Objective: To study the effect of MiR-377-mediated Syk on atherosclerosis in ApoE/mice. Methods: The experimental subjects were divided into control group (ApoE_/mice), MiR-377 group (ApoE_/mice injected with MiR-377)...Objective: To study the effect of MiR-377-mediated Syk on atherosclerosis in ApoE/mice. Methods: The experimental subjects were divided into control group (ApoE_/mice), MiR-377 group (ApoE_/mice injected with MiR-377), Syk group (ApoE_/mice injected with Syk) and Syk+ MiR-377 group (ApoE_/mice injected with MiR-377and Syk). MiR-377 single-stranded antisense nucleotide drugs and Syk gene sequences were prepared and transfected into ApoE_/mice. The expression of Syk gene and protein in ApoE_/mice was detected by qPCR and Western blot, and the levels of inflammatory factors, lipid and oxidative dismutase were detected. The effects of MiR-377 on atherosclerotic aortic plaque and surface area in ApoE_/mice were studied by HE staining and oil red O staining. Results: The relative expression of Syk gene in MiR-377 group was significantly lower than that in control group, and the expression of Syk protein in MiR-377 group was significantly lower than that in control group. TNF-alpha levels in Syk group were significantly lower than those in control group and MiR-377 group. The 1L-6 level in the MiR-377 group was significantly higher than that in the control group, while the 1L-6 level in the Syk group and the MiR377+Syk group was significantly lower than that in the control group. The level of 1L-1β in the MiR377 group was significantly lower than that in the control group. The TC level of MiR-377 group was significantly lower than that of control group, and the 1L-1β level of Syk group was significantly higher than that of control group and MiR-377 group. The level of LDL in the MiR-377 group was significantly lower than that in the control group, the level of HDL in the MiR-377 group was significantly lower than that in the control group, and the level of HDL in the Syk group was significantly higher than that in the control group and the MiR-377 group. The level of SOD in the MiR-377 group was significantly higher than that in the control group. There were obvious aortic plaques in the control group and Syk group. There were no obvious aortic plaques in the MiR-377+Syk group and the MiR-377 treated group. The area of atherosclerotic plaque in ApoE_/mice treated with MiR-377 decreased significantly. The plaque area of ApoE-/- mice treated with Syk increased. The ratio of sclerotic plaque area to total area was 25.63% in control group, 7.81% in mi-377 group, 35.19% in Syk group and 9.73% in Syk + MiR-377 group. Conclusion: Overexpression of Syk gene can increase lipid accumulation and oxidative stress in atherosclerotic cells. Mir-377 can regulate the development of atherosclerosis by inhibiting the expression of Syk gene.展开更多
Janus films with asymmetric physical/chemical properties have attracted con siderable attention due to their promising applications in personal thermal management,electronic skin s,sensors,actuators,etc.However,tradit...Janus films with asymmetric physical/chemical properties have attracted con siderable attention due to their promising applications in personal thermal management,electronic skin s,sensors,actuators,etc.However,traditional methods for fabricating Janus films conventionally need the assistance of an interface or auxiliary equipment,which are usually complex and time-consuming.Herein,flexible poly(vinyl alcohol)(PVA)/graphene oxide(GO)/h-BN(recorded as PVA/GO/h-BN)Janus films with thermally,optically,and electrically anisotropic properties are fabricated by a simple density deposition self-assem bly method,which just utilizes the density difference between GO and h-BN during water evaporation.Experimental results show that the two sides of the acquired Janus films have obvious asymmetric characteristics.In the original state of the PVA/GO/h-BN Janus films,the thermal conductivity of the GO side(10.06 W·m^(-1)·K^(-1))is generally lower than that of the h-BN side(10.48W·m^(-1)·K^(-1)).But after GO is reduced,the thermal conductivity of the rGO side reaches 12.17 W·m^(-1)·K^(-1),surpassing that of the h-BN side.In addition,the relative reflectance of the h-BN side of Janus film is also significantly higher than that of the rGO side,and the su rface resistance difference between the two sides is about 4 orders of magnitude.The prepared PVA/GO/h-BN Janus films show great application potential in human thermal management,light conversion switches,and electronic skins.This study provides a simple and versatile strategy for fabricating Janus films with multifunctional(such as thermal,optical,and electrical)anisotropies.展开更多
The pre-reduced Bayan Obo ferroniobium（FeNb）ore concentrate block was taken as raw materials for studying the physical properties of niobium-enriched slag and changes in niobium recovery rate.In addition,the dephosp...The pre-reduced Bayan Obo ferroniobium（FeNb）ore concentrate block was taken as raw materials for studying the physical properties of niobium-enriched slag and changes in niobium recovery rate.In addition,the dephosphorization rate of the slag under different melting-separation conditions was investigated using the melting-separation test.The research results demonstrate that（i）the niobium recovery rate and dephosphorization rate of the slag decrease with the increase in melting-separation temperature;（ii）the niobium recovery rate of the slag initially increases and then decreases with increase in basicity and time;and（iii）the dephosphorization rate of the slag increases with the increase in basicity and time.When the test was performed under the conditions of basicity of 0.6-0.7,time of 7-10min,and temperature of 1400-1450°C,the niobium recovery rate and dephosphorization rate are over 96%and 95%,respectively.By scanning electron microscopy,it is observed that niobium mainly exists in the form of calcium and titanium silicate within the slag phase,with uneven distribution.展开更多
