BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a deadly malignancy with limited treatment options.Deubiquitinases(DUBs)have been confirmed to play a crucial role in the development of malignant tumors.JOSD2 is ...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a deadly malignancy with limited treatment options.Deubiquitinases(DUBs)have been confirmed to play a crucial role in the development of malignant tumors.JOSD2 is a DUB involved in con-trolling protein deubiquitination and influencing critical cellular processes in cancer.AIM To investigate the impact of JOSD2 on the progression of ESCC.METHODS Bioinformatic analyses were employed to explore the expression,prognosis,and enriched pathways associated with JOSD2 in ESCC.Lentiviral transduction was utilized to manipulate JOSD2 expression in ESCC cell lines(KYSE30 and RESULTS )Preliminary research indicated that JOSD2 was highly expressed in ESCC tissues,which was associated with poor prognosis.Further analysis demonstrated that JOSD2 was upregulated in ESCC cell lines compared to normal esophageal cells.JOSD2 knockdown inhibited ESCC cell activity,including proliferation and colony-forming ability.Moreover,JOSD2 knockdown decreased the drug resistance and migration of ESCC cells,while JOSD2 overexpression enhanced these phenotypes.In vivo xenograft assays further confirmed that JOSD2 promoted tumor proliferation and drug resistance in ESCC.Mechanistically,JOSD2 appears to activate the MAPK/ERK and PI3K/AKT signaling pathways.Mass spectrometry was used to identify crucial substrate proteins that interact with JOSD2,which identified the four primary proteins that bind to JOSD2,namely USP47,IGKV2D-29,HSP90AB1,and PRMT5.CONCLUSION JOSD2 plays a crucial role in enhancing the proliferation,migration,and drug resistance of ESCC,suggesting that JOSD2 is a potential therapeutic target in ESCC.展开更多
Active Magnetic Bearing(AMB) is a kind of electromagnetic support that makes the rotor movement frictionless and can suppress rotor vibration by controlling the magnetic force. The most common approach to restrain the...Active Magnetic Bearing(AMB) is a kind of electromagnetic support that makes the rotor movement frictionless and can suppress rotor vibration by controlling the magnetic force. The most common approach to restrain the rotor vibration in AMBs is to adopt a notch filter or adaptive filter in the AMB controller. However, these methods cannot obtain the precise amplitude and phase of the compensation current. Thus, they are not so effective in terms of suppressing the vibrations of the fundamental and other harmonic orders over the whole speed range. To improve the vibration suppression performance of AMBs,an adaptive filter based on Least Mean Square(LMS) is applied to extract the vibration signals from the rotor displacement signal. An Iterative Search Algorithm(ISA) is proposed in this paper to obtain the corresponding relationship between the compensation current and vibration signals. The ISA is responsible for searching the compensating amplitude and shifting phase online for the LMS filter, enabling the AMB controller to generate the corresponding compensation force for vibration suppression. The results of ISA are recorded to suppress vibration using the Look-Up Table(LUT) in variable speed range. Comprehensive simulations and experimental validations are carried out in fixed and variable speed range, and the results demonstrate that by employing the ISA, vibrations of the fundamental and other harmonic orders are suppressed effectively.展开更多
Symptoms that are multidimensional and concurrent should be assessed from different dimensions and managed together. Few studies have evaluated concurrent and multidimensional symptoms in patients with stroke. Most st...Symptoms that are multidimensional and concurrent should be assessed from different dimensions and managed together. Few studies have evaluated concurrent and multidimensional symptoms in patients with stroke. Most studies of stroke focused on dysfunctions and complications. We hypothesize that patients with stroke have a heavy symptom burden within 1 year. This study aimed to describe multidimensional and concurrent symptoms within 1 year after stroke. This study recruited 230 patients with stroke from the Rehabilitation Department of Xuhui District Center Hospital of Shanghai and the Shanghai Sunshine Rehabilitation Center in China from March to September 2017. The patients＇ multidimensional symptom experience and symptom burden were analyzed using a self-made structured questionnaire and the influential factors for symptom burden were identified. The mean number of symptoms in patients with stroke was 11.7 ± 3.5. More than two thirds of the participants suffered from at least 10 co-occurring symptoms. Unilateral limb weakness had the highest prevalence and frequency. Participation restriction had the highest symptom dimensions of severity and distress. Lack of self-care ability（severity）, memory deterioration（frequency）, imbalance of body（distress）, moodiness（distress）, being unable to move limbs at will（distress）, shoulder pain（distress）, and slower response（frequency） were independent factors of the total symptom burden score. These findings can provide essential information for efficient symptom management of patients with stroke.展开更多
Background: Polysaccharides extracted from the medicinal herbs are known to possess antitumor effects. Although there is a significant number of evidences implicated on the beneficial effects of the ginseng leaves gin...Background: Polysaccharides extracted from the medicinal herbs are known to possess antitumor effects. Although there is a significant number of evidences implicated on the beneficial effects of the ginseng leaves ginsenoside with diverse associated mechanisms, reports on the anticancer by the ginseng leaves crude polysaccharides (GLCP) are not sufficient. Experiments were carried out to unravel the anticancer effects of GLCP. Methods: Cells were treated with GLCP (0.5 - 2 mg/ml) for 48h. MTT method was used to detect the cell viability. Western blot and flow cytometry were used to detect apoptotic rate. Western blot and acridine orange staining were used to detect the cell autophagy. Results: Compared with the normal human liver cell (Chang liver), GLCP (1.5 - 2 mg/ml) significantly reduction cell viability, promote apoptosis-related proteins expression, promote cell apoptosis and autophagy in SMMC-7721 cells. But caveolin-1 gene silencing could inhibit the anticancer effect of GLCP. Conclusions: These data suggest that GLCP promote autophagy and apoptosis in human hepatoma cell SMMC-7721. We speculate that its mechanism may be associated with the caveolin-1 which is an essential structural molecule of caveolae. Although the effect of GLCP inhibited of liver cancer is not very strong, we are more interested in the GLCP which plays a tumor suppressor role in health care.展开更多
