[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assiste...[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.展开更多
[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavono...[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavonoids in ginkgo leaves were studied,and the optimal extraction conditions for flavonoids were determined by orthogonal test; and with quercetin as reference substance,total flavonoid content in ginkgo leaves was determined by UV spectrophotometry.[Results]The optimal extraction process was 4 h of Soxhlet extraction with methanol; and the total flavonoid contents had a good linear relation in the range of 0. 006 5-0. 039 mg/ml( R^2= 0. 999 9),the average content was stabilized at 1. 135%,and the average recovery of the method was 102. 0%. [Conclusions]This study selected the optimal extraction process for total flavonoids in ginkgo leaves. The test method is simple with high accuracy and precision,and is suitable for the extraction and determination of total flavonoids in ginkgo leaves.展开更多
Ampelopsis grossedentata(Hand-Mazz) W. T. wang is a typical edible plant with important physiological health functions. Dihydromyricetin is the main active ingredient of A. grossedentata, which has good pharmacologica...Ampelopsis grossedentata(Hand-Mazz) W. T. wang is a typical edible plant with important physiological health functions. Dihydromyricetin is the main active ingredient of A. grossedentata, which has good pharmacological effects such as antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and liver protecting effects. This paper summarized the research progress on the extraction materials, extraction methods and separation and purification processes of dihydromyricetin in A. grossedentata to understand the best experimental method of dihydromyricetin, aiming to provide an important theoretical basis for the comprehensive development and utilization of dihydromyricetin.展开更多
[Objectives] This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the conten...[Objectives] This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the content of quercetin in ferment of G.biloba leaves was determined by reversed-phase high-performance liquid chromatography.First,the flavonoid glycosides were extracted with methanol.Then,the flavonoid glycosides were hydrolyzed with hydrochloric acid to prepare the test solution.The chromatographic conditions were as follows:Platisil ODS C_(18) column(150 mm × 4.6 mm,5 μm);V_(methonal)∶V_(water)(0.4% phosphoric acid solution) =55∶45;flow rate of 1 m L/min;Shimadzu UV detector;detection wavelength of 360 nm.[Results] Quercetin was used as a reference substance.In the range of 0.002 6-0.036 0 g/L,there was a good linear relationship,with correlation coefficient of 0.999 8 and RSD of 1.26%.[Conclusions] This method is simple,easy to operate,accurate,and reproducible.It is suitable for the determination of quercetin content in G.biloba leaves.展开更多
[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as indep...[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good.展开更多
[Objectives] This study was conducted to determine kaempferol content in ginkgo( Ginkgo biloba L.) leaves subjected to microbial fermentation.[Methods]Bacillus licheniformis was selected for solid-state fermentation o...[Objectives] This study was conducted to determine kaempferol content in ginkgo( Ginkgo biloba L.) leaves subjected to microbial fermentation.[Methods]Bacillus licheniformis was selected for solid-state fermentation of ginkgo leaves,and the content of kaempferol in ginkgo leaves was determined by RPHPLC method. At first,methanol was used to extract flavonoid glycosides,which were then hydrolyzed by hydrochloric acid solution. HPLC was performed with Platisil ODS column C18( 150 mm ×4. 6 mm,5 μm) using mobile phase Vmethanol∶ Vwater( 0. 4% phosphoric acid solution) = 55∶45 at a flow rate of 1 ml/min,and the eluate was detected with a shimadzu HPLC ultraviolet detector at 360 nm. [Results]With kaempferol as the reference substance,the correlation coefficient was0. 999 2 in the range of 0. 001 06-0. 016 96 g/L. The content in the fermented product was less than that in the non-fermented product by 28%. [Conclusions]The method is simple,accurate,and is suitable for determination of kaempferol. This study will provide an experimental basis for the development and utilization of ginkgo.展开更多
[Objectives]This study was conducted to determine the content of total flavonoids in ginkgo ( Ginkgo biloba L.) leaves.[Methods]The content of total flavonoids from ginkgo leaves was determined by reversed phase-high ...[Objectives]This study was conducted to determine the content of total flavonoids in ginkgo ( Ginkgo biloba L.) leaves.[Methods]The content of total flavonoids from ginkgo leaves was determined by reversed phase-high performance liquid chromatography (RP-HPLC).The flavonoid glycosides were first extracted with methanol,and hydrolyzed with hydrochloric acid solution to prepare a test solution.Platisil ODS C18 column (150 mm×4.6 mm,5 μm) and the mobile phase V methanol ∶ V water (0.4% phosphoric acid solution)=85∶ 15 were selected for HPLC separation.The HPLC separation was performed with the column at a column temperature of 25℃ using the mobile phase at a flow rate of 1 ml/min.The sample size was 10 μl,and detection was performed with an Agilent HPLC ultraviolet detector at 360 nm.[Results]The reference substance,quercetin,had good linearity in the range of 0.002 6-0.052 0 g/L,with a correlation coefficient of 0.999 7;and the RSD was 1.26%.[Conclusions]The determination method has rapid and simple operation with accurate results and is good in repeatability.This method is suitable for the determination of content of total flavonoids in ginkgo leaves.展开更多
[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and pho...[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy.展开更多
基金Supported by the Program of Guilin Municipal Science and Technology Bureau(20130403-4)Science and Technology Program of Traditional Chinese Medicine from Guangxi Administration of Traditional Chinese Medicine(GZLC14-31)Undergraduate Innovation and Entrepreneurship Training Plan Project of Autonomous District Level in 2017(201710601082)
文摘[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.
