD-2 mRNA （P 〈 0. 05 ）. Estrogen nuclear receptor antagonist ICI182780, NF-κB antagonist PDTC and PKC antagonist H-7 could all block the Up-regnlatory effects on SBD-2. But PKA antagonist KT-5720 showed no significant effects on the Up-regulation of SBD-2 mRNA expression induced by 17β-estrodiol. [ Conclusions] SBD-2 mRNA expression induced by 17β-estrodiol in epithelial cells of ovine oviduct was mediated by estrogen nuclear receptor ICI182780, NF-κB and PKC pathways. However, PKA pathway might not participate in the Up-regulation of SBD-2 mRNA expression.展开更多
基金supported by the Technical system of national bulk freshwater fish industry(No.CARS-45-33)National bulk freshwater fish industry technology system Tianjin freshwater aquaculture industry technology system innovation team(No.ITTFRS2024000)+2 种基金Tianjin Science and Technology Plan Project(No.23YDTPJC00420)Central Guiding Local Science and Technology Development Funds(No.24ZYCGSN00250)Opening Foundation of Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria(No.202007 and 202105).
文摘The literature reports mainly focus on 2023-2024,and the research hotspots are mostly productivity and new quality productivity.After analysis,research in the field of fisheries is still in its early stages.Among them,the facilities,intelligence,and"Blue granary"of marine fisheries need to be further strengthened to provide technical support for achieving the goal of"new quality productivity"in the fishing industry in the future.
基金supported by the National Bulk Freshwater Fish Industry Technology System(CARS-45-33)Tianjin Freshwater Aquaculture Industry Technology System Innovation Team(ITTFRS20211000-005/002)+2 种基金Tianjin Science and Technology Plan Project(23YDTPJC00420)Central Guiding Local Science and Technology Development Fund(24ZYCGSN00250)Open Fund of Key Laboratory of Environmental Standards and Environmental Pollution of the Ministry of Education(202007,202105).
文摘According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Construction"emphasizes the development of circular aquaculture,active expansion of green aquaculture space in facility fisheries,multi-channel development of food sources,comprehensive improvement of the quality,efficiency,and competitiveness of facility fisheries,ensuring stable and safe supply of aquatic products,and better meeting people's needs for high-quality aquatic products and beautiful aquatic ecological environment.Since the implementation of the 14th Five Year Plan for fisheries,facility fisheries have made significant progress.In order to thoroughly implement General Secretary Xi Jinping's important discourse on new quality productivity,conduct in-depth research on the current situation and existing problems of the fishery industry,and put forward suggestions to meet the requirements of high-quality development of the fishery industry in the new era.Therefore,it is crucial to continue practicing the"revitalization of aquatic seed industry"and"big food concept",take multiple measures to"demand food from rivers,lakes,and seas",and expand aquaculture space.Through research,it has been found that research in the field of facility based fisheries is still in its developmental stage.Further exploration is needed on the current status,breeding models,and experimental demonstrations of land-based facility based fisheries,in order to provide a basis for fisheries regulatory authorities to formulate the"15th Five Year Plan"for facility based fisheries.
基金Supported by the National Natural Science Foundation of China(31060328)the Natural Science Foundation of Inner Mongolia(No.2014BS0801)+1 种基金the Doctor's Start-up Fund in Inner Mongolia Medical University(NY2011BQ003)the Youth Entrepreneurship Foundation of Inner Mongolia Medical University(NY2010QN003)
文摘[ Objective] To investigate the mechanisms involved in the Up-regulatory effects of 17β-estrodiol on β-defensin-2 （SBD-2） in epithelial cells of ovine oviduct. [ Methods] Epithelial cells of ovine oviduct were isolated and cultured; and then the cultured cells at secondary generation were divided into 17β-estradiol （E2, 10^-8 tool/L） group, estrogen nuclear receptor antagonist ICI182780 （10^-7 tool/L） group, PKA antagonist KT-5720 （1 μmol/L） group, PKC antagonist H- 7（50 μmol/L） group, nuclear factor kappa B antagonist PDTC（50μmol/L） group and the blank control group （ Control ）. Firstly, different antagonists were added into corresponding antagonist groups in order to interfere the epithelial ceils of ovine oviduct for 1 h. Then, 17β-estradiol （ 10^-8 mol/L） was added into each antagonist group and E2 group for cultivation for 6 h. Finally, real-time fluorescent quantitative RT-PCR was used to detect the changes of SBD-2 mRNA expression. [ Results] 10^-8 mol/L 17β-estrodiol had significantly Up-regulatory effects on the expression of SBD-2 mRNA （P 〈 0. 05 ）. Estrogen nuclear receptor antagonist ICI182780, NF-κB antagonist PDTC and PKC antagonist H-7 could all block the Up-regnlatory effects on SBD-2. But PKA antagonist KT-5720 showed no significant effects on the Up-regulation of SBD-2 mRNA expression induced by 17β-estrodiol. [ Conclusions] SBD-2 mRNA expression induced by 17β-estrodiol in epithelial cells of ovine oviduct was mediated by estrogen nuclear receptor ICI182780, NF-κB and PKC pathways. However, PKA pathway might not participate in the Up-regulation of SBD-2 mRNA expression.