This paper studies the environmental effects of technical change using a spatial model with panel data from 284 prefecture-cities over 2004-2015 in China.We find that the effects of technical change vary across differ...This paper studies the environmental effects of technical change using a spatial model with panel data from 284 prefecture-cities over 2004-2015 in China.We find that the effects of technical change vary across different dimensions of technical change and different pollution indicators.Furthermore,we also provide robust evidence for the existence of the spatial effects of technical change on environmental pollution across cities.First,indigenous technical change displays three patterns of effects on the four pollutants:a positive effect on wastewater,a negative effect on PM_(2.5)concentrations,and an inverted U-shaped relationship with SO_(2)and soot emissions.The spatial effect of indigenous technical change promotes cleaner industrial productions(fewer emissions of SO_(2),soot and wastewater)but higher PM_(2.5)concentrations.Second,technology transfers from foreign direct investment are associated with less pollution except for wastewater,and their spatial effects are unanimously negative on all pollutants.Finally,absorptive capacity can also promote cleaner industrial production,but its spatial effects can do otherwise.Accordingly,the government should take the spatial spillover effects of technical change into account when implementing specific policies,pin down specific pollutants to make full use of the pollution-reducing effects of technical change,and improve the absorptive capacity of domestic firms.展开更多
Mutations induced from tissue culture are easily to be separated,which might be propagated in the same medium,especially for the color-leaf ornamental grass,Oxalis triaggularis purpurea.Mutations of the ornamental tra...Mutations induced from tissue culture are easily to be separated,which might be propagated in the same medium,especially for the color-leaf ornamental grass,Oxalis triaggularis purpurea.Mutations of the ornamental traits in the tissue culture bottle could be investigated easily.The 35 d regeneration system group showed the lowest adventitious bud number and adventitious root number among 4 inoculation dates by 50 Gy dose of ^(60)Coγ radiation.More studies were carried out based on the 35d differentiation state of O.triangularis purpurea regeneration system.The 35 d regeneration system was then irradiated by 10,25 and 50 Gy doses of ^(60)Coγ rays.Numbers of adventitious buds and roots induced from the regeneration system were cut down with the increment of radiation doses.Seedling length was not distinctly reduced at the absorbed doses of 10 and 25 Gy,but reduced distinctly under 50 Gy of ^(60)Coγ irradiation.The optimal irradiation dose for 35 d O.triangularis regeneration system survival and mutation induction was approximately 25 Gy.The M_2 phenotypic mutation rate was 2.9%,especially,and the leaf number mutation accounted for 76%of the total mutation.The phenotypic mutations,especially in the 10 Gy group,on 0.1 m M Vc containing MS medium were recovered,which indicated that ROS plays a key role in the phenotypic mutation induced by ^(60)Coγ -rays.展开更多
The nuclear transcription factors κB (NF-κB) is widely existing in various kinds of cell types in the nervous system and plays an important role in neuron apoptosis and neurodegenerative diseases. Estrogen receptor ...The nuclear transcription factors κB (NF-κB) is widely existing in various kinds of cell types in the nervous system and plays an important role in neuron apoptosis and neurodegenerative diseases. Estrogen receptor alpha 36 (ER-α36), is a novel variant of ERα (as known ER-α66) which can transduce both estrogenand antiestrogen-dependent activation of MAPK signal pathway and stimulate cell growth. Here, we aimed to detect the effect of ER-α36 gene silencing on the expression of NF-κB in normal cultured PC12 cells and to provide an experimental foundation for understanding the function of ER-α36 innerve cells. PC12 cells with ER-α36 expression knocked down by the shRNA method. Then Western blot and immunocytochemical staining were performed to detect the expression and translocation of NF-κB after transfection. The results showed that NF-κB expression was significantly higher comparing with the control group after transfection (P 0.01). Also, NF-κB subunit entered nuclear after transfection;Immunofluorescence staining and immunocytochemical staining of PC12 cells demonstrated that ER-α36 was expressed mainly on the plasma membrane and on the cell nucleus membrane. These data indicate that ER-α36 gene silencing can increase the expression of NF-κB and promote its nuclear translocation in PC12 cells.展开更多
Synchronous reluctance machine(SynRM)can be perceived as a special type of permanent magnet synchronous machine(PMSM),and shares similar control method,i.e.the sine-wave current drive with sinusoidal phase currents.In...Synchronous reluctance machine(SynRM)can be perceived as a special type of permanent magnet synchronous machine(PMSM),and shares similar control method,i.e.the sine-wave current drive with sinusoidal phase currents.In this paper,square-wave drive,which is employed for permanent magnet brushless(BLDC)motors,is employed for the SynRM,in order to economically reduce the cost of rotor position sensor.It is revealed that the torque density and efficiency are slightly sacrificed,whereas torque ripple is deteriorated,proving the SynRM with square-wave drive still promising for the cost-sensitive application if torque ripple is not considered as a critical issue.To further investigate the additional pulsating torque under square-wave drive,mathematical model based on a-b-c phase inductance and d-q axis inductance are established,together with the time-stepping FE calculated currents.It is concluded that the harmonics in the currents tend to cause non-sinusoidal variation of the magnetic reluctance,which can be represented as additional inductance harmonics.The harmonics of the current and inductance interact with each other,thus undesirable torque ripple components are produced.展开更多
