BACKGROUND Gastrointestinal symptoms are common in patients with uremia undergoing hemodialysis,and these symptoms seriously affect patients'prognosis.AIM To assess the occurrence and factors influencing gastroint...BACKGROUND Gastrointestinal symptoms are common in patients with uremia undergoing hemodialysis,and these symptoms seriously affect patients'prognosis.AIM To assess the occurrence and factors influencing gastrointestinal symptoms in patients with uremia undergoing hemodialysis.METHODS We retrospectively selected 98 patients with uremia who underwent regular hemo-dialysis treatment in the blood purification center of our hospital from December 2022 to December 2023.The gastrointestinal symptoms and scores of each dimension were evaluated using the Gastrointestinal Symptom Grading Scale(GSRS).Patients were divided into gastrointestinal symptoms and no gastrointestinal symptom groups according to whether they had gastrointestinal symptoms.The factors that may affect gastrointestinal symptoms were identified by single-factor analysis.Multiple logistic regression analysis was performed to identify independent risk factors for gastrointestinal symptoms.RESULTS Gastrointestinal symptoms included indigestion,constipation,reflux,diarrhea,abdominal pain,and eating disorders,and the total average GSRS score was 1.35±0.47.This study showed that age,number of tablets,dialysis time,glucocorticoid,parathyroid hormone(PTH),combined diabetes mellitus and C-reactive protein(CRP)were independent risk factors for gastrointestinal symptoms in patients with uremia undergoing hemodialysis,whereas body mass index(BMI),hemoglobin(Hb),and urea clearance index were independent protective factors(P<0.05).CONCLUSION Gastrointestinal symptoms are mostly mild in patients with uremia undergoing hemodialysis,most commonly including dyspepsia,eating disorders,and gastroesophageal reflux.The independent influencing factors mainly include the BMI,age,number of pills taken,dialysis time,urea clearance index,Hb,use of glucocorticoids,and thyroid hormone level.PTH,CRP,and diabetes are clinically related factors influencing the occurrence of gastrointestinal symptoms,and targeted prevention can be performed.展开更多
Background:Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff.The attenuation function of biochanin A on blood-brain barrier(BBB)damage induced by cerebral ...Background:Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff.The attenuation function of biochanin A on blood-brain barrier(BBB)damage induced by cerebral ischemia-reperfusion remains unclear.Methods:C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion(MCAO)followed by 24 h reperfusion.The infarct volume of the brain was stained by TTC,while leakage of the brain was quantitatively stained by Evans blue,and the neurologic deficit score was measured.Microglial-induced morphologic changes were observed via immunofluorescence staining,and rolling and adhering leukocytes in venules were observed via two-photon imaging,while the inner fluorescein isothiocyanate-albumin of venules were compared with those of surrounding interstitial area through venular albumin leakage.Results:The attenuation effect of biochanin A on tight junction injury was compared in ischemia-reperfusion mice or conventional knockdown of leucine-richα2-glycoprotein 1(Lrg1)mice.Biochanin A could ameliorate BBB injury in mice with cerebral ischemiareperfusion in a dose-dependent manner by strengthening the immunostaining volume of occludin,claudin-5,and zonula occludens-1.The amoeba morphologic changes of microglial combined with the elevated expression of Lrg1 could be relieved under the treatment of biochanin A.Biochanin A played a countervailing role on the rolling leukocytes in the vessel,while the leakage of blood vessels was reduced.Biochanin A diminished its functions to further improved attenuation for tight junction injury on conventional Lrg1-knockout mice,as well as the inhibition effects on TGF-β1,and the phosphorylation of suppressor of mothers against decapentaplegic 2(Smad2)/Smad2 via western blot assay.Conclusion:Biochanin A could alleviate tight junction injury induced by cerebral ischemiareperfusion and blocked the Lrg1/TGF-β/Smad2 pathway to modulate leukocyte migration patterns.展开更多
The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chroma...The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography.The molecular mechanism of D.odorifera leaves on cerebral I/R was investigated.Methods:Serial affinity chromatography based on D.odorifera leaves extract(DLE)affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h.Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed proteins between control and DLE-affinity matrices.The hub genes were screened based on weighted gene co-expression network analysis(WGCNA).Then,predictive biology and potential experimental verification were performed for the candidate genes.The protective role of DLE in blood-brain barrier damage in cerebral I/R mice was evaluated by the leakage of Evans blue,western blotting,immunohistochemistry,and immunofluorescent staining.Results:952 differentially expressed proteins were classified into seven modules based on WGCNA under soft threshold 6.Based on WGCNA,AKT1,PIK3CA,NOS3,SMAD3,SMAD1,IL6,MAPK1,TGFBR2,TGFBR1,MAPK3,IGF1R,LRG1,mTOR,ROCK1,TGFB1,IL1B,SMAD2,and SMAD518 candidate hub proteins were involved in turquoise module.TGF-β,MAPK,focal adhesion,and adherens junction signaling pathway were associated with candidate hub proteins.Gene ontology analysis demonstrated that candidate hub proteins were related to the TGF-βreceptor signaling pathway,common-partner SMAD protein phosphorylation,etc.DLE could significantly reduce the leakage of Evans blue in mice with cerebral I/R,while attenuating the expression of occludin,claudin-5,and zonula occludens-1.Western blotting demonstrated that regulation of TGF-β/SMAD signaling pathway played an essential role in the protective effect of DLE.Conclusion:Thus,a number of candidate hub proteins were identified based on DLE affinity chromatography through WGCNA.DLE could attenuate the dysfunction of bloodbrain barrier in the TGF-β/SMAD signaling pathway induced by cerebral I/R.展开更多
