Atmospheric pressure plasma-liquid interactions exist in a variety of applications,including wastewater treatment,wound sterilization,and disinfection.In practice,the phenomenon of liquid surface depression will inevi...Atmospheric pressure plasma-liquid interactions exist in a variety of applications,including wastewater treatment,wound sterilization,and disinfection.In practice,the phenomenon of liquid surface depression will inevitably appear.The applied gas will cause a depression on the liquid surface,which will undoubtedly affect the plasma generation and further affect the application performance.However,the effect of liquid surface deformation on the plasma is still unclear.In this work,numerical models are developed to reveal the mechanism of liquid surface depressions affecting plasma discharge characteristics and the consequential distribution of plasma species,and further study the influence of liquid surface depressions of different sizes generated by different helium flow rates on the plasma.Results show that the liquid surface deformation changes the initial spatial electric field,resulting in the rearrangement of electrons on the liquid surface.The charges deposited on the liquid surface further increase the degree of distortion of the electric field.Moreover,the electric field and electron distribution affected by the liquid surface depression significantly influence the generation and distribution of active species,which determines the practical effectiveness of the relevant applications.This work explores the phenomenon of liquid surface depression,which has been neglected in previous related work,and contributes to further understanding of plasma-liquid interactions,providing better theoretical guidance for related applications and technologies.展开更多
Secondary electron emission(SEE)induced by the positive ion is an essential physical process to influence the dynamics of gas discharge which relies on the specific surface material.Surface charging has a significant ...Secondary electron emission(SEE)induced by the positive ion is an essential physical process to influence the dynamics of gas discharge which relies on the specific surface material.Surface charging has a significant impact on the material properties,thereby affecting the SEE in the plasma-surface interactions.However,it does not attract enough attention in the previous studies.In this paper,SEE dependent on the charged surface of specific materials is described with the computational method combining a density functional theory(DFT)model from the first-principle theory and the theory of Auger neutralization.The effect ofκ-Al2O3 surface charge,as an example,on the ion-induced secondary electron emission coefficient(SEEC)is investigated by analyzing the defect energy level and band structure on the charged surface.Simulation results indicate that,with the surface charge from negative to positive,the SEEC of a part of low ionization energy ions(such as Ei=12.6 eV)increases first and then decreases,exhibiting a nonlinear changing trend.This is quite different from the monotonic decreasing tendency observed in the previous model which simplifies the electronic structure.This irregular increase of the SEEC can be attributed to the lower escaped probability of orbital energy.The results further illustrate that the excessive charge could cause the bottom of the conduction band close to the valence band,thus leading to the decrease of the orbital energy occupied by the excited electrons.The nonlinear change of SEEC demonstrates a more realistic situation of how the electronic structure of material surface influences the SEE process.This work provides an accurate method of calculating SEEC from specific materials,which is urgent in widespread physical scenarios sensitive to surface materials,such as increasingly growing practical applications concerning plasma-surface interactions.展开更多
Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed a...Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed addi-tives for their ability to improve growth performance and intestinal health in poultry.However,whether dietary OA can ameliorate HSD stress-induced impaired intestinal barrier in broilers remains elusive.In this study,a total of 528 one-day-old male Arbor Acres broilers were randomly allocated into 3 treatments with 12 replicates per treatment including 10 birds for normal stocking density and 17 birds for HSD.The dietary treatments were as follows:1)Normal stocking density+basal diet;2)HSD+basal diets;3)HSD+OA.Results:HSD stress can induce increased levels of serum corticosterone,lipopolysaccharides,interleukin-1β,tumor necrosis factor-α,and down-regulated mRNA expression of ZO-1,resulting in compromised growth performance of broilers(P<0.05).Dietary OA could significantly reduce levels of serum corticosterone,lipopolysaccharides,interleukin-1β,and tumor necrosis factor-α,which were accompanied by up-regulated interleukin-10,mRNA expres-sion of ZO-1,and growth performance(P<0.05).Moreover,OA could down-regulate the mRNA expression of TLR4 and MyD88 to inhibit the NF-κB signaling pathway(P<0.05).Additionally,HSD stress significantly decreased the abundance of Bacteroidetes and disturbed the balance of microbial ecosystems,whereas OA significantly increased the abundance of Bacteroidetes and restored the disordered gut microbiota by reducing competitive and exploita-tive interactions in microbial communities(P<0.05).Meanwhile,OA significantly increased the content of acetic and butyric acids,which showed significant correlations with intestinal inflammation indicators(P<0.05).Conclusions:Dietary OA ameliorated intestinal inflammation and growth performance of broilers through restor-ing the disordered gut microbial compositions and interactions induced by HSD and elevating short-chain fatty acid production to inhibit the TLR4/NF-κB signaling pathway.These findings demonstrated the critical role of intestinal microbiota in mediating the HSD-induced inflammatory responses,contributing to exploring nutritional strategies to alleviate HSD-induced stress in animals.展开更多
AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mic...AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the survival rate of acute liver failure(ALF)mice(73.3%vs 20.0%,P<0.0001),and SP600125 could inhibit the effect of DHM.CONCLUSION:These findings demonstrate that DHM alleviates CCl4-induced liver injury,suggesting that DHM is a promising candidate for reversing liver injury and ALF.展开更多
Background:Prenatal nutrition is crucial for embryonic development and neonatal growth,and has the potential to be a main determinant of life-long health.In the present study,we used a layer chick model to investigate...Background:Prenatal nutrition is crucial for embryonic development and neonatal growth,and has the potential to be a main determinant of life-long health.In the present study,we used a layer chick model to investigate the effects of in ovo feeding(IOF)of L-arginine(Arg)on growth,intestinal development,intestinal microbiota and metabolism.The treatments included the non-injected control,saline-injected control,and saline containing 2,6,or 10 mg Arg groups.Results:IOF Arg increased early intestinal index and villus height,and enhanced uptake of residual yolk lipid,contributing to subsequent improvement in the early growth performance of chicks.Prenatal Arg supplementation also increased the early microbialα-diversity,the relative abundance of Lactobacillales and Clostridiales,and decreased the relative abundance of Proteobacteria of cecum in chicks.Furthermore,the shift of cecal microbiota composition and the colonization of potential probiotics were accelerated by IOF of Arg.Simultaneously,metabolomics showed that metabolisms of galactose,taurine-conjugated bile acids and lipids were modulated to direct more energy and nutrients towards rapid growth of intestine at the beginning of post-hatch when embryos received IOF of Arg.Conclusions:Prenatal Arg supplementation showed beneficial effects on the early intestinal development,cecal microbiota and host metabolism of layer chicks,contributing to subsequent improvement in the early growth performance.These findings provide new insight into the role of IOF of Arg in the establishment of the gut microbiota of newly-hatched layer chicks,and can expand our fundamental knowledge about prenatal nutrition,early bacterial colonization and intestinal development in neonate.展开更多
AIM To compare ^(18)F-fluorodeoxyglucose positron emission tomography/computed tomography(^(18)F-FDG PET/CT) features in gastric lymphoma and gastric carcinoma.METHODS Patients with newly diagnosed gastric lymphoma or...AIM To compare ^(18)F-fluorodeoxyglucose positron emission tomography/computed tomography(^(18)F-FDG PET/CT) features in gastric lymphoma and gastric carcinoma.METHODS Patients with newly diagnosed gastric lymphoma or gastric carcinoma who underwent ^(18)F-FDG PET/CT prior to treatment were included in this study. We reviewed and analyzed the PET/CT features of gastric wall lesions,including FDG avidity,pattern(focal/diffuse),and intensity [maximal standard uptake value:(SUVmax)]. The correlation of SUVmax with gastricclinicopathological variables was investigated by χ~2 test,and receiver-operating characteristic(ROC) curve analysis was performed to determine the differential diagnostic value of SUVmax-associated parameters in gastric lymphoma and gastric carcinoma. RESULTS Fifty-two patients with gastric lymphoma and 73 with gastric carcinoma were included in this study. Abnormal gastric FDG accumulation was found in 49 patients(94.23%) with gastric lymphoma and 65 patients(89.04%) with gastric carcinoma. Gastric lymphoma patients predominantly presented with type Ⅰ and type Ⅱ lesions,whereas gastric carcinoma patients mainly had type Ⅲ lesions. The SUVmax(13.39 ± 9.24 vs 8.35 ± 5.80,P < 0.001) and SUVmax/THKmax(maximal thickness)(7.96 ± 4.02 vs 4.88 ± 3.32,P < 0.001) were both higher in patients with gastric lymphoma compared with gastric carcinoma. ROC curve analysis suggested a better performance of SUVmax/THKmax in the evaluation of gastric lesions between gastric lymphoma and gastric carcinoma in comparison with that of SUVmax alone.CONCLUSION PET/CT features differ between gastric lymphoma and carcinoma,which can improve PET/CT evaluation of gastric wall lesions and help differentiate gastric lymphoma from gastric carcinoma.展开更多
In this paper, a two-dimensional axisymmetric fluid model was established to investigate the influence of nitrogen impurity content on the discharge pattern and the relevant discharge characteristics in an atmosphere ...In this paper, a two-dimensional axisymmetric fluid model was established to investigate the influence of nitrogen impurity content on the discharge pattern and the relevant discharge characteristics in an atmosphere pressure helium dielectric barrier discharge(DBD). The results indicated that when the nitrogen content was increased from 1 to 100 ppm, the discharge pattern evolved from a concentric-ring pattern into a uniform pattern, and then returned to the concentricring pattern. In this process, the discharge mode at the current peak moment transformed from glow mode into Townsend mode, and then returned to glow mode. Further analyses revealed that with the increase of impurity level, the rate of Penning ionization at the pre-ionization stage increased at first and decreased afterwards, resulting in a similar evolution pattern of seed electron level. This evolution trend was believed to be resulted from the competition between the N2 partial pressure and the consumption rate of metastable species. Moreover, the discharge uniformity was found positively correlated with the spatial uniformity of seed electron density as well as the seed electron level. The reason for this correlation was explained by the reduction of radial electric field strength and the promotion of seed electron uniformity as pre-ionization level increases. The results obtained in this work may help better understand the pattern formation mechanism of atmospheric helium DBD under the variation of N2 impurity level, thereby providing a possible means of regulating the discharge performance in practical application scenarios.展开更多
