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Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress 被引量:4
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作者 geng da-li LU Li-yuan +10 位作者 YAN Ming-jia SHEN Xiao-xia JIANG Li-juan LI Hai-yan WANG Li-ping YAN Yan XU Ji-di LI Cui-ying YU Jian-tao MA Feng-wang GUAN Qing-mei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第6期1280-1294,共15页
Water deficit is one of the main limiting factors for apple growth and production.Root architecture plays an important role in drought tolerance of plants.However,little is known about the molecular basis of root syst... Water deficit is one of the main limiting factors for apple growth and production.Root architecture plays an important role in drought tolerance of plants.However,little is known about the molecular basis of root system in apple trees under drought.In this study,we compared root morphology of two widely used rootstocks of apple(R3 and Malus sieversii)under drought.Our results suggested that M.sieversii is more tolerant to drought than R3,since M.sieversii had a higher ratio of root to shoot as well as root hydraulic conductivity under long-term drought conditions.We then performed whole-genome transcriptomic analysis to figure out the molecular basis of root responses in M.sieversii under drought.It was found that genes involved in transcription regulation,signaling or biosynthesis of hormones,and oxidative stress were differentially expressed under drought.Consistent with the gene expression profile,roots of M.sieversii had higher activities of peroxidase(POD)and superoxide dismutase(SOD)under drought,as well as higher content of abscisic acid(ABA)and lower content of auxin.Taken together,our results revealed the physiological and transcriptomic analyses of M.sieversii roots in response to drought. 展开更多
关键词 MALUS sieversii root architecture DROUGHT stress RNA-seq
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Factors affecting hydraulic conductivity and methods to measure in plants
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作者 geng da-li LI Lei +2 位作者 YANG Yu-sen MA Feng-wang GUAN Qing-mei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第2期310-315,共6页
The plant hydraulic network faces several challenges under drought stress. Hydraulic conductivity is one of the major indicators of the hydraulic network’s response to drought stress. Here, we review our current unde... The plant hydraulic network faces several challenges under drought stress. Hydraulic conductivity is one of the major indicators of the hydraulic network’s response to drought stress. Here, we review our current understanding of the factors directly affecting hydraulic conductivity and the methods used to measure it. 展开更多
关键词 hydraulic conductivity AQUAPORINS VESSEL vessel embolism
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冬季修剪调控新梢内植物激素影响金银花的生长与产量 被引量:2
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作者 耿达立 卢恒 +2 位作者 刘伟 郭兰萍 王晓 《中国中药杂志》 CAS CSCD 北大核心 2022年第14期3749-3755,共7页
对不同修剪方式下金银花的生长与基因表型进行研究,探讨不同修剪方式促进金银花优质高产的机制。在2020年与2021年冬季对三年生金银花进行不同程度的冬剪,随后研究其萌芽期侧芽与开花期新梢的生长表型、激素含量和基因表达。结果表明,... 对不同修剪方式下金银花的生长与基因表型进行研究,探讨不同修剪方式促进金银花优质高产的机制。在2020年与2021年冬季对三年生金银花进行不同程度的冬剪,随后研究其萌芽期侧芽与开花期新梢的生长表型、激素含量和基因表达。结果表明,金银花新梢长度、新梢花芽数、新梢节间长度、新梢节数等生长表型的高低顺序为重剪>轻剪>对照;开花期重剪组的金银花新梢顶芽中生长素与玉米素含量最高,轻剪组次之,对照组最低。萌芽期金银花侧芽的生长素与玉米素含量为对照组最高,轻剪组次之,重剪组最低。对开花期新梢顶芽的转录组分析发掘出包括Lj1A1163T36、Lj3A719T115、Lj7C657T7、Lj9C505T15、Lj9A505T70在内的一系列与生长素和细胞分裂素相关的差异表达基因。该研究发现不同修剪方式下金银花新梢的生长表型与其顶芽的激素含量差异有关,表明不同程度的金银花冬季修剪影响了金银花新梢内生长素与细胞分裂素的含量,并进一步调控了金银花新梢中与激素相关的基因表达,最终促进了金银花新梢的生长并提高金银花产量。 展开更多
关键词 金银花 冬季修剪 生长素 细胞分裂素 基因表达
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