Supercritical fluid chromatography(SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the a...Supercritical fluid chromatography(SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development.展开更多
A novel flow-injection chemiluminescence(FI-CL) method free of CL reagent was developed for the determination of captopril based on its enhancing effect on the CL derived from diperiodatoargentate(Ⅲ)-sulfuric acid sy...A novel flow-injection chemiluminescence(FI-CL) method free of CL reagent was developed for the determination of captopril based on its enhancing effect on the CL derived from diperiodatoargentate(Ⅲ)-sulfuric acid system. Compared with the conventional CL system, the CL system based on trivalent silver was characterized of good selectivity for the absence of CL reagent. The CL mechanism was discussed through CL spectra and UV–vis absorption spectra. The conditions of the FI-CL system were investigated and optimized.Under the optimal conditions, the relative CL intensity was linear with the captopril concentration in the range of 0.3–15.0 μg/m L. The detection limit for captopril was 0.05 μg/m L, and the relative standard deviation(n=11) was 2.0% for 5.0 μg/m L captopril. The proposed method was applied to the analysis of captopril in tablet and human urine with the recoveries of 83.1%–112.5%, and the relative standard deviations of 0.5%–4.4%. The results obtained by the proposed method agreed well with those obtained from HPLC method. The proposed method is fast, convenient, and cost-effective for the determination of captopril in medicine and biological samples.展开更多
Electrogenerated chemiluminescence, also known as electrochemiluminescence, abbreviated ECL, is a new technology combining electrochemistry and chemiluminescence. It is generated by high-energy electrons generated on ...Electrogenerated chemiluminescence, also known as electrochemiluminescence, abbreviated ECL, is a new technology combining electrochemistry and chemiluminescence. It is generated by high-energy electrons generated on the surface of the electrode in the emission process of excited state photons formed in the transfer process, and is a perfect combination of electrochemistry and spectroscopy. It not only has the advantages of good environment, high luminosity and wide dynamic range, but also has the characteristics of simple, stable and practical electrochemical methods, and nearly zero background signals. With the rapid development of nanomaterials, due to their unique electrical properties, large specific surface area, good biocompatibility and other characteristics, various nanomaterials have been widely used in the field of biosensors and sensitive detection. This review presented a general description of the research status of four different types of biosensors from the last decade years, summarized the application forms of nanomaterials in ECL biosensor, and outlines the building patterns and application example of the four main types of biosensors.展开更多
In this work, we report a facile synthesis method for NDs-Au@AgNPs surface-enhanced Raman scattering(SERS) substrate using Au seeds prepared by nitrogen-rich quantum dots(NDs). As a new member of quantum-dots family, ...In this work, we report a facile synthesis method for NDs-Au@AgNPs surface-enhanced Raman scattering(SERS) substrate using Au seeds prepared by nitrogen-rich quantum dots(NDs). As a new member of quantum-dots family, NDs are rich in nitrogen and oxygen-containing functional groups and therefore can be used as an efficient reducing and stabilizing agent for the synthesis of AuNPs. The NDs-Au@AgNPs with core-shell structures was synthesized by seed-mediated growth method in presence of NDs-AuNPs seeds and exhibited high SERS activity with a high SERS enhancement factor of 10~7. The NDs-Au@AgNPs SERS substrate was effectively used for the recognition and detection of isomers of meta, ortho and para nitroanilines, while commercial SERS substrate cannot. In addition, the possible adsorption behavior was further studied, which is of great significance for the related work about differentiation of isomers and the mechanism of SERS enhancement.展开更多
Bimetallic nanoparticles modified hollow-structured nanoporous carbons(NPCs) have been fabricated via a convenient one-step carbonizing strategy derived from covalent organic framework.The Pd/Fe/NPCs,Pt/Fe/NPCs and Rh...Bimetallic nanoparticles modified hollow-structured nanoporous carbons(NPCs) have been fabricated via a convenient one-step carbonizing strategy derived from covalent organic framework.The Pd/Fe/NPCs,Pt/Fe/NPCs and Rh/Fe/NPCs were obtained and can be used as Fenton-like catalysts with good stability and reusability.The catalytic activity was evaluated by the degradation of2,4-dichlorophenl(2,4-DCP).These fabricated bimetallic catalysts exhibited much higher catalytic activity than Fe/NPCs at room temperature.The enhancement of catalytic ability was benefited from synergetic catalytic effect of bimetallic nanoparticles and accelerated mass transfer of hollow structure.Additionally,the enhanced catalytic mechanism of bimetallic catalysts was studied in detail and the reasonable reaction pathway was proposed.Besides,the bimetallic catalysts were successfully used for degradation of 2,4-DCP in actual industrial wastewater and the removal efficiency could reach 74.3%within 120 min,which demonstrated the promising potential application of bimetallic catalysts in the removal of pollutants in environment.展开更多
