BACKGROUND The diagnostic and economic value of carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9)and CA72-4 for gastrointestinal malignant tumors lacked evaluation in a larger scale.AIM To reassess the d...BACKGROUND The diagnostic and economic value of carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9)and CA72-4 for gastrointestinal malignant tumors lacked evaluation in a larger scale.AIM To reassess the diagnostic and economic value of the three tumor biomarkers.METHODS A retrospective analysis of all 32857 subjects who underwent CEA,CA19-9,CA72-4,gastroscopy and colonoscopy from October 2006 to May 2018 was conducted.Then,we assessed the discrimination and clinical usefulness.Total cost,cost per capita and cost-effectiveness ratios were used to evaluate the economic value of two schemes(gastrointestinal endoscopy for all people without blood tests vs both gastroscopy and colonoscopy when blood tests were positive).RESULTS The analysis of 32857 subjects showed that CEA was a qualified biomarker for colorectal cancer(CRC),while the diagnostic efficiencies of CA72-4 were catastrophic for all gastrointestinal cancers(GICs).Regarding early diagnosis,only CEA could be used for early CRC.The combination of biomarkers didn’t greatly increase the area under the curve.The economic indicators of CEA were superior to those of CA19-9,CA72-4 and any combination.At the threshold of 1.8μg/L to 10.4μg/L,all four indicators of CEA were lower than those in the scheme that conducted gastrointestinal endoscopy only.Subgroup analysis implied that the health checkup of CEA for people above 65 years old was economically valuable.CONCLUSION CEA had qualified diagnostic value for CRC and superior economic value for GICs,especially for elderly health checkup subjects.CA72-4 was not suitable as a diagnostic biomarker.展开更多
Hysteresis loops,energy products and magnetic moment distributions of perpendicularly oriented Nd2Fe(14)B/α-Fe exchange-spring multilayers are studied systematically based on both three-dimensional(3D)and one-dimensi...Hysteresis loops,energy products and magnetic moment distributions of perpendicularly oriented Nd2Fe(14)B/α-Fe exchange-spring multilayers are studied systematically based on both three-dimensional(3D)and one-dimensional(1D)micromagnetic methods,focused on the influence of the interface anisotropy.The calculated results are carefully compared with each other.The interface anisotropy effect is very palpable on the nucleation,pinning and coercive fields when the soft layer is very thin.However,as the soft layer thickness increases,the pinning and coercive fields are almost unchanged with the increment of interface anisotropy though the nucleation field still monotonically rises.Negative interface anisotropy decreases the maximum energy products and increases slightly the angles between the magnetization and applied field.The magnetic moment distributions in the thickness direction at various applied fields demonstrate a progress of three-step magnetic reversal,i.e.,nucleation,evolution and irreversible motion of the domain wall.The above results calculated by two models are in good agreement with each other.Moreover,the in-plane magnetic moment orientations based on two models are different.The 3D calculation shows a progress of generation and disappearance of vortex state,however,the magnetization orientations within the film plane calculated by the 1D model are coherent.Simulation results suggest that negative interface anisotropy is necessarily avoided experimentally.展开更多
Background:To explore the relevant targets of the Chinese herbal medicine compound Danggui-Sini Decoction(DGSND)for the treatment of systemic sclerosis(SSc).Method:We used TCMSP to enlist ingredients and Swiss Target ...Background:To explore the relevant targets of the Chinese herbal medicine compound Danggui-Sini Decoction(DGSND)for the treatment of systemic sclerosis(SSc).Method:We used TCMSP to enlist ingredients and Swiss Target Prediction for targets fishing,and the protein names by UniProt for organized as gene symbol.Strawberry Perl was used to integrate the active ingredients and the drugs action target of DGSND,with Cytoscape 3.9.0 software to construct"Active ingredient-Drug target"network.Then,GEO database,GeneCards database,TTDdatabase,DisGENent database and MalaCards database for SSc-related disease target prediction,and then analyzed the active drug targets of DGSND and SSc-related targets of Danggui-Sini Decoction treat SSc.Then,the above results we combined with STRING database to visualize the Protein-protein