DEAR EDITOR,Dramatic changes in habitat can have a range of effects on organisms(Bi & Zhang, 2021). Plotosus lineatus is a representative fish within Ostariophysi showing adaptation from freshwater habitats to the...DEAR EDITOR,Dramatic changes in habitat can have a range of effects on organisms(Bi & Zhang, 2021). Plotosus lineatus is a representative fish within Ostariophysi showing adaptation from freshwater habitats to the ocean. Here, we sequenced the genome of P. lineatus using long-read and Hi-C sequencing technology. Based on the high-quality chromosome-level genome, we explored the molecular basis of various P. lineatus features, including large genome with fewer chromosomes, novel dendritic organ(DO).展开更多
Caspase-1-mediated IL-1β production is generally controlled by two pathways. Toll-like receptors （TLRs） recognize pathogen-derived products and induce NF-KB-dependent pro-IL-1β transcription; NOD-like receptors （...Caspase-1-mediated IL-1β production is generally controlled by two pathways. Toll-like receptors （TLRs） recognize pathogen-derived products and induce NF-KB-dependent pro-IL-1β transcription; NOD-like receptors （NLRs） assemble caspase-l-activating inflammasome complexes that sense bacterial products/danger signals. Through a targeted chemical screen, we identify bromoxone, a marine natural product, as a specifc and potent inhibitor of the caspase-1 pathway. Bromoxone is effective over diverse inflammatory stimuli including TLR ligands plus ATP/nigeri- cin, cytosolic DNA, flagellin and Bacillus anthracis lethal toxin. Bromoxone also efficiently suppresses easpase-1 acti- vation triggered by several types of bacterial infection. Bromoxone acts upstream or at the level of the inflammasome in a transcription-independent manner. Bromoxone also inhibits pro-IL-1β expression by targeting components up- stream of IKK in the TLR-NF-kB pathway. The unique dual activities of bromoxone are shared by the known TAK1 inhibitor that specifically blocks Nalp3 inflammasome activation. Hinted from the mechanistic and pharmacological properties of bromoxone, we further discover that several known NF-KB inhibitors that act upstream of IKK, but not those targeting IKK or IKK downstream, are potent blockers of different NLRs-mediated caspase-1 activation. Our study uncovers a possible non-transcriptional molecular link between the NLR （Nalp3）-mediated inflammasome pathway and TLR-NF-kB signaling, and suggests a potential strategy to develop new anti-inflammatory drugs.展开更多
Adolescence is a critical period for neurodevelopment. Evidence from animal studies suggests that isolated rearing can exert negative effects on behavioral and brain development. The present study aimed to investigate...Adolescence is a critical period for neurodevelopment. Evidence from animal studies suggests that isolated rearing can exert negative effects on behavioral and brain development. The present study aimed to investigate the effects of adolescent social isolation on latent inhibition and brain-derived neurotrophic factor levels in the forebrain of adult rats. Male Wistar rats were randomly divided into adolescent isolation （isolated housing, 38-51 days of age） and social groups. Latent inhibition was tested at adulthood. Brain-derived neurotrophic factor levels were measured in the medial prefrontal cortex and nucleus accumbens by an enzyme-linked immunosorbent assay. Adolescent social isolation impaired latent inhibition and increased brain-derived neurotrophic factor levels in the medial prefrontal cortex of young adult rats. These data suggest that adolescent social isolation has a profound effect on cognitive function and neurotrophin levels in adult rats and may be used as an animal model of neurodevelopmental disorders.展开更多
The traditional ＂brain-derived neurotrophic factor （BDNF） hypothesis of depression＂ proposes that impairment of the BDNF signaling pathway in the hippocampus and prefrontal cortex participates in the pathophysiolo...The traditional ＂brain-derived neurotrophic factor （BDNF） hypothesis of depression＂ proposes that impairment of the BDNF signaling pathway in the hippocampus and prefrontal cortex participates in the pathophysiology of depression, and antidepressants act by recovering/enhancing BDNF signal transduction. Recent studies have suggested that BDNF signaling pathways exert more diverse and complex effects on depression onset and antidepressant therapy than originally thought, which include： （1） inhibition of the BDNF-TrkB signaling pathway in the hippocampus and/or prefrontal cortex does not induce the depression-like behavioral phenotype, but significantly diminishes therapeutic effects, which suggests that the BDNF-TrkB signaling pathway lacks direct or key effects on occurrence of emotional disorders, whereas an intact and normal BDNF-TrkB signaling pathway is necessary for antidepressant therapy. （2） The BDNF-TrkB signaling pathway exhibits opposite regulatory effects on depressive behavior in the hippocampus-prefrontal cortex network and mesolimbic system, which suggests that BDNF regulates emotion by affecting the emotion-related neural network, but not a single brain region. （3） The BDNF-TrkB and proBDNF-p75Nm signaling pathways in the brain, respectively, enhance and suppress hippocampal neural plasticity, which demonstrated that different BDNF signaling pathways interact and restrict each other in the regulation of neural plasticity and emotional behaviors. （4） BDNF gene polymorphism might be associated with susceptibility to depression. These new findings extend our understanding of neuronal pathways and mechanisms of action of BDNF signaling and contribute to improved views to traditional ＂neurotrophic factor hypothesis of depression＂.展开更多