Cardiac biological pacing(BP)is one of the future directions for bradyarrhythmias intervention.Currently,cardiac pacemaker cells(PCs)used for cardiac BP are mainly derived from pluripotent stem cells(PSCs).However,the...Cardiac biological pacing(BP)is one of the future directions for bradyarrhythmias intervention.Currently,cardiac pacemaker cells(PCs)used for cardiac BP are mainly derived from pluripotent stem cells(PSCs).However,the production of high-quality cardiac PCs from PSCs remains a challenge.Here,we developed a cardiac PC differentiation strategy by adopting dual PC markers and simulating the developmental route of PCs.First,two PC markers,Shox2 and Hcn4,were selected to establish Shox2:EGFP;Hcn4:mCherry mouse PSC reporter line.Then,by stepwise guiding naïve PSCs to cardiac PCs following naïve to formative pluripotency transition and manipulating signaling pathways during cardiac PCs differentiation,we designed the FSK method that increased the yield of SHOX2^(+);HCN4^(+)cells with typical PC characteristics,which was 12 and 42 folds higher than that of the embryoid body(EB)and the monolayer M10 methods respectively.In addition,the in vitro cardiac PCs differentiation trajectory was mapped by single-cell RNA sequencing(scRNA-seq),which resembled in vivo PCs development,and ZFP503 was verified as a key regulator of cardiac PCs differentiation.These PSC-derived cardiac PCs have the potential to drive advances in cardiac BP technology,help with the understanding of PCs(patho)physiology,and benefit drug discovery for PC-related diseases as well.展开更多
Histone modifications play crucial roles in the pathogenesis of myocardial ischaemia/reperfusion(I/R)injury.However,a genome-wide map of histone modifications and the underlying epigenetic signatures in myocardial I/R...Histone modifications play crucial roles in the pathogenesis of myocardial ischaemia/reperfusion(I/R)injury.However,a genome-wide map of histone modifications and the underlying epigenetic signatures in myocardial I/R injury have not been established.Here,we integrated transcriptome and epigenome of histone modifications to characterize epigenetic signatures after I/R injury.Disease-specific histone mark alterations were mainly found in H3K27me3-,H3K27ac-,and H3K4me1-marked regions 24 and 48 h after I/R.Genes differentially modified by H3K27ac,H3K4me1 and H3K27me3 were involved in immune response,heart conduction or contraction,cytoskeleton,and angiogenesis.H3K27me3 and its methyltransferase polycomb repressor complex 2(PRC2)were upregulated in myocardial tissues after I/R.Upon selective inhibition of EZH2(the catalytic core of PRC2),the mice manifest improved cardiac function,enhanced angiogenesis,and reduced fibrosis.Further investigations confirmed that EZH2 inhibition regulated H3K27me3 modification of multiple pro-angiogenic genes and ultimately enhanced angiogenic properties in vivo and in vitro.This study delineates a landscape of histone modifications in myocardial I/R injury,and identifies H3K27me3 as a key epigenetic modifier in I/R process.The inhibition of H3K27me3 and its methyltransferase might be a potential strategy for myocardial I/R injury intervention.展开更多
B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipient...B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipients was investigated, its potential significance was analyzed and peripheral blood of follow-up kidney transplant recipients was studied. Flow cytometric assay results showed that, cell-surface BAFF was significantly highly expressed on peripheral CD3＋ T lymphocytes in 〉 5 yrs group of kidney transplant recipients, compared with other groups （p 〈 0.05）. BAFF expression could be found on CD4＋ T cells and CD8＋ T cells. The BAFF mRNA levels in peripheral mononuclear cells were consistent with the protein levels. However, serum soluble BAFF levels were inter- individually different in each group. Stratified by renal function, it was found that cell-surface BAFF levels were significantly higher in those with abnormal renal function, compared with recipients with normal renal function （p 〈 0.05）. ELISA assay results showed that expression levels of cell surface BAFF were significantly correlated with anti-HLA I ＆ II antibodies. These results indicate that BAFF may be involved in the development of graft-loss and influences the long-time outcome of kidney allografl, likely mediated by interfering with immune response. Cellular ＆ Molecular Immunology. 2008;5（6）：465-470.展开更多
Mitogen-activated protein kinases(MAPK) and nuclear factor kappa B(NF-κB) pathways are considered to be two crucial intracellular signaling cascades in pro-inflammatory responses.In this study,we reported the coding ...Mitogen-activated protein kinases(MAPK) and nuclear factor kappa B(NF-κB) pathways are considered to be two crucial intracellular signaling cascades in pro-inflammatory responses.In this study,we reported the coding sequences(CDS) of MAPKp38 and NF-κBp65 from yellow catfish.We also investigated the gene structure,expression patterns and functional role in yellow catfish.The CDS of MAPKp38 is1,086 bp encoding 361 amino acids(AA).The MAPKp38 protein has a long highly conserved serine/threonine protein kinases catalytic domain.The NF-κBp65 CDS is 1,794 bp,and the gene encodes 597 AA,with a Rel homology domain(RHD) which consists of a RHD-DNA-binding domain and an Ig-like,plexins,transcription factors(IPT) domain.Moreover,MAPKp38 and NF-κBp65 protein of bony fish and other vertebrates have a single clade.Quantitative real-time PCR analysis revealed the presence of the MAPKp38 and NF-κBp65 transcript in 12 tissues of healthy yellow catfish.The highest expression levels of MAPKp38 and NF-κBp65 were detected in the heart and liver,respectively.Upon stimulation with an intraperitoneal injection of lipopolysaccharide(LPS),the expression levels of MAPKp38 and NF-κBp65 were up-regulated in the intestine.These results indicated that MAPKp38 and NF-κBp65 play important roles in mediating the response protection against LPS in yellow catfish.展开更多
