Pilomatrixomas are benign tumors derived from hair stromal cells;however,their exact etiology is unknown.A 31-year-old woman presented with a subcutaneous mass located precisely above the left eyebrow,with shedding of...Pilomatrixomas are benign tumors derived from hair stromal cells;however,their exact etiology is unknown.A 31-year-old woman presented with a subcutaneous mass located precisely above the left eyebrow,with shedding of eyebrow hair around the mass,and the shedding area gradually expanded.Surgical removal of the mass failed to prevent eyebrow loss,and the entire left eyebrow shed 6 months postoperatively.Pathology and histology of the biopsy specimen revealed a pilomatrixoma comprising basaloid cells and ghost cells,with basaloid cells expressing high bcl-2 levels.Instead of apoptosis,the emergence and growth of pilomatrixoma-induced microinflammation around the hair follicles of the eyebrow may lead to eyebrow loss.展开更多
The aim of the present study was to explore the effects of annexin A1(ANXA1) mimetic peptide AC2-26 on sepsis-induced cardiomyocyte apoptosis in vivo and in vitro and the underlying mechanisms.In the in vivo study,a r...The aim of the present study was to explore the effects of annexin A1(ANXA1) mimetic peptide AC2-26 on sepsis-induced cardiomyocyte apoptosis in vivo and in vitro and the underlying mechanisms.In the in vivo study,a rat septic model was established by the cecal ligation and puncture (CLP).The rats were divided into control group,sepsis group and AC2-26 group.The rats in the AC2-26 group were intraperitoneally injected with AC2-26(1mg/kg)2h before CLP,and those in the control group and sepsis group were injected with the same volume of normal saline.The myocardial tissue was examined by hematoxylin and eosin (HE)staining and transmission electron microscopy (TEM).Furthermore,myocardial apoptosis was measured by terminal dUTP nick end-labeling (TUNEL)assay.In the in vitro study,H9C2cells were cultured and divided into three groups:control group,in which cells were only given the basic culture medium;LPS group,in which cells were treated with 10μg/mL LPS;AC2-26 group,in which cells were treated with 0.5μmol/L AC2-262h before 10μg/mL LPS was given.The apoptosis of H9C2 cells was detected by flow cytometry.The levels of lipoxin A4 receptor (LXA4),phosphoinositide3-kinase (PI3K)and protein kinase B (PKB or AKT)protein were measured by Western blotting, the activity of NF-KB and the level of TNF-α by ELISA and the activities of caspase-3/8by using the caspase activity kits.The in vivo study showed that the myocardial pathological damage and myocardial ultrastructural damage were significantly alleviated and the myocardial apoptosis significantly decreased in the AC2-26 group as compared with the sepsis group (P<0.05 for all). The in vivo study revealed that the apoptosis of H9C2 cells was profoundly ameliorated in the AC2-26 group relative to the sepsis group (P<0.05).The protein expression levels of LXA4 were significantly up-regulated,and those of PI3K and AKT prominently down-regulated in the AC2-26 group when compared with those in the LPS group (P<0.05 for all).The activity of NF-κB was greatly inhibited and the level of TNF-α markedly decreased in the AC2-26 group as compared with those in the LPS group (P<0.05 for all).AC2-26 treatment also significantly suppressed the activities of caspase-3/8 in H9C2 cells.In conclusion,these findings suggest that AC2-26 may alleviate the sepsis-induced cardiomyocyte apoptosis in vivo and in vivo through the LXA4/PI3K/ AKT signaling pathway.展开更多
To overcome the ever-growing organic pollutions in the water system,abundant efforts have been dedicated to fabricating efficient Fenton-like carbon catalysts.However,the rational design of carbon catalysts with high ...To overcome the ever-growing organic pollutions in the water system,abundant efforts have been dedicated to fabricating efficient Fenton-like carbon catalysts.However,the rational design of carbon catalysts with high intrinsic activity remains a long-term goal.Herein,we report a new N-molecule-assisted self-catalytic carbonization process in augmenting the intrinsic Fenton-like activity of metal-organic-framework-derived carbon hybrids.During carbonization,the N-molecules provide alkane/ammonia gases and the formed iron nanocrystals act as the in situ catalysts,which result in the elaborated formation of carbon nanotubes(in situ chemical vapor deposition from alkane/iron catalysts)and micro-/meso-porous structures(ammonia gas etching).The obtained catalysts exhibited with abundant Fe/Fe-Nx/pyridinic-N active species,micro-/meso-porous structures,and conductive carbon nanotubes.Consequently,the catalysts exhibit high efficiency toward the degradation of different organic pollutions,such as bisphenol A,methylene blue,and tetracycline.This study not only creates a new pathway for achieving highly active Fenton-like carbon catalysts but also takes a step toward the customized production of advanced carbon hybrids for diverse energy and environmental applications.展开更多