Objective:To investigate the effect of miR-217 on sepsis induced lung injury in rats. Methods:75 SD healthy male rats (SPF grade) were randomly divided into normal group, sham operation group, model group, mir-217 MIC...Objective:To investigate the effect of miR-217 on sepsis induced lung injury in rats. Methods:75 SD healthy male rats (SPF grade) were randomly divided into normal group, sham operation group, model group, mir-217 MICs control group, mir-217 MICs control group and mir-217 MICs group. The rats in model group, mir-217 MICs control group and mir-217 MICs group were used to establish sepsis lung injury model by CLP Vein injection of 10 μ L mir-217 MICs control, 10 μ L mir-217 mics, normal group, sham operation group, model group, tail vein injection of normal saline volume. The pathological changes, wet / dry weight (w / D), arterial blood gas, TNF -α, IL-6, IL-1 β, MPO, PMN cell count, mir-217, TLR4 Signal pathway protein expression were evaluated.Results:The pathological scores, W/D, pH and BE of the mir-217 mimics group were all lower than that of the mir-217 mimics control group, while the PaO2 was higher than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The levels of TNF -α , IL-6, IL-1 and MPO in serum and alveolar lavage fluid of the mir-217 mimics group were lower than those of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The protein content and PMN cell count in the alveolar lavage fluid of the mir-217 mimics group were lower than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The expression level of mir-217 in the lung tissues of the mir-217 mimics group was higher than that of the mir-217 mimics control group, and the expression level of TLR4 signaling protein in the lung tissues of the mir-217 mimics control group was lower than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05).Conclusion:Mir-217 overexpression can inhibit TLR4 Signal transduction pathway, block inflammatory response, improve lung injury caused by sepsis and protect lung tissue.展开更多
Let X be an irreducible Hermitian symmetric space of compact type(IHSS for short).In this paper,we give the irreducible decomposition of SymrTX.As a by-product,we give a cohomological characterization of the rank of X...Let X be an irreducible Hermitian symmetric space of compact type(IHSS for short).In this paper,we give the irreducible decomposition of SymrTX.As a by-product,we give a cohomological characterization of the rank of X.Moreover,we introduce pseudoeffective thresholds to measure the bigness of tangent bundles of smooth complex projective varieties precisely and calculate them for irreducible Hermitian symmetric spaces of compact type.展开更多
Transition metal dichalcogenides(TMDs)have been regarded as promising cathodes for aqueous zinc-ion batteries(AZIBs)but suffer from sluggish reaction kinetics due to their poor conductivity and the strong electrostati...Transition metal dichalcogenides(TMDs)have been regarded as promising cathodes for aqueous zinc-ion batteries(AZIBs)but suffer from sluggish reaction kinetics due to their poor conductivity and the strong electrostatic interaction between Zn-ion and cathode materials.Herein,a well-defined structure with MoSSe nanosheets vertically anchored on graphene is used as the cathode for AZIBs.The dissolution of Se into MoS2 lattice together with heterointerface design via developing C-O-Mo bonds improves the inherent conductivity,enlarges interlayer spacing,and generates abundant anionic vacancies.As a result,the Zn2+intercalation/deintercalation process is greatly improved,which is confirmed by theoretical modeling and ex-situ experimental results.Remarkably,the assembled AZIBs exhibit high-rate capability(124.2 mAh·g^(−1)at 5 A·g^(−1))and long cycling life(83%capacity retention after 1,200 cycles at 2 A·g^(−1)).Moreover,the assembled quasi-solid-state Zn-ion batteries demonstrate a stable cycling performance over 100 cycles and high capacity retention over 94%after 2,500 bending cycles.This study provides a new strategy to unlock the electrochemical activity of TMDs via interface design and atomic engineering,which can also be applied to other TMDs for multivalent batteries.展开更多
Peracetic acid(CH_(3)C(O)OOH,PAA)-based heterogeneous advanced oxidation process(AOP)has attacked intensive interests due to production of various reactive species.Herein,Co(OH)_(2)nanoparticles decorated biochar(Co(O...Peracetic acid(CH_(3)C(O)OOH,PAA)-based heterogeneous advanced oxidation process(AOP)has attacked intensive interests due to production of various reactive species.Herein,Co(OH)_(2)nanoparticles decorated biochar(Co(OH)_(2)/BC)was fabricated by a simple and controllable method,which was used to degrade tetracycline hydrochloride(TTCH)in water through PAA activation.The results indicated that 100%TTCH(C_(0)=10μmol/L)degradation efficiency was realized within 7 min at pH 7,with a high kinetic rate constant(k_(1))of 0.64 min^(-1)by the optimized Co(OH)_(2)/BC.Material characterizations suggested that Co(OH)_(2)nanoparticle was successfully decorated on biochar,leading to more active sites and electronic structure alteration of biochar,thus greatly promoting the catalytic cleavage of PAA for radicals production.Then,the reactive oxygen species(ROS)quenching experiments and electron paramagnetic resonance(EPR)analysis demonstrated the key species were alkoxyl radicals(R–O^(·),mainly CH_(3)CO_(2)^(·)and CH_(3)CO_(3)^(·)),HO^(·)and^(1)O_(2)in this system.Besides,density functional theory(DFT)calculation on Fukui index further revealed that the vulnerable sites of TTCH and three possible degradation pathways were proposed.This study can provide a new strategy for synthesis functional materials in PAA activation AOPs for removal of antibiotics in water.展开更多
A new family of trinuclear windwheel complexes with molecular formula [M^(II)_(3)(tpa)_(3)(μ-ttc)](ClO_(4))_(3)·n(sol) (ttc = 1,3,5-triazine-2,4,6-trithiol;tpa = tris(2-pyridylmethyl)amine;M = Mn,n = 2...A new family of trinuclear windwheel complexes with molecular formula [M^(II)_(3)(tpa)_(3)(μ-ttc)](ClO_(4))_(3)·n(sol) (ttc = 1,3,5-triazine-2,4,6-trithiol;tpa = tris(2-pyridylmethyl)amine;M = Mn,n = 2,sol = CH_(3)CN,1;M = Co,n = 1,sol = CH_(3)CN,2;M = Ni,n = 0,3) were synthesized and characterized.Single-crystal X-ray diffraction revealed that three metal centers in 1—3 are connected by ttc bridge,forming a regular triangular MII3 core.Each metal center is bonded by chelating S,N atoms from ttc and by N atoms from tpa.Magnetic studies showed that 1—3 displayed antiferromagnetic behavior and further gave the easy-axis anisotropy (D = −0.77 cm^(−1) for 1 and −8.13 cm^(−1) for 2) and easy-plane anisotropy (D = 5.08 cm^(−1) for 3).Moreover,2 exhibited field-induced slow magnetic relaxation behavior and their effective energy barriers were roughly evaluated Ueff = 6.9 K.展开更多