Bisphenol A(BPA) was one of the environmental hormones that would cause endocrine and metabolic disorders in human or wildlife. This paper proposed a method to detect the trace amounts of BPA in water samples by fully...Bisphenol A(BPA) was one of the environmental hormones that would cause endocrine and metabolic disorders in human or wildlife. This paper proposed a method to detect the trace amounts of BPA in water samples by fully utilizing the enrichment and resonance amplification functions of a new dual-functional membrane. In this work, gold nanoparticles(AuNPs) modified by 3-amino-5-mercapto-1,2,4-triazole(AMT) were embedded in nylon66 membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing BPA molecules from water and achieve resonance amplification by connecting BPA to the surfaces of AuNPs. By designing an automatic sampler for large-volume enrichment, the SERS enhancement factor(EF) of the method was further improved to 1.2×10~5. The present method had been successfully applied to detect BPA in drinking water and environmental water by SERS with the detection limit of 0.012 μg/L. It had the potential for on-site detecting of BPA in various water samples.展开更多
Nowadays, DNA-mediated metal nanomaterials have received more and more attention due to their distinctive properties of easy synthesis, high stability and good biocompatibility. As a kind of efficient template to medi...Nowadays, DNA-mediated metal nanomaterials have received more and more attention due to their distinctive properties of easy synthesis, high stability and good biocompatibility. As a kind of efficient template to mediate the precise synthesis of metal nanomaterials, DNA can not only increase their recognition and biocompatibility, but also precisely control their morphology, structure and further analytical performance. DNA-mediated metal nanomaterials as ultrasensitive and selective probes have unique advantages for in situ rapid analysis of targets in complex samples. Herein, we reviewed the recent progress on synthesis strategies, morphologies, specific properties of DNA-mediated metal nanomaterials including nanoparticles and nanoclusters. Moreover, the applications of DNA-mediated metal nanomaterials to environmental and biological analysis were also summarized. Finally, the developing prospect of DNA-mediated metal nanomaterials in analytical chemistry was discussed and proposed.展开更多
Surface-enhanced Raman scattering(SERS)spectroscopy has been employed as a rapid analysis technology for food security inspection recently.Nowadays,it is still a great challenge to rapidly quantify multiple trace anti...Surface-enhanced Raman scattering(SERS)spectroscopy has been employed as a rapid analysis technology for food security inspection recently.Nowadays,it is still a great challenge to rapidly quantify multiple trace antibiotics potentially abused in aquaculture industry.In this work,a magnetic Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag substrate was prepared for the development of a reliable rapid SERS quantification method for multiple trace sulfonamides in aquatic products.This magnetic substrate had good uniformity,reproducibility,stability and SERS activity.Moreover,this substrate could integrate the magnetic separation-enrichment and matrix clean-up without cross contamination,which endowed it with good selectivity and antiinterference capability during real sample analysis.The electromagnetic enhancement and chemical enhancement mechanism of this magnetic substrate were studied in detail to reveal its good separationenrichment performance and SERS activity.Finally,a rapid SERS quantification method was established and practically applied for trace phthalic sulfathiazole(PST)and silver sulfadiazine(SSD)in aquatic products by using Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag magnetic substrates.Trace PST and SSD could be actually detected and quantified as 55.9μg/kg and 64.0μg/kg in aquatic products,respectively.Good recoveries of 83.9%-116%with relative standard deviations(RSDs)of 0.5%-3.2%for PST and 80.2%-102%with RSDs of 1.3%-5.8%for SSD were obtained.This work proposed an efficient and reliable method for rapid quantification of trace multiple sulfonamides in complex aquatic samples during food security inspection.展开更多
Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versat...Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versatile method was developed to fabricate flexible conjugated microporous polymer films for fast detecting the total quantity of developing agents and their oxidation products.Conjugated microporous polymer films were synthesized through in situ one-step approach by Suzuki coupling reaction.With such merits such as intense luminescence,easy synthesis and disposability,the polymer films were employed to detect developers based on fluorescence quenching in the printing wastewater.A linear response to p-benzoquinone,oxidation product of developers,was obtained in the range of 0.05-0.5 lmolL-1,with a detection limit of 0.015 lmolL-1.The strategy for in situ growth of flexible polyphenylene-conjugated microporous polymer(PP-CMP)films seems to be a very portable and general method for meeting different analytic purposes.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 21775047)Pearl River S and T Nova Program of Guangzhou, China (Grant No. 201806010055)the Fundamental Research Funds for the Central Universities (Grant No. 2018MS55)
文摘Supercritical fluid chromatography(SFC) meets with great favor due to its high efficiency, low organic solvent consumption, and the specialty for the identification of the isomeric species. This review describes the advances of SFC in targeted and untargeted lipid profiling. The advancement of the SFC instruments and the stationary phases are summarized. Typical applications of SFC to the targeted and untargeted lipid profiling are discussed in detail. Moreover, the perspectives of SFC in the lipid profiling are also proposed. As a useful and promising tool for investigating lipids in vitro and in vivo, SFC will predictably obtain further development.
基金supported by the National Natural Science Foundation of China (Nos. 21475153, 21575167, 21675178 and 21605163)the Guangdong Provincial Natural Science Foundation (Nos. 2015A030311020 and 2016A030313358)+1 种基金the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province (No.2015A030401036)the Guangzhou Science and Technology Program (No. 201604020165)
文摘A novel flow-injection chemiluminescence(FI-CL) method free of CL reagent was developed for the determination of captopril based on its enhancing effect on the CL derived from diperiodatoargentate(Ⅲ)-sulfuric acid system. Compared with the conventional CL system, the CL system based on trivalent silver was characterized of good selectivity for the absence of CL reagent. The CL mechanism was discussed through CL spectra and UV–vis absorption spectra. The conditions of the FI-CL system were investigated and optimized.Under the optimal conditions, the relative CL intensity was linear with the captopril concentration in the range of 0.3–15.0 μg/m L. The detection limit for captopril was 0.05 μg/m L, and the relative standard deviation(n=11) was 2.0% for 5.0 μg/m L captopril. The proposed method was applied to the analysis of captopril in tablet and human urine with the recoveries of 83.1%–112.5%, and the relative standard deviations of 0.5%–4.4%. The results obtained by the proposed method agreed well with those obtained from HPLC method. The proposed method is fast, convenient, and cost-effective for the determination of captopril in medicine and biological samples.
基金supported by the National Natural Science Foundation of China (Nos. 21675178, 21575167 and 21775167)the Guangdong Provincial Natural Science Foundation of China (No. 2016A030313358)+1 种基金the Research and Development Plan for Key Areas of Food Safety in Guangdong Province of China (No. 2019B020211001)the Guangzhou Science and Technology Program of China (No. 201604020165)
文摘Electrogenerated chemiluminescence, also known as electrochemiluminescence, abbreviated ECL, is a new technology combining electrochemistry and chemiluminescence. It is generated by high-energy electrons generated on the surface of the electrode in the emission process of excited state photons formed in the transfer process, and is a perfect combination of electrochemistry and spectroscopy. It not only has the advantages of good environment, high luminosity and wide dynamic range, but also has the characteristics of simple, stable and practical electrochemical methods, and nearly zero background signals. With the rapid development of nanomaterials, due to their unique electrical properties, large specific surface area, good biocompatibility and other characteristics, various nanomaterials have been widely used in the field of biosensors and sensitive detection. This review presented a general description of the research status of four different types of biosensors from the last decade years, summarized the application forms of nanomaterials in ECL biosensor, and outlines the building patterns and application example of the four main types of biosensors.