The temperature drop of plants from the optimal requirements can increase tolerance to severe chilling stress. Photosynthesis and osmoregulators were analyzed during chilling stress to explore the adaptation mechanism...The temperature drop of plants from the optimal requirements can increase tolerance to severe chilling stress. Photosynthesis and osmoregulators were analyzed during chilling stress to explore the adaptation mechanisms that underlie the induction of chilling tolerance in response to suboptimal temperature. The relationships of these processes to suboptimal temperature acclimation in watermelon were then determined.Suboptimal temperature-acclimated watermelon plants demonstrated tolerance during chilling stress, as indicated by the decreased electrolyte leakage and malondialdehyde accumulation compared with those non-acclimated watermelon plants. Chilling-induced photoinhibition and reduction in CO2 assimilation rate were alleviated after suboptimal temperature acclimation. The xanthophyll cycle level was enhanced by improving thermal dissipation ability and avoiding light damage. Consequently, the chilling tolerance of suboptimal temperature-acclimated watermelon plants was enhanced. The osmoregulation ability induced by suboptimal temperature acclimation protected watermelon plants against chilling injury because of the accumulation of small molecular substances, such as soluble sugar and proline. The protein levels of Rubisco activase(ClRCA) and the gene expression of the Benson–Calvin cycle simultaneously increased in suboptimal temperature-acclimated watermelon plants during chilling stress. Chilling tolerance in watermelon plants induced by suboptimal temperature acclimation is associated with enhanced photosynthetic adaptability and osmoregulation ability.展开更多
Nansi Lake and Hongze Lake are both water storage lakes along the East Route of the South-to-North Water Transfer project(ESNT).Frequent changes in hydrologic properties are responsible factors for controlling the zoo...Nansi Lake and Hongze Lake are both water storage lakes along the East Route of the South-to-North Water Transfer project(ESNT).Frequent changes in hydrologic properties are responsible factors for controlling the zooplankton community assemblages in both lakes,so we studied the possible influence of water transfer and environmental factors on zooplankton community structure and abundance.Zooplankton assemblages were investigated seasonally for one year in both lakes;a total of 133 and 122 zooplankton taxa were identified in Nansi Lake and Hongze Lake,respectively.The most dominant rotifer species were littoral,e.g.,Keratella tecta,Keratella valga and Lecane lunaris in Nansi Lake and Brachionus angularis,Brachionus forficula and Polyarthra vulgaris in Hongze Lake.Comparatively,Nansi Lake had a higher Shannon-Wiener diversity index value(5.13),while Hongze Lake had a higher species richness index(4.21).The average number of zooplankton across seasons in Nansi Lake(protozoa:774±63 ind./L,rotifers:4817±212 ind./L,cladocerans:896±14 ind./L,copepod:435±42 ind./L)was comparatively lower than Hongze Lake(protozoa:1238±63 ind./L,rotifers:6576±112 ind./L,cladocerans:1013±20 ind./L,copepod:534±25 ind./L).Canonical correspondence analysis identified differing environmental gradients that were most responsible for influencing zooplankton communities in the two lakes(Hongze Lake:NH4-N,total nitrogen,transparency and pH;Nansi:pH,temperature and total phosphorus).Frequent changes related to water transfer in lakes favoured the diversity of rotifers and protozoa communities.Zooplankton habitat preference,changes in community structure and opportunistic peaks and extinction of certain taxa were also observed in the study lakes.展开更多