Parkinson’s disease(PD)is a very common neurodegenerative disease that occurs mostly in the elderly.There are many main clinical manifestations of PD,such as tremor,bradykinesia,muscle rigidity,etc.Based on the curre...Parkinson’s disease(PD)is a very common neurodegenerative disease that occurs mostly in the elderly.There are many main clinical manifestations of PD,such as tremor,bradykinesia,muscle rigidity,etc.Based on the current research on PD,the accurate and convenient detection of early symptoms is the key to detect PD.With the development of microelectronic and sensor technology,it is much easier to measure the barely noticeable tremor in just one hand for the early detection of Parkinson’s disease.In this paper,we present a smart wearable device for detecting hand tremor,in which MPU6050(MIDI Processing Unit)consisting of a 3-axis gyroscope and a 3-axis accelerometer is used to collect acceleration and angular velocity of fingers.By analyzing the time of specific finger movements,we successfully recognized the tremor signals with high accuracy.Meanwhile,with Bluetooth 4.0(Bluetooth Low Energy,BLE)and networking terminal ability,tremor data can be transferred to a monitoring device in real time with extremely lowenergy consumption.The experimental results have shown that the proposed device(smart ring)is convenient for long-term tremor detection which is vital for early detection and treatment for Parkinson’s disease.展开更多
High-pressure solenoid valve with high flow rate and high speed is a key component in an underwater driving system.However,traditional single spool pilot operated valve cannot meet the demands of both high flow rate a...High-pressure solenoid valve with high flow rate and high speed is a key component in an underwater driving system.However,traditional single spool pilot operated valve cannot meet the demands of both high flow rate and high speed simultaneously.A new structure for a high pressure solenoid valve is needed to meet the demand of the underwater driving system.A novel parallel-spool pilot operated high-pressure solenoid valve is proposed to overcome the drawback of the current single spool design.Mathematical models of the opening process and flow rate of the valve are established.Opening response time of the valve is subdivided into 4 parts to analyze the properties of the opening response.Corresponding formulas to solve 4 parts of the response time are derived.Key factors that influence the opening response time are analyzed.According to the mathematical model of the valve,a simulation of the opening process is carried out by MATLAB.Parameters are chosen based on theoretical analysis to design the test prototype of the new type of valve.Opening response time of the designed valve is tested by verifying response of the current in the coil and displacement of the main valve spool.The experimental results are in agreement with the simulated results,therefore the validity of the theoretical analysis is verified.Experimental opening response time of the valve is 48.3 ms at working pressure of 10 MPa.The flow capacity test shows that the largest effective area is 126 mm2 and the largest air flow rate is 2320 L/s.According to the result of the load driving test,the valve can meet the demands of the driving system.The proposed valve with parallel spools provides a new method for the design of a high-pressure valve with fast response and large flow rate.展开更多
Atmospheric dielectric barrier discharges driven by repetitive unipolar narrow pulse excitation are investigated numerically by using one-dimensional fluid models.The one-dimensional simulation focuses on the effects ...Atmospheric dielectric barrier discharges driven by repetitive unipolar narrow pulse excitation are investigated numerically by using one-dimensional fluid models.The one-dimensional simulation focuses on the effects of applied voltage amplitude,pulse repetition frequency,gap width andγcoefficient on the multiple-current-pulse(MCP)discharge.The results indicate that the MCP behavior will lead to the stratification of electron density distribution in axial direction.Traditional MCP manipulating methods,such as reducing the applied voltage amplitude,increasing the applied voltage frequency,adjusting the gap width,cannot regulate MCPs exhibiting in this work.Further analyses reveal that the increasing electric field of the cathode fall region is the basis for the emergence of MCP behavior.展开更多