基金Weifang Science and Technology Development Plan Project (2015WS004)
文摘Objective: To study the effect of MiR-377-mediated Syk on atherosclerosis in ApoE/mice. Methods: The experimental subjects were divided into control group (ApoE_/mice), MiR-377 group (ApoE_/mice injected with MiR-377), Syk group (ApoE_/mice injected with Syk) and Syk+ MiR-377 group (ApoE_/mice injected with MiR-377and Syk). MiR-377 single-stranded antisense nucleotide drugs and Syk gene sequences were prepared and transfected into ApoE_/mice. The expression of Syk gene and protein in ApoE_/mice was detected by qPCR and Western blot, and the levels of inflammatory factors, lipid and oxidative dismutase were detected. The effects of MiR-377 on atherosclerotic aortic plaque and surface area in ApoE_/mice were studied by HE staining and oil red O staining. Results: The relative expression of Syk gene in MiR-377 group was significantly lower than that in control group, and the expression of Syk protein in MiR-377 group was significantly lower than that in control group. TNF-alpha levels in Syk group were significantly lower than those in control group and MiR-377 group. The 1L-6 level in the MiR-377 group was significantly higher than that in the control group, while the 1L-6 level in the Syk group and the MiR377+Syk group was significantly lower than that in the control group. The level of 1L-1β in the MiR377 group was significantly lower than that in the control group. The TC level of MiR-377 group was significantly lower than that of control group, and the 1L-1β level of Syk group was significantly higher than that of control group and MiR-377 group. The level of LDL in the MiR-377 group was significantly lower than that in the control group, the level of HDL in the MiR-377 group was significantly lower than that in the control group, and the level of HDL in the Syk group was significantly higher than that in the control group and the MiR-377 group. The level of SOD in the MiR-377 group was significantly higher than that in the control group. There were obvious aortic plaques in the control group and Syk group. There were no obvious aortic plaques in the MiR-377+Syk group and the MiR-377 treated group. The area of atherosclerotic plaque in ApoE_/mice treated with MiR-377 decreased significantly. The plaque area of ApoE-/- mice treated with Syk increased. The ratio of sclerotic plaque area to total area was 25.63% in control group, 7.81% in mi-377 group, 35.19% in Syk group and 9.73% in Syk + MiR-377 group. Conclusion: Overexpression of Syk gene can increase lipid accumulation and oxidative stress in atherosclerotic cells. Mir-377 can regulate the development of atherosclerosis by inhibiting the expression of Syk gene.
基金financially supported by the National Natural Science Foundation of China(No.51373059)the Graphene Powder&Composite Materials Research Center of FujianXiamen Key Laboratory of Polymers and Electronic Materials。
文摘Janus films with asymmetric physical/chemical properties have attracted con siderable attention due to their promising applications in personal thermal management,electronic skin s,sensors,actuators,etc.However,traditional methods for fabricating Janus films conventionally need the assistance of an interface or auxiliary equipment,which are usually complex and time-consuming.Herein,flexible poly(vinyl alcohol)(PVA)/graphene oxide(GO)/h-BN(recorded as PVA/GO/h-BN)Janus films with thermally,optically,and electrically anisotropic properties are fabricated by a simple density deposition self-assem bly method,which just utilizes the density difference between GO and h-BN during water evaporation.Experimental results show that the two sides of the acquired Janus films have obvious asymmetric characteristics.In the original state of the PVA/GO/h-BN Janus films,the thermal conductivity of the GO side(10.06 W·m^(-1)·K^(-1))is generally lower than that of the h-BN side(10.48W·m^(-1)·K^(-1)).But after GO is reduced,the thermal conductivity of the rGO side reaches 12.17 W·m^(-1)·K^(-1),surpassing that of the h-BN side.In addition,the relative reflectance of the h-BN side of Janus film is also significantly higher than that of the rGO side,and the su rface resistance difference between the two sides is about 4 orders of magnitude.The prepared PVA/GO/h-BN Janus films show great application potential in human thermal management,light conversion switches,and electronic skins.This study provides a simple and versatile strategy for fabricating Janus films with multifunctional(such as thermal,optical,and electrical)anisotropies.
基金financially supported by the National Science and Technology Support Program (2008BAB32087)
文摘The pre-reduced Bayan Obo ferroniobium（FeNb）ore concentrate block was taken as raw materials for studying the physical properties of niobium-enriched slag and changes in niobium recovery rate.In addition,the dephosphorization rate of the slag under different melting-separation conditions was investigated using the melting-separation test.The research results demonstrate that（i）the niobium recovery rate and dephosphorization rate of the slag decrease with the increase in melting-separation temperature;（ii）the niobium recovery rate of the slag initially increases and then decreases with increase in basicity and time;and（iii）the dephosphorization rate of the slag increases with the increase in basicity and time.When the test was performed under the conditions of basicity of 0.6-0.7,time of 7-10min,and temperature of 1400-1450°C,the niobium recovery rate and dephosphorization rate are over 96%and 95%,respectively.By scanning electron microscopy,it is observed that niobium mainly exists in the form of calcium and titanium silicate within the slag phase,with uneven distribution.