Mesoporous LiFePO4/C composites containing 80 wt% of highly dispersed LiFePO4 nanoparticles(4-6 nm) were fabricated using bimodal mesoporous carbon(BMC) as continuous conductive networks. The unique pore structure of ...Mesoporous LiFePO4/C composites containing 80 wt% of highly dispersed LiFePO4 nanoparticles(4-6 nm) were fabricated using bimodal mesoporous carbon(BMC) as continuous conductive networks. The unique pore structure of BMC not only promises good particle connectivity for LiFePO4, but also acts as a rigid nano-confinement support that controls the particle size. Furthermore, the capacities were investigated respectively based on the weight of LiFePO4 and the whole composite. When calculated based on the weight of the whole composite, it is 120 mAh·g-1at 0.1 C of the high loading electrode and 42 mAh·g-1at 10 C of the low loading electrode. The electrochemical performance shows that high LiFePO4 loading benefits large tap density and contributes to the energy storage at low rates, while the electrode with low content of LiFePO4 displays superior high rate performance, which can mainly be due to the small particle size, good dispersion and high utilization of the active material, thus leading to a fast ion and electron diffusion.展开更多
Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wa...Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wang W S et al,2018).The indica subspecies occupies 90%of the rice cultivation area,as opposed to only 10%occupied by the japonica subspecies,which is mainly distributed in northern China,Japan and Korea(Xu and Chen,2016).China is the only country in which japonica and indica are of equal importance.Two-thirds of the rice grown in China is indica,which is cultivated mostly in low-altitude and low-latitude regions,and one-third is japonica,farmed prevalently in high-altitude and high-latitude regions(Wang Y H et al,2018).After the introduction of semi-dwarf gene sdl,the ideal plant architecture breeding and the application of the Fi hybrid,accompanied by the improvement of cultivation methods and field management。展开更多
This study presents a simple technique for strengthening the adhesive-bond strength between aluminium(Al)substrate and carbon fibre reinforced polymer(CFRP) utilising resin pre-coating(RPC) with carbon nanotubes(CNTs)...This study presents a simple technique for strengthening the adhesive-bond strength between aluminium(Al)substrate and carbon fibre reinforced polymer(CFRP) utilising resin pre-coating(RPC) with carbon nanotubes(CNTs). The CNT-containing RPC solution with 90 wt% acetone and 10 wt% resin(without hardener) was applied onto Al substrates, where micro-/nano-vertical channels had been created by chemical or mechanical surface treatments to accommodate CNTs. RPC was able to fill all micro-/nano-cavities over the Al substrate surface, then CNTs were pulled into those vertical micro-channels by the capillary action generated from acetone evaporation.Normal epoxy adhesive(resin + hardener) was applied after the CNT-containing RPC treatment. CNTs bridging across the interface between the adhesive joint and Al substrate and sealing of micro-/nano-cavities by RPC effectively enhanced the interfacial shear bond strength between the Al substrate and CFRP by 30–100%depending on the Al substrate surface profiles. Al substrates with two different chemical treatments were compared in this study for the effectiveness of CNT interfacial reinforcement. Results from a steel substrate after sandblasting were also included for comparison.展开更多
Previous reports have suggested that Ang-(1-7)may have a protective effect in endothelial cells against high glucose(HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling pathway.In this stu...Previous reports have suggested that Ang-(1-7)may have a protective effect in endothelial cells against high glucose(HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling pathway.In this study,we have examined whether NF-κB-IL-1βand Heme oxygenase-1(HO-1)pathways contribute to the protection of Ang-(1-7)against hyperglycemia-induced inflammation and oxidative stress in human umbilical vein endothelial cells(HUVECs).Our results indicate that time-varying exposures of HUVECs,from 1 h to 24 h,to high glucose concentrations result in an increased expression of phosphorylated(p)-p65 and HO-1 in a time-dependent manner.As an inhibitor of NF-κB,pyrrolidinedithiocarbamic acid(PDTC)suppressed IL-1βproduction induced by HG.Of note,HUVECs previously treated with Ang-(1-7)(2μM)for 30 min before being exposed to