· AIM: To evaluate effect of hypertension on retinal ganglion cell(RGC) apoptosis, intraocular pressure(IOP),and the activation of endothelin-1(ET-1) signaling pathway in central retinal artery(CRA) in rats.·...· AIM: To evaluate effect of hypertension on retinal ganglion cell(RGC) apoptosis, intraocular pressure(IOP),and the activation of endothelin-1(ET-1) signaling pathway in central retinal artery(CRA) in rats.·METHODS: The experimental study was performed on20 male Sprague Dawley rats that were divided into control group, and hypertension groups. The hypertension was induced by subcutaneous deoxycorticoacetate(DOCA)10 mg/kg twice a week and administered 0.9% Na Cl solution daily for 2, 6, and 10 wk. Blood pressure(BP) was measured using animal BP analyzer. IOP was measured by handheld tonometry. Retinal tissue preparations by paraffin blocks were made after enucleation. The expression of ET-1, e NOS, ET-1 receptor A(ETRA), ET-1receptor B(ETRB), and phosphorylated myosin light chain kinase(MLCK), and caldesmon(Ca D) in CRA and RGC apoptosis were evaluated through immunofluorescent staining method then observed using laser scanning confocal microscopy.· RESULTS: BP significantly increased in all of the hypertension groups compared to control(P =0.001).Peak IOP elevation(7.78±4.14 mm Hg) and RGC apoptosis(576.15±33.28 Au) occurred on 2wk of hypertension. ET-1expression(1238.6±55.1 Au) and e NOS expression(2814.2±70.7 Au) were found highest in 2wk of hypertension,although the ratio of ET-1/e NOS decreased since 2wk.ETRAreached peak expression in 10 wk of hypertension(1219.4 ±6.3 Au), while ETRBsignificantly increased only in 2 weeks group(1069.2 ±9.6 Au). The highest MLCK expression(1190.09±58.32 Au), Ca D(1670.28±18.36 Au)were also found in 2wk of hypertension.·CONCLUSION: Hypertension effects to activation of ET-1 signaling pathway significantly in CRA, elevation of IOP, and RGC apoptosis. The highest value was achieved at 2wk, which is the development phase of hypertension.展开更多
基金Foundation:Directorate General of Higher Education(DGHE),National Education Ministry Republic of Indonesia
文摘· AIM: To evaluate effect of hypertension on retinal ganglion cell(RGC) apoptosis, intraocular pressure(IOP),and the activation of endothelin-1(ET-1) signaling pathway in central retinal artery(CRA) in rats.·METHODS: The experimental study was performed on20 male Sprague Dawley rats that were divided into control group, and hypertension groups. The hypertension was induced by subcutaneous deoxycorticoacetate(DOCA)10 mg/kg twice a week and administered 0.9% Na Cl solution daily for 2, 6, and 10 wk. Blood pressure(BP) was measured using animal BP analyzer. IOP was measured by handheld tonometry. Retinal tissue preparations by paraffin blocks were made after enucleation. The expression of ET-1, e NOS, ET-1 receptor A(ETRA), ET-1receptor B(ETRB), and phosphorylated myosin light chain kinase(MLCK), and caldesmon(Ca D) in CRA and RGC apoptosis were evaluated through immunofluorescent staining method then observed using laser scanning confocal microscopy.· RESULTS: BP significantly increased in all of the hypertension groups compared to control(P =0.001).Peak IOP elevation(7.78±4.14 mm Hg) and RGC apoptosis(576.15±33.28 Au) occurred on 2wk of hypertension. ET-1expression(1238.6±55.1 Au) and e NOS expression(2814.2±70.7 Au) were found highest in 2wk of hypertension,although the ratio of ET-1/e NOS decreased since 2wk.ETRAreached peak expression in 10 wk of hypertension(1219.4 ±6.3 Au), while ETRBsignificantly increased only in 2 weeks group(1069.2 ±9.6 Au). The highest MLCK expression(1190.09±58.32 Au), Ca D(1670.28±18.36 Au)were also found in 2wk of hypertension.·CONCLUSION: Hypertension effects to activation of ET-1 signaling pathway significantly in CRA, elevation of IOP, and RGC apoptosis. The highest value was achieved at 2wk, which is the development phase of hypertension.
