期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Association between infection of different strains of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis 被引量:4
1
作者 WU Yan-min YAN Jie +1 位作者 CHEN Li-li gu zhi-yuan 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第2期121-131,共11页
Objective: The aim of this study was to investigate subgingival infection frequencies ofPorphyromonas gingivalis and Actinobacillus actinomycetemcomitans strains with genetic variation in Chinese chronic periodontit... Objective: The aim of this study was to investigate subgingival infection frequencies ofPorphyromonas gingivalis and Actinobacillus actinomycetemcomitans strains with genetic variation in Chinese chronic periodontitis (CP) patients and to evaluate its correlation with clinical parameters. Methods: Two multiplex polymerase chain reaction (PCR) assays were developed to detect the 16SrDNA, collagenase (prtC) and fimbria (fimA) genes of P. gingivalis and the 16SrDNA, leukotoxin (lktA) and fimbria-associated protein (fap) genes ofA. actinomycetemcomitans in 60 sulcus samples from 30 periodontal healthy subjects and in 122 subgingival plaque samples from 61 patients with CP. The PCR products were further T-A cloned and sent for nucleotide sequence analysis. Results: The 16SrDNA,prtC andfimA genes ofP. gingivalis were detected in 92.6%, 85.2% and 80.3% of the subgingival plaque samples respectively, while the 16SrDNA, lktA andfap genes ofA. actinomycetemcomitans were in 84.4%, 75.4% and 50.0% respectively. Nucleotide sequence analysis showed 98.62%-100% homology of the PCR products in these genes with the reported sequences. P. gingivalis strains with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ were predominant in deep pockets (〉6 mm) or in sites with attachment loss 〉5 mm than in shallow pockets (3-4 mm) or in sites with attachment loss 〈2 mm (P〈0.05). P. gingivalis strains withprtC+/fimA+ also showed higher frequency in gingival index (GI)=3 than in GI=1 group (P〈0.05). Conclusion: Infection ofP. gingivalis with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ correlates with periodontal destruction of CP in Chinese. Nonetheless P. gingivalis fim4, prtC genes and A. actinomycetem- comitans lktA gene are closely associated with periodontal destruction, while A. actinomycetemcomitansfap gene is not. 展开更多
关键词 Porphyromonas gingivalis Actinobacillus actinomycetemcomitans STRAIN PERIODONTITIS PCR
下载PDF
Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters 被引量:1
2
作者 WU Yan-min YAN Jie +2 位作者 CHEN Li-li SUN Wei-lian gu zhi-yuan 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第11期876-883,共8页
Objective: To detect the infection frequencies of different genotypes of Epstein-Barr virus (EBV) in subgingival samples from chronic periodontitis (CP) patients, and to discuss the correlation between infection with ... Objective: To detect the infection frequencies of different genotypes of Epstein-Barr virus (EBV) in subgingival samples from chronic periodontitis (CP) patients, and to discuss the correlation between infection with EBV and clinical pa-rameters. Methods: Nested-PCR assay was used to detect EBV-1 and EBV-2 in subgingival samples from 65 CP patients, 65 gingivitis patients and 24 periodontally healthy individuals. The amplicons were further identified by restriction fragment length polymorphism analysis (RFLP) with endonucleases Afa I and Stu I. Clinical parameters mainly included bleeding on probing (BOP), probing depth (PD), attachment