BACKGROUND: Most reports on the prognosis of cholecystectomy have been short-term studied, and few long-term reports have suggested variable incidences of common bile duct stones after cholecystectomy. We retrospectiv...BACKGROUND: Most reports on the prognosis of cholecystectomy have been short-term studied, and few long-term reports have suggested variable incidences of common bile duct stones after cholecystectomy. We retrospectively reviewed the data to find the possible association between cholecystectomy and the subsequent occurrence of primary common bile duct stones. METHODS: The data were reviewed retrospectively of 478 patients with primary common bile duct stones diagnosed and treated by endoscopic sphincterotomy at our hospitals between January 1994 and December 2003. RESULTS: Sixty-one (14.1%) of the 432 patients had a history of cholecystectomy, with an incidence rate markedly higher than that in the general population. The mean interval between cholecystectomy and the occurrence of primary common bile duct stones was 8.23 years, with the longest being 28 years and the shortest 2 years. Compared with the patients who had not undergone a prior cholecystectomy, those who had had a prior cholecystectomy more often accompanied with acute cholangiolitis (χ2=8.259, P<0.01), and multiple stones or sand-like stones were frequently found (χ2=9.030, P<0.01). CONCLUSIONS: These results suggest a possible relation-ship between cholecystectomy and the subsequent occurrence of primary common bile duct stones. Perhaps patients with primary common bile duct stones who have had a prior cholecystectomy have a higher probability of infection of the biliary system. The infection may be one of the causes of occurrence of primary common bile duct stones after cholecystectomy.展开更多
BACKGROUND:In experimental acute pancreatitis,a large amount of reactive oxygen species are produced,and in turn cytoskeletal changes may be induced in pancreatic tissue.These changes contribute to an imbalance of dig...BACKGROUND:In experimental acute pancreatitis,a large amount of reactive oxygen species are produced,and in turn cytoskeletal changes may be induced in pancreatic tissue.These changes contribute to an imbalance of digestive enzyme segregation,transport,exocytosis and activation, resulting in cell injury.In this study,we assessed the effects of chondroitin sulfate(CS)on attenuation of oxidative damage and protection of F-actin in rats with acute necrotizing pancreatitis(ANP). METHODS:Ninety male Wistar rats were divided randomly into three groups.Group A was infused with 5%sodium taurocholate;group B was treated with CS; and group C served as control.Rats from the three groups were killed at 1,3 or 8 hours.The levels were measured of malonyl dialdehyde(MDA),total superoxide dismutase (SOD),glutathione synthetase(GSH),serum amylase (SAM)and adenosine triphosphate(ATP).F-actin immunostained with rhodamine-phalloidin was analyzed using a confocal laser scanning system and the content of F-actin protein was determined. RESULTS:The levels of SAM increased in groups A and B,whereas the levels of GSH,SOD and ATP in group A decreased markedly during pancreatitis,and MDA increased significantly.The levels of GSH,SOD and ATP in group B were higher than those in group A,but the level of MDA was lower than in group A.At the same time, ANP resulted in early disruption of the cytoskeleton with dramatic changes and a loss of F-actin.Administration of CS moderated the damage to the actin cytoskeleton.CONCLUSIONS:Retrograde infusion of sodium taurocholate via the pancreatic duct may produce pancreatic necrosis and a marked increase in serum amylase activity,induce a severe depletion of ATP level, prime lipid peroxidation,and damage F-actin.Treatment with CS can ameliorate pancreatic cell conditions,limit cell membrane peroxidation,protect F-actin,and attenuate pancreatitis.展开更多
BACKGROUND:Since conventional methods are difficult to deal with pancreatic tumors close to the portal veins,we investigated the feasibility and norms for retrograde distal pancreatectomy by cutting the neck of the pa...BACKGROUND:Since conventional methods are difficult to deal with pancreatic tumors close to the portal veins,we investigated the feasibility and norms for retrograde distal pancreatectomy by cutting the neck of the pancreas first. METHOD:The clinical data and surgical procedures of retrograde distal pancreatectomy given to 11 patients from July 2001 to June 2007 were analyzed. RESULTS:All 11 operations were completed successfully. The mean time of the operation was 143±71 minutes.The mean volume of hemorrhage was 239 ml.The mean time of hospitalization was 12±4.2 days.No blood transfusion was needed during the operation,and all patients had a good postoperative recovery.No neopathy of diabetes mellitus, pancreatic fistula or other complications occurred after the operation.The follow-up of all patients(4-60 months) showed that 3 patients survived for 14,16 and 33 months, respectively,and the other patients are still alive. CONCLUSIONS:Retrograde distal pancreatectomy is useful for exposing the portal and superior mesenteric veins while avoiding operative injury.Interception of the blood supply of the spleen helps to reduce the volume of hemorrhage,while making the operation easier and the operative time short.It is advantageous for tumor resection of the body of the pancreas near the portal veins.展开更多