At present, the development of renewable energy relies mainly on government support. The government invests in a considerable number of projects to improve public welfare and to assist in poverty relief If China is to...At present, the development of renewable energy relies mainly on government support. The government invests in a considerable number of projects to improve public welfare and to assist in poverty relief If China is to replace fossil fuels on a large scale with renewable energy sources, the production costs and prices of renewable energy must be brought down. All countries are facing the challenge of moving to a more secure and low-carbon energy system without weakening economic and social development. In this regard, China is facing an even greater challenge in terms of economic cost, as cheap coal remains the main energy form. Technical innovation and industrialization in the area of renewable energy is an important means of lowering cost. China is in for a period of high-speed development of its economy and the rising demand for energy is irreversible. If the technical progress and development speed of renewable energy lags behind the growth in demand, it will be difficult to realize the improvement of its energy structure.展开更多
In this paper, we develop models to determine operational and financial decisions of a supply chain under the condition that the retailer faces a financial constraint and the manufacturer can offer trade credit to ass...In this paper, we develop models to determine operational and financial decisions of a supply chain under the condition that the retailer faces a financial constraint and the manufacturer can offer trade credit to assist the retailer. We first study the case where the retailer is risk-neutral, and derive the optimal ordering and financial decisions. Then, the case where the retailer is risk-averse （downside risk） is studied and-the effects of the risk on the retailer and manufacturer＇s operational and financial decisions are discussed. Finally, numerical examples are provided to conduct managerial analysis.展开更多
In this study, diamond films were synthesized on silicon substrates by microwave plasma enhanced chemical vapor deposition（CVD） over a wide range of experimental parameters. The effects of the microwave power,CH;/H;...In this study, diamond films were synthesized on silicon substrates by microwave plasma enhanced chemical vapor deposition（CVD） over a wide range of experimental parameters. The effects of the microwave power,CH;/H;ratio and gas pressure on the morphology, growth rate, composition, and quality of diamond films were investigated by means of scanning electron microscope（SEM）, X-ray diffraction（XRD）, Raman spectroscopy and X-ray photoelectron spectroscopy（XPS）. A rise of microwave power can lead to an increasing pyrolysis of hydrogen and methane, so that the microcrystalline diamond film could be synthesized at low CH;/H;levels. Gas pressure has similar effect in changing the morphology of diamond films, and high gas pressure also results in dramatically increased grain size. However,diamond film is deteriorated at high CH;/H;ratio due to the abundant graphite content including in the films. Under an extreme condition of high microwave power of 10 kW and high CH;concentration, a hybrid film composed of diamond/graphite was successfully formed in the absence of N;or Ar,which is different from other reports. This composite structure has an excellent measured sheet resistance of 10-100 Ω/Sqr. which allows it to be utilized as field electron emitter. The diamond/graphite hybrid nanostructure displays excellent electron field emission（EFE） properties with a low turn-on field of 2.17 V/μm and β= 3160, therefore it could be a promising alternative in field emission applications.展开更多
Tremendous demands for highly sensitive and stable seawater salinometers have motivated intensive research on advanced electrode materials.Boron-doped diamond(BDD)is attractive in terms of its high mechanical stabilit...Tremendous demands for highly sensitive and stable seawater salinometers have motivated intensive research on advanced electrode materials.Boron-doped diamond(BDD)is attractive in terms of its high mechanical stability and chemical inertness,but is usually hindered by its low double-layer capacitance(C_(dl))for seawater salinity detection.Here,inspired by the principle of oxygen-terminated BDD electrode endowing higher C_(dl)than hydrogen-terminated surface,we introduce the oxygen terminated surface by oxygen plasma or reactive ion etch(RIE),and the fabricated oxygen terminated BDD electrodes demonstrate high sensitivity and long-term stability in seawater salinity detection comparing with the hydrogen terminated BDD electrodes.Significantly,the as-fabricated O-BDD-RIE electrodes not only show remarkable enhanced response even better than the commercial platinum black electrodes but also display long-time stability which is weekly verified by continuous monitor for 90 days.The outstanding performance of the oxygen terminated BDD electrodes can be ascribed to the enhancement of C-O surface functional group on C_(dl).In addition,a comprehensive analysis of effective electroactive surface area(EASA)and C_(dl)proves that the surface oxygen is the major factor for the improved C_(dl).In summary,the excellent oxygen terminated BDD electrodes promise potential application in seawater salinity detection.展开更多
基金Supported by Tianjin Key Medical Discipline(Specialty)Construction Project,No.TJYXZDXK-009ATianjin Medical University Cancer Hospital National Natural Science Foundation Cultivation Program,No.220108+3 种基金National Natural Science Foundation of China,No.82373134Science and Technology Development Fund of Tianjin Education Commission for Higher Education,No.2022KJ228Chinese Anti-Cancer Association-Heng Rui Anti-angiogenesis Targeted Tumor Research Fund,No.2021001045and Scientific Research Translational Foundation of Wenzhou Safety(Emergency)Institute of Tianjin University,No.TJUWYY2022025.