基金Supported by Guilin Science and Technology Bureau Project(20100305)Guangxi"2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(G2013[20])Special Fund for Traditional Medical Science and Technology of Department of Public Health of Guangxi Zhuang Autonomous Region(GZMZ1212)
文摘[Objectives]This study was conducted to study the extraction process and the content determination of flavonoids in ginkgo( Ginkgo biloba L.) leaves.[Methods]Ethanol extraction and methanol extraction of total flavonoids in ginkgo leaves were studied,and the optimal extraction conditions for flavonoids were determined by orthogonal test; and with quercetin as reference substance,total flavonoid content in ginkgo leaves was determined by UV spectrophotometry.[Results]The optimal extraction process was 4 h of Soxhlet extraction with methanol; and the total flavonoid contents had a good linear relation in the range of 0. 006 5-0. 039 mg/ml( R^2= 0. 999 9),the average content was stabilized at 1. 135%,and the average recovery of the method was 102. 0%. [Conclusions]This study selected the optimal extraction process for total flavonoids in ginkgo leaves. The test method is simple with high accuracy and precision,and is suitable for the extraction and determination of total flavonoids in ginkgo leaves.
基金Supported by Project of Guilin Science and Technology Bureau(20140105-12)
文摘Ampelopsis grossedentata(Hand-Mazz) W. T. wang is a typical edible plant with important physiological health functions. Dihydromyricetin is the main active ingredient of A. grossedentata, which has good pharmacological effects such as antibacterial, anti-inflammatory, anti-oxidation, anti-tumor and liver protecting effects. This paper summarized the research progress on the extraction materials, extraction methods and separation and purification processes of dihydromyricetin in A. grossedentata to understand the best experimental method of dihydromyricetin, aiming to provide an important theoretical basis for the comprehensive development and utilization of dihydromyricetin.
基金Supported by Project of Guilin Science and Technology Bureau(20100305)Guangxi Collaborative Innovation Center:Zhuang Yao Medicine Collaborative Innovation Center(Gui 2013[20])Guangxi Traditional Chinese Medicine Science and Technology Project(GZMZ1202)
文摘[Objectives] This study aimed to determine the content of quercetin in ferment of Ginkgo biloba L.leaves.[Methods]Bacillus licheniformis was selected for solid-state fermentation of G.biloba leaf powder,and the content of quercetin in ferment of G.biloba leaves was determined by reversed-phase high-performance liquid chromatography.First,the flavonoid glycosides were extracted with methanol.Then,the flavonoid glycosides were hydrolyzed with hydrochloric acid to prepare the test solution.The chromatographic conditions were as follows:Platisil ODS C_(18) column(150 mm × 4.6 mm,5 μm);V_(methonal)∶V_(water)(0.4% phosphoric acid solution) =55∶45;flow rate of 1 m L/min;Shimadzu UV detector;detection wavelength of 360 nm.[Results] Quercetin was used as a reference substance.In the range of 0.002 6-0.036 0 g/L,there was a good linear relationship,with correlation coefficient of 0.999 8 and RSD of 1.26%.[Conclusions] This method is simple,easy to operate,accurate,and reproducible.It is suitable for the determination of quercetin content in G.biloba leaves.
