AIM: To investigate the effects of the WWOX gene on the human hepatic carcinoma cell line SMMC-7721. METHODS: Full-length WWOX cDNA was amplified from normal human liver tissues. Full-length cDNA was subcloned into pE...AIM: To investigate the effects of the WWOX gene on the human hepatic carcinoma cell line SMMC-7721. METHODS: Full-length WWOX cDNA was amplified from normal human liver tissues. Full-length cDNA was subcloned into pEGFP-N1, a eukaryotic expression vector. After introduction of the WWOX gene into cancer cells using liposomes, the WWOX protein level in the cells was detected through Western blotting. Cell growth rates were assessed by methyl thiazolyl tetrazolium (MTT) and colony formation assays. Cell cycle progression and cell apoptosis were measured by flow cytometry. The phosphorylated protein kinase B (AKT) and activated fragments of caspase-9 and caspase-3 were examined by Western blotting analysis. RESULTS: WWOX significantly inhibited cell proliferation, as evaluated by the MTT and colony formation assays. Cells transfected with WWOX showed significantly higher apoptosis ratios when compared with cells transfected with a mock plasmid, and overexpression of WWOX delayed cell cycle progression from G1 to S phase, as measured by flow cytometry. An increase in apoptosis was also indicated by a remarkable activation of caspase-9 and caspase-3 and a dephosphorylation of AKT (Thr308 and Ser473) measured with Western blotting analysis. CONCLUSION: Overexpression of WWOX induces apoptosis and inhibits proliferation of the human hepatic carcinoma cell line SMMC-7721.展开更多
Background Reserpine is currently used by millions of Chinese hypertensive patients, in spite of the continued concern of its depressogenic effect, even when used in low dose. This study aimed to investigate the assoc...Background Reserpine is currently used by millions of Chinese hypertensive patients, in spite of the continued concern of its depressogenic effect, even when used in low dose. This study aimed to investigate the association between low-dose reserpine use and depression in older Chinese hypertensive patient. Methods In this cross-sectional, case-control study, we recruited patient aged 60 years or over who had regularly taken one or two tables of “compound reserpine and triamterene tablets (CRTTs)” for more than one year (reserpine user) from 26 community health centers located in 10 provinces in China. For each patient who took CRTTs, we selected an age (within five years) and sex matched hypertensive patient who had never taken any drugs containing reserpine (non-reserpine user) as control. Depressive symptoms were evaluated using a Chinese depression scale adapted from the Zung Self-Rating Depression Scale. Demographic, clinical data and laboratory examination results within six months were collected. Results From August 2018 to December 2018, 787 reserpine user and 787 non-reserpine user were recruited. The mean age of all study subjects was 70.3 years, with about equal numbers of males and females. The mean depression score was 40.4 in reserpine users and 40.6 in non-reserpine users (P = 0.7). The majority of study subject had a depression score < 53 (87.6% in reserpine users and 88.2% in non-reserpine users, respectively). There were no significant differences in the prevalence of mild, moderate or severe depression in reserpine users and non-reserpine users. Conclusions There is no association between low-dose reserpine use and depression in older hypertensive patient. The role of reserpine in the treatment and control of hypertension should be reconsidered;and further studies, especially randomized, controlled clinical trials to compare efficacy and safety of reserpine and other widely recommended anti-hypertensive agents are needed.展开更多
OBJECTIVE To investigate the effects of Tongmai Yangxin Pill(TMYXP)combined with metoprolol tartrate or metoprolol alone for the treatment of premature ventricular complex(PVC)in patients with symptomatic frequent PVC...OBJECTIVE To investigate the effects of Tongmai Yangxin Pill(TMYXP)combined with metoprolol tartrate or metoprolol alone for the treatment of premature ventricular complex(PVC)in patients with symptomatic frequent PVC.METHODS A total of 584 patients with symptomatic frequent PVC were randomly assigned(in a 1:1 ratio)into two groups:study group[n=292,TMYXP(40 pills twice/day,orally)combined with metoprolol tartrate(25 mg twice/day,orally)]and control group[n=292,metoprolol tartrate(25 mg twice/day,orally)plus placebo pill(40 pills twice/day,orally)].The total treatment period was eight weeks.RESULTS After eight weeks of treatment,the total effective rate of reduction of PVC in the study group and the control group were 76.4%and 51.4%,respectively(P<0.001).TMYXP combined with metoprolol tartrate demonstrated a significantly greater reduction of the frequency of PVCs compared with the metoprolol tartrate alone(−4537 times/24 h vs.−3013 times/24 h,P<0.001).The study group also showed a better result compared with the control group with respect to PVC related symptoms.In terms of New York Heart Association classification improvement,the total effective rates were 21.9%in the study group and 12.4%in the control group(P<0.05).Both the study group and the control group exhibited improvements in echocardiographic indexes.Left ventricular ejection fraction was significantly improved in the study group compared with the control group(P<0.05).There was no significant difference in the incidence of adverse events between the two groups.CONCLUSIONS Compared with metoprolol tartrate alone,TMYXP combined with metoprolol tartrate could more effectively reduce the frequency of PVC and alleviated PVC related symptoms,and improve cardiac function in patients with symptomatic PVC.展开更多