The nonlinear phenomenon is very popular in dielectric barrier discharge （DBD） plasmas. There are at least three kinds of spatial and temporal nonlinear phenomena appearing synchronously or asynchronously in DBDs, i...The nonlinear phenomenon is very popular in dielectric barrier discharge （DBD） plasmas. There are at least three kinds of spatial and temporal nonlinear phenomena appearing synchronously or asynchronously in DBDs, i.e. self-organized patterns, striations and chaos. This paper describes the recent research and progress in understanding the nature of these nonlinear phenomena. Patterns are macroscopic structures with certain spatial and/or temporal periodicities generated through self- organization of microscopic parameters. The physics of patterns in DBDs is mainly associated with lateral dynamic behaviors or the lateral non-local effect of charged particles resulting in the lateral development or non-uniformity of discharge. Striations are ionization waves with unique properties determined by transport phenomena, ionization processes and electron kinetics in current-carrying plasmas. The physics of striations in DBDs is mainly associated with the advances in non-local electron kinetics in spatially inhomogeneous plasmas. Chaos is a kind of random and non-periodic phenomenon occunfng in a determined dynamic system, following a series of certain rules while exhibiting random locomotion, and is regarded as an intrinsic and ubiquitous phenomenon in a nonlinear dynamic system. An evolution trajectory including period-doubling bifurcation to chaos was observed in DBDs or DBD-derived plasmas. In a common sense, it is believed that the formation of all the three nonlinear phenomena in a DBD system should be related to the non-local transversal and/or longitudinal dynamics of space charges （i.e. non-local effect） or the localized electric field interaction. Future work is still needed on the underlying physics and should be directed to pursuing the unification of these nonlinear phenomena in DBD.展开更多
Dielectric barrier discharges(DBD)are widely utilised non‐equilibrium atmospheric pressure plasmas with a diverse range of applications,such as material processing,surface treatment,light sources,pollution control,an...Dielectric barrier discharges(DBD)are widely utilised non‐equilibrium atmospheric pressure plasmas with a diverse range of applications,such as material processing,surface treatment,light sources,pollution control,and medicine.Over the course of several decades,extensive research has been dedicated to the generation of homogeneous DBD(H‐DBD),focussing on understanding the transition from H‐DBD to filamentary DBD and exploring strategies to create and sustain H‐DBD.This paper first discusses the in-fluence of various parameters on DBD,including gas flow,dielectric material,surface conductivity,and mesh electrode.Secondly,a chronological literature review is presented,highlighting the development of H‐DBD and the associated understanding of its un-derlying mechanisms.This encompasses the generation of H‐DBD in helium,nitrogen,and air.Lastly,the paper provides a brief overview of multiple‐current‐pulse(MCP)behaviours in H‐DBD.The objective of this article is to provide a chronological un-derstanding of homogeneous dielectric barrier discharge(DBD).This understanding will aid in the design of new experiments aimed at better comprehending the mechanisms behind H‐DBD generation and ultimately assist in achieving large‐volume H‐DBD in an air environment.展开更多
The real-time monitoring of the load in farming by the sensor installed on the tractor's three-point hitch can effectively improve the farming efficiency and force-position combined control,reduce the compaction r...The real-time monitoring of the load in farming by the sensor installed on the tractor's three-point hitch can effectively improve the farming efficiency and force-position combined control,reduce the compaction risk of the wheel on the soil and reduce the fuel consumption in farming process.However,the measurement and quantification of the loads on the three-point hitch have some problems remaining unresolved:testing the accuracy and reliability of a load measuring system is hard when the tractor works in a field,the mathematical model of spatial forces usually lacks a practical and effective validation,and the calibration process of the measurement system is inconvenient and incomplete while easily causing a low accuracy.Specifically,this paper builds a new spatial-force mathematical model based on the geometry of a three-point hitch.To eliminate the discrepancy of the geometric model with the actual structure and to refine the mathematical model,a calibration process is conducted by developing a calibration bench,which is equipped with a data acquisition system and a multi-parameter monitoring interface.The three-point hitch installed on this calibration bench is subject to steady-state loading.The loading force,angle of the lower drawbar,and three-component forces(three shaft pin sensors’forces)of the three-point hitch are well measured.With applying for the measured data to calibrate the theoretical mathematic model eventually derives the resultant force from all the three-component forces,a dynamical loading bench was developed to test the calculated resultant force for the three-point hitch during the sinusoidal and randomly variant dynamical loadings tests.A hitch force measurement system is also developed to collect real-time data and calculate the resultant force of measured three-component forces through the calibrated mathematical model.The results of the dynamical loading tests show that the average relative error MRE=1.09%with an average force measurement time delay beingΔt=0.5 s,the root mean square error RMSE=59.3 N,and the coefficient of determination R2=0.9903.As observed,the shape and the trend of the generated resultant force curve are basically the dynamical loading force.The dynamical loading test proves the high efficacy and reliability of the proposed indoor calibration method for calculating the load based on the three-component forces as measured on the three-point hitch.Besides,the preliminary study of the proposed method on the hitch load provides great potential to improve the indoor six-component measurement and quantification of both the force and momentum acting on the three-point hitch.展开更多
基金supported by National Natural Science Foundation of China(No.52377145).
