AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor.METHODS:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytom...AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor.METHODS:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytometric,osteogenic and adipogenic induction.Xenogenic decellularized corneal matrix(XDCM)was generated from dog corneas.MSCs were seeded and cultured on XDCM to construct the tissueengineered cornea.Post-transplantation biocompatibility of engineered corneal graft were tested by animal experiment.Rabbits were divided into two groups then underwent lamellar keratoplasty(LK)with different corneal grafts:1)XDCM group(n=5):XDCM;2)XDCM-MSCs groups(n=4):tissue-engineered cornea made up with XDCM and MSCs.The ocular surface recovery procedure was observed while corneal transparency,neovascularization and epithelium defection were measured and compared.In vivo on focal exam was performed 3 mo postoperatively.RESULTS:Rabbit MSCs were isolated and identified.Flow cytometry demonstrated isolated cells were CD90 positive and CD34,CD45 negative.Osteogenic and adipogenic induction verified their multipotent abilities.MSC-XDCM grafts were constructed and observed.In vivo transplantation showed the neovascularization in XDCMMSC group was much less than that in XDCM group postoperatively.Post-transplant 3-month confocal test showed less nerve regeneration and bigger cell-absent area in XDCM-MSC group.CONCLUSION:This study present a novel corneal tissue-engineered graft that could reduce post-operatively neovascularization and remain transparency,meanwhile shows that co-transplantation of MSCs may help increase corneal transplantation successful rate and enlarge the source range of corneal substitute to overcome cornea donor shortage.展开更多
BACKGROUND Choristoma is a rare,benign,congenital proliferative tumor,with osseous choristoma being the rarest.Although the tumor is benign,effective identification is needed for its diagnosis and treatment.Here,we re...BACKGROUND Choristoma is a rare,benign,congenital proliferative tumor,with osseous choristoma being the rarest.Although the tumor is benign,effective identification is needed for its diagnosis and treatment.Here,we report the diagnosis and successful surgical treatment of two patients with osseous choristoma.CASE SUMMARY Two patients,a young female and young male patient,were found to have a mass on the ocular surface.The tumor presented on the superior temporal bulbar conjunctiva in the first patient and on the upper eyelid in the second patient.Ultrasound biomicroscopy detected a strong echo with clear boundaries covering the lower echo,and computed tomography examination revealed calcification.Both patients underwent surgery,and histopathological evaluation of the mass showed osseous choristoma.They were treated by excision and subsequently cured.CONCLUSION Osseous choristomas are usually asymptomatic.Our patients were cured immediately after surgery,suggesting that surgical treatment is an effective strategy.展开更多
基金Supported by National Natural Science Foundation of China(No.81700799)Clinical Medicine Plus X-Young Scholar Project,Peking University。
文摘AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor.METHODS:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytometric,osteogenic and adipogenic induction.Xenogenic decellularized corneal matrix(XDCM)was generated from dog corneas.MSCs were seeded and cultured on XDCM to construct the tissueengineered cornea.Post-transplantation biocompatibility of engineered corneal graft were tested by animal experiment.Rabbits were divided into two groups then underwent lamellar keratoplasty(LK)with different corneal grafts:1)XDCM group(n=5):XDCM;2)XDCM-MSCs groups(n=4):tissue-engineered cornea made up with XDCM and MSCs.The ocular surface recovery procedure was observed while corneal transparency,neovascularization and epithelium defection were measured and compared.In vivo on focal exam was performed 3 mo postoperatively.RESULTS:Rabbit MSCs were isolated and identified.Flow cytometry demonstrated isolated cells were CD90 positive and CD34,CD45 negative.Osteogenic and adipogenic induction verified their multipotent abilities.MSC-XDCM grafts were constructed and observed.In vivo transplantation showed the neovascularization in XDCMMSC group was much less than that in XDCM group postoperatively.Post-transplant 3-month confocal test showed less nerve regeneration and bigger cell-absent area in XDCM-MSC group.CONCLUSION:This study present a novel corneal tissue-engineered graft that could reduce post-operatively neovascularization and remain transparency,meanwhile shows that co-transplantation of MSCs may help increase corneal transplantation successful rate and enlarge the source range of corneal substitute to overcome cornea donor shortage.
文摘BACKGROUND Choristoma is a rare,benign,congenital proliferative tumor,with osseous choristoma being the rarest.Although the tumor is benign,effective identification is needed for its diagnosis and treatment.Here,we report the diagnosis and successful surgical treatment of two patients with osseous choristoma.CASE SUMMARY Two patients,a young female and young male patient,were found to have a mass on the ocular surface.The tumor presented on the superior temporal bulbar conjunctiva in the first patient and on the upper eyelid in the second patient.Ultrasound biomicroscopy detected a strong echo with clear boundaries covering the lower echo,and computed tomography examination revealed calcification.Both patients underwent surgery,and histopathological evaluation of the mass showed osseous choristoma.They were treated by excision and subsequently cured.CONCLUSION Osseous choristomas are usually asymptomatic.Our patients were cured immediately after surgery,suggesting that surgical treatment is an effective strategy.
