口蹄疫(Foot and mouth disease,FMD)是一种全球性的、感染偶蹄动物的传染病。病毒衣壳对温度,pH敏感,容易在疫苗生产及储存过程中裂解为免疫原性较低的五聚体,影响疫苗的质量。口蹄疫病毒(Foot and mouth disease virus,FMDV)对热敏感...口蹄疫(Foot and mouth disease,FMD)是一种全球性的、感染偶蹄动物的传染病。病毒衣壳对温度,pH敏感,容易在疫苗生产及储存过程中裂解为免疫原性较低的五聚体,影响疫苗的质量。口蹄疫病毒(Foot and mouth disease virus,FMDV)对热敏感的机制研究对于疫苗抗原稳定性意义重大。本文通过循环热应激驯化方式诱导基因突变向耐热性发展,经噬斑纯化获得了驯化毒株M3,M9和M10。通过多序列比对分析发现驯化毒株P1区发生氨基酸突变,一步生长曲线表明驯化毒株氨基酸突变基本不影响病毒的复制,而驯化毒株相比野生型毒株衣壳耐热性有显著提高。驯化毒株M10单一位点突变(A1013T)增强衣壳稳定性,表明A1013T位点对于O型FMDV毒株衣壳耐热性的重要性,但机制仍待后期进一步阐明。该研究将为制备耐热性的FMD疫苗提供了理论依据。展开更多
The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In...The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV.展开更多
文摘口蹄疫(Foot and mouth disease,FMD)是一种全球性的、感染偶蹄动物的传染病。病毒衣壳对温度,pH敏感,容易在疫苗生产及储存过程中裂解为免疫原性较低的五聚体,影响疫苗的质量。口蹄疫病毒(Foot and mouth disease virus,FMDV)对热敏感的机制研究对于疫苗抗原稳定性意义重大。本文通过循环热应激驯化方式诱导基因突变向耐热性发展,经噬斑纯化获得了驯化毒株M3,M9和M10。通过多序列比对分析发现驯化毒株P1区发生氨基酸突变,一步生长曲线表明驯化毒株氨基酸突变基本不影响病毒的复制,而驯化毒株相比野生型毒株衣壳耐热性有显著提高。驯化毒株M10单一位点突变(A1013T)增强衣壳稳定性,表明A1013T位点对于O型FMDV毒株衣壳耐热性的重要性,但机制仍待后期进一步阐明。该研究将为制备耐热性的FMD疫苗提供了理论依据。
文摘The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV.
