BACKGROUND Capecitabine(CAP)is a classic antimetabolic drug and has shown potential antirejection effects after liver transplantation(LT)in clinical studies.Our previous study showed that metronomic CAP can cause the ...BACKGROUND Capecitabine(CAP)is a classic antimetabolic drug and has shown potential antirejection effects after liver transplantation(LT)in clinical studies.Our previous study showed that metronomic CAP can cause the programmed death of T cells by inducing oxidative stress in healthy mice.Ferroptosis,a newly defined non-apoptotic cell death that occurs in response to iron overload and lethal levels of lipid peroxidation,is an important mechanism by which CAP induces cell death.Therefore,ferroptosis may also play an important role in CAP-induced T cell death and play an immunosuppressive role in acute rejection after transplantation.AIM To investigate the functions and underlying mechanisms of antirejection effects of metronomic CAP.METHODS A rat LT model of acute rejection was established,and the effect of metronomic CAP on splenic hematopoietic function and acute graft rejection was evaluated 7 d after LT.In vitro,primary CD3+T cells were sorted from rat spleens and human peripheral blood,and co-cultured with or without 5-fluorouracil(5-FU)(active agent of CAP).The levels of ferroptosis-related proteins,ferrous ion concentration,and oxidative stress-related indicators were observed.The changes in mitochondrial structure were observed using electron microscopy.RESULTS With no significant myelotoxicity,metronomic CAP alleviated graft injury(Banff score 9 vs 7.333,P<0.001),prolonged the survival time of the recipient rats(11.5 d vs 16 d,P<0.01),and reduced the infiltration rate of CD3+T cells in peripheral blood(6.859 vs 3.735,P<0.001),liver graft(7.459 vs 3.432,P<0.001),and spleen(26.92 vs 12.9,P<0.001),thereby inhibiting acute rejection after LT.In vitro,5-FU,an end product of CAP metabolism,induced the degradation of the ferritin heavy chain by upregulating nuclear receptor coactivator 4,which caused the accumulation of ferrous ions.It also inhibited nuclear erythroid 2 p45-related factor 2,heme oxygenase-1,and glutathione peroxidase 4,eventually leading to oxidative damage and ferroptosis of T cells.CONCLUSION Metronomic CAP can suppress acute allograft rejection in rats by triggering CD3+T cell ferroptosis,which makes it an effective immunosuppressive agent after LT.展开更多
The surfaces of X80 pipeline steel welded joints were processed with a CO2 laser, and the effects of laser heat treatment(LHT) on H2 S stress corrosion in the National Association of Corrosion Engineers(NACE) solution...The surfaces of X80 pipeline steel welded joints were processed with a CO2 laser, and the effects of laser heat treatment(LHT) on H2 S stress corrosion in the National Association of Corrosion Engineers(NACE) solution were analyzed by a slow strain rate test. The fracture morphologies and chemical components of corrosive products before and after LHT were analyzed by scanning electron microscopy and energy-dispersive spectroscopy, respectively, and the mechanism of LHT on stress corrosion cracking was discussed. Results showed that the fracture for welded joints was brittle in its original state, while it was transformed to a ductile fracture after LHT. The tendencies of hydrogen-induced corrosion were reduced, and the stress corrosion sensitivity index decreased from 35.2% to 25.3%, indicating that the stress corrosion resistance of X80 pipeline steel welded joints has been improved by LHT.展开更多
DEAR EDITOR,The green peafowl (Pavo muticus) is one of the most threatened pheasants in the world. In China, it is widely appreciated for its beauty as well as historical and cultural value, but current populations nu...DEAR EDITOR,The green peafowl (Pavo muticus) is one of the most threatened pheasants in the world. In China, it is widely appreciated for its beauty as well as historical and cultural value, but current populations number less than 500 individuals. Recently, Tang and colleagues reported in Science that the green peafowl is likely to become extinct due to the construction of the Jiasajiang Level 1 Hydropower Station within the Red River Upstream District (RRUD) and thus called for a stop to this project (Tang et al., 2019). According to our recent surveys, however, this species is still extant in 22 counties of Yunnan Province, China, among which, only two within the RRUD have been predicted to be affected by floods from the hydropower station. Therefore, the conclusion that the species will likely go extinct in China upon completion of the dam is unwarranted. In fact, construction of the Jiasajiang Level 1 Hydropower Station was stopped in August 2017. The main challenge for green peafowl conservation is that over 65% of the population occurs outside of protected reserves in China. Fortunately, the Chinese government has adopted an Ecological Redline (ERL) strategy to achieve ecological civilization plans, thus bringing new hope to the conservation of green peafowls both inside and outside of protected reserves. As a top conservation priority for China, the government is fully committed to conserving this peafowl.展开更多