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a deadly malignancy with limited treatment options.Deubiquitinases(DUBs)have been confirmed to play a crucial role in the development of malignant tumors.JOSD2 is a DUB involved in con-trolling protein deubiquitination and influencing critical cellular processes in cancer.AIM To investigate the impact of JOSD2 on the progression of ESCC.METHODS Bioinformatic analyses were employed to explore the expression,prognosis,and enriched pathways associated with JOSD2 in ESCC.Lentiviral transduction was utilized to manipulate JOSD2 expression in ESCC cell lines(KYSE30 and RESULTS )Preliminary research indicated that JOSD2 was highly expressed in ESCC tissues,which was associated with poor prognosis.Further analysis demonstrated that JOSD2 was upregulated in ESCC cell lines compared to normal esophageal cells.JOSD2 knockdown inhibited ESCC cell activity,including proliferation and colony-forming ability.Moreover,JOSD2 knockdown decreased the drug resistance and migration of ESCC cells,while JOSD2 overexpression enhanced these phenotypes.In vivo xenograft assays further confirmed that JOSD2 promoted tumor proliferation and drug resistance in ESCC.Mechanistically,JOSD2 appears to activate the MAPK/ERK and PI3K/AKT signaling pathways.Mass spectrometry was used to identify crucial substrate proteins that interact with JOSD2,which identified the four primary proteins that bind to JOSD2,namely USP47,IGKV2D-29,HSP90AB1,and PRMT5.CONCLUSION JOSD2 plays a crucial role in enhancing the proliferation,migration,and drug resistance of ESCC,suggesting that JOSD2 is a potential therapeutic target in ESCC.
基金supported by the Natural Science Foundation of China (U22A20214)。
文摘Active Magnetic Bearing(AMB) is a kind of electromagnetic support that makes the rotor movement frictionless and can suppress rotor vibration by controlling the magnetic force. The most common approach to restrain the rotor vibration in AMBs is to adopt a notch filter or adaptive filter in the AMB controller. However, these methods cannot obtain the precise amplitude and phase of the compensation current. Thus, they are not so effective in terms of suppressing the vibrations of the fundamental and other harmonic orders over the whole speed range. To improve the vibration suppression performance of AMBs,an adaptive filter based on Least Mean Square(LMS) is applied to extract the vibration signals from the rotor displacement signal. An Iterative Search Algorithm(ISA) is proposed in this paper to obtain the corresponding relationship between the compensation current and vibration signals. The ISA is responsible for searching the compensating amplitude and shifting phase online for the LMS filter, enabling the AMB controller to generate the corresponding compensation force for vibration suppression. The results of ISA are recorded to suppress vibration using the Look-Up Table(LUT) in variable speed range. Comprehensive simulations and experimental validations are carried out in fixed and variable speed range, and the results demonstrate that by employing the ISA, vibrations of the fundamental and other harmonic orders are suppressed effectively.
基金supported by the Fuxing Nursing Research Foundation of Fudan University of China,No.FNF201611
文摘Symptoms that are multidimensional and concurrent should be assessed from different dimensions and managed together. Few studies have evaluated concurrent and multidimensional symptoms in patients with stroke. Most studies of stroke focused on dysfunctions and complications. We hypothesize that patients with stroke have a heavy symptom burden within 1 year. This study aimed to describe multidimensional and concurrent symptoms within 1 year after stroke. This study recruited 230 patients with stroke from the Rehabilitation Department of Xuhui District Center Hospital of Shanghai and the Shanghai Sunshine Rehabilitation Center in China from March to September 2017. The patients＇ multidimensional symptom experience and symptom burden were analyzed using a self-made structured questionnaire and the influential factors for symptom burden were identified. The mean number of symptoms in patients with stroke was 11.7 ± 3.5. More than two thirds of the participants suffered from at least 10 co-occurring symptoms. Unilateral limb weakness had the highest prevalence and frequency. Participation restriction had the highest symptom dimensions of severity and distress. Lack of self-care ability（severity）, memory deterioration（frequency）, imbalance of body（distress）, moodiness（distress）, being unable to move limbs at will（distress）, shoulder pain（distress）, and slower response（frequency） were independent factors of the total symptom burden score. These findings can provide essential information for efficient symptom management of patients with stroke.
文摘Background: Polysaccharides extracted from the medicinal herbs are known to possess antitumor effects. Although there is a significant number of evidences implicated on the beneficial effects of the ginseng leaves ginsenoside with diverse associated mechanisms, reports on the anticancer by the ginseng leaves crude polysaccharides (GLCP) are not sufficient. Experiments were carried out to unravel the anticancer effects of GLCP. Methods: Cells were treated with GLCP (0.5 - 2 mg/ml) for 48h. MTT method was used to detect the cell viability. Western blot and flow cytometry were used to detect apoptotic rate. Western blot and acridine orange staining were used to detect the cell autophagy. Results: Compared with the normal human liver cell (Chang liver), GLCP (1.5 - 2 mg/ml) significantly reduction cell viability, promote apoptosis-related proteins expression, promote cell apoptosis and autophagy in SMMC-7721 cells. But caveolin-1 gene silencing could inhibit the anticancer effect of GLCP. Conclusions: These data suggest that GLCP promote autophagy and apoptosis in human hepatoma cell SMMC-7721. We speculate that its mechanism may be associated with the caveolin-1 which is an essential structural molecule of caveolae. Although the effect of GLCP inhibited of liver cancer is not very strong, we are more interested in the GLCP which plays a tumor suppressor role in health care.