HG concentrations significantly ameliorated the HG-increased in p-p65 and IL-1βexpression;whereas obviously up-regulated the level of HO-1,along with inhibition of oxidative stress,inflammation,and the HG-induced cytotoxicity.Importantly,when HUVECs were previously treated either with PDTC or IL-1Ra for 30 min before being exposed to HG,it significantly prevented damages caused by high glucose concentrations mentioned above,while the treatment of HO-1 inhibitor Sn-protoporphyrin(SnPP)before exposure to both HG and Ang-(1-7)significantly blocked the protective effect exerted by Ang-(1-7)on endothelial cells against injuries induced by HG mentioned above.To conclude,the data of this study showed that activation and inhibition of the NF-κB-IL-1βpathway and HO-1 pathway may constitute an important defense mechanism against endothelial cell damage caused by HG concentrations.We additionally gave new evidence showing that exogenous Ang-(1-7)exerts a protective effect on HUVECs against the HG-induced cell injury via the inhibition and the activation of the NF-κB-IL-1βpathway and the HO-1 pathway,respectively.展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China (grant nos. LQ22H150005 and LQ21H150005)
文摘Pilomatrixomas are benign tumors derived from hair stromal cells;however,their exact etiology is unknown.A 31-year-old woman presented with a subcutaneous mass located precisely above the left eyebrow,with shedding of eyebrow hair around the mass,and the shedding area gradually expanded.Surgical removal of the mass failed to prevent eyebrow loss,and the entire left eyebrow shed 6 months postoperatively.Pathology and histology of the biopsy specimen revealed a pilomatrixoma comprising basaloid cells and ghost cells,with basaloid cells expressing high bcl-2 levels.Instead of apoptosis,the emergence and growth of pilomatrixoma-induced microinflammation around the hair follicles of the eyebrow may lead to eyebrow loss.
基金the Hubei Provincial Natural Science Foundation(No.2018CFC847)the Hubei Cancer Hospital Foundation(No.2015C11).
文摘The aim of the present study was to explore the effects of annexin A1(ANXA1) mimetic peptide AC2-26 on sepsis-induced cardiomyocyte apoptosis in vivo and in vitro and the underlying mechanisms.In the in vivo study,a rat septic model was established by the cecal ligation and puncture (CLP).The rats were divided into control group,sepsis group and AC2-26 group.The rats in the AC2-26 group were intraperitoneally injected with AC2-26(1mg/kg)2h before CLP,and those in the control group and sepsis group were injected with the same volume of normal saline.The myocardial tissue was examined by hematoxylin and eosin (HE)staining and transmission electron microscopy (TEM).Furthermore,myocardial apoptosis was measured by terminal dUTP nick end-labeling (TUNEL)assay.In the in vitro study,H9C2cells were cultured and divided into three groups:control group,in which cells were only given the basic culture medium;LPS group,in which cells were treated with 10μg/mL LPS;AC2-26 group,in which cells were treated with 0.5μmol/L AC2-262h before 10μg/mL LPS was given.The apoptosis of H9C2 cells was detected by flow cytometry.The levels of lipoxin A4 receptor (LXA4),phosphoinositide3-kinase (PI3K)and protein kinase B (PKB or AKT)protein were measured by Western blotting, the activity of NF-KB and the level of TNF-α by ELISA and the activities of caspase-3/8by using the caspase activity kits.The in vivo study showed that the myocardial pathological damage and myocardial ultrastructural damage were significantly alleviated and the myocardial apoptosis significantly decreased in the AC2-26 group as compared with the sepsis group (P<0.05 for all). The in vivo study revealed that the apoptosis of H9C2 cells was profoundly ameliorated in the AC2-26 group relative to the sepsis group (P<0.05).The protein expression levels of LXA4 were significantly up-regulated,and those of PI3K and AKT prominently down-regulated in the AC2-26 group when compared with those in the LPS group (P<0.05 for all).The activity of NF-κB was greatly inhibited and the level of TNF-α markedly decreased in the AC2-26 group as compared with those in the LPS group (P<0.05 for all).AC2-26 treatment also significantly suppressed the activities of caspase-3/8 in H9C2 cells.In conclusion,these findings suggest that AC2-26 may alleviate the sepsis-induced cardiomyocyte apoptosis in vivo and in vivo through the LXA4/PI3K/ AKT signaling pathway.