文摘Atmospheric pressure plasma-liquid interactions exist in a variety of applications,including wastewater treatment,wound sterilization,and disinfection.In practice,the phenomenon of liquid surface depression will inevitably appear.The applied gas will cause a depression on the liquid surface,which will undoubtedly affect the plasma generation and further affect the application performance.However,the effect of liquid surface deformation on the plasma is still unclear.In this work,numerical models are developed to reveal the mechanism of liquid surface depressions affecting plasma discharge characteristics and the consequential distribution of plasma species,and further study the influence of liquid surface depressions of different sizes generated by different helium flow rates on the plasma.Results show that the liquid surface deformation changes the initial spatial electric field,resulting in the rearrangement of electrons on the liquid surface.The charges deposited on the liquid surface further increase the degree of distortion of the electric field.Moreover,the electric field and electron distribution affected by the liquid surface depression significantly influence the generation and distribution of active species,which determines the practical effectiveness of the relevant applications.This work explores the phenomenon of liquid surface depression,which has been neglected in previous related work,and contributes to further understanding of plasma-liquid interactions,providing better theoretical guidance for related applications and technologies.
基金supported by the National Key Research and Development Plan of China(No.2021YFE0114700)National Natural Science Foundation of China(No.52377145).
文摘Secondary electron emission(SEE)induced by the positive ion is an essential physical process to influence the dynamics of gas discharge which relies on the specific surface material.Surface charging has a significant impact on the material properties,thereby affecting the SEE in the plasma-surface interactions.However,it does not attract enough attention in the previous studies.In this paper,SEE dependent on the charged surface of specific materials is described with the computational method combining a density functional theory(DFT)model from the first-principle theory and the theory of Auger neutralization.The effect ofκ-Al2O3 surface charge,as an example,on the ion-induced secondary electron emission coefficient(SEEC)is investigated by analyzing the defect energy level and band structure on the charged surface.Simulation results indicate that,with the surface charge from negative to positive,the SEEC of a part of low ionization energy ions(such as Ei=12.6 eV)increases first and then decreases,exhibiting a nonlinear changing trend.This is quite different from the monotonic decreasing tendency observed in the previous model which simplifies the electronic structure.This irregular increase of the SEEC can be attributed to the lower escaped probability of orbital energy.The results further illustrate that the excessive charge could cause the bottom of the conduction band close to the valence band,thus leading to the decrease of the orbital energy occupied by the excited electrons.The nonlinear change of SEEC demonstrates a more realistic situation of how the electronic structure of material surface influences the SEE process.This work provides an accurate method of calculating SEEC from specific materials,which is urgent in widespread physical scenarios sensitive to surface materials,such as increasingly growing practical applications concerning plasma-surface interactions.
基金supported by the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences,and Trouw Nutrition Research&Development Centers.
文摘Background:High stocking density(HSD)stress has detrimental effects on growth performance,intestinal barrier function,and intestinal microbiota in intensive animal production.Organic acids(OA)are widely used as feed addi-tives for their ability to improve growth performance and intestinal health in poultry.However,whether dietary OA can ameliorate HSD stress-induced impaired intestinal barrier in broilers remains elusive.In this study,a total of 528 one-day-old male Arbor Acres broilers were randomly allocated into 3 treatments with 12 replicates per treatment including 10 birds for normal stocking density and 17 birds for HSD.The dietary treatments were as follows:1)Normal stocking density+basal diet;2)HSD+basal diets;3)HSD+OA.Results:HSD stress can induce increased levels of serum corticosterone,lipopolysaccharides,interleukin-1β,tumor necrosis factor-α,and down-regulated mRNA expression of ZO-1,resulting in compromised growth performance of broilers(P<0.05).Dietary OA could significantly reduce levels of serum corticosterone,lipopolysaccharides,interleukin-1β,and tumor necrosis factor-α,which were accompanied by up-regulated interleukin-10,mRNA expres-sion of ZO-1,and growth performance(P<0.05).Moreover,OA could down-regulate the mRNA expression of TLR4 and MyD88 to inhibit the NF-κB signaling pathway(P<0.05).Additionally,HSD stress significantly decreased the abundance of Bacteroidetes and disturbed the balance of microbial ecosystems,whereas OA significantly increased the abundance of Bacteroidetes and restored the disordered gut microbiota by reducing competitive and exploita-tive interactions in microbial communities(P<0.05).Meanwhile,OA significantly increased the content of acetic and butyric acids,which showed significant correlations with intestinal inflammation indicators(P<0.05).Conclusions:Dietary OA ameliorated intestinal inflammation and growth performance of broilers through restor-ing the disordered gut microbial compositions and interactions induced by HSD and elevating short-chain fatty acid production to inhibit the TLR4/NF-κB signaling pathway.These findings demonstrated the critical role of intestinal microbiota in mediating the HSD-induced inflammatory responses,contributing to exploring nutritional strategies to alleviate HSD-induced stress in animals.