Objective To study the flavonoids from the heartwood of Dalbergia cochinchinensis. Methods The chemical constituents were isolated and purified by combination of silica gel, macroporous resin, Sephadex LH-20, and ODS ...Objective To study the flavonoids from the heartwood of Dalbergia cochinchinensis. Methods The chemical constituents were isolated and purified by combination of silica gel, macroporous resin, Sephadex LH-20, and ODS column chromatography. Their structures were identified by means of spectral analysis. Results Fifteen flavonoids were isolated and identified as pinocembrin （1）, liquiritigenin （2）, galangin （3）, 7-hydroxy- 6-methoxyflavone （4）, naringenin （5）, alpinetin （6）, 2,3-dimethoxyxanthone （7）, 6,4＇-dihydroxy-7-methoxy-flavan （8）, mucronulatol （9）, 7,8-dihydroxyflavanone （10）, 5,7,3＇,5＇-tetrahydroxyflavanone （11 ）, 4,2＇,5＇-trihydroxy-4＇-methoxychalcone （12）, isoliquiritigenin （13）, butein （14）, and 3＇,5＇,5,7-tetrahydroxy-6-C-I^-D-glucopyranosyl- flavanone （15）, respectively. Conclusion Compounds 7, 8, ｜ 0, and 15 are isolated from the plants of Dalbergia L. f. for the first time, and compounds 1, 3, 5, 6, 9, 11, 12, and 14 are isolated from this plant for the first time.展开更多
The inflammasome is an emerging new pathway in innate immune defense against microbial infection or endogenous danger signals.The inflammasome stimulates activation of inflammatory caspases,mainly caspase-1.Caspase-1 ...The inflammasome is an emerging new pathway in innate immune defense against microbial infection or endogenous danger signals.The inflammasome stimulates activation of inflammatory caspases,mainly caspase-1.Caspase-1 activation is responsible for processing and secretion of IL-1βand IL-18 as well as for inducing macrophage pyroptotic death.Assembly of the large cytoplasmic inflammasome complex is thought to be mediated by members of NOD-like receptor(NLR)family.While functions of most of the NLR proteins remain to be defined,several NLR proteins including NLRC4 have been shown to assemble distinct inflammasome complexes.These inflammasome pathways,particularly the NLRC4 inflammasome,play a critical role in sensing and restricting diverse types of bacterial infections.Here we review recent advances in defining the exact bacterial ligands and the ligand-binding receptors involved in NLRC4 inflammasome activation.Implications of the discovery of the NAIP family of inflammasome receptors for bacterial flagellin and type III secretion apparatus on future inflammasome and bacterial infection studies are also discussed.展开更多
Silicon nanomembrane（SiNM）transistors gated by chitosan membrane were fabricated on plastic substrate to mimic synapse behaviors.The device has both a bottom proton gate（BG）and multiple side gates（SG）.Electrical...Silicon nanomembrane（SiNM）transistors gated by chitosan membrane were fabricated on plastic substrate to mimic synapse behaviors.The device has both a bottom proton gate（BG）and multiple side gates（SG）.Electrical transfer properties of BG show hysteresis curves different from those of typical SiO2 gate dielectric.Synaptic behaviors and functions by linear accumulation and release of protons have been mimicked on this device：excitatory post-synaptic current（EPSC）and paired pulse facilitation behavior of biological synapses were mimicked and the paired-pulse facilitation index could be effectively tuned by the spike interval applied on the BG.Synaptic behaviors and functions,including short-term memory and long-term memory,were also experimentally demonstrated in BG mode.Meanwhile,spiking logic operation and logic modulation were realized in SG mode.展开更多
We report a bottom-up synthesis of a new graphyne allotrope-graphtetrayne(GTTY),which features four acetylene linkages between the adjacent benzene on the surface of copper under low temperature.The as-prepared GTTY e...We report a bottom-up synthesis of a new graphyne allotrope-graphtetrayne(GTTY),which features four acetylene linkages between the adjacent benzene on the surface of copper under low temperature.The as-prepared GTTY exhibited nanosheet morphology with an average thickness of 4.5 nm.Structural analysis based on high-resolution transmission electron microscopy(HR-TEM)/selected area electron diffraction(SAED)characterizations revealed high crystallinity formed with a ninefold stacking structure.The GTTY displayed excellent semiconductor properties bearing a band gap of 1.3 eV and a conductivity of 0.285 S m^(−1)at room temperature.Moreover,by the space-charge-limited current(SCLC)method,the measured hole and electron mobilities of the GTTY films reached up to 1.47×10^(3) cm^(2) V^(−1)S^(−1)and 2.98×10^(3)cm^(2)V^(−1)S^(−1),respectively.展开更多
Recently,Myles et al.(1)published a multi-center randomized controlled trial(RCT)concluding that compared to liberal fluid regimen,restricted fluid regimen did not improve clinical outcomes in patients after abdominal...Recently,Myles et al.(1)published a multi-center randomized controlled trial(RCT)concluding that compared to liberal fluid regimen,restricted fluid regimen did not improve clinical outcomes in patients after abdominal surgery.Perioperative fluid management plays an important role in postoperative care.As fluid accumulation was associated with poor outcomes in surgical patients(2),restricted fluid protocol was investigated to alleviate this situation.However,the conclusions remain conflicting.In contrast to Myles et al.'s finding,the benefits of restricted fluid protocol have been reported in several RCTs(PMID:12044376,14578723,21948211).展开更多