AlCrN coating was prepared on the surface of TiC-based cermet cutting tool with cathodic arc ion plating,and the surface-interface morphologies,compositions of chemical elements,and phases were analyzed by scanning el...AlCrN coating was prepared on the surface of TiC-based cermet cutting tool with cathodic arc ion plating,and the surface-interface morphologies,compositions of chemical elements,and phases were analyzed by scanning electron microscope(SEM),energy dispersive spectrometer(EDS),and X-ray diffraction(XRD),respectively.Bonding strength of the coating-substrate interface was measured with scratching test,and the wear mechanism and friction coefficient of TiC-based cermet cutting tool and AlCrN coating were investigated with friction and wear testing machine.The results show that the interface binding of AlCrN coating is metallurgical mode,and its binding strength is 83 N.The average friction coefficient of TiC-based cermet cutting tool under the load of 6 N is 0.8409,while that under the load of 8 N decreases by about 11%,and the wear mechanism of TiC cermet cutting tool is fatigue wear,abrasive wear,and oxidation wear.The average friction coefficient of AlCrN coating is 0.6363 under the load of 6 N,and the change is relatively stable,decreasing by about 25%than that of TiC-based cermet cutting tool;when the load increases to 8 N,the average friction coefficient of AlCrN coating decreases by 9%.The wear mechanism of AlCrN coating is abrasive wear and oxidation wear,showing lower friction coefficient and good wear resistance.展开更多
基金Supported by National Key Research and Development Program of China,No.2020YFA0710802The Youth Science Fund of the Nature Science Foundation of Tianjin,No.20JCQNJC01370+1 种基金The Key Projects of Tianjin Science and Technology Project,No.21JCZDJC00160The Science Foundation of Tianjin Health Commission,No.ZC20065 and No.ZC20089.
文摘BACKGROUND Capecitabine(CAP)is a classic antimetabolic drug and has shown potential antirejection effects after liver transplantation(LT)in clinical studies.Our previous study showed that metronomic CAP can cause the programmed death of T cells by inducing oxidative stress in healthy mice.Ferroptosis,a newly defined non-apoptotic cell death that occurs in response to iron overload and lethal levels of lipid peroxidation,is an important mechanism by which CAP induces cell death.Therefore,ferroptosis may also play an important role in CAP-induced T cell death and play an immunosuppressive role in acute rejection after transplantation.AIM To investigate the functions and underlying mechanisms of antirejection effects of metronomic CAP.METHODS A rat LT model of acute rejection was established,and the effect of metronomic CAP on splenic hematopoietic function and acute graft rejection was evaluated 7 d after LT.In vitro,primary CD3+T cells were sorted from rat spleens and human peripheral blood,and co-cultured with or without 5-fluorouracil(5-FU)(active agent of CAP).The levels of ferroptosis-related proteins,ferrous ion concentration,and oxidative stress-related indicators were observed.The changes in mitochondrial structure were observed using electron microscopy.RESULTS With no significant myelotoxicity,metronomic CAP alleviated graft injury(Banff score 9 vs 7.333,P<0.001),prolonged the survival time of the recipient rats(11.5 d vs 16 d,P<0.01),and reduced the infiltration rate of CD3+T cells in peripheral blood(6.859 vs 3.735,P<0.001),liver graft(7.459 vs 3.432,P<0.001),and spleen(26.92 vs 12.9,P<0.001),thereby inhibiting acute rejection after LT.In vitro,5-FU,an end product of CAP metabolism,induced the degradation of the ferritin heavy chain by upregulating nuclear receptor coactivator 4,which caused the accumulation of ferrous ions.It also inhibited nuclear erythroid 2 p45-related factor 2,heme oxygenase-1,and glutathione peroxidase 4,eventually leading to oxidative damage and ferroptosis of T cells.CONCLUSION Metronomic CAP can suppress acute allograft rejection in rats by triggering CD3+T cell ferroptosis,which makes it an effective immunosuppressive agent after LT.