基金supported by the National Key R&D Program of China(2019YFA0110600 and 2019YFA0110601)National Natural Science Foundation of China(Nos.51603134,51903178,51803134,and 51703141)+1 种基金Sichuan Province’s Science and Technology Planning Project(No.2016GZ0350)the Postgraduate Course Construction Project of Sichuan University(No.2017KCSJ036)and for their financial support.
文摘To overcome the ever-growing organic pollutions in the water system,abundant efforts have been dedicated to fabricating efficient Fenton-like carbon catalysts.However,the rational design of carbon catalysts with high intrinsic activity remains a long-term goal.Herein,we report a new N-molecule-assisted self-catalytic carbonization process in augmenting the intrinsic Fenton-like activity of metal-organic-framework-derived carbon hybrids.During carbonization,the N-molecules provide alkane/ammonia gases and the formed iron nanocrystals act as the in situ catalysts,which result in the elaborated formation of carbon nanotubes(in situ chemical vapor deposition from alkane/iron catalysts)and micro-/meso-porous structures(ammonia gas etching).The obtained catalysts exhibited with abundant Fe/Fe-Nx/pyridinic-N active species,micro-/meso-porous structures,and conductive carbon nanotubes.Consequently,the catalysts exhibit high efficiency toward the degradation of different organic pollutions,such as bisphenol A,methylene blue,and tetracycline.This study not only creates a new pathway for achieving highly active Fenton-like carbon catalysts but also takes a step toward the customized production of advanced carbon hybrids for diverse energy and environmental applications.
基金funded by Hubei Provincial Natural Science Foundation of China (Grant No. 2015CFB385)the National Natural Science Foundation of China (Grant No. 31501783)the earmarked fund for Modern Agro-industry Technology Research System (Grant No. CARS-25)
文摘The temperature drop of plants from the optimal requirements can increase tolerance to severe chilling stress. Photosynthesis and osmoregulators were analyzed during chilling stress to explore the adaptation mechanisms that underlie the induction of chilling tolerance in response to suboptimal temperature. The relationships of these processes to suboptimal temperature acclimation in watermelon were then determined.Suboptimal temperature-acclimated watermelon plants demonstrated tolerance during chilling stress, as indicated by the decreased electrolyte leakage and malondialdehyde accumulation compared with those non-acclimated watermelon plants. Chilling-induced photoinhibition and reduction in CO2 assimilation rate were alleviated after suboptimal temperature acclimation. The xanthophyll cycle level was enhanced by improving thermal dissipation ability and avoiding light damage. Consequently, the chilling tolerance of suboptimal temperature-acclimated watermelon plants was enhanced. The osmoregulation ability induced by suboptimal temperature acclimation protected watermelon plants against chilling injury because of the accumulation of small molecular substances, such as soluble sugar and proline. The protein levels of Rubisco activase(ClRCA) and the gene expression of the Benson–Calvin cycle simultaneously increased in suboptimal temperature-acclimated watermelon plants during chilling stress. Chilling tolerance in watermelon plants induced by suboptimal temperature acclimation is associated with enhanced photosynthetic adaptability and osmoregulation ability.