文摘BACKGROUND Gastrointestinal symptoms are common in patients with uremia undergoing hemodialysis,and these symptoms seriously affect patients'prognosis.AIM To assess the occurrence and factors influencing gastrointestinal symptoms in patients with uremia undergoing hemodialysis.METHODS We retrospectively selected 98 patients with uremia who underwent regular hemo-dialysis treatment in the blood purification center of our hospital from December 2022 to December 2023.The gastrointestinal symptoms and scores of each dimension were evaluated using the Gastrointestinal Symptom Grading Scale(GSRS).Patients were divided into gastrointestinal symptoms and no gastrointestinal symptom groups according to whether they had gastrointestinal symptoms.The factors that may affect gastrointestinal symptoms were identified by single-factor analysis.Multiple logistic regression analysis was performed to identify independent risk factors for gastrointestinal symptoms.RESULTS Gastrointestinal symptoms included indigestion,constipation,reflux,diarrhea,abdominal pain,and eating disorders,and the total average GSRS score was 1.35±0.47.This study showed that age,number of tablets,dialysis time,glucocorticoid,parathyroid hormone(PTH),combined diabetes mellitus and C-reactive protein(CRP)were independent risk factors for gastrointestinal symptoms in patients with uremia undergoing hemodialysis,whereas body mass index(BMI),hemoglobin(Hb),and urea clearance index were independent protective factors(P<0.05).CONCLUSION Gastrointestinal symptoms are mostly mild in patients with uremia undergoing hemodialysis,most commonly including dyspepsia,eating disorders,and gastroesophageal reflux.The independent influencing factors mainly include the BMI,age,number of pills taken,dialysis time,urea clearance index,Hb,use of glucocorticoids,and thyroid hormone level.PTH,CRP,and diabetes are clinically related factors influencing the occurrence of gastrointestinal symptoms,and targeted prevention can be performed.
基金supported by a Foundation Project:National Natural Science Foundation of China(Nos.82100417,81760094),ChinaThe Foundation of Jiangxi Provincial Department of Science and Technology Project(Nos.20202ACBL206001,20212BAB206022,20181BAB205026).
文摘Background:Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff.The attenuation function of biochanin A on blood-brain barrier(BBB)damage induced by cerebral ischemia-reperfusion remains unclear.Methods:C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion(MCAO)followed by 24 h reperfusion.The infarct volume of the brain was stained by TTC,while leakage of the brain was quantitatively stained by Evans blue,and the neurologic deficit score was measured.Microglial-induced morphologic changes were observed via immunofluorescence staining,and rolling and adhering leukocytes in venules were observed via two-photon imaging,while the inner fluorescein isothiocyanate-albumin of venules were compared with those of surrounding interstitial area through venular albumin leakage.Results:The attenuation effect of biochanin A on tight junction injury was compared in ischemia-reperfusion mice or conventional knockdown of leucine-richα2-glycoprotein 1(Lrg1)mice.Biochanin A could ameliorate BBB injury in mice with cerebral ischemiareperfusion in a dose-dependent manner by strengthening the immunostaining volume of occludin,claudin-5,and zonula occludens-1.The amoeba morphologic changes of microglial combined with the elevated expression of Lrg1 could be relieved under the treatment of biochanin A.Biochanin A played a countervailing role on the rolling leukocytes in the vessel,while the leakage of blood vessels was reduced.Biochanin A diminished its functions to further improved attenuation for tight junction injury on conventional Lrg1-knockout mice,as well as the inhibition effects on TGF-β1,and the phosphorylation of suppressor of mothers against decapentaplegic 2(Smad2)/Smad2 via western blot assay.Conclusion:Biochanin A could alleviate tight junction injury induced by cerebral ischemiareperfusion and blocked the Lrg1/TGF-β/Smad2 pathway to modulate leukocyte migration patterns.
基金supported by National Natural Science Foundation of China(Nos.82100417,81760094,81760724)The Foundation of Jiangxi Provincial Department of Science and Technology Project(Nos.20202ACBL206001,20212BAB206022,20181BAB205026)+1 种基金Youth Project of Jiangxi Education Department(No.GJJ200217)Open Project of Key Laboratory of Modern of TCM,Ministry of Education Jiangxi University of Traditional Chinese Medicine(TCM-2019010).
文摘The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography.The molecular mechanism of D.odorifera leaves on cerebral I/R was investigated.Methods:Serial affinity chromatography based on D.odorifera leaves extract(DLE)affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h.Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed proteins between control and DLE-affinity matrices.The hub genes were screened based on weighted gene co-expression network analysis(WGCNA).Then,predictive biology and potential experimental verification were performed for the candidate genes.The protective role of DLE in blood-brain barrier damage in cerebral I/R mice was evaluated by the leakage of Evans blue,western blotting,immunohistochemistry,and immunofluorescent staining.Results:952 differentially expressed proteins were classified into seven modules based on WGCNA under soft threshold 6.Based on WGCNA,AKT1,PIK3CA,NOS3,SMAD3,SMAD1,IL6,MAPK1,TGFBR2,TGFBR1,MAPK3,IGF1R,LRG1,mTOR,ROCK1,TGFB1,IL1B,SMAD2,and SMAD518 candidate hub proteins were involved in turquoise module.TGF-β,MAPK,focal adhesion,and adherens junction signaling pathway were associated with candidate hub proteins.Gene ontology analysis demonstrated that candidate hub proteins were related to the TGF-βreceptor signaling pathway,common-partner SMAD protein phosphorylation,etc.DLE could significantly reduce the leakage of Evans blue in mice with cerebral I/R,while attenuating the expression of occludin,claudin-5,and zonula occludens-1.Western blotting demonstrated that regulation of TGF-β/SMAD signaling pathway played an essential role in the protective effect of DLE.Conclusion:Thus,a number of candidate hub proteins were identified based on DLE affinity chromatography through WGCNA.DLE could attenuate the dysfunction of bloodbrain barrier in the TGF-β/SMAD signaling pathway induced by cerebral I/R.