基金Supported by Initial Fund of Guangdong Medical College,No.XB1338the Medical Research Fund of Guangdong Province,No.B2014306the Research Fund of Guangdong Medical College,No.M2013024
文摘AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the survival rate of acute liver failure(ALF)mice(73.3%vs 20.0%,P<0.0001),and SP600125 could inhibit the effect of DHM.CONCLUSION:These findings demonstrate that DHM alleviates CCl4-induced liver injury,suggesting that DHM is a promising candidate for reversing liver injury and ALF.
基金This study was supported by National Key R&D Program of China(2017YFD0500500)the earmarked fund for Modern Agro-industry Technology Research System(CARS-40-K12)+1 种基金Beijing Innovation Consortium of Agriculture Research System(BAIC04–2018)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences.
文摘Background:Prenatal nutrition is crucial for embryonic development and neonatal growth,and has the potential to be a main determinant of life-long health.In the present study,we used a layer chick model to investigate the effects of in ovo feeding(IOF)of L-arginine(Arg)on growth,intestinal development,intestinal microbiota and metabolism.The treatments included the non-injected control,saline-injected control,and saline containing 2,6,or 10 mg Arg groups.Results:IOF Arg increased early intestinal index and villus height,and enhanced uptake of residual yolk lipid,contributing to subsequent improvement in the early growth performance of chicks.Prenatal Arg supplementation also increased the early microbialα-diversity,the relative abundance of Lactobacillales and Clostridiales,and decreased the relative abundance of Proteobacteria of cecum in chicks.Furthermore,the shift of cecal microbiota composition and the colonization of potential probiotics were accelerated by IOF of Arg.Simultaneously,metabolomics showed that metabolisms of galactose,taurine-conjugated bile acids and lipids were modulated to direct more energy and nutrients towards rapid growth of intestine at the beginning of post-hatch when embryos received IOF of Arg.Conclusions:Prenatal Arg supplementation showed beneficial effects on the early intestinal development,cecal microbiota and host metabolism of layer chicks,contributing to subsequent improvement in the early growth performance.These findings provide new insight into the role of IOF of Arg in the establishment of the gut microbiota of newly-hatched layer chicks,and can expand our fundamental knowledge about prenatal nutrition,early bacterial colonization and intestinal development in neonate.
基金Supported by the National Natural Science Foundation of China,No.81501984 and No.81601377Tianjin Municipal Bureau of Health Science and Technology,No.2015KZ084 and No.2013KZ088Tianjin Medical University Science,No.2013KYQ07
文摘AIM To compare ^(18)F-fluorodeoxyglucose positron emission tomography/computed tomography(^(18)F-FDG PET/CT) features in gastric lymphoma and gastric carcinoma.METHODS Patients with newly diagnosed gastric lymphoma or gastric carcinoma who underwent ^(18)F-FDG PET/CT prior to treatment were included in this study. We reviewed and analyzed the PET/CT features of gastric wall lesions,including FDG avidity,pattern(focal/diffuse),and intensity [maximal standard uptake value:(SUVmax)]. The correlation of SUVmax with gastricclinicopathological variables was investigated by χ~2 test,and receiver-operating characteristic(ROC) curve analysis was performed to determine the differential diagnostic value of SUVmax-associated parameters in gastric lymphoma and gastric carcinoma. RESULTS Fifty-two patients with gastric lymphoma and 73 with gastric carcinoma were included in this study. Abnormal gastric FDG accumulation was found in 49 patients(94.23%) with gastric lymphoma and 65 patients(89.04%) with gastric carcinoma. Gastric lymphoma patients predominantly presented with type Ⅰ and type Ⅱ lesions,whereas gastric carcinoma patients mainly had type Ⅲ lesions. The SUVmax(13.39 ± 9.24 vs 8.35 ± 5.80,P < 0.001) and SUVmax/THKmax(maximal thickness)(7.96 ± 4.02 vs 4.88 ± 3.32,P < 0.001) were both higher in patients with gastric lymphoma compared with gastric carcinoma. ROC curve analysis suggested a better performance of SUVmax/THKmax in the evaluation of gastric lesions between gastric lymphoma and gastric carcinoma in comparison with that of SUVmax alone.CONCLUSION PET/CT features differ between gastric lymphoma and carcinoma,which can improve PET/CT evaluation of gastric wall lesions and help differentiate gastric lymphoma from gastric carcinoma.
