Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in vivo.Continuous glucose monitoring based on implantable electrodes could induce pain an...Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in vivo.Continuous glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose detection.Microneedle technology is less invasive,yet microneedle adhesion with skin tissue is limited.In this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in vivo.The microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle array.Due to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into skin.The MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation state.Finally,the MASA was integrated into a monitoring system using custom circuitry.This work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients.展开更多
Developing techniques to effectively and real-time monitor and regulate the interior environment of biological objects is significantly important for many biomedical engineering and scientific applications, including ...Developing techniques to effectively and real-time monitor and regulate the interior environment of biological objects is significantly important for many biomedical engineering and scientific applications, including drug delivery, electrophysiological recording and regulation of intracellular activities. Semi-implantable bioelectronics is currently a hot spot in biomedical engineering research area, because it not only meets the increasing technical demands for precise detection or regulation of biological activities, but also provides a desirable platform for externally incorporating complex functionalities and electronic integration. Although there is less definition and summary to distinguish it from the well-reviewed non-invasive bioelectronics and fully implantable bioelectronics, semi-implantable bioelectronics have emerged as highly unique technology to boost the development of biochips and smart wearable device. Here, we reviewed the recent progress in this field and raised the concept of “Semi-implantable bioelectronics”, summarizing the principle and strategies of semi-implantable device for cell applications and in vivo applications, discussing the typical methodologies to access to intracellular environment or in vivo environment, biosafety aspects and typical applications. This review is meaningful for understanding in-depth the design principles, materials fabrication techniques, device integration processes, cell/tissue penetration methodologies, biosafety aspects, and applications strategies that are essential to the development of future minimally invasive bioelectronics.展开更多
Cancer is one of the leading causes of human death,despite enormous efforts to explore cancer biology and develop anticancer therapies.The main challenges in cancer research are establishing an efficient tumor microen...Cancer is one of the leading causes of human death,despite enormous efforts to explore cancer biology and develop anticancer therapies.The main challenges in cancer research are establishing an efficient tumor microenvironment in vitro and exploring efficient means for screening anticancer drugs to reveal the nature of cancer and develop treatments.The tumor microenvironment possesses human-specific biophysical and biochemical factors that are difficult to recapitulate in conventional in vitro planar cell models and in vivo animal models.Therefore,model limitations have hindered the translation of basic research findings to clinical applications.In this review,we introduce the recent progress in tumor-on-a-chip devices for cancer biology research,medicine assessment,and biomedical applications in detail.The emerging tumor-on-a-chip platforms integrating 3D cell culture,microfluidic tech no logy,and tissue engineeri ng have successfully mimicked the pivotal structural and functional characteristics of the in vivo tumor microenvironment.The recent advances in tumor-on-a-chip platforms for cancer biology studies and biomedical applications are detailed and an a lyzed in this review.This review should be valuable for further understanding the mechanisms of the tumor evolution process,screening anticancer drugs,and developing cancer therapies,and it addresses the challenges and potential opportunities in predicting drug screening and cancer treatment.展开更多
基金This work was financially supported by the National Key R&D Program of China(Nos.2021YFF1200700 and 2021YFA0911100)the National Natural Science Foundation of China(Nos.32171399,32171456,and T2225010)+6 种基金the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515012261)the Science and Technology Program of Guangzhou,China(No.202103000076)the Fundamental Research Funds for the Central Universities,Sun Yat-Sen University(No.22dfx02),and Pazhou Lab,Guangzhou(No.PZL2021KF0003)FML would like to thank the National Natural Science Foundation of China(Nos.32171335 and 31900954)JL would like to thank the National Natural Science Foundation of China(No.62105380)the China Postdoctoral Science Foundation(No.2021M693686)QQOY would like to thank the China Postdoctoral Science Foundation(No.2022M713645).
文摘Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in vivo.Continuous glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose detection.Microneedle technology is less invasive,yet microneedle adhesion with skin tissue is limited.In this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in vivo.The microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle array.Due to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into skin.The MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation state.Finally,the MASA was integrated into a monitoring system using custom circuitry.This work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients.
基金financial support from the National Natural Science Foundation of China(Grant Nos.32171399)the National Key R&D Program of China(Grant Nos.2021YFF1200700,2021YFA0911100)+1 种基金the National Natural Science Foundation of China(Grant Nos.32171456,32171335,61901535,31900954,62104264)。
文摘Developing techniques to effectively and real-time monitor and regulate the interior environment of biological objects is significantly important for many biomedical engineering and scientific applications, including drug delivery, electrophysiological recording and regulation of intracellular activities. Semi-implantable bioelectronics is currently a hot spot in biomedical engineering research area, because it not only meets the increasing technical demands for precise detection or regulation of biological activities, but also provides a desirable platform for externally incorporating complex functionalities and electronic integration. Although there is less definition and summary to distinguish it from the well-reviewed non-invasive bioelectronics and fully implantable bioelectronics, semi-implantable bioelectronics have emerged as highly unique technology to boost the development of biochips and smart wearable device. Here, we reviewed the recent progress in this field and raised the concept of “Semi-implantable bioelectronics”, summarizing the principle and strategies of semi-implantable device for cell applications and in vivo applications, discussing the typical methodologies to access to intracellular environment or in vivo environment, biosafety aspects and typical applications. This review is meaningful for understanding in-depth the design principles, materials fabrication techniques, device integration processes, cell/tissue penetration methodologies, biosafety aspects, and applications strategies that are essential to the development of future minimally invasive bioelectronics.
基金The work is supported in part by the National Natural Science Foundation of China(Nos.8177060,31970870,61771498,882061148011)the Guangdong Basic and Applied Basic Research Foundation(Grant Nos.2O2OA1515110940,2O2OA1515010665)+3 种基金100 Talents Program of Sun Yat-sen University(Grant Nos.76120-18841213)Basic Scientific Research Special Foundation of Sun Yat-sen University(Grant Nos.76120-18821104,20lgpy47,20lgzdl4)the Open Project of the Chinese Academy of Sciences(Grant No.SKT2006)the Open Fund of State Key Laboratory of Biocontrol at Sun Yat-sen University(2019SKLBC-KF06).
文摘Cancer is one of the leading causes of human death,despite enormous efforts to explore cancer biology and develop anticancer therapies.The main challenges in cancer research are establishing an efficient tumor microenvironment in vitro and exploring efficient means for screening anticancer drugs to reveal the nature of cancer and develop treatments.The tumor microenvironment possesses human-specific biophysical and biochemical factors that are difficult to recapitulate in conventional in vitro planar cell models and in vivo animal models.Therefore,model limitations have hindered the translation of basic research findings to clinical applications.In this review,we introduce the recent progress in tumor-on-a-chip devices for cancer biology research,medicine assessment,and biomedical applications in detail.The emerging tumor-on-a-chip platforms integrating 3D cell culture,microfluidic tech no logy,and tissue engineeri ng have successfully mimicked the pivotal structural and functional characteristics of the in vivo tumor microenvironment.The recent advances in tumor-on-a-chip platforms for cancer biology studies and biomedical applications are detailed and an a lyzed in this review.This review should be valuable for further understanding the mechanisms of the tumor evolution process,screening anticancer drugs,and developing cancer therapies,and it addresses the challenges and potential opportunities in predicting drug screening and cancer treatment.