基金supported by the National Natural Science Foundation of China (31872204)Fundamental Research Funds for the Central Universities (SWU120049)。
文摘DEAR EDITOR,Dramatic changes in habitat can have a range of effects on organisms(Bi & Zhang, 2021). Plotosus lineatus is a representative fish within Ostariophysi showing adaptation from freshwater habitats to the ocean. Here, we sequenced the genome of P. lineatus using long-read and Hi-C sequencing technology. Based on the high-quality chromosome-level genome, we explored the molecular basis of various P. lineatus features, including large genome with fewer chromosomes, novel dendritic organ(DO).
文摘Caspase-1-mediated IL-1β production is generally controlled by two pathways. Toll-like receptors （TLRs） recognize pathogen-derived products and induce NF-KB-dependent pro-IL-1β transcription; NOD-like receptors （NLRs） assemble caspase-l-activating inflammasome complexes that sense bacterial products/danger signals. Through a targeted chemical screen, we identify bromoxone, a marine natural product, as a specifc and potent inhibitor of the caspase-1 pathway. Bromoxone is effective over diverse inflammatory stimuli including TLR ligands plus ATP/nigeri- cin, cytosolic DNA, flagellin and Bacillus anthracis lethal toxin. Bromoxone also efficiently suppresses easpase-1 acti- vation triggered by several types of bacterial infection. Bromoxone acts upstream or at the level of the inflammasome in a transcription-independent manner. Bromoxone also inhibits pro-IL-1β expression by targeting components up- stream of IKK in the TLR-NF-kB pathway. The unique dual activities of bromoxone are shared by the known TAK1 inhibitor that specifically blocks Nalp3 inflammasome activation. Hinted from the mechanistic and pharmacological properties of bromoxone, we further discover that several known NF-KB inhibitors that act upstream of IKK, but not those targeting IKK or IKK downstream, are potent blockers of different NLRs-mediated caspase-1 activation. Our study uncovers a possible non-transcriptional molecular link between the NLR （Nalp3）-mediated inflammasome pathway and TLR-NF-kB signaling, and suggests a potential strategy to develop new anti-inflammatory drugs.
基金supported by the National Natural Science Foundation of China, grant No. 31070910, 91132728the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-J-8)the Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
文摘Adolescence is a critical period for neurodevelopment. Evidence from animal studies suggests that isolated rearing can exert negative effects on behavioral and brain development. The present study aimed to investigate the effects of adolescent social isolation on latent inhibition and brain-derived neurotrophic factor levels in the forebrain of adult rats. Male Wistar rats were randomly divided into adolescent isolation （isolated housing, 38-51 days of age） and social groups. Latent inhibition was tested at adulthood. Brain-derived neurotrophic factor levels were measured in the medial prefrontal cortex and nucleus accumbens by an enzyme-linked immunosorbent assay. Adolescent social isolation impaired latent inhibition and increased brain-derived neurotrophic factor levels in the medial prefrontal cortex of young adult rats. These data suggest that adolescent social isolation has a profound effect on cognitive function and neurotrophin levels in adult rats and may be used as an animal model of neurodevelopmental disorders.
基金the National Natural Science Foundation of China,No. 30500158,30670708the Major Direc-tion Program of Chinese Academy of Sciences,No. KSCX2-YW-R-131+1 种基金the National Basic Research Project (973 Program) of China,No. 2007CB512306the Development Foundation of Institute of Psychology,Chinese Academy of Sci-ences,No. O9KF013001
文摘The traditional ＂brain-derived neurotrophic factor （BDNF） hypothesis of depression＂ proposes that impairment of the BDNF signaling pathway in the hippocampus and prefrontal cortex participates in the pathophysiology of depression, and antidepressants act by recovering/enhancing BDNF signal transduction. Recent studies have suggested that BDNF signaling pathways exert more diverse and complex effects on depression onset and antidepressant therapy than originally thought, which include： （1） inhibition of the BDNF-TrkB signaling pathway in the hippocampus and/or prefrontal cortex does not induce the depression-like behavioral phenotype, but significantly diminishes therapeutic effects, which suggests that the BDNF-TrkB signaling pathway lacks direct or key effects on occurrence of emotional disorders, whereas an intact and normal BDNF-TrkB signaling pathway is necessary for antidepressant therapy. （2） The BDNF-TrkB signaling pathway exhibits opposite regulatory effects on depressive behavior in the hippocampus-prefrontal cortex network and mesolimbic system, which suggests that BDNF regulates emotion by affecting the emotion-related neural network, but not a single brain region. （3） The BDNF-TrkB and proBDNF-p75Nm signaling pathways in the brain, respectively, enhance and suppress hippocampal neural plasticity, which demonstrated that different BDNF signaling pathways interact and restrict each other in the regulation of neural plasticity and emotional behaviors. （4） BDNF gene polymorphism might be associated with susceptibility to depression. These new findings extend our understanding of neuronal pathways and mechanisms of action of BDNF signaling and contribute to improved views to traditional ＂neurotrophic factor hypothesis of depression＂.