Background Elevated serum phosphorus levels may be associated with adverse outcomes in cardiovascular disease. This study aimed to investigate the relation between serum phosphorus levels and risk of all-cause mortali...Background Elevated serum phosphorus levels may be associated with adverse outcomes in cardiovascular disease. This study aimed to investigate the relation between serum phosphorus levels and risk of all-cause mortality in Chinese patients with ST-segment elevation myocardial infarction (STEMI) who had preserved renal function at baseline. Methods We enrolled patients with STEMI who had preserved renal function at baseline in Xuanwu Hospital from January 2011 to December 2016. Those patients were divided into four groups based on serum phosphorus levels. All-cause mortality rates were compared between groups. Mean duration of follow up was 54.6 months. We used Cox proportional-hazards models to examine the relation between serum phosphorus levels and all-cause mortality after adjustment for potential confounders. Results 1989 patients were involved and 211 patients (10.6%) died during follow-up. Based on serum phosphorus levels, patients were categorized into the following groups:< 2.50 mg/dL (n = 89), 2.51–3.50 mg/dL (n = 1066), 3.51–4.50 mg/dL (n = 672) and > 4.50 mg/dL (n = 162), respectively. The lowest mortality occurred in patients with serum phosphorus levels between 2.51–3.50 mg/dL, with a multivariable-adjusted hazard ratio of 1.19 (95% CI: 0.64–1.54), 1.37 (95% CI: 1.22–1.74), and 1.46 (95% CI: 1.35–1.83) in patients with serum phosphorus levels of < 2.50 mg/dL, 3.51–4.50 mg/dL and > 4.50 mg/dL, respectively. Conclusions Elevated serum phosphorus levels were associated with all-cause mortality in Chinese patients with STEMI who had preserved renal function at baseline.展开更多
Background Endothelial dysfunction is the initial stage in atherosclerotic formation and progression and is associated with high serum uric acid(SUA)level.We hypothesized that reactive hyperemia index(RHI),which refle...Background Endothelial dysfunction is the initial stage in atherosclerotic formation and progression and is associated with high serum uric acid(SUA)level.We hypothesized that reactive hyperemia index(RHI),which reflects endothelial function,is associated with SUA levels in elderly individuals with untreated mild hypertension.Methods We recruited 123 patients≥60 years with untreated mild hypertension.The association between SUA level and RHI was analyzed using univariate correlation analysis and multiple regression analysis.The receiver operating characteristic(ROC)curve was performed to validate the cutoff value of SUA that can be used to predict endothelial dysfunction.Results The serum uric acid level significantly increased in the RHI<1.67 group,and this result was still observed in the subgroup of men.RHI was inversely associated with SUA level(P=0.006)and the association was still observed after adjusting for factors,such as age,sex,smoking status,and creatinine level(P=0.014).In the subgroup analysis,a positive association was observed only in men.In the ROC curve analysis,the optimal cutoff values of SUA for predicting endothelial dysfunction was 293.5μmol/L in elderly mild hypertension patients and 287.0μmol/L in men.Conclusion A high SUA level was considered an independent predictor of endothelial dysfunction among elderly individuals,particularly men with untreated mild hypertension.展开更多
Coarctation of the aorta(CoA)refers to the congenital coarctation near the isthmus,ductus arteriosus or ligamentum arteriosus,with an incidence of 5%–10%in children with congenital heart defects.[1]It can be isolated...Coarctation of the aorta(CoA)refers to the congenital coarctation near the isthmus,ductus arteriosus or ligamentum arteriosus,with an incidence of 5%–10%in children with congenital heart defects.[1]It can be isolated or coexisted with ventricular septal defects,subaortic stenosis,patent ductus arteriosus,and mitral aortic valve.[2]CoA has a poor prognosis.Without intervention,the median age of death with aortic coarctation is 38 years[3]and 75%death of those patients is at the age of 46 years.[4]Causes of death included congestive heart failure(26%),aortic rupture(21%),bacterial endocarditis(18%),and intracranial hemorrhage(12%).[5]Therefore,early diagnosis and optimal treatment are the key to improve the prognosis of this disease.The aim of this report is underlining the importance of early and accurate diagnose of CoA as a cause of systemic hypertension in young patients and also emphasizing the genetic factors of CoA in twins.展开更多