基金Supported by the Service Project of Special Institute of Chinese Academy of Sciences(No.Y55Z06)the Key Project in Frontier Science of Chinese Academy of Sciences(No.QYZDB-SSW-SMC041)+1 种基金the National Science Foundation of Jiangsu Province,China(No.BK20141268)the National Natural Science Foundation of China(No.31400486a)。
文摘Nansi Lake and Hongze Lake are both water storage lakes along the East Route of the South-to-North Water Transfer project(ESNT).Frequent changes in hydrologic properties are responsible factors for controlling the zooplankton community assemblages in both lakes,so we studied the possible influence of water transfer and environmental factors on zooplankton community structure and abundance.Zooplankton assemblages were investigated seasonally for one year in both lakes;a total of 133 and 122 zooplankton taxa were identified in Nansi Lake and Hongze Lake,respectively.The most dominant rotifer species were littoral,e.g.,Keratella tecta,Keratella valga and Lecane lunaris in Nansi Lake and Brachionus angularis,Brachionus forficula and Polyarthra vulgaris in Hongze Lake.Comparatively,Nansi Lake had a higher Shannon-Wiener diversity index value(5.13),while Hongze Lake had a higher species richness index(4.21).The average number of zooplankton across seasons in Nansi Lake(protozoa:774±63 ind./L,rotifers:4817±212 ind./L,cladocerans:896±14 ind./L,copepod:435±42 ind./L)was comparatively lower than Hongze Lake(protozoa:1238±63 ind./L,rotifers:6576±112 ind./L,cladocerans:1013±20 ind./L,copepod:534±25 ind./L).Canonical correspondence analysis identified differing environmental gradients that were most responsible for influencing zooplankton communities in the two lakes(Hongze Lake:NH4-N,total nitrogen,transparency and pH;Nansi:pH,temperature and total phosphorus).Frequent changes related to water transfer in lakes favoured the diversity of rotifers and protozoa communities.Zooplankton habitat preference,changes in community structure and opportunistic peaks and extinction of certain taxa were also observed in the study lakes.
基金supported by the National Natural Science Foundation of China (NSFC 21103184)the Ph.D.Programs Foundation (20100041110017) of Ministry of Education of Chinathe Fundamental Research Funds for the Central Universities
文摘Mesoporous LiFePO4/C composites containing 80 wt% of highly dispersed LiFePO4 nanoparticles(4-6 nm) were fabricated using bimodal mesoporous carbon(BMC) as continuous conductive networks. The unique pore structure of BMC not only promises good particle connectivity for LiFePO4, but also acts as a rigid nano-confinement support that controls the particle size. Furthermore, the capacities were investigated respectively based on the weight of LiFePO4 and the whole composite. When calculated based on the weight of the whole composite, it is 120 mAh·g-1at 0.1 C of the high loading electrode and 42 mAh·g-1at 10 C of the low loading electrode. The electrochemical performance shows that high LiFePO4 loading benefits large tap density and contributes to the energy storage at low rates, while the electrode with low content of LiFePO4 displays superior high rate performance, which can mainly be due to the small particle size, good dispersion and high utilization of the active material, thus leading to a fast ion and electron diffusion.
基金The study was supported by Natural Science Foundation(Grant No.U1708231).The data were provided by the Chinese new rice variety trial(2004-2018).
