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GA_3和kinetin在低温下对玉米和大豆种子萌发及幼苗发育影响的研究(英文) 被引量:12
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作者 王庆祥 吕桂兰 +1 位作者 fengzhang DonaldL.Smith 《作物学报》 CAS CSCD 北大核心 1999年第3期363-372,共10页
本文在控制温度条件下,研究了GA_3和kinetin在低温条件下对玉米和大豆种子萌发及幼苗发育的影响.结果表明,在萌发适宜温度25℃条件下两种药剂处理的效果均不显著,但在15℃和10℃较低温度条件下两种药剂对玉米和大豆种子萌发有明显促进作... 本文在控制温度条件下,研究了GA_3和kinetin在低温条件下对玉米和大豆种子萌发及幼苗发育的影响.结果表明,在萌发适宜温度25℃条件下两种药剂处理的效果均不显著,但在15℃和10℃较低温度条件下两种药剂对玉米和大豆种子萌发有明显促进作用,而且在10℃温度下药剂处理的效果好于在15℃温度下.在10℃低温条件下,对玉米和大豆种子萌发及幼苗发育最有效的处理是0.01mM GA_3和0.005 mM kinetin. 展开更多
关键词 大豆 低温 植物生长调节剂 玉米 种子萌发 GA3
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Heme oxygenase-1 alleviates ischemia/reperfusion injury in aged liver 被引量:6
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作者 Xue-HaoWang KeWang +6 位作者 fengzhang Xiang-ChengLi JunLi Wei-De JunGuo Xiao-FengQian YeFan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第5期690-694,共5页
AIM: To investigate if ischemia/reperfusion (I/R) injury in aged liver could be alleviated by heme oxygenase-1 (HO-1).METHODS: Three groups of SD rats (16 mo old) were studied. Group 1: control donors received physiol... AIM: To investigate if ischemia/reperfusion (I/R) injury in aged liver could be alleviated by heme oxygenase-1 (HO-1).METHODS: Three groups of SD rats (16 mo old) were studied. Group 1: control donors received physiological saline 24 h before their livers were harvested; group 2: donors were pretreated with hemih 24 h before their livers were harvested; and group 3: donors received hemin 24 h before their livers were harvested and zinc protoporphyrin (ZnPP,HO-1 inhibitor) was given to recipients at reperfusion. The harvested livers were stored in University of Wisconsin solution (4 ℃) for 6 h, and then transplanted to syngeneic rats. Serum glutamic oxaloacetic transaminase (SGOT),apoptotic cells, and apoptotic gene were measured 3, 6,12, 24, 48 h after reperfusion. We measured the apoptotic index by TUNEL, determined the expression of antiapoptotic Bcl-2 and proapoptotic (caspase-3) gene products by Western blot.RESULTS: After 3, 6, 12, 24, and 48 h of reperfusion, the SGOT levels (584.4±85.8 u/L, 999.2±125.2 u/L, 423.4±161.3u/L, 257.8±95.8 u/L, and 122.4±26.4 u/L) in hemin group were significantly (all P<0.05) lower than those in saline group (1082.2±101.2 u/L, 1775.2±328.3 u/L, 840.4±137.8 u/L,448.6±74.3 u/L, and 306.2±49.3 u/L). Liver HO-1 enzymatic activity correlated with beneficial effects of hemin and deleterious effects of adjunctive ZnPP treatment. Markedly less apoptotic (TUNEL+) liver cells 3, 6, 12, 24, and 48 h after reperfusion (5.16±0.73, 10.2±0.67, 9.28±0.78, 7.14±1.12,and 4.78±0.65) (P<0.05) could be detected in hemin liver grafts, as compared to controls (7.82±1.05, 15.94±1.82,11.67±1.59, 8.28±1.09, and 6.36±0.67). We detected the increased levels of Bcl-2 (1.5-fold) expression and compared with saline controls. These differences were most pronounced at 12 h after transplantation. In contrast, an active form of proapoptotic caspase-3 (p20) protein was found to be 2.9-fold lower at 24 h in hemin-pretreated group, as compared to saline liver transplant controls.CONCLUSION: HO-1 overexpression can provide potent protection against cold I/R injury. This effect depends, at least in part, on HO-1-mediated inhibition of antiapoptotic mechanism. 展开更多
关键词 Aged liver Ischemia-reperfusion injury Heme oxygenase-1
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Soil Organic Matter Dynamics Along a Vertical Vegetation Gradient in the Gongga Mountain on the Tibetan Plateau 被引量:17
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作者 LinWANG HuaOUYANG +3 位作者 Cai-PingZHOU fengzhang Ming-HuaSONG Yu-QiangTIAN 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第4期411-420,共10页