基金supported by National Natural Science Foundation of China (Grant No. 51877086)
文摘In this paper, a two-dimensional axisymmetric fluid model was established to investigate the influence of nitrogen impurity content on the discharge pattern and the relevant discharge characteristics in an atmosphere pressure helium dielectric barrier discharge(DBD). The results indicated that when the nitrogen content was increased from 1 to 100 ppm, the discharge pattern evolved from a concentric-ring pattern into a uniform pattern, and then returned to the concentricring pattern. In this process, the discharge mode at the current peak moment transformed from glow mode into Townsend mode, and then returned to glow mode. Further analyses revealed that with the increase of impurity level, the rate of Penning ionization at the pre-ionization stage increased at first and decreased afterwards, resulting in a similar evolution pattern of seed electron level. This evolution trend was believed to be resulted from the competition between the N2 partial pressure and the consumption rate of metastable species. Moreover, the discharge uniformity was found positively correlated with the spatial uniformity of seed electron density as well as the seed electron level. The reason for this correlation was explained by the reduction of radial electric field strength and the promotion of seed electron uniformity as pre-ionization level increases. The results obtained in this work may help better understand the pattern formation mechanism of atmospheric helium DBD under the variation of N2 impurity level, thereby providing a possible means of regulating the discharge performance in practical application scenarios.
基金supported by the National Natural Science Foundation of China(Grant Nos.61972207 and 61802196)Jiangsu Provincial Government Scholarship for Studying Abroad and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)fund.
文摘Parkinson’s disease(PD)is a very common neurodegenerative disease that occurs mostly in the elderly.There are many main clinical manifestations of PD,such as tremor,bradykinesia,muscle rigidity,etc.Based on the current research on PD,the accurate and convenient detection of early symptoms is the key to detect PD.With the development of microelectronic and sensor technology,it is much easier to measure the barely noticeable tremor in just one hand for the early detection of Parkinson’s disease.In this paper,we present a smart wearable device for detecting hand tremor,in which MPU6050(MIDI Processing Unit)consisting of a 3-axis gyroscope and a 3-axis accelerometer is used to collect acceleration and angular velocity of fingers.By analyzing the time of specific finger movements,we successfully recognized the tremor signals with high accuracy.Meanwhile,with Bluetooth 4.0(Bluetooth Low Energy,BLE)and networking terminal ability,tremor data can be transferred to a monitoring device in real time with extremely lowenergy consumption.The experimental results have shown that the proposed device(smart ring)is convenient for long-term tremor detection which is vital for early detection and treatment for Parkinson’s disease.
文摘High-pressure solenoid valve with high flow rate and high speed is a key component in an underwater driving system.However,traditional single spool pilot operated valve cannot meet the demands of both high flow rate and high speed simultaneously.A new structure for a high pressure solenoid valve is needed to meet the demand of the underwater driving system.A novel parallel-spool pilot operated high-pressure solenoid valve is proposed to overcome the drawback of the current single spool design.Mathematical models of the opening process and flow rate of the valve are established.Opening response time of the valve is subdivided into 4 parts to analyze the properties of the opening response.Corresponding formulas to solve 4 parts of the response time are derived.Key factors that influence the opening response time are analyzed.According to the mathematical model of the valve,a simulation of the opening process is carried out by MATLAB.Parameters are chosen based on theoretical analysis to design the test prototype of the new type of valve.Opening response time of the designed valve is tested by verifying response of the current in the coil and displacement of the main valve spool.The experimental results are in agreement with the simulated results,therefore the validity of the theoretical analysis is verified.Experimental opening response time of the valve is 48.3 ms at working pressure of 10 MPa.The flow capacity test shows that the largest effective area is 126 mm2 and the largest air flow rate is 2320 L/s.According to the result of the load driving test,the valve can meet the demands of the driving system.The proposed valve with parallel spools provides a new method for the design of a high-pressure valve with fast response and large flow rate.
基金supported by National Natural Science Foundation of China(No.51877086)。
文摘Atmospheric dielectric barrier discharges driven by repetitive unipolar narrow pulse excitation are investigated numerically by using one-dimensional fluid models.The one-dimensional simulation focuses on the effects of applied voltage amplitude,pulse repetition frequency,gap width andγcoefficient on the multiple-current-pulse(MCP)discharge.The results indicate that the MCP behavior will lead to the stratification of electron density distribution in axial direction.Traditional MCP manipulating methods,such as reducing the applied voltage amplitude,increasing the applied voltage frequency,adjusting the gap width,cannot regulate MCPs exhibiting in this work.Further analyses reveal that the increasing electric field of the cathode fall region is the basis for the emergence of MCP behavior.