文摘AIM: To investigate the effects of the WWOX gene on the human hepatic carcinoma cell line SMMC-7721. METHODS: Full-length WWOX cDNA was amplified from normal human liver tissues. Full-length cDNA was subcloned into pEGFP-N1, a eukaryotic expression vector. After introduction of the WWOX gene into cancer cells using liposomes, the WWOX protein level in the cells was detected through Western blotting. Cell growth rates were assessed by methyl thiazolyl tetrazolium (MTT) and colony formation assays. Cell cycle progression and cell apoptosis were measured by flow cytometry. The phosphorylated protein kinase B (AKT) and activated fragments of caspase-9 and caspase-3 were examined by Western blotting analysis. RESULTS: WWOX significantly inhibited cell proliferation, as evaluated by the MTT and colony formation assays. Cells transfected with WWOX showed significantly higher apoptosis ratios when compared with cells transfected with a mock plasmid, and overexpression of WWOX delayed cell cycle progression from G1 to S phase, as measured by flow cytometry. An increase in apoptosis was also indicated by a remarkable activation of caspase-9 and caspase-3 and a dephosphorylation of AKT (Thr308 and Ser473) measured with Western blotting analysis. CONCLUSION: Overexpression of WWOX induces apoptosis and inhibits proliferation of the human hepatic carcinoma cell line SMMC-7721.
文摘Background Reserpine is currently used by millions of Chinese hypertensive patients, in spite of the continued concern of its depressogenic effect, even when used in low dose. This study aimed to investigate the association between low-dose reserpine use and depression in older Chinese hypertensive patient. Methods In this cross-sectional, case-control study, we recruited patient aged 60 years or over who had regularly taken one or two tables of “compound reserpine and triamterene tablets (CRTTs)” for more than one year (reserpine user) from 26 community health centers located in 10 provinces in China. For each patient who took CRTTs, we selected an age (within five years) and sex matched hypertensive patient who had never taken any drugs containing reserpine (non-reserpine user) as control. Depressive symptoms were evaluated using a Chinese depression scale adapted from the Zung Self-Rating Depression Scale. Demographic, clinical data and laboratory examination results within six months were collected. Results From August 2018 to December 2018, 787 reserpine user and 787 non-reserpine user were recruited. The mean age of all study subjects was 70.3 years, with about equal numbers of males and females. The mean depression score was 40.4 in reserpine users and 40.6 in non-reserpine users (P = 0.7). The majority of study subject had a depression score < 53 (87.6% in reserpine users and 88.2% in non-reserpine users, respectively). There were no significant differences in the prevalence of mild, moderate or severe depression in reserpine users and non-reserpine users. Conclusions There is no association between low-dose reserpine use and depression in older hypertensive patient. The role of reserpine in the treatment and control of hypertension should be reconsidered;and further studies, especially randomized, controlled clinical trials to compare efficacy and safety of reserpine and other widely recommended anti-hypertensive agents are needed.
基金supported by the Tianjin Lerentang Pharmaceutical Factory
文摘OBJECTIVE To investigate the effects of Tongmai Yangxin Pill(TMYXP)combined with metoprolol tartrate or metoprolol alone for the treatment of premature ventricular complex(PVC)in patients with symptomatic frequent PVC.METHODS A total of 584 patients with symptomatic frequent PVC were randomly assigned(in a 1:1 ratio)into two groups:study group[n=292,TMYXP(40 pills twice/day,orally)combined with metoprolol tartrate(25 mg twice/day,orally)]and control group[n=292,metoprolol tartrate(25 mg twice/day,orally)plus placebo pill(40 pills twice/day,orally)].The total treatment period was eight weeks.RESULTS After eight weeks of treatment,the total effective rate of reduction of PVC in the study group and the control group were 76.4%and 51.4%,respectively(P<0.001).TMYXP combined with metoprolol tartrate demonstrated a significantly greater reduction of the frequency of PVCs compared with the metoprolol tartrate alone(−4537 times/24 h vs.−3013 times/24 h,P<0.001).The study group also showed a better result compared with the control group with respect to PVC related symptoms.In terms of New York Heart Association classification improvement,the total effective rates were 21.9%in the study group and 12.4%in the control group(P<0.05).Both the study group and the control group exhibited improvements in echocardiographic indexes.Left ventricular ejection fraction was significantly improved in the study group compared with the control group(P<0.05).There was no significant difference in the incidence of adverse events between the two groups.CONCLUSIONS Compared with metoprolol tartrate alone,TMYXP combined with metoprolol tartrate could more effectively reduce the frequency of PVC and alleviated PVC related symptoms,and improve cardiac function in patients with symptomatic PVC.