基金This work was supported by Chinese Academy of Social Sciences Peak Strategy Project“the Advantageous Discipline(Industrial Economics)”and Major Projects of National Social Science Foundation of China“Research on Promoting New Industrialization and Optimization and Upgradong of Economic System”[Grant number.21ZD021].
文摘This paper studies the environmental effects of technical change using a spatial model with panel data from 284 prefecture-cities over 2004-2015 in China.We find that the effects of technical change vary across different dimensions of technical change and different pollution indicators.Furthermore,we also provide robust evidence for the existence of the spatial effects of technical change on environmental pollution across cities.First,indigenous technical change displays three patterns of effects on the four pollutants:a positive effect on wastewater,a negative effect on PM_(2.5)concentrations,and an inverted U-shaped relationship with SO_(2)and soot emissions.The spatial effect of indigenous technical change promotes cleaner industrial productions(fewer emissions of SO_(2),soot and wastewater)but higher PM_(2.5)concentrations.Second,technology transfers from foreign direct investment are associated with less pollution except for wastewater,and their spatial effects are unanimously negative on all pollutants.Finally,absorptive capacity can also promote cleaner industrial production,but its spatial effects can do otherwise.Accordingly,the government should take the spatial spillover effects of technical change into account when implementing specific policies,pin down specific pollutants to make full use of the pollution-reducing effects of technical change,and improve the absorptive capacity of domestic firms.
基金Supported by the National Natural Science Foundation of China(31600543)Anhui Provincial Natural Science Foundation(1708085MC57)the State Forestry Administration,China("948"Project:#2014-4-52)
文摘Mutations induced from tissue culture are easily to be separated,which might be propagated in the same medium,especially for the color-leaf ornamental grass,Oxalis triaggularis purpurea.Mutations of the ornamental traits in the tissue culture bottle could be investigated easily.The 35 d regeneration system group showed the lowest adventitious bud number and adventitious root number among 4 inoculation dates by 50 Gy dose of ^(60)Coγ radiation.More studies were carried out based on the 35d differentiation state of O.triangularis purpurea regeneration system.The 35 d regeneration system was then irradiated by 10,25 and 50 Gy doses of ^(60)Coγ rays.Numbers of adventitious buds and roots induced from the regeneration system were cut down with the increment of radiation doses.Seedling length was not distinctly reduced at the absorbed doses of 10 and 25 Gy,but reduced distinctly under 50 Gy of ^(60)Coγ irradiation.The optimal irradiation dose for 35 d O.triangularis regeneration system survival and mutation induction was approximately 25 Gy.The M_2 phenotypic mutation rate was 2.9%,especially,and the leaf number mutation accounted for 76%of the total mutation.The phenotypic mutations,especially in the 10 Gy group,on 0.1 m M Vc containing MS medium were recovered,which indicated that ROS plays a key role in the phenotypic mutation induced by ^(60)Coγ -rays.
文摘The nuclear transcription factors κB (NF-κB) is widely existing in various kinds of cell types in the nervous system and plays an important role in neuron apoptosis and neurodegenerative diseases. Estrogen receptor alpha 36 (ER-α36), is a novel variant of ERα (as known ER-α66) which can transduce both estrogenand antiestrogen-dependent activation of MAPK signal pathway and stimulate cell growth. Here, we aimed to detect the effect of ER-α36 gene silencing on the expression of NF-κB in normal cultured PC12 cells and to provide an experimental foundation for understanding the function of ER-α36 innerve cells. PC12 cells with ER-α36 expression knocked down by the shRNA method. Then Western blot and immunocytochemical staining were performed to detect the expression and translocation of NF-κB after transfection. The results showed that NF-κB expression was significantly higher comparing with the control group after transfection (P 0.01). Also, NF-κB subunit entered nuclear after transfection;Immunofluorescence staining and immunocytochemical staining of PC12 cells demonstrated that ER-α36 was expressed mainly on the plasma membrane and on the cell nucleus membrane. These data indicate that ER-α36 gene silencing can increase the expression of NF-κB and promote its nuclear translocation in PC12 cells.
基金supported in part by the National Natural Science Foundation of China under Grant 52007161.
文摘Synchronous reluctance machine(SynRM)can be perceived as a special type of permanent magnet synchronous machine(PMSM),and shares similar control method,i.e.the sine-wave current drive with sinusoidal phase currents.In this paper,square-wave drive,which is employed for permanent magnet brushless(BLDC)motors,is employed for the SynRM,in order to economically reduce the cost of rotor position sensor.It is revealed that the torque density and efficiency are slightly sacrificed,whereas torque ripple is deteriorated,proving the SynRM with square-wave drive still promising for the cost-sensitive application if torque ripple is not considered as a critical issue.To further investigate the additional pulsating torque under square-wave drive,mathematical model based on a-b-c phase inductance and d-q axis inductance are established,together with the time-stepping FE calculated currents.It is concluded that the harmonics in the currents tend to cause non-sinusoidal variation of the magnetic reluctance,which can be represented as additional inductance harmonics.The harmonics of the current and inductance interact with each other,thus undesirable torque ripple components are produced.
基金National Natural Science Foundation of China(grant number 82088101 and 81930013 to Y.-H.C.,31871491 to J.Y.,82070338 and 82222008 to D.X.,82370396 to D.S.,82122007 to D.L.)National Key Research and Development Plan(grant number 2019YFA0801501 to Y.-H.C.)+4 种基金Program for the Research Unit of Origin and Regulation of Heart Rhythm,Chinese Academy of Medical Sciences(grant number 2019RU045 to Y.-H.C.)Top-Level Clinical Discipline Project of Shanghai Pudong(grant number PWYgf2021-01 to Y.-H.C.)Key Research Center Construction Project of Shanghai(grant number 2022ZZ01008 to Y.-H.C.)Shanghai Key Clinical Specialty Project(shslczdzk06202 to Y.-H.C.)National Key Clinical Specialty and Fundamental Research Funds for the Central Universities to Y.-H.C.