文摘Objective:To investigate the effect of miR-217 on sepsis induced lung injury in rats. Methods:75 SD healthy male rats (SPF grade) were randomly divided into normal group, sham operation group, model group, mir-217 MICs control group, mir-217 MICs control group and mir-217 MICs group. The rats in model group, mir-217 MICs control group and mir-217 MICs group were used to establish sepsis lung injury model by CLP Vein injection of 10 μ L mir-217 MICs control, 10 μ L mir-217 mics, normal group, sham operation group, model group, tail vein injection of normal saline volume. The pathological changes, wet / dry weight (w / D), arterial blood gas, TNF -α, IL-6, IL-1 β, MPO, PMN cell count, mir-217, TLR4 Signal pathway protein expression were evaluated.Results:The pathological scores, W/D, pH and BE of the mir-217 mimics group were all lower than that of the mir-217 mimics control group, while the PaO2 was higher than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The levels of TNF -α , IL-6, IL-1 and MPO in serum and alveolar lavage fluid of the mir-217 mimics group were lower than those of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The protein content and PMN cell count in the alveolar lavage fluid of the mir-217 mimics group were lower than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05). The expression level of mir-217 in the lung tissues of the mir-217 mimics group was higher than that of the mir-217 mimics control group, and the expression level of TLR4 signaling protein in the lung tissues of the mir-217 mimics control group was lower than that of the mir-217 mimics control group, with statistically significant differences (P < 0.05).Conclusion:Mir-217 overexpression can inhibit TLR4 Signal transduction pathway, block inflammatory response, improve lung injury caused by sepsis and protect lung tissue.
文摘Let X be an irreducible Hermitian symmetric space of compact type(IHSS for short).In this paper,we give the irreducible decomposition of SymrTX.As a by-product,we give a cohomological characterization of the rank of X.Moreover,we introduce pseudoeffective thresholds to measure the bigness of tangent bundles of smooth complex projective varieties precisely and calculate them for irreducible Hermitian symmetric spaces of compact type.
基金supported by the National Natural Science Foundation of China(No.52172217)Natural Science Foundation of Guangdong Province(No.2021A1515010144)+4 种基金Natural Science Foundation of Shanghai(No.17ZR1414100)the Shenzhen Science and Technology Program(No.JCYJ20210324120400002)G.M.Z.appreciates the support from the National Key Research and Development Program of China(No.2019YFA0705700)Joint Funds of the National Natural Science Foundation of China(No.U21A20174)the Overseas Research Cooperation Fund of Tsinghua Shenzhen International Graduate School.
文摘Transition metal dichalcogenides(TMDs)have been regarded as promising cathodes for aqueous zinc-ion batteries(AZIBs)but suffer from sluggish reaction kinetics due to their poor conductivity and the strong electrostatic interaction between Zn-ion and cathode materials.Herein,a well-defined structure with MoSSe nanosheets vertically anchored on graphene is used as the cathode for AZIBs.The dissolution of Se into MoS2 lattice together with heterointerface design via developing C-O-Mo bonds improves the inherent conductivity,enlarges interlayer spacing,and generates abundant anionic vacancies.As a result,the Zn2+intercalation/deintercalation process is greatly improved,which is confirmed by theoretical modeling and ex-situ experimental results.Remarkably,the assembled AZIBs exhibit high-rate capability(124.2 mAh·g^(−1)at 5 A·g^(−1))and long cycling life(83%capacity retention after 1,200 cycles at 2 A·g^(−1)).Moreover,the assembled quasi-solid-state Zn-ion batteries demonstrate a stable cycling performance over 100 cycles and high capacity retention over 94%after 2,500 bending cycles.This study provides a new strategy to unlock the electrochemical activity of TMDs via interface design and atomic engineering,which can also be applied to other TMDs for multivalent batteries.
基金supported by the National Key Research and Development Program of China(Nos.2022YFF1303102 and 2021YFA1202500)Beijing Natural Science Foundation(No.8232035)+2 种基金the National Natural Science Foundation of China(Nos.52270053,52200083 and 52200084)the Beijing Nova Program(No.20220484215)the Key Special Projects for Science and Technology of Inner Mongolia(No.2021EEDSCXSFQZD001)。
文摘Peracetic acid(CH_(3)C(O)OOH,PAA)-based heterogeneous advanced oxidation process(AOP)has attacked intensive interests due to production of various reactive species.Herein,Co(OH)_(2)nanoparticles decorated biochar(Co(OH)_(2)/BC)was fabricated by a simple and controllable method,which was used to degrade tetracycline hydrochloride(TTCH)in water through PAA activation.The results indicated that 100%TTCH(C_(0)=10μmol/L)degradation efficiency was realized within 7 min at pH 7,with a high kinetic rate constant(k_(1))of 0.64 min^(-1)by the optimized Co(OH)_(2)/BC.Material characterizations suggested that Co(OH)_(2)nanoparticle was successfully decorated on biochar,leading to more active sites and electronic structure alteration of biochar,thus greatly promoting the catalytic cleavage of PAA for radicals production.Then,the reactive oxygen species(ROS)quenching experiments and electron paramagnetic resonance(EPR)analysis demonstrated the key species were alkoxyl radicals(R–O^(·),mainly CH_(3)CO_(2)^(·)and CH_(3)CO_(3)^(·)),HO^(·)and^(1)O_(2)in this system.Besides,density functional theory(DFT)calculation on Fukui index further revealed that the vulnerable sites of TTCH and three possible degradation pathways were proposed.This study can provide a new strategy for synthesis functional materials in PAA activation AOPs for removal of antibiotics in water.
基金supported by the Natural Science Foundation of Shandong Province(No.ZR2021QB140)the Fundamental Research Funds for the Central Universities(No.202113051)+2 种基金F.S.thanks the Youth Talent Project support(Nos.202001013196,202101013108)from Ocean University of Chinathe Open Project support(No.202017)from the State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen University and the Open Project support(No.M202317)from the State Key Laboratory of Microbial Technology,Shandong University.