基金supported by the National Natural Science Foundation of China(Nos. 21675178, 21475153 and 21575167)the Natural Science Foundation of Guangdong Province, China (No. 2016A030313358)+1 种基金the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China (No. 2015A030401036)the Guangzhou Science and Technology Program of China (No. 201604020165),respectively
文摘In this work, we report a facile synthesis method for NDs-Au@AgNPs surface-enhanced Raman scattering(SERS) substrate using Au seeds prepared by nitrogen-rich quantum dots(NDs). As a new member of quantum-dots family, NDs are rich in nitrogen and oxygen-containing functional groups and therefore can be used as an efficient reducing and stabilizing agent for the synthesis of AuNPs. The NDs-Au@AgNPs with core-shell structures was synthesized by seed-mediated growth method in presence of NDs-AuNPs seeds and exhibited high SERS activity with a high SERS enhancement factor of 10~7. The NDs-Au@AgNPs SERS substrate was effectively used for the recognition and detection of isomers of meta, ortho and para nitroanilines, while commercial SERS substrate cannot. In addition, the possible adsorption behavior was further studied, which is of great significance for the related work about differentiation of isomers and the mechanism of SERS enhancement.
基金supported by the National Natural Science Foundation of China (Nos.21675178,21775167 and 21976213)the National Key Research and Development Program of China(No.2019YFC1606101)the Research and Development Plan for Key Areas of Food Safety in Guangdong Province of China (No.2019B020211001),respectively。
文摘Bimetallic nanoparticles modified hollow-structured nanoporous carbons(NPCs) have been fabricated via a convenient one-step carbonizing strategy derived from covalent organic framework.The Pd/Fe/NPCs,Pt/Fe/NPCs and Rh/Fe/NPCs were obtained and can be used as Fenton-like catalysts with good stability and reusability.The catalytic activity was evaluated by the degradation of2,4-dichlorophenl(2,4-DCP).These fabricated bimetallic catalysts exhibited much higher catalytic activity than Fe/NPCs at room temperature.The enhancement of catalytic ability was benefited from synergetic catalytic effect of bimetallic nanoparticles and accelerated mass transfer of hollow structure.Additionally,the enhanced catalytic mechanism of bimetallic catalysts was studied in detail and the reasonable reaction pathway was proposed.Besides,the bimetallic catalysts were successfully used for degradation of 2,4-DCP in actual industrial wastewater and the removal efficiency could reach 74.3%within 120 min,which demonstrated the promising potential application of bimetallic catalysts in the removal of pollutants in environment.
基金supported by the National Natural Science Foundation of China(Nos. 21575168, 21475153,21575167 and 21675178)the Guangdong Provincial Natural Science Foundation of China(No. 2015A030311020)+1 种基金the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China(No. 2015A030401036)the Guangzhou Science andTechnology Program of China(Nos.201604020165, 201704020040)
文摘Bisphenol A(BPA) was one of the environmental hormones that would cause endocrine and metabolic disorders in human or wildlife. This paper proposed a method to detect the trace amounts of BPA in water samples by fully utilizing the enrichment and resonance amplification functions of a new dual-functional membrane. In this work, gold nanoparticles(AuNPs) modified by 3-amino-5-mercapto-1,2,4-triazole(AMT) were embedded in nylon66 membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing BPA molecules from water and achieve resonance amplification by connecting BPA to the surfaces of AuNPs. By designing an automatic sampler for large-volume enrichment, the SERS enhancement factor(EF) of the method was further improved to 1.2×10~5. The present method had been successfully applied to detect BPA in drinking water and environmental water by SERS with the detection limit of 0.012 μg/L. It had the potential for on-site detecting of BPA in various water samples.