Abstract: Our knowledge about soil organic matter (SOM) dynamics is limited although this is an important issue in the study of responses of ecosystems to global climate changes. Twelve sampling plots were set up ever... Abstract: Our knowledge about soil organic matter (SOM) dynamics is limited although this is an important issue in the study of responses of ecosystems to global climate changes. Twelve sampling plots were set up every 200 m from 1 700 to 3 900 m along the vertical vegetation gradient along the east slope of Gongga Mountain. Samples were taken from all 12 plots for SOM content measurement, although only 5 of the 12 plots were subjected to radiocarbon measurements. A radiocarbon isotope method and a time-dependent model were used to quantify the SOM dynamics and SOM turnover rates along the vertical vegetation gradient. The results showed that the SOM turnover rate decreased and turnover time increased with soil depth for all vegetation types. The litter layer turnover rates presented a clear trend along the gradient. The litter layer turnover rates decreased with an increase in elevation, except that the litter layer turnover rate of mixed forest was higher than that of evergreen forest. Climatic factors, such as temperature and precipitation, were the main factors influencing the surface soil carbon dynamics. The turnover rates of the subsoil (including the A, B, and C horizons in the soil profiles) along the vertical gradient had no clear trends. The SOM of subalpine shrub and meadow turned over more slowly than that of the forest types in almost all soil horizons. The characteristic of short roots distributing in the upper part of the soil profile leads to different SOM dynamics of shrub and meadow compared with the forest types. Coniferous and mixed forests were susceptible to carbon loss from the young carbon pool, but their long and big roots resulted in high Δ14C values of the deep soil profiles and increased the input of young carbon to the deep soil. In evergreen forest, the carbon cumulative ability from the B horizon to the C horizon was weak. The different vegetation types, together with their different modes of nutrient and carbon intake, may be the mechanism conditioning the subsoil organic matter dynamics. 展开更多
关键词 carbon cycle radiocarbon isotope Tibetan Plateau turnover rate turnover time
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Complete Genome Sequences of the SARS-CoV: the BJ Group (Isolates BJ01-BJ04) 被引量:1
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作者 ShengliBi E‘deQin +56 位作者 ZuyuanXu WeiLi JingWang YongWuHu YongLiu ShuminDuan JianfeiHu YujunHan JingXu YanLi YaoYi YongdongZhou WeiLin1 JieWen HongXu RuanLi ZizhangZhang HaiyanSun JinguiZhu ManYu BaochangFan QingfaWu WeiLin2 LinTang Bao’anYang GuoqingLi WenmingPeng WenjieLi TaoJiang YajunDeng BohuaLiu JianpingShi YongqiangDeng WeiWei HongLiu ZongzhongTong fengzhang YuZhang Cui‘eWang YuquanLi JiaYe YonghuaGan JiaJi XiaoyuLi XiangjunTian FushuangLu GangTan RuifuYang BinLiu SiqiLiu SonggangLi JunWang JianWang WuchunCao JunYu XiaopingDong HuanmingYang 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2003年第3期180-192,共13页
Beijing has been one of the epicenters attacked most severely by the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) since the first patient was diagnosed in one of the city's hospitals. We now... Beijing has been one of the epicenters attacked most severely by the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) since the first patient was diagnosed in one of the city's hospitals. We now report complete genome sequences of the BJ Group, including four isolates (Isolates BJ01, BJ02, BJ03, and BJ04) of the SARS-CoV.It is remarkable that all members of the BJ Group share a common haplotype, consisting of seven loci that differentiate the group from other isolates published to date. Among 42 substitutions uniquely identified from the BJ group, 32 are non-synonymous changes at the amino acid level. Rooted phylogenetic trees, proposed on the basis of haplotypes and other sequence variations of SARS-CoV isolates from Canada, USA, Singapore, and China, gave rise to different paradigms but positioned the BJ Group, together with the newly discovered GD01 (GD-Ins29) in the same clade, followed by the H-U Group (from Hong Kong to USA) and the H-T Group (from Hong Kong to Toronto), leaving the SP Group (Singapore) more distant. This result appears to suggest a possible transmission path from Guangdong to Beijing/Hong Kong, then to other countries and regions. 展开更多
关键词 SARS SARS-COV HAPLOTYPE SUBSTITUTION PHYLOGENY
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