The moisture content of a road subgrade in cold regions will increase after freeze-thaw cycles,resulting in subgrade strength and stiffness losses.Electroosmosis is widely used in treating saturated soft soils to decr...The moisture content of a road subgrade in cold regions will increase after freeze-thaw cycles,resulting in subgrade strength and stiffness losses.Electroosmosis is widely used in treating saturated soft soils to decrease the moisture content.The induced moisture migration during electroosmosis in unsaturated soil is much more complex than that of saturated soil because of a series of nonlinear changes in soil properties.This study first uses an exponential function to characterize the relationship between electroosmotic permeability and saturation degree.Then,a one-dimensional model is developed to simulate the electroosmosis-induced moisture migration in unsaturated soil.Simulation results show that electroosmosis reduces the saturation degree of the unsaturated soil,indicating that it can be applied to subgrade dewatering.Key parameters such as soil pore size distribution coefficient,air entry value,and effective voltage significantly affect moisture migration.Electroosmotic properties of unsaturated soils are extremely important to the efficiency of electroosmosis.展开更多
The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regu...The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regulation and prognosis,particularly those of hepatocellular carcinoma(HCC),remains unclear.To address this knowledge gap,we analyzed expression profiles obtained from The Cancer Genome Atlas(TCGA)database,comparing normal and malignant tumor tissues.We found that NRAV expression is significantly upregulated in tumor tissues compared to adjacent nontumor tissues.Kaplan-Meier(K-M)analysis revealed the prognostic power of NRAV,wherein overexpression was significantly linked to reduced overall survival in a diverse range of tumor patients.Furthermore,noteworthy associations were observed between NRAV,immune checkpoints,immune cell infiltration,genes related to autophagy,epithelial-mesenchymal transition(EMT),pyroptosis,tumor mutational burden(TMB),and microsatellite instability(MSI)across different cancer types,including HCC.Moreover,NRAV upregulation expression was associated with multiple pathological stages by clinical observations.Furthermore,our investigation revealed a substantial elevation in the expression of NRAV in both HCC tumor tissues and cells compared to normal tissues and cells.The inhibition of NRAV resulted in the inhibition of cell proliferation,migration,and invasion in HCC cells,while also influencing the expression of CD274(PD-L1)and CD44,along with various biomarkers associated with EMT,autophagy,and pyroptosis.The aforementioned results propose NRAV as a promising prognostic biomarker for HCC.展开更多
基金the financial support from the National Key Research and Development Program of China(No.2018YFC1505306)the National Natural Science Foundation of China(No.41971076).
文摘The moisture content of a road subgrade in cold regions will increase after freeze-thaw cycles,resulting in subgrade strength and stiffness losses.Electroosmosis is widely used in treating saturated soft soils to decrease the moisture content.The induced moisture migration during electroosmosis in unsaturated soil is much more complex than that of saturated soil because of a series of nonlinear changes in soil properties.This study first uses an exponential function to characterize the relationship between electroosmotic permeability and saturation degree.Then,a one-dimensional model is developed to simulate the electroosmosis-induced moisture migration in unsaturated soil.Simulation results show that electroosmosis reduces the saturation degree of the unsaturated soil,indicating that it can be applied to subgrade dewatering.Key parameters such as soil pore size distribution coefficient,air entry value,and effective voltage significantly affect moisture migration.Electroosmotic properties of unsaturated soils are extremely important to the efficiency of electroosmosis.
基金funded by China National Natural Youth Science Foundation(81802078)Zhejiang Province Public Welfare Research Foundation(GF20H200021)Zhejiang Provincial Department of Medicine and Health Foundation(2019RC315).
文摘The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regulation and prognosis,particularly those of hepatocellular carcinoma(HCC),remains unclear.To address this knowledge gap,we analyzed expression profiles obtained from The Cancer Genome Atlas(TCGA)database,comparing normal and malignant tumor tissues.We found that NRAV expression is significantly upregulated in tumor tissues compared to adjacent nontumor tissues.Kaplan-Meier(K-M)analysis revealed the prognostic power of NRAV,wherein overexpression was significantly linked to reduced overall survival in a diverse range of tumor patients.Furthermore,noteworthy associations were observed between NRAV,immune checkpoints,immune cell infiltration,genes related to autophagy,epithelial-mesenchymal transition(EMT),pyroptosis,tumor mutational burden(TMB),and microsatellite instability(MSI)across different cancer types,including HCC.Moreover,NRAV upregulation expression was associated with multiple pathological stages by clinical observations.Furthermore,our investigation revealed a substantial elevation in the expression of NRAV in both HCC tumor tissues and cells compared to normal tissues and cells.The inhibition of NRAV resulted in the inhibition of cell proliferation,migration,and invasion in HCC cells,while also influencing the expression of CD274(PD-L1)and CD44,along with various biomarkers associated with EMT,autophagy,and pyroptosis.The aforementioned results propose NRAV as a promising prognostic biomarker for HCC.