文摘BACKGROUND: Most reports on the prognosis of cholecystectomy have been short-term studied, and few long-term reports have suggested variable incidences of common bile duct stones after cholecystectomy. We retrospectively reviewed the data to find the possible association between cholecystectomy and the subsequent occurrence of primary common bile duct stones. METHODS: The data were reviewed retrospectively of 478 patients with primary common bile duct stones diagnosed and treated by endoscopic sphincterotomy at our hospitals between January 1994 and December 2003. RESULTS: Sixty-one (14.1%) of the 432 patients had a history of cholecystectomy, with an incidence rate markedly higher than that in the general population. The mean interval between cholecystectomy and the occurrence of primary common bile duct stones was 8.23 years, with the longest being 28 years and the shortest 2 years. Compared with the patients who had not undergone a prior cholecystectomy, those who had had a prior cholecystectomy more often accompanied with acute cholangiolitis (χ2=8.259, P<0.01), and multiple stones or sand-like stones were frequently found (χ2=9.030, P<0.01). CONCLUSIONS: These results suggest a possible relation-ship between cholecystectomy and the subsequent occurrence of primary common bile duct stones. Perhaps patients with primary common bile duct stones who have had a prior cholecystectomy have a higher probability of infection of the biliary system. The infection may be one of the causes of occurrence of primary common bile duct stones after cholecystectomy.
文摘BACKGROUND:In experimental acute pancreatitis,a large amount of reactive oxygen species are produced,and in turn cytoskeletal changes may be induced in pancreatic tissue.These changes contribute to an imbalance of digestive enzyme segregation,transport,exocytosis and activation, resulting in cell injury.In this study,we assessed the effects of chondroitin sulfate(CS)on attenuation of oxidative damage and protection of F-actin in rats with acute necrotizing pancreatitis(ANP). METHODS:Ninety male Wistar rats were divided randomly into three groups.Group A was infused with 5%sodium taurocholate;group B was treated with CS; and group C served as control.Rats from the three groups were killed at 1,3 or 8 hours.The levels were measured of malonyl dialdehyde(MDA),total superoxide dismutase (SOD),glutathione synthetase(GSH),serum amylase (SAM)and adenosine triphosphate(ATP).F-actin immunostained with rhodamine-phalloidin was analyzed using a confocal laser scanning system and the content of F-actin protein was determined. RESULTS:The levels of SAM increased in groups A and B,whereas the levels of GSH,SOD and ATP in group A decreased markedly during pancreatitis,and MDA increased significantly.The levels of GSH,SOD and ATP in group B were higher than those in group A,but the level of MDA was lower than in group A.At the same time, ANP resulted in early disruption of the cytoskeleton with dramatic changes and a loss of F-actin.Administration of CS moderated the damage to the actin cytoskeleton.CONCLUSIONS:Retrograde infusion of sodium taurocholate via the pancreatic duct may produce pancreatic necrosis and a marked increase in serum amylase activity,induce a severe depletion of ATP level, prime lipid peroxidation,and damage F-actin.Treatment with CS can ameliorate pancreatic cell conditions,limit cell membrane peroxidation,protect F-actin,and attenuate pancreatitis.
文摘BACKGROUND:Since conventional methods are difficult to deal with pancreatic tumors close to the portal veins,we investigated the feasibility and norms for retrograde distal pancreatectomy by cutting the neck of the pancreas first. METHOD:The clinical data and surgical procedures of retrograde distal pancreatectomy given to 11 patients from July 2001 to June 2007 were analyzed. RESULTS:All 11 operations were completed successfully. The mean time of the operation was 143±71 minutes.The mean volume of hemorrhage was 239 ml.The mean time of hospitalization was 12±4.2 days.No blood transfusion was needed during the operation,and all patients had a good postoperative recovery.No neopathy of diabetes mellitus, pancreatic fistula or other complications occurred after the operation.The follow-up of all patients(4-60 months) showed that 3 patients survived for 14,16 and 33 months, respectively,and the other patients are still alive. CONCLUSIONS:Retrograde distal pancreatectomy is useful for exposing the portal and superior mesenteric veins while avoiding operative injury.Interception of the blood supply of the spleen helps to reduce the volume of hemorrhage,while making the operation easier and the operative time short.It is advantageous for tumor resection of the body of the pancreas near the portal veins.