interaction(PPI)network for the core targets of SSc,and performed Gene ontology(GO)functional analysis and Kyoto Encyclopedia of Genomics(KEGG)signaling pathway enrichment analysis for SSc-related targets treated with DGSND Result:DGSND contains 223 active ingredients including Sitogluside,Benzoylpaeoniflorin,(-)-Asarinin Palbinone,Glycyro,4'DMEP etc.which acts on Signal transducer and activator of transcription 3(STAT3),Tumor Necrosis Factor(TNF),Vascular endothelial growth factor(VEGFA),Nuclear factor kappa-B(NFκB1),Interleukin(IL1β,IL17),Mitogen-activated protein kinase(MAPK)and Janus Kinase(JAK)and many other genes totaling 176,mainly involved in 4 more biological processes 3 molecular functions and 3 cellular components.Conclusion:DGSND is primarily used to treat SSc by regulating calcium homeostasis,inflammatory signaling pathways and neural cell repair and apoptosis-related pathways within the body.展开更多
DEAR EDITOR,The COVID-19 pandemic caused by SARS-CoV-2 continues to pose a tremendous threat to human society. SARS-CoV-2is airborne and transmits primarily through social contact;however, whether cold chain-related t...DEAR EDITOR,The COVID-19 pandemic caused by SARS-CoV-2 continues to pose a tremendous threat to human society. SARS-CoV-2is airborne and transmits primarily through social contact;however, whether cold chain-related transmission has occurred remains highly debated(Han & Liu, 2022;Lewis,2021;Ma et al., 2021;Mallapaty et al., 2021;Pang et al.,2020;Wu et al., 2021). Here, we present a novel method and identify two transmission routes based on lineage-specific reductions in the SARS-CoV-2 evolutionary rate.展开更多
Chinese medicine.However,little is known about the potential mechanism.Elucidating the effective components and mechanism based on network pharmacology was our purpose.Methods:Traditional Chinese Medicine Systems Phar...Chinese medicine.However,little is known about the potential mechanism.Elucidating the effective components and mechanism based on network pharmacology was our purpose.Methods:Traditional Chinese Medicine Systems Pharmacology was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem,and the related potential targets were further predicted in Swiss Target Prediction database.Coronary heart disease molecular members were gained from GeenCards database,and the predicted targets of Cinnamomi Ramulus for coronary heart disease’s treatment were selected by Wayne diagram.As for mechanism analysis,String was used to construct the protein-protein interactions,and DAVID was used to conduct the GO and KEGG analysis.Results:Through GO and KEGG analysis,we found that cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway was related with coronary heart disease.Using network-based systems biology,we predicted that 10 active ingredients in Cinnamomi Ramulus have the treating effects with 78 potential targets.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were mainly involved in the treating effects of Cinnamomi Ramulus.Conclusion:Cinnamomi Ramulus may treat coronary heart disease through cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were supposed to be considerable targets for treating coronary heart disease.展开更多
Depression is a kind of mental disorder characterized by conspicuous and persistent low mood, sustained sadness, loss of interest and forgetfulness. In traditional Chinese medicine, heart (different from the concept i...Depression is a kind of mental disorder characterized by conspicuous and persistent low mood, sustained sadness, loss of interest and forgetfulness. In traditional Chinese medicine, heart (different from the concept in Western medicine) control the emotions. Overthinking hinders the function of the spleen. Deficiency of spleen leads to lack of heart-Qi. Deficiency of the spleen also leads to dysfunction of heart. Once the heart is dysfunction, Qi will be stagnated in Danzhong(thoracic center), eventually leads to depression. In Traditional Chinese medicine, the development of depression is a heart-spleen-heart process, which can be called the "heart-spleen axis". Biomedicine studies have found that the progress of depression is mainly caused by long-term adverse stimulation of the central nervous system, which affects the Hypothalamic-pituitary-adrenal axis (HPA) to convey monoamine neurotransmitters, such as 5- hydroxytryptamine (5-HT), and further damaged the intestinal mucosal barrier, increased its permeability, and produced a large number of lipopolysaccharides (LPS) and inflammatory factors. The production of LPS and inflammatory cytokines reduces the synthesis of 5-HT by consuming tryptophan, and the decrease of 5-HT in serum results in the decrease of 5-HT levels in the brain. At the same time, changes in the intestinal microbiota alter the concentration of brain-derived neurotrophic factor (BDNF) via the vagal pathway, ultimately creating a vicious brain-gut- brain cycle. Kaixin Powder has the effect of invigorating spleen and eliminating dampness, awaking consciousness and inducing resuscitation, and can adjust the imbalance of gut microbiota and neurotransmitters to achieve the purpose of treating depression.展开更多