文摘Cardiac biological pacing(BP)is one of the future directions for bradyarrhythmias intervention.Currently,cardiac pacemaker cells(PCs)used for cardiac BP are mainly derived from pluripotent stem cells(PSCs).However,the production of high-quality cardiac PCs from PSCs remains a challenge.Here,we developed a cardiac PC differentiation strategy by adopting dual PC markers and simulating the developmental route of PCs.First,two PC markers,Shox2 and Hcn4,were selected to establish Shox2:EGFP;Hcn4:mCherry mouse PSC reporter line.Then,by stepwise guiding naïve PSCs to cardiac PCs following naïve to formative pluripotency transition and manipulating signaling pathways during cardiac PCs differentiation,we designed the FSK method that increased the yield of SHOX2^(+);HCN4^(+)cells with typical PC characteristics,which was 12 and 42 folds higher than that of the embryoid body(EB)and the monolayer M10 methods respectively.In addition,the in vitro cardiac PCs differentiation trajectory was mapped by single-cell RNA sequencing(scRNA-seq),which resembled in vivo PCs development,and ZFP503 was verified as a key regulator of cardiac PCs differentiation.These PSC-derived cardiac PCs have the potential to drive advances in cardiac BP technology,help with the understanding of PCs(patho)physiology,and benefit drug discovery for PC-related diseases as well.
基金supported by the National Natural Science Foundation of China (82088101,81930013,82000377,31871491)the National Key Research and Development Plan (2019YFA0801501)+5 种基金Key Research Center Construction Project of Shanghai (2022ZZ01008)Shanghai Key clinical specialty Project (shslczdzk06202)Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai (PWZxq2017-05)Top-level Clinical Discipline Project of Shanghai Pudong District (PWYgf2021-01)Program for the Research Unit of Origin and Regulation of Heart Rhythm,Chinese Academy of Medical Sciences (2019RU045)Innovative research team of high-level local universities in Shanghai and a key laboratory program of the Education Commission of Shanghai Municipality (ZDSYS14005)。
文摘Histone modifications play crucial roles in the pathogenesis of myocardial ischaemia/reperfusion(I/R)injury.However,a genome-wide map of histone modifications and the underlying epigenetic signatures in myocardial I/R injury have not been established.Here,we integrated transcriptome and epigenome of histone modifications to characterize epigenetic signatures after I/R injury.Disease-specific histone mark alterations were mainly found in H3K27me3-,H3K27ac-,and H3K4me1-marked regions 24 and 48 h after I/R.Genes differentially modified by H3K27ac,H3K4me1 and H3K27me3 were involved in immune response,heart conduction or contraction,cytoskeleton,and angiogenesis.H3K27me3 and its methyltransferase polycomb repressor complex 2(PRC2)were upregulated in myocardial tissues after I/R.Upon selective inhibition of EZH2(the catalytic core of PRC2),the mice manifest improved cardiac function,enhanced angiogenesis,and reduced fibrosis.Further investigations confirmed that EZH2 inhibition regulated H3K27me3 modification of multiple pro-angiogenic genes and ultimately enhanced angiogenic properties in vivo and in vitro.This study delineates a landscape of histone modifications in myocardial I/R injury,and identifies H3K27me3 as a key epigenetic modifier in I/R process.The inhibition of H3K27me3 and its methyltransferase might be a potential strategy for myocardial I/R injury intervention.
基金supported by grants from the 135 Important Medical Project of Health Department of Jiangsu Province (No. K0621)
文摘B cell activating factor belonging to TNF superfamily （BAFF） is a critical regulator of B cell maturation and survival In this present study, the expression characteristic of BAFF in kidney transplantation recipients was investigated, its potential significance was analyzed and peripheral blood of follow-up kidney transplant recipients was studied. Flow cytometric assay results showed that, cell-surface BAFF was significantly highly expressed on peripheral CD3＋ T lymphocytes in 〉 5 yrs group of kidney transplant recipients, compared with other groups （p 〈 0.05）. BAFF expression could be found on CD4＋ T cells and CD8＋ T cells. The BAFF mRNA levels in peripheral mononuclear cells were consistent with the protein levels. However, serum soluble BAFF levels were inter- individually different in each group. Stratified by renal function, it was found that cell-surface BAFF levels were significantly higher in those with abnormal renal function, compared with recipients with normal renal function （p 〈 0.05）. ELISA assay results showed that expression levels of cell surface BAFF were significantly correlated with anti-HLA I ＆ II antibodies. These results indicate that BAFF may be involved in the development of graft-loss and influences the long-time outcome of kidney allografl, likely mediated by interfering with immune response. Cellular ＆ Molecular Immunology. 2008;5（6）：465-470.
基金financially supported by the National Natural Science Foundation of China (31702362)the Applied Basic Research Programs of Science and Technology Commission Foundation of Sichuan Province,China (grant number 2015JY0067).