基金supported by the National Natural Science Foundation of China(Nos.21475154,21475153 and 21675178)the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China(No. 2015A030401036)the Guangzhou Science and Technology Program of China(No. 201604020165),respectively
文摘Nowadays, DNA-mediated metal nanomaterials have received more and more attention due to their distinctive properties of easy synthesis, high stability and good biocompatibility. As a kind of efficient template to mediate the precise synthesis of metal nanomaterials, DNA can not only increase their recognition and biocompatibility, but also precisely control their morphology, structure and further analytical performance. DNA-mediated metal nanomaterials as ultrasensitive and selective probes have unique advantages for in situ rapid analysis of targets in complex samples. Herein, we reviewed the recent progress on synthesis strategies, morphologies, specific properties of DNA-mediated metal nanomaterials including nanoparticles and nanoclusters. Moreover, the applications of DNA-mediated metal nanomaterials to environmental and biological analysis were also summarized. Finally, the developing prospect of DNA-mediated metal nanomaterials in analytical chemistry was discussed and proposed.
基金financially supported by the National Natural Science Foundation of China(Nos.22074161 and 21976213)Guangdong Basic and Applied Basic Research Foundation(No.2019A1515010107)+3 种基金the Science and Technology Planning Project of Guangzhou City(No.202102080167)the Research and Development Plan for Key Areas of Food Safety in Guangdong Province of China(No.2019B020211001)the National Key Research and Development Program of China(No.2019YFC1606101)the State Key Program of National Natural Science of China(No.22134007)。
文摘Surface-enhanced Raman scattering(SERS)spectroscopy has been employed as a rapid analysis technology for food security inspection recently.Nowadays,it is still a great challenge to rapidly quantify multiple trace antibiotics potentially abused in aquaculture industry.In this work,a magnetic Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag substrate was prepared for the development of a reliable rapid SERS quantification method for multiple trace sulfonamides in aquatic products.This magnetic substrate had good uniformity,reproducibility,stability and SERS activity.Moreover,this substrate could integrate the magnetic separation-enrichment and matrix clean-up without cross contamination,which endowed it with good selectivity and antiinterference capability during real sample analysis.The electromagnetic enhancement and chemical enhancement mechanism of this magnetic substrate were studied in detail to reveal its good separationenrichment performance and SERS activity.Finally,a rapid SERS quantification method was established and practically applied for trace phthalic sulfathiazole(PST)and silver sulfadiazine(SSD)in aquatic products by using Ti_(3)C_(2)T_(x)/Fe_(3)O_(4)/Ag magnetic substrates.Trace PST and SSD could be actually detected and quantified as 55.9μg/kg and 64.0μg/kg in aquatic products,respectively.Good recoveries of 83.9%-116%with relative standard deviations(RSDs)of 0.5%-3.2%for PST and 80.2%-102%with RSDs of 1.3%-5.8%for SSD were obtained.This work proposed an efficient and reliable method for rapid quantification of trace multiple sulfonamides in complex aquatic samples during food security inspection.
基金The work were supported by the National Natural Science Foundation of China(Nos.21575168,21277176,21475153 and 21675178)the Guangdong Provincial Natural Science Foundation of China(No.2015A030311020)+1 种基金the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China(No.2015A030401036)the Guangzhou Science and Technology Program of China(No.201604020165),respectively。
文摘Conjugated microporous polymers(CMPs)are a kind of porous skeleton polymers,which are characterized by extended conjugated skeletons,large specific surface area and structure stability.In this work,a facile and versatile method was developed to fabricate flexible conjugated microporous polymer films for fast detecting the total quantity of developing agents and their oxidation products.Conjugated microporous polymer films were synthesized through in situ one-step approach by Suzuki coupling reaction.With such merits such as intense luminescence,easy synthesis and disposability,the polymer films were employed to detect developers based on fluorescence quenching in the printing wastewater.A linear response to p-benzoquinone,oxidation product of developers,was obtained in the range of 0.05-0.5 lmolL-1,with a detection limit of 0.015 lmolL-1.The strategy for in situ growth of flexible polyphenylene-conjugated microporous polymer(PP-CMP)films seems to be a very portable and general method for meeting different analytic purposes.