基金National Natural Science Foundation of China for continuous financial support under Grant Nos. 10475007, 10875010, 11175017 and 51607074the State Education Ministry of China under Grant No. NCET-05-0176
文摘The nonlinear phenomenon is very popular in dielectric barrier discharge （DBD） plasmas. There are at least three kinds of spatial and temporal nonlinear phenomena appearing synchronously or asynchronously in DBDs, i.e. self-organized patterns, striations and chaos. This paper describes the recent research and progress in understanding the nature of these nonlinear phenomena. Patterns are macroscopic structures with certain spatial and/or temporal periodicities generated through self- organization of microscopic parameters. The physics of patterns in DBDs is mainly associated with lateral dynamic behaviors or the lateral non-local effect of charged particles resulting in the lateral development or non-uniformity of discharge. Striations are ionization waves with unique properties determined by transport phenomena, ionization processes and electron kinetics in current-carrying plasmas. The physics of striations in DBDs is mainly associated with the advances in non-local electron kinetics in spatially inhomogeneous plasmas. Chaos is a kind of random and non-periodic phenomenon occunfng in a determined dynamic system, following a series of certain rules while exhibiting random locomotion, and is regarded as an intrinsic and ubiquitous phenomenon in a nonlinear dynamic system. An evolution trajectory including period-doubling bifurcation to chaos was observed in DBDs or DBD-derived plasmas. In a common sense, it is believed that the formation of all the three nonlinear phenomena in a DBD system should be related to the non-local transversal and/or longitudinal dynamics of space charges （i.e. non-local effect） or the localized electric field interaction. Future work is still needed on the underlying physics and should be directed to pursuing the unification of these nonlinear phenomena in DBD.
基金supported by the National Natural Science Foundation of China(Grant Nos.52130701,51977096,and 12005076)National Key Research and Development Program of China(Grant No.2021YFE0114700)Effort of C.Jiang is supported by the Air Force Office of Scientific Research of the United States of America(Grant Nos FA9550‐22‐1‐0115 and FA9550‐22‐1‐0428).
文摘Dielectric barrier discharges(DBD)are widely utilised non‐equilibrium atmospheric pressure plasmas with a diverse range of applications,such as material processing,surface treatment,light sources,pollution control,and medicine.Over the course of several decades,extensive research has been dedicated to the generation of homogeneous DBD(H‐DBD),focussing on understanding the transition from H‐DBD to filamentary DBD and exploring strategies to create and sustain H‐DBD.This paper first discusses the in-fluence of various parameters on DBD,including gas flow,dielectric material,surface conductivity,and mesh electrode.Secondly,a chronological literature review is presented,highlighting the development of H‐DBD and the associated understanding of its un-derlying mechanisms.This encompasses the generation of H‐DBD in helium,nitrogen,and air.Lastly,the paper provides a brief overview of multiple‐current‐pulse(MCP)behaviours in H‐DBD.The objective of this article is to provide a chronological un-derstanding of homogeneous dielectric barrier discharge(DBD).This understanding will aid in the design of new experiments aimed at better comprehending the mechanisms behind H‐DBD generation and ultimately assist in achieving large‐volume H‐DBD in an air environment.
基金This work was financially supported by the project of agricultural machinery R&D,manufacturing,promotion,application,and integrationand the 13th Five-Year Plan National Key Research and Development Program of China(Grant No.2016YFD0700102).
文摘The real-time monitoring of the load in farming by the sensor installed on the tractor's three-point hitch can effectively improve the farming efficiency and force-position combined control,reduce the compaction risk of the wheel on the soil and reduce the fuel consumption in farming process.However,the measurement and quantification of the loads on the three-point hitch have some problems remaining unresolved:testing the accuracy and reliability of a load measuring system is hard when the tractor works in a field,the mathematical model of spatial forces usually lacks a practical and effective validation,and the calibration process of the measurement system is inconvenient and incomplete while easily causing a low accuracy.Specifically,this paper builds a new spatial-force mathematical model based on the geometry of a three-point hitch.To eliminate the discrepancy of the geometric model with the actual structure and to refine the mathematical model,a calibration process is conducted by developing a calibration bench,which is equipped with a data acquisition system and a multi-parameter monitoring interface.The three-point hitch installed on this calibration bench is subject to steady-state loading.The loading force,angle of the lower drawbar,and three-component forces(three shaft pin sensors’forces)of the three-point hitch are well measured.With applying for the measured data to calibrate the theoretical mathematic model eventually derives the resultant force from all the three-component forces,a dynamical loading bench was developed to test the calculated resultant force for the three-point hitch during the sinusoidal and randomly variant dynamical loadings tests.A hitch force measurement system is also developed to collect real-time data and calculate the resultant force of measured three-component forces through the calibrated mathematical model.The results of the dynamical loading tests show that the average relative error MRE=1.09%with an average force measurement time delay beingΔt=0.5 s,the root mean square error RMSE=59.3 N,and the coefficient of determination R2=0.9903.As observed,the shape and the trend of the generated resultant force curve are basically the dynamical loading force.The dynamical loading test proves the high efficacy and reliability of the proposed indoor calibration method for calculating the load based on the three-component forces as measured on the three-point hitch.Besides,the preliminary study of the proposed method on the hitch load provides great potential to improve the indoor six-component measurement and quantification of both the force and momentum acting on the three-point hitch.
