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phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L.Burtt 被引量:2
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作者 Suprava Sahoo Goutam Ghosh +1 位作者 debajyoti das Sanghamitra Nayak 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第11期871-876,共6页
Objective:To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.Methods:The study was done by using various in vitro methods such as 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2-azino-bis(3-ethyl... Objective:To investigate antioxidant potential of methanol extract of Alpinia nigra leaves.Methods:The study was done by using various in vitro methods such as 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)(ABTS),nitric oxide and hydrogen peroxide radical scavenging assays.Phytochemical constituents,total phenolic content and total flavonoid content of the extract at different concentrations(10-500μg/mL)were determined.Results:Alpinia nigra leaves showed high free radical scavenging activity as evidenced by the low IG_(50)values in DPPH(64.51μg/mL),in ABTS(28.32μg/mL),in nitric oxide(80.02μg/mL)and in H_2O_2(77.45μg/mL)scavenging assays.Furthermore the TPC and TFC of the extract were found to be 69.25 mg gallic acid equivalent per gram of extract and 78.84 mg quercetin equivalent per gram of extract respectively.Conclusions:The results of present comprehensive analysis demonstrated that Alpinia nigra leaves possess high phenolic,flavonoid contents and potential antioxidant activity,and could be used as a viable source of natural antioxidants and might be exploited for functional foods and neutraceutical applications. 展开更多
关键词 ALPINIA nigra PHYTOCHEMICAL screening TPC TFC Antioxidant activity
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