文摘Background Elevated serum phosphorus levels may be associated with adverse outcomes in cardiovascular disease. This study aimed to investigate the relation between serum phosphorus levels and risk of all-cause mortality in Chinese patients with ST-segment elevation myocardial infarction (STEMI) who had preserved renal function at baseline. Methods We enrolled patients with STEMI who had preserved renal function at baseline in Xuanwu Hospital from January 2011 to December 2016. Those patients were divided into four groups based on serum phosphorus levels. All-cause mortality rates were compared between groups. Mean duration of follow up was 54.6 months. We used Cox proportional-hazards models to examine the relation between serum phosphorus levels and all-cause mortality after adjustment for potential confounders. Results 1989 patients were involved and 211 patients (10.6%) died during follow-up. Based on serum phosphorus levels, patients were categorized into the following groups:< 2.50 mg/dL (n = 89), 2.51–3.50 mg/dL (n = 1066), 3.51–4.50 mg/dL (n = 672) and > 4.50 mg/dL (n = 162), respectively. The lowest mortality occurred in patients with serum phosphorus levels between 2.51–3.50 mg/dL, with a multivariable-adjusted hazard ratio of 1.19 (95% CI: 0.64–1.54), 1.37 (95% CI: 1.22–1.74), and 1.46 (95% CI: 1.35–1.83) in patients with serum phosphorus levels of < 2.50 mg/dL, 3.51–4.50 mg/dL and > 4.50 mg/dL, respectively. Conclusions Elevated serum phosphorus levels were associated with all-cause mortality in Chinese patients with STEMI who had preserved renal function at baseline.
文摘Background Endothelial dysfunction is the initial stage in atherosclerotic formation and progression and is associated with high serum uric acid(SUA)level.We hypothesized that reactive hyperemia index(RHI),which reflects endothelial function,is associated with SUA levels in elderly individuals with untreated mild hypertension.Methods We recruited 123 patients≥60 years with untreated mild hypertension.The association between SUA level and RHI was analyzed using univariate correlation analysis and multiple regression analysis.The receiver operating characteristic(ROC)curve was performed to validate the cutoff value of SUA that can be used to predict endothelial dysfunction.Results The serum uric acid level significantly increased in the RHI<1.67 group,and this result was still observed in the subgroup of men.RHI was inversely associated with SUA level(P=0.006)and the association was still observed after adjusting for factors,such as age,sex,smoking status,and creatinine level(P=0.014).In the subgroup analysis,a positive association was observed only in men.In the ROC curve analysis,the optimal cutoff values of SUA for predicting endothelial dysfunction was 293.5μmol/L in elderly mild hypertension patients and 287.0μmol/L in men.Conclusion A high SUA level was considered an independent predictor of endothelial dysfunction among elderly individuals,particularly men with untreated mild hypertension.
文摘Coarctation of the aorta(CoA)refers to the congenital coarctation near the isthmus,ductus arteriosus or ligamentum arteriosus,with an incidence of 5%–10%in children with congenital heart defects.[1]It can be isolated or coexisted with ventricular septal defects,subaortic stenosis,patent ductus arteriosus,and mitral aortic valve.[2]CoA has a poor prognosis.Without intervention,the median age of death with aortic coarctation is 38 years[3]and 75%death of those patients is at the age of 46 years.[4]Causes of death included congestive heart failure(26%),aortic rupture(21%),bacterial endocarditis(18%),and intracranial hemorrhage(12%).[5]Therefore,early diagnosis and optimal treatment are the key to improve the prognosis of this disease.The aim of this report is underlining the importance of early and accurate diagnose of CoA as a cause of systemic hypertension in young patients and also emphasizing the genetic factors of CoA in twins.
