The diffuse brain injury model was conducted in Sprague-Dawley rats, according to Marmarou's free-fall attack. The water content in brain tissue, expression of metabotropic glutamate receptor 1α mRNA and protein ...The diffuse brain injury model was conducted in Sprague-Dawley rats, according to Marmarou's free-fall attack. The water content in brain tissue, expression of metabotropic glutamate receptor 1α mRNA and protein were significantly increased after injury, reached a peak at 24 hours, and then gradually decreased. After treatment with the competitive antagonist of metabotropic glutamate receptor 1α, (RS)-1-aminoindan-1, 5-dicarboxylic acid, the water content of brain tissues decreased between 12-72 hours after injury, and neurological behaviors improved at 2 weeks. These experimental findings suggest that the 1-aminoindan-1, 5-dicarboxylic acid may result in marked neuroprotection against diffuse brain injury.展开更多
The present study established a rat cortical neuronal model of in vitro mechanical injury.At 30 mi-nutes after injury,the survival rate of the injured cortical neurons was decreased compared with normal neurons,and wa...The present study established a rat cortical neuronal model of in vitro mechanical injury.At 30 mi-nutes after injury,the survival rate of the injured cortical neurons was decreased compared with normal neurons,and was gradually decreased with aggravated degree of injury.Reverse transcription-polymerase chain reaction results showed that at 1 hour after injury,there was increased expression of metabotropic glutamate receptor 1a in cortical neurons.Immunohistochemical staining results showed that at 30 minutes after injury,the number of metabotropic glutamate receptor 1a-positive cells increased compared with normal neurons.At 12 hours after injury,lactate dehydrogenase activity in the (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA)-treated injury neurons was significantly decreased than that in the pure injury group.At 1 hour after injury,intracellular free Ca2+ concentration was markedly decreased in the AIDA-treated injury neurons than that in the pure injury neurons.These findings suggest that after mechanical injury to cortical neurons,metabotropic glutamate receptor 1a expression increased.The resulting increase in intracellular free Ca2+ concentration was blocked by AIDA,indicating that AIDA exhibits neuroprotective effects after mechanical injury.展开更多
文摘The diffuse brain injury model was conducted in Sprague-Dawley rats, according to Marmarou's free-fall attack. The water content in brain tissue, expression of metabotropic glutamate receptor 1α mRNA and protein were significantly increased after injury, reached a peak at 24 hours, and then gradually decreased. After treatment with the competitive antagonist of metabotropic glutamate receptor 1α, (RS)-1-aminoindan-1, 5-dicarboxylic acid, the water content of brain tissues decreased between 12-72 hours after injury, and neurological behaviors improved at 2 weeks. These experimental findings suggest that the 1-aminoindan-1, 5-dicarboxylic acid may result in marked neuroprotection against diffuse brain injury.
文摘The present study established a rat cortical neuronal model of in vitro mechanical injury.At 30 mi-nutes after injury,the survival rate of the injured cortical neurons was decreased compared with normal neurons,and was gradually decreased with aggravated degree of injury.Reverse transcription-polymerase chain reaction results showed that at 1 hour after injury,there was increased expression of metabotropic glutamate receptor 1a in cortical neurons.Immunohistochemical staining results showed that at 30 minutes after injury,the number of metabotropic glutamate receptor 1a-positive cells increased compared with normal neurons.At 12 hours after injury,lactate dehydrogenase activity in the (RS)-1-aminoindan-1,5-dicarboxylic acid (AIDA)-treated injury neurons was significantly decreased than that in the pure injury group.At 1 hour after injury,intracellular free Ca2+ concentration was markedly decreased in the AIDA-treated injury neurons than that in the pure injury neurons.These findings suggest that after mechanical injury to cortical neurons,metabotropic glutamate receptor 1a expression increased.The resulting increase in intracellular free Ca2+ concentration was blocked by AIDA,indicating that AIDA exhibits neuroprotective effects after mechanical injury.