文摘A new family of trinuclear windwheel complexes with molecular formula [M^(II)_(3)(tpa)_(3)(μ-ttc)](ClO_(4))_(3)·n(sol) (ttc = 1,3,5-triazine-2,4,6-trithiol;tpa = tris(2-pyridylmethyl)amine;M = Mn,n = 2,sol = CH_(3)CN,1;M = Co,n = 1,sol = CH_(3)CN,2;M = Ni,n = 0,3) were synthesized and characterized.Single-crystal X-ray diffraction revealed that three metal centers in 1—3 are connected by ttc bridge,forming a regular triangular MII3 core.Each metal center is bonded by chelating S,N atoms from ttc and by N atoms from tpa.Magnetic studies showed that 1—3 displayed antiferromagnetic behavior and further gave the easy-axis anisotropy (D = −0.77 cm^(−1) for 1 and −8.13 cm^(−1) for 2) and easy-plane anisotropy (D = 5.08 cm^(−1) for 3).Moreover,2 exhibited field-induced slow magnetic relaxation behavior and their effective energy barriers were roughly evaluated Ueff = 6.9 K.
基金National Natural Science Foundation of China(81360629)
文摘Objective To study the flavonoids from the heartwood of Dalbergia cochinchinensis. Methods The chemical constituents were isolated and purified by combination of silica gel, macroporous resin, Sephadex LH-20, and ODS column chromatography. Their structures were identified by means of spectral analysis. Results Fifteen flavonoids were isolated and identified as pinocembrin （1）, liquiritigenin （2）, galangin （3）, 7-hydroxy- 6-methoxyflavone （4）, naringenin （5）, alpinetin （6）, 2,3-dimethoxyxanthone （7）, 6,4＇-dihydroxy-7-methoxy-flavan （8）, mucronulatol （9）, 7,8-dihydroxyflavanone （10）, 5,7,3＇,5＇-tetrahydroxyflavanone （11 ）, 4,2＇,5＇-trihydroxy-4＇-methoxychalcone （12）, isoliquiritigenin （13）, butein （14）, and 3＇,5＇,5,7-tetrahydroxy-6-C-I^-D-glucopyranosyl- flavanone （15）, respectively. Conclusion Compounds 7, 8, ｜ 0, and 15 are isolated from the plants of Dalbergia L. f. for the first time, and compounds 1, 3, 5, 6, 9, 11, 12, and 14 are isolated from this plant for the first time.
基金by the National Basic Research Program of China(973 Program)(Grant Nos.2010CB835400 and 2012CB518700)Howard Hughes Medical Institute,USA.
文摘The inflammasome is an emerging new pathway in innate immune defense against microbial infection or endogenous danger signals.The inflammasome stimulates activation of inflammatory caspases,mainly caspase-1.Caspase-1 activation is responsible for processing and secretion of IL-1βand IL-18 as well as for inducing macrophage pyroptotic death.Assembly of the large cytoplasmic inflammasome complex is thought to be mediated by members of NOD-like receptor(NLR)family.While functions of most of the NLR proteins remain to be defined,several NLR proteins including NLRC4 have been shown to assemble distinct inflammasome complexes.These inflammasome pathways,particularly the NLRC4 inflammasome,play a critical role in sensing and restricting diverse types of bacterial infections.Here we review recent advances in defining the exact bacterial ligands and the ligand-binding receptors involved in NLRC4 inflammasome activation.Implications of the discovery of the NAIP family of inflammasome receptors for bacterial flagellin and type III secretion apparatus on future inflammasome and bacterial infection studies are also discussed.
基金Project supported by the National Natural Science Foundation of China(No.51322201)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20120071110025)Science and Technology Commission of Shanghai Municipality(No.14JC1400200)
文摘Silicon nanomembrane（SiNM）transistors gated by chitosan membrane were fabricated on plastic substrate to mimic synapse behaviors.The device has both a bottom proton gate（BG）and multiple side gates（SG）.Electrical transfer properties of BG show hysteresis curves different from those of typical SiO2 gate dielectric.Synaptic behaviors and functions by linear accumulation and release of protons have been mimicked on this device：excitatory post-synaptic current（EPSC）and paired pulse facilitation behavior of biological synapses were mimicked and the paired-pulse facilitation index could be effectively tuned by the spike interval applied on the BG.Synaptic behaviors and functions,including short-term memory and long-term memory,were also experimentally demonstrated in BG mode.Meanwhile,spiking logic operation and logic modulation were realized in SG mode.
基金This study was supported by the National Natural Science Foundation of China(nos.51722302,21674054,and 51972185).
文摘We report a bottom-up synthesis of a new graphyne allotrope-graphtetrayne(GTTY),which features four acetylene linkages between the adjacent benzene on the surface of copper under low temperature.The as-prepared GTTY exhibited nanosheet morphology with an average thickness of 4.5 nm.Structural analysis based on high-resolution transmission electron microscopy(HR-TEM)/selected area electron diffraction(SAED)characterizations revealed high crystallinity formed with a ninefold stacking structure.The GTTY displayed excellent semiconductor properties bearing a band gap of 1.3 eV and a conductivity of 0.285 S m^(−1)at room temperature.Moreover,by the space-charge-limited current(SCLC)method,the measured hole and electron mobilities of the GTTY films reached up to 1.47×10^(3) cm^(2) V^(−1)S^(−1)and 2.98×10^(3)cm^(2)V^(−1)S^(−1),respectively.
文摘Recently,Myles et al.(1)published a multi-center randomized controlled trial(RCT)concluding that compared to liberal fluid regimen,restricted fluid regimen did not improve clinical outcomes in patients after abdominal surgery.Perioperative fluid management plays an important role in postoperative care.As fluid accumulation was associated with poor outcomes in surgical patients(2),restricted fluid protocol was investigated to alleviate this situation.However,the conclusions remain conflicting.In contrast to Myles et al.'s finding,the benefits of restricted fluid protocol have been reported in several RCTs(PMID:12044376,14578723,21948211).