文摘Mitogen-activated protein kinases(MAPK) and nuclear factor kappa B(NF-κB) pathways are considered to be two crucial intracellular signaling cascades in pro-inflammatory responses.In this study,we reported the coding sequences(CDS) of MAPKp38 and NF-κBp65 from yellow catfish.We also investigated the gene structure,expression patterns and functional role in yellow catfish.The CDS of MAPKp38 is1,086 bp encoding 361 amino acids(AA).The MAPKp38 protein has a long highly conserved serine/threonine protein kinases catalytic domain.The NF-κBp65 CDS is 1,794 bp,and the gene encodes 597 AA,with a Rel homology domain(RHD) which consists of a RHD-DNA-binding domain and an Ig-like,plexins,transcription factors(IPT) domain.Moreover,MAPKp38 and NF-κBp65 protein of bony fish and other vertebrates have a single clade.Quantitative real-time PCR analysis revealed the presence of the MAPKp38 and NF-κBp65 transcript in 12 tissues of healthy yellow catfish.The highest expression levels of MAPKp38 and NF-κBp65 were detected in the heart and liver,respectively.Upon stimulation with an intraperitoneal injection of lipopolysaccharide(LPS),the expression levels of MAPKp38 and NF-κBp65 were up-regulated in the intestine.These results indicated that MAPKp38 and NF-κBp65 play important roles in mediating the response protection against LPS in yellow catfish.
文摘At present, the development of renewable energy relies mainly on government support. The government invests in a considerable number of projects to improve public welfare and to assist in poverty relief If China is to replace fossil fuels on a large scale with renewable energy sources, the production costs and prices of renewable energy must be brought down. All countries are facing the challenge of moving to a more secure and low-carbon energy system without weakening economic and social development. In this regard, China is facing an even greater challenge in terms of economic cost, as cheap coal remains the main energy form. Technical innovation and industrialization in the area of renewable energy is an important means of lowering cost. China is in for a period of high-speed development of its economy and the rising demand for energy is irreversible. If the technical progress and development speed of renewable energy lags behind the growth in demand, it will be difficult to realize the improvement of its energy structure.
基金supported by Program for New Century Excellent Talents in University(NCET-11-0252)the MOE Project of Key Research Institute of Humanities and Social Sciences at Universities(12JJD630004)+1 种基金Program for Changjiang Scholars and Innovative Research Team in University(IRT0926)Research Grants Council of Hong Kong under General Research Fund No.410509,and NSFC Key Program Grant No.70932005
文摘In this paper, we develop models to determine operational and financial decisions of a supply chain under the condition that the retailer faces a financial constraint and the manufacturer can offer trade credit to assist the retailer. We first study the case where the retailer is risk-neutral, and derive the optimal ordering and financial decisions. Then, the case where the retailer is risk-averse （downside risk） is studied and-the effects of the risk on the retailer and manufacturer＇s operational and financial decisions are discussed. Finally, numerical examples are provided to conduct managerial analysis.
基金financial support from the Project supported by the National Natural Science Foundation of China(Grant No.51202257)Shenyang Double-Hundreds Project(Z17-7-027,Z18-0-025)
文摘In this study, diamond films were synthesized on silicon substrates by microwave plasma enhanced chemical vapor deposition（CVD） over a wide range of experimental parameters. The effects of the microwave power,CH;/H;ratio and gas pressure on the morphology, growth rate, composition, and quality of diamond films were investigated by means of scanning electron microscope（SEM）, X-ray diffraction（XRD）, Raman spectroscopy and X-ray photoelectron spectroscopy（XPS）. A rise of microwave power can lead to an increasing pyrolysis of hydrogen and methane, so that the microcrystalline diamond film could be synthesized at low CH;/H;levels. Gas pressure has similar effect in changing the morphology of diamond films, and high gas pressure also results in dramatically increased grain size. However,diamond film is deteriorated at high CH;/H;ratio due to the abundant graphite content including in the films. Under an extreme condition of high microwave power of 10 kW and high CH;concentration, a hybrid film composed of diamond/graphite was successfully formed in the absence of N;or Ar,which is different from other reports. This composite structure has an excellent measured sheet resistance of 10-100 Ω/Sqr. which allows it to be utilized as field electron emitter. The diamond/graphite hybrid nanostructure displays excellent electron field emission（EFE） properties with a low turn-on field of 2.17 V/μm and β= 3160, therefore it could be a promising alternative in field emission applications.
基金financially supported by the Joint Research Fund Liaoning-Shenyang National Laboratory for Materials Science(No.20180510009)the Young Talent Program of Shenyang National Laboratory for Materials Science(No.L2019F39)the National Natural Science Foundation of China(No.51202257)。
文摘Tremendous demands for highly sensitive and stable seawater salinometers have motivated intensive research on advanced electrode materials.Boron-doped diamond(BDD)is attractive in terms of its high mechanical stability and chemical inertness,but is usually hindered by its low double-layer capacitance(C_(dl))for seawater salinity detection.Here,inspired by the principle of oxygen-terminated BDD electrode endowing higher C_(dl)than hydrogen-terminated surface,we introduce the oxygen terminated surface by oxygen plasma or reactive ion etch(RIE),and the fabricated oxygen terminated BDD electrodes demonstrate high sensitivity and long-term stability in seawater salinity detection comparing with the hydrogen terminated BDD electrodes.Significantly,the as-fabricated O-BDD-RIE electrodes not only show remarkable enhanced response even better than the commercial platinum black electrodes but also display long-time stability which is weekly verified by continuous monitor for 90 days.The outstanding performance of the oxygen terminated BDD electrodes can be ascribed to the enhancement of C-O surface functional group on C_(dl).In addition,a comprehensive analysis of effective electroactive surface area(EASA)and C_(dl)proves that the surface oxygen is the major factor for the improved C_(dl).In summary,the excellent oxygen terminated BDD electrodes promise potential application in seawater salinity detection.