文摘Rice,Oryza sativa,is a major global food staple,90%of which is cultivated in Asian countries.Its two major subspecies are japonica and indica,differing in geographical distribution and morphological characteristics(Wang W S et al,2018).The indica subspecies occupies 90%of the rice cultivation area,as opposed to only 10%occupied by the japonica subspecies,which is mainly distributed in northern China,Japan and Korea(Xu and Chen,2016).China is the only country in which japonica and indica are of equal importance.Two-thirds of the rice grown in China is indica,which is cultivated mostly in low-altitude and low-latitude regions,and one-third is japonica,farmed prevalently in high-altitude and high-latitude regions(Wang Y H et al,2018).After the introduction of semi-dwarf gene sdl,the ideal plant architecture breeding and the application of the Fi hybrid,accompanied by the improvement of cultivation methods and field management。
基金Chang’an University of China for a visiting professor grant (2018-2020) for research collaboration between Chang’an University and University of Western Australia。
文摘This study presents a simple technique for strengthening the adhesive-bond strength between aluminium(Al)substrate and carbon fibre reinforced polymer(CFRP) utilising resin pre-coating(RPC) with carbon nanotubes(CNTs). The CNT-containing RPC solution with 90 wt% acetone and 10 wt% resin(without hardener) was applied onto Al substrates, where micro-/nano-vertical channels had been created by chemical or mechanical surface treatments to accommodate CNTs. RPC was able to fill all micro-/nano-cavities over the Al substrate surface, then CNTs were pulled into those vertical micro-channels by the capillary action generated from acetone evaporation.Normal epoxy adhesive(resin + hardener) was applied after the CNT-containing RPC treatment. CNTs bridging across the interface between the adhesive joint and Al substrate and sealing of micro-/nano-cavities by RPC effectively enhanced the interfacial shear bond strength between the Al substrate and CFRP by 30–100%depending on the Al substrate surface profiles. Al substrates with two different chemical treatments were compared in this study for the effectiveness of CNT interfacial reinforcement. Results from a steel substrate after sandblasting were also included for comparison.
基金supported by the Technology Planning Project of Huangpu District(201544-01)Medical Scientific Research Foundation of Guangdong Province(A2015287)+2 种基金Science and Technology Planning Project of Guangdong Province(2017ZC0474)Natural Science Foundation of Guangdong Province(2015A030313690)General Project of Dongguan City(Nos.201950715024922 and 2018507150241344).
文摘Previous reports have suggested that Ang-(1-7)may have a protective effect in endothelial cells against high glucose(HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling pathway.In this study,we have examined whether NF-κB-IL-1βand Heme oxygenase-1(HO-1)pathways contribute to the protection of Ang-(1-7)against hyperglycemia-induced inflammation and oxidative stress in human umbilical vein endothelial cells(HUVECs).Our results indicate that time-varying exposures of HUVECs,from 1 h to 24 h,to high glucose concentrations result in an increased expression of phosphorylated(p)-p65 and HO-1 in a time-dependent manner.As an inhibitor of NF-κB,pyrrolidinedithiocarbamic acid(PDTC)suppressed IL-1βproduction induced by HG.Of note,HUVECs previously treated with Ang-(1-7)(2μM)for 30 min before being exposed to HG concentrations significantly ameliorated the HG-increased in p-p65 and IL-1βexpression;whereas obviously up-regulated the level of HO-1,along with inhibition of oxidative stress,inflammation,and the HG-induced cytotoxicity.Importantly,when HUVECs were previously treated either with PDTC or IL-1Ra for 30 min before being exposed to HG,it significantly prevented damages caused by high glucose concentrations mentioned above,while the treatment of HO-1 inhibitor Sn-protoporphyrin(SnPP)before exposure to both HG and Ang-(1-7)significantly blocked the protective effect exerted by Ang-(1-7)on endothelial cells against injuries induced by HG mentioned above.To conclude,the data of this study showed that activation and inhibition of the NF-κB-IL-1βpathway and HO-1 pathway may constitute an important defense mechanism against endothelial cell damage caused by HG concentrations.We additionally gave new evidence showing that exogenous Ang-(1-7)exerts a protective effect on HUVECs against the HG-induced cell injury via the inhibition and the activation of the NF-κB-IL-1βpathway and the HO-1 pathway,respectively.