基金Supported by Chinese Medicine Science and Technology Project of Guangxi Administration of Traditional Chinese Medicine(GZLC14-31)Science and Technology Research and Development Program of Guilin Bureau of Technology(20130403-4)+1 种基金Guangxi"2011 Collaborative Innovation Center"-Zhuang and Yao Medicine Collaborative Innovation Center(Gui201320)the Autonomous Region-Level College Students’ Innovation and Entrepreneurship Training Program(201710601082)
文摘[Objectives] To optimize the extraction process of total flavonoids in stems of Mallotus apelta. [Methods]On the basis of singlefactor test,with volume fraction of ethanol,extraction time and ratio of solvent as independent variables,the content of total flavonoids as dependent variables,the completely secondary response surface regression fitting was conducted on the independent and dependent variables,and the Response Surface Method was used to optimize the optimum extraction process of total flavonoids in Mallotus apelta stems and predict the optimum process. [Results] The optimum extraction process of total flavonoids in Mallotus apelta was determined as follows: ethanol concentration of 71. 5%; extraction time of 154. 6 min; solid-liquid ratio of 1∶19. 2; total flavonoids content of 7. 060 mg/g; fitted binomial squared correlation coefficient R^2= 0. 8751.[Conclusions]Composite Design/Response Surface Method could be used in the extraction process optimization of total flavonoids in Mallotus apelta stems,the mathematical model established had high prediction accuracy,the method was simple and operability was good.
基金Supported by Guilin Science and Technology Bureau Project(20100305)Guangxi"2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(G2013[20])
文摘[Objectives] This study was conducted to determine kaempferol content in ginkgo( Ginkgo biloba L.) leaves subjected to microbial fermentation.[Methods]Bacillus licheniformis was selected for solid-state fermentation of ginkgo leaves,and the content of kaempferol in ginkgo leaves was determined by RPHPLC method. At first,methanol was used to extract flavonoid glycosides,which were then hydrolyzed by hydrochloric acid solution. HPLC was performed with Platisil ODS column C18( 150 mm ×4. 6 mm,5 μm) using mobile phase Vmethanol∶ Vwater( 0. 4% phosphoric acid solution) = 55∶45 at a flow rate of 1 ml/min,and the eluate was detected with a shimadzu HPLC ultraviolet detector at 360 nm. [Results]With kaempferol as the reference substance,the correlation coefficient was0. 999 2 in the range of 0. 001 06-0. 016 96 g/L. The content in the fermented product was less than that in the non-fermented product by 28%. [Conclusions]The method is simple,accurate,and is suitable for determination of kaempferol. This study will provide an experimental basis for the development and utilization of ginkgo.
基金Supported by Guilin Science and Technology Bureau Project(20100305)Guangxi "2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(G2013[20])
文摘[Objectives]This study was conducted to determine the content of total flavonoids in ginkgo ( Ginkgo biloba L.) leaves.[Methods]The content of total flavonoids from ginkgo leaves was determined by reversed phase-high performance liquid chromatography (RP-HPLC).The flavonoid glycosides were first extracted with methanol,and hydrolyzed with hydrochloric acid solution to prepare a test solution.Platisil ODS C18 column (150 mm×4.6 mm,5 μm) and the mobile phase V methanol ∶ V water (0.4% phosphoric acid solution)=85∶ 15 were selected for HPLC separation.The HPLC separation was performed with the column at a column temperature of 25℃ using the mobile phase at a flow rate of 1 ml/min.The sample size was 10 μl,and detection was performed with an Agilent HPLC ultraviolet detector at 360 nm.[Results]The reference substance,quercetin,had good linearity in the range of 0.002 6-0.052 0 g/L,with a correlation coefficient of 0.999 7;and the RSD was 1.26%.[Conclusions]The determination method has rapid and simple operation with accurate results and is good in repeatability.This method is suitable for the determination of content of total flavonoids in ginkgo leaves.
基金Supported by National Natural Science Foundation of China(32060228)Guangxi Natural Science Foundation(2017GXNSFAA198112+5 种基金2019GXNSFAA245077)Guangxi Graduate Education Innovation Project(GJY2018116YCSW2019214YCSW2020225)Undergraduate Innovation Training Program(202010601164202010601093)。
文摘[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy.