基金This study was supported by the National Natural Science Foundation of China(31201725)the Applicable Basic research Project of Yunnan Province(2012FB186)the Western Light Talents Programme of Chinese Academy of Sciences.
基金financially supported by the Chief Expert (Engineer) Project of Jiangsu Provincial Association for Science (No. 2012-09)
文摘The surfaces of X80 pipeline steel welded joints were processed with a CO2 laser, and the effects of laser heat treatment(LHT) on H2 S stress corrosion in the National Association of Corrosion Engineers(NACE) solution were analyzed by a slow strain rate test. The fracture morphologies and chemical components of corrosive products before and after LHT were analyzed by scanning electron microscopy and energy-dispersive spectroscopy, respectively, and the mechanism of LHT on stress corrosion cracking was discussed. Results showed that the fracture for welded joints was brittle in its original state, while it was transformed to a ductile fracture after LHT. The tendencies of hydrogen-induced corrosion were reduced, and the stress corrosion sensitivity index decreased from 35.2% to 25.3%, indicating that the stress corrosion resistance of X80 pipeline steel welded joints has been improved by LHT.
文摘DEAR EDITOR,The green peafowl (Pavo muticus) is one of the most threatened pheasants in the world. In China, it is widely appreciated for its beauty as well as historical and cultural value, but current populations number less than 500 individuals. Recently, Tang and colleagues reported in Science that the green peafowl is likely to become extinct due to the construction of the Jiasajiang Level 1 Hydropower Station within the Red River Upstream District (RRUD) and thus called for a stop to this project (Tang et al., 2019). According to our recent surveys, however, this species is still extant in 22 counties of Yunnan Province, China, among which, only two within the RRUD have been predicted to be affected by floods from the hydropower station. Therefore, the conclusion that the species will likely go extinct in China upon completion of the dam is unwarranted. In fact, construction of the Jiasajiang Level 1 Hydropower Station was stopped in August 2017. The main challenge for green peafowl conservation is that over 65% of the population occurs outside of protected reserves in China. Fortunately, the Chinese government has adopted an Ecological Redline (ERL) strategy to achieve ecological civilization plans, thus bringing new hope to the conservation of green peafowls both inside and outside of protected reserves. As a top conservation priority for China, the government is fully committed to conserving this peafowl.
基金financially supported by the Jiangsu Province Science and Technology Support Program(Industry)(No.BE2014818)。
文摘AlCrN coating was prepared on the surface of TiC-based cermet cutting tool with cathodic arc ion plating,and the surface-interface morphologies,compositions of chemical elements,and phases were analyzed by scanning electron microscope(SEM),energy dispersive spectrometer(EDS),and X-ray diffraction(XRD),respectively.Bonding strength of the coating-substrate interface was measured with scratching test,and the wear mechanism and friction coefficient of TiC-based cermet cutting tool and AlCrN coating were investigated with friction and wear testing machine.The results show that the interface binding of AlCrN coating is metallurgical mode,and its binding strength is 83 N.The average friction coefficient of TiC-based cermet cutting tool under the load of 6 N is 0.8409,while that under the load of 8 N decreases by about 11%,and the wear mechanism of TiC cermet cutting tool is fatigue wear,abrasive wear,and oxidation wear.The average friction coefficient of AlCrN coating is 0.6363 under the load of 6 N,and the change is relatively stable,decreasing by about 25%than that of TiC-based cermet cutting tool;when the load increases to 8 N,the average friction coefficient of AlCrN coating decreases by 9%.The wear mechanism of AlCrN coating is abrasive wear and oxidation wear,showing lower friction coefficient and good wear resistance.
