Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal application. Here, we report the systema...Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal application. Here, we report the systematic synthesis of sub-50 nm carbon nanoparticles （CNP） presenting neutral, anionic, and cationic surface functionalities. A subset of CNPs with -10, 20, and 40 nm hydrodynamic sizes were synthesized with neutral surface headgroups. For the first time, the cellular internalization of these CNPs was systematically quantified in various stages of breast cancer cells （early, late, and metastatic）, thereby providing a parametric assessment of charge and size effects. Distinct activities were observed when these systems interacted with cancer cells in various stages. Our results indicated that metastatic breast cancer could be targeted by a nanosystem presenting anionic phosphate groups. On the contrary, for patients in late stage of cancer, drugs could be delivered with sulfonate functionalized carbon nano- particles, which have higher probability of intracellular transport. This study will facilitate the better understanding of nanoparticle-biological entity interaction, and the integration of this knowledge with pathophysiology would promote the engineering of nanomedicine with superior likelihoods of crossing the endocytic ＂barrier＂ for drug delivery inside cancerous cells.展开更多
Imaging sentinel lymph nodes （SLN） could provide us with critical information about the progression of a cancerous disease. Real-time high-resolution intrao- perative photoacoustic imaging （PAI） in conjunction wit...Imaging sentinel lymph nodes （SLN） could provide us with critical information about the progression of a cancerous disease. Real-time high-resolution intrao- perative photoacoustic imaging （PAI） in conjunction with a near-infrared （NIR） probe may offer opportunities for the immediate imaging for direct identification and resection of SLN or collecting tissue samples. In this work a commercially amenable synthetic methodology is revealed for fabricating luminescent carbon nanoparticles with rapid clearance properties. A one-pot ＂green＂ technique is pursued, which involved rapid surface passivation of carbon nanopartides with organic macromolecules （e.g., polysorbate, polyethyleneglycol） in solvent-free conditions. Interestingly, the naked carbon nanoparticles are derived for the first time, from commercial food grade honey. Surface coated particles are markedly smaller （-7 nm） than previously explored particles （gold, single-walled carbon nanotubes, copper） for SLN imaging. The results indicate an exceptionally rapid signal enhancement （-2 min） of the SLN. Owing to their strong optical absorption in the NIR region, tiny size and rapid lymphatic transport, this platform offers great potential for faster resection of SLN and may lower complications caused in axillary investigation by mismarking with dyes or low-resolution imaging techniques.展开更多
Of late, many synthesis processes have been studied to develop irregular nano-rnorphologies of gold nanostructures for biomedical applications in order to increase the efficacy of nanoparticle theranostics, tune the p...Of late, many synthesis processes have been studied to develop irregular nano-rnorphologies of gold nanostructures for biomedical applications in order to increase the efficacy of nanoparticle theranostics, tune the plasmonic absorbance spectra, and increase the sensitivity of biomolecule detection through surface enhanced Raman spectroscopy. Here we report, a novel, non-seed mediated versatile single pot synthesis method capable of producing hyperbranched gold ＂nano-polyvilli＂ with more than 50-90 branching nanowires propagating from a single origin within each structure. The technique was capable of achieving precise tuning of the branch propagation where the branching could be controlled by varying the duration of incubation, temperature, and hydrogen ion concentration.展开更多
文摘Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal application. Here, we report the systematic synthesis of sub-50 nm carbon nanoparticles （CNP） presenting neutral, anionic, and cationic surface functionalities. A subset of CNPs with -10, 20, and 40 nm hydrodynamic sizes were synthesized with neutral surface headgroups. For the first time, the cellular internalization of these CNPs was systematically quantified in various stages of breast cancer cells （early, late, and metastatic）, thereby providing a parametric assessment of charge and size effects. Distinct activities were observed when these systems interacted with cancer cells in various stages. Our results indicated that metastatic breast cancer could be targeted by a nanosystem presenting anionic phosphate groups. On the contrary, for patients in late stage of cancer, drugs could be delivered with sulfonate functionalized carbon nano- particles, which have higher probability of intracellular transport. This study will facilitate the better understanding of nanoparticle-biological entity interaction, and the integration of this knowledge with pathophysiology would promote the engineering of nanomedicine with superior likelihoods of crossing the endocytic ＂barrier＂ for drug delivery inside cancerous cells.
文摘Imaging sentinel lymph nodes （SLN） could provide us with critical information about the progression of a cancerous disease. Real-time high-resolution intrao- perative photoacoustic imaging （PAI） in conjunction with a near-infrared （NIR） probe may offer opportunities for the immediate imaging for direct identification and resection of SLN or collecting tissue samples. In this work a commercially amenable synthetic methodology is revealed for fabricating luminescent carbon nanoparticles with rapid clearance properties. A one-pot ＂green＂ technique is pursued, which involved rapid surface passivation of carbon nanopartides with organic macromolecules （e.g., polysorbate, polyethyleneglycol） in solvent-free conditions. Interestingly, the naked carbon nanoparticles are derived for the first time, from commercial food grade honey. Surface coated particles are markedly smaller （-7 nm） than previously explored particles （gold, single-walled carbon nanotubes, copper） for SLN imaging. The results indicate an exceptionally rapid signal enhancement （-2 min） of the SLN. Owing to their strong optical absorption in the NIR region, tiny size and rapid lymphatic transport, this platform offers great potential for faster resection of SLN and may lower complications caused in axillary investigation by mismarking with dyes or low-resolution imaging techniques.
文摘Of late, many synthesis processes have been studied to develop irregular nano-rnorphologies of gold nanostructures for biomedical applications in order to increase the efficacy of nanoparticle theranostics, tune the plasmonic absorbance spectra, and increase the sensitivity of biomolecule detection through surface enhanced Raman spectroscopy. Here we report, a novel, non-seed mediated versatile single pot synthesis method capable of producing hyperbranched gold ＂nano-polyvilli＂ with more than 50-90 branching nanowires propagating from a single origin within each structure. The technique was capable of achieving precise tuning of the branch propagation where the branching could be controlled by varying the duration of incubation, temperature, and hydrogen ion concentration.