loss (AL) in six sites of the dentition. Results: In CP patients, gingivitis and periodontally healthy individuals, the infection frequencies were 47.7%, 24.6% and 16.7% for EBV-1, and 15.4%, 7.7% and 0% for EBV-2, respectively. In 2 out of the 65 CP patients co-infection of EBV-1 and EBV-2 was found. The positive rate of EBV-1 in chronic periodontitis patients was higher than that in gingivitis patients (P=0.01) and periodontally healthy individuals (P=0.01). But no significant difference was shown in EBV-1 frequency between gingivitis patients and healthy individuals (P>0.05) or in EBV-2 frequency among the three groups (P>0.05). In CP patients, higher mean BOP value was found in EBV-1 or EBV-2 positive patients than that in EBV negative ones (P<0.01), but with no statistical difference in the mean PD or AL value between EBV positive and negative patients (P>0.05). After initial periodontal treatment, 12 out of the 21 EBV-1 positive CP patients did not show detectable EBV-1 in subgingival samples. Conclusion: nPCR plus RFLP analysis is a sensitive, specific and stable method to detect EBV-1 and EBV-2 in subgingival samples. Subgingival infection with EBV-1 is closely associated with chronic perio-dontitis. Infection of EBV in subgingival samples was correlated with BOP. 展开更多
关键词 PERIODONTITIS Polymerase chain reaction Epstein-Barr virus POLYMORPHISM Restriction fragment length
下载PDF
添加藜麦对面条食用品质的影响 被引量:1
3
作者 朱建飞 顾志媛 +1 位作者 唐海燕 常海军 《重庆工商大学学报(自然科学版)》 2020年第2期89-95,共7页
利用中心组合试验设计研究添加藜麦对面条食用品质的影响规律,在单因素试验的基础上,筛选确定藜麦添加比例(%)和水添加量(g)为主效因子;采用二因素三水平的中心组合试验对主效因子的交互作用进行分析,得出各因素对面条蒸煮后的吸水率、... 利用中心组合试验设计研究添加藜麦对面条食用品质的影响规律,在单因素试验的基础上,筛选确定藜麦添加比例(%)和水添加量(g)为主效因子;采用二因素三水平的中心组合试验对主效因子的交互作用进行分析,得出各因素对面条蒸煮后的吸水率、蒸煮损失率、拉伸长度及影响规律;结果表明:面条蒸煮损失率回归模型显著(P<0.05),水添加量(B)对蒸煮损失率有极显著影响(P<0.01);面条吸水性回归模型不显著(P>0.05),各因素对吸水性的影响都不显著(P>0.05);面条拉伸长度的回归模型显著(P<0.05),藜麦添加比例(A)对拉伸长度有极显著影响(P<0.01)。 展开更多
关键词 藜麦 面条 响应面分析 食用品质
下载PDF
性别和植入物内固定方式影响瘤段骨酒精灭活再植后的骨愈合
4
作者 田志超 顾志远 +6 位作者 王鑫 刘志勇 姚伟涛 王家强 张鹏 蔡启卿 葛红 《中国组织工程研究》 CAS 北大核心 2018年第31期4931-4936,共6页
背景:恶性骨肿瘤的瘤段骨酒精灭活再植治疗在临床长期有应用价值,但深入探讨再植后骨愈合情况的研究较少。目的:总结分析影响瘤段骨酒精灭活再植后骨愈合的因素。方法:纳入21例恶性骨肿瘤患者,其中男8例,女13例,年龄7-56岁,均接受瘤段... 背景:恶性骨肿瘤的瘤段骨酒精灭活再植治疗在临床长期有应用价值,但深入探讨再植后骨愈合情况的研究较少。目的:总结分析影响瘤段骨酒精灭活再植后骨愈合的因素。方法:纳入21例恶性骨肿瘤患者,其中男8例,女13例,年龄7-56岁,均接受瘤段骨酒精灭活再植治疗,其中采用钢板螺钉内固定17例,髓内针固定4例,随访观察骨愈合情况,分析影响瘤段骨灭活再植骨愈合的相关因素。结果与结论:(1)21例患者随访2-6年,平均3.8年;(2)骨愈合患者11例,其中男1例,女10例,平均年龄20.8岁,灭活再植骨长度17.36cm,均采用钢板螺钉内固定,平均骨愈合时间20个月;(3)骨未愈合患者10例,其中男7例,女3例,平均年龄20.5岁,灭活再植骨长度16.10 cm,4例采用髓内针内固定,6例采用钢板内固定;(4)骨愈合组与骨未愈合组平均年龄、平均灭活再植骨长度比较差异无显著性意义,两组性别、内固定方式比较差异有显著性意义(P <0.05);(5)结果表明,年龄、灭活再植骨长度对瘤段骨酒精灭活再植的骨愈合无显著影响,女性患者、钢板内固定是瘤段骨酒精灭活再植治疗后骨愈合的有利因素。 展开更多
关键词 灭活再植 骨愈合 恶性骨肿瘤 钢板 髓内钉 生物材料 骨肿瘤 组织工程
下载PDF
射线灭活再植术在四肢骨肉瘤保肢中的应用 被引量:4
5
作者 顾志远 王鑫 +3 位作者 姚伟涛 田志超 刘志勇 蔡启卿 《中国骨与关节杂志》 CAS 2019年第1期37-41,共5页
目的探讨瘤段骨射线灭活再植在四肢骨肉瘤切除重建中的早期临床效果。方法自2016年6~12月,我科应用瘤段骨射线灭活再植重建四肢骨肉瘤切除后的骨缺损共6例,男3例,女3例,平均年龄12.7 (11~15)岁。所有病例Enneking分期均为IIB期,肿瘤... 目的探讨瘤段骨射线灭活再植在四肢骨肉瘤切除重建中的早期临床效果。方法自2016年6~12月,我科应用瘤段骨射线灭活再植重建四肢骨肉瘤切除后的骨缺损共6例,男3例,女3例,平均年龄12.7 (11~15)岁。所有病例Enneking分期均为IIB期,肿瘤均位于膝关节周围,其中股骨中下段4例,胫骨中上段2例。截除的瘤段长度平均24.8 (18~35) cm。瘤段骨边界外切除后使用电子直线加速器-X线片前后等中心对穿照射灭活,剂量60 Gy,平均照射30 (25~40) min。灭活后瘤段骨均使用锁定加压钢板固定重建。结果所有病例均获得满意随访,平均随访12.8 (6~18)个月,均未发现复发。1例因肺转移于术后6个月死亡,1例于术后12个月发现肺转移,目前带瘤存活。5例存活病例中,除1例瘤段骨-自体骨断端末次随访时仍不全愈合外,另4例骨折端均获得愈合,平均愈合时间8.5 (6~10)个月。存活病例下肢功能评分(MSTS)平均24.6 (22~29)分。共有3例发生术后并发症,包括1例内固定断裂,2例骨关节灭活后膝关节强直,经二次手术均获得满意效果。结论瘤段骨射线灭活再植是重建四肢骨肉瘤切除后骨缺损的可行方法,具有效果好、安全性高的特点。 展开更多
关键词 X线 骨肉瘤 骨移植
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部