Background:This study aimed to deeply explore the active components of Danggui Buxue Decoction(DBD)through systematic network pharmacology and molecular docking methods and to demonstrate its mechanism of regulating T...Background:This study aimed to deeply explore the active components of Danggui Buxue Decoction(DBD)through systematic network pharmacology and molecular docking methods and to demonstrate its mechanism of regulating Thalassemia.Methods:Obtain and screen the active ingredients and targets of DBD from the TCMSP database.Use the GeneCards database to search for the gene corresponding to the target.The target protein-protein interaction(PPI)network was built using STRING database.A DBD-drug-Tha-target PPI network was established,and its core target network was analyzed using Cytoscape software.Then,enrichment analysis of DBD core targets was performed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and their binding activities were verified by molecular docking.Results:Finally,19 active ingredients and 504 targets were obtained from DBD,of which 98 targets were repeated with the related targets of Tha.The major genes include HSP90AA1,IL-6 and HRAS.Through the final analysis,we found that the main mechanisms of DBD in the treatment of thalassemia include increasing the expression of the γ-globin gene in red blood cells,inducing fetal hemoglobin and reducing the inflammatory response.Conclusion:The potential mechanism of DBD improving thalassemia can be preliminarily predicted by network pharmacology and molecular docking,which can provide some reference for clinical treatment.展开更多
Water and liquid are the material basis of human metabolism.Traditional Chinese medicine believes that water and liquid metabolism is a very complex process that requires the synergy of the internal organs,but mainly ...Water and liquid are the material basis of human metabolism.Traditional Chinese medicine believes that water and liquid metabolism is a very complex process that requires the synergy of the internal organs,but mainly the physiological functions of the lungs,spleen and kidneys.If the function or structure of the spleen,lungs,kidneys,or triple-burner is abnormal,it is easy to cause abnormalities in the body's water metabolism,which in turn leads to the production of pathological products such as damp phlegm.展开更多
Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese ...Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese medicine.There are many names of pulse conditions in traditional Chinese medicine.The four elements are analyzed and summarized in terms of position,number,shape,and momentum.The four elements are used as 28 kinds of pulses.Among them,the regularly intermittent pulse and the intermittent pulse are characterized by arrhythmia and intermittent stop of the pulse.Intermittent pulse means irregular intervals;when a pulse comes,Regularly Intermittent pulse have regular stops.This kind of phenomenon is similar to the bradyarrhythmia in modern medicine.The knot pulse is an irregular pulse stopping,which can be seen clinically in sinus arrest,second-degree type I sinus block,second-degree type I atrioventricular block,and escape beats and so on.On the other hand,Regularly Intermittent pulse is a regular pulse stopping,which can be clinically found in the second degree type II sinus block,the second degree type II sinus block,and the second degree type I atrioventricular block with a special 2:1 type of atrioventricular block type and so on.展开更多
Objective To observe the influence of Kaixin Powder on ethology,content of 5-HT in the hippocampus, expression of m RNA, and protein in 5-HT1 A and 5-HT2 A receptors in the hippocampus of depressed rats induced by chr...Objective To observe the influence of Kaixin Powder on ethology,content of 5-HT in the hippocampus, expression of m RNA, and protein in 5-HT1 A and 5-HT2 A receptors in the hippocampus of depressed rats induced by chronic unpredictable mild stress(CUMS). Methods Twenty-four male Wistar rats were randomly divided into blank,model, Trazodone, and Kaixin Powder groups, six rats in each group. In addition to the blank control group, other groups were established the depression model induced by CUMS combined with isolated feeding. At the same time, Trazodone group and Kaixin Powder group were treated with corresponding drugs for 3 weeks. After 3 weeks of administration, the rats were sacrificed, and a series of indexes were measured such as the contents of 5-HT, m RNA expression levels of 5-HT1 A and 5-HT2 A receptors, protein expression levels of 5-HT1 A and 5-HT2 A receptors, and so on. Results A series of indexes in the model group were decreased significantly such as the body weight growth, the sugar water intake, the score of Open Field Test, the content of5-HT in the hippocampus, expression of m RNA, and protein in 5-HT1 A receptor, while the expression of m RNA and protein in 5-HT2 A receptor were increased significantly.Compared with the model group, the indexes were ameliorated in Trazodone and Kaixin Powder groups. Kaixin Powder is better than Trazodone in decreasing the level of protein in 5-HT2 A receptor. Conclusion The result indicated that the depression performance of depressed rats induced by CUMS can be ameliorated by Kaixin Powder,and the mechanism maybe concerned with increasing the contents of 5-HT, exciting 5-HT1 A receptor, and antagonising 5- HT2 A receptor.展开更多
Two models are established to reveal the underlying coercivity mechanism for SmCo/Fe films,where one model considers a transition layer between hard and soft layers,while the other model does not consider this layer.B...Two models are established to reveal the underlying coercivity mechanism for SmCo/Fe films,where one model considers a transition layer between hard and soft layers,while the other model does not consider this layer.Based on the two models,hysteresis loops,nucleation fields and coercivity are obtained by one-dimensional(1 D)and three-dimensional(3 D)micromagnetic methods.In particular,the calculated nucleation fields(H_N)and coercivity(H_C)match very well with the experimental data.It is found that the increase in the soft phase thickness(Ls)leads to a transition of the coercivity mechanism from nucleation to pinning.Such a pinning is inherently related to nucleation and has both attributes of traditional nucleation and pinning,called as a hybrid coercivity mechanism here.It is general for all hard/soft composites and can be extended to single-phased permanent magnets where defects are inevitable.展开更多
Skyrmions are nano-scale quasi-particles with topological protection,which have potential applications in next-generation spintronics-based information storage.Numerous papers have been published to review various asp...Skyrmions are nano-scale quasi-particles with topological protection,which have potential applications in next-generation spintronics-based information storage.Numerous papers have been published to review various aspects of skyrmions,including physics,materials and applications.However,no review paper has focused on rare metals which play important roles in nucleating and manipulating skyrmions and other topological states.In this paper,various roles of rare metals have been classified and summarized,which can tune Curie temperature(TC),Dzyaloshinskii-Moriya interaction(DMI),magnetocrystalline anisotropy,Ruderman-Kittel-Kasuya-Yosida(RKKY)interaction and four-spin interaction so as to trigger the generation of skyrmions and other topological spin structures.The materials covered include typical B20 crystals,various layered systems with interfacial DMI,frustrated materials,antiferromagnets,ferrimagnets,twodimensional(2D)materials,etc.In addition,the rare-earth(RE)permanent magnets can provide an energy barrier and enrich the dynamic behaviors of skyrmions,which has also been reviewed.展开更多
The complete genome of methanol-utilizing Amycolatopsis methanolica strain 239T was generated,revealing a single 7,237,391 nucleotide circular chromosome with 7074 annotated protein-coding sequences(CDSs).Comparative ...The complete genome of methanol-utilizing Amycolatopsis methanolica strain 239T was generated,revealing a single 7,237,391 nucleotide circular chromosome with 7074 annotated protein-coding sequences(CDSs).Comparative analyses against the complete genome sequences of Amycolatopsis japonica strain MG417-CF17T,Amycolatopsis mediterranei strain U32 and Amycolatopsis orientalis strain HCCB10007 revealed a broad spectrum of genomic structures,including various genome sizes,core/quasi-core/non-core configurations and different kinds of episomes.Although polyketide synthase gene clusters were absent from the A.methanolica genome,12 gene clusters related to the biosynthesis of other specialized(secondary)metabolites were identified.Complete pathways attributable to the facultative methylotrophic physiology of A.methanolica strain 239T,including both the mdo/mscR encoded methanol oxidation and the hps/hpi encoded formaldehyde assimilation via the ribulose monophosphate cycle,were identified together with evidence that the latter might be the result of horizontal gene transfer.Phylogenetic analyses based on 16S rDNA or orthologues of AMETH_3452,a novel actinobacterial class-specific conserved gene against 62 or 18 Amycolatopsis type strains,respectively,revealed three major phyletic lineages,namely the mesophilic or moderately thermophilic A.orientalis subclade(AOS),the mesophilic Amycolatopsis taiwanensis subclade(ATS)and the thermophilic A.methanolica subclade(AMS).The distinct growth temperatures of members of the subclades correlated with corresponding genetic variations in their encoded compatible solutes.This study shows the value of integrating conventional taxonomic with whole genome sequence data.展开更多
CaCu3Ti4O12 ceramics doped with different contents of Sc2O3(mol%, x = 0, 0.05, 0.10, 0.15, and 0.20) were prepared by the traditional solid-state reaction method. Scanning electron microscope(SEM) and X-ray diffractio...CaCu3Ti4O12 ceramics doped with different contents of Sc2O3(mol%, x = 0, 0.05, 0.10, 0.15, and 0.20) were prepared by the traditional solid-state reaction method. Scanning electron microscope(SEM) and X-ray diffraction(XRD) were used in the microstructural studies of the specimen, and the electrical properties were investigated. XRD results show that the Sc has no influence on the phase composition. The results from the dielectric measurements show that further increase of Sc doping could decrease the dielectric loss slightly. A high dielectric constant and low dielectric loss can be achieved when the doping concentration is 0.10 mol%.展开更多
Background:The ongoing transmission of the Middle East respiratory syndrome coronavirus(MERS-CoV)in the Middle East and its expansion to other regions are raising concerns of a potential pandemic.An in-depth analysis ...Background:The ongoing transmission of the Middle East respiratory syndrome coronavirus(MERS-CoV)in the Middle East and its expansion to other regions are raising concerns of a potential pandemic.An in-depth analysis about both population and molecular epidemiology of this pathogen is needed.Methods:MERS cases reported globally as of June 2020 were collected mainly from World Health Organization official reports,supplemented by other reliable sources.Determinants for case fatality and spatial diffusion of MERS were assessed with Logistic regressions and Cox proportional hazard models,respectively.Phylogenetic and phylogeographic analyses were performed to examine the evolution and migration history of MERS-CoV.Results:A total of 2562 confirmed MERS cases with 150 case clusters were reported with a case fatality rate of 32.7%(95%Cl:30.9-34.6%).Saudi Arabia accounted for 83.6%of the cases.Age of>65 years old,underlying conditions and>5 days delay in diagnosis were independent risk factors for death.However,a history of animal contact was associated with a higher risk(adjusted OR=297,95%Cl:1」0-7.98)among female cases<65 years but with a lower risk(adjusted OR=0.31,95%Cl:0.18-0.51)among male cases>65 years old.Diffusion of the disease was fastest from its origin in Saudi Arabia to the east,and was primarily driven by the transportation network.The most recent subclade C5.1(since 2013)was associated with non-synonymous mutations and a higher mortality rate.Phylogeographic analyses pointed to Riyadh of Saudi Arabia and Abu Dhabi of the United Arab Emirates as the hubs for both local and international spread of MERS-CoV.Conclusions:MERS-CoV remains primarily locally transmitted in the Middle East,with opportunistic exportation to other continents and a potential of causing transmission clusters of human cases.Animal contact is associated with a higher risk of death,but the association differs by age and sex.Transportation network is the leading driver for the spatial diffusion ofthe disease.These findings how this pathogen spread are helpful for targeting public health surveillance and interventions to control endemics and to prevent a potential pandemic.展开更多
基金The study was reviewed and approved by the Zhongshan Hospital of Fudan University Institutional Review Board(Approval No.B2018-234).
文摘BACKGROUND The diagnostic and economic value of carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9)and CA72-4 for gastrointestinal malignant tumors lacked evaluation in a larger scale.AIM To reassess the diagnostic and economic value of the three tumor biomarkers.METHODS A retrospective analysis of all 32857 subjects who underwent CEA,CA19-9,CA72-4,gastroscopy and colonoscopy from October 2006 to May 2018 was conducted.Then,we assessed the discrimination and clinical usefulness.Total cost,cost per capita and cost-effectiveness ratios were used to evaluate the economic value of two schemes(gastrointestinal endoscopy for all people without blood tests vs both gastroscopy and colonoscopy when blood tests were positive).RESULTS The analysis of 32857 subjects showed that CEA was a qualified biomarker for colorectal cancer(CRC),while the diagnostic efficiencies of CA72-4 were catastrophic for all gastrointestinal cancers(GICs).Regarding early diagnosis,only CEA could be used for early CRC.The combination of biomarkers didn’t greatly increase the area under the curve.The economic indicators of CEA were superior to those of CA19-9,CA72-4 and any combination.At the threshold of 1.8μg/L to 10.4μg/L,all four indicators of CEA were lower than those in the scheme that conducted gastrointestinal endoscopy only.Subgroup analysis implied that the health checkup of CEA for people above 65 years old was economically valuable.CONCLUSION CEA had qualified diagnostic value for CRC and superior economic value for GICs,especially for elderly health checkup subjects.CA72-4 was not suitable as a diagnostic biomarker.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFB0700900)the National Natural Science Foundation of China(Grant Nos.51571126 and 51861030)+1 种基金the Inner Mongolia Autonomous Region Natural Science Foundation of China(Grant No.2019MS01002)the Inner Mongolia Innovative Research Team of China(Grant No.3400102)。
文摘Hysteresis loops,energy products and magnetic moment distributions of perpendicularly oriented Nd2Fe(14)B/α-Fe exchange-spring multilayers are studied systematically based on both three-dimensional(3D)and one-dimensional(1D)micromagnetic methods,focused on the influence of the interface anisotropy.The calculated results are carefully compared with each other.The interface anisotropy effect is very palpable on the nucleation,pinning and coercive fields when the soft layer is very thin.However,as the soft layer thickness increases,the pinning and coercive fields are almost unchanged with the increment of interface anisotropy though the nucleation field still monotonically rises.Negative interface anisotropy decreases the maximum energy products and increases slightly the angles between the magnetization and applied field.The magnetic moment distributions in the thickness direction at various applied fields demonstrate a progress of three-step magnetic reversal,i.e.,nucleation,evolution and irreversible motion of the domain wall.The above results calculated by two models are in good agreement with each other.Moreover,the in-plane magnetic moment orientations based on two models are different.The 3D calculation shows a progress of generation and disappearance of vortex state,however,the magnetization orientations within the film plane calculated by the 1D model are coherent.Simulation results suggest that negative interface anisotropy is necessarily avoided experimentally.
文摘Background:To explore the relevant targets of the Chinese herbal medicine compound Danggui-Sini Decoction(DGSND)for the treatment of systemic sclerosis(SSc).Method:We used TCMSP to enlist ingredients and Swiss Target Prediction for targets fishing,and the protein names by UniProt for organized as gene symbol.Strawberry Perl was used to integrate the active ingredients and the drugs action target of DGSND,with Cytoscape 3.9.0 software to construct"Active ingredient-Drug target"network.Then,GEO database,GeneCards database,TTDdatabase,DisGENent database and MalaCards database for SSc-related disease target prediction,and then analyzed the active drug targets of DGSND and SSc-related targets of Danggui-Sini Decoction treat SSc.Then,the above results we combined with STRING database to visualize the Protein-protein interaction(PPI)network for the core targets of SSc,and performed Gene ontology(GO)functional analysis and Kyoto Encyclopedia of Genomics(KEGG)signaling pathway enrichment analysis for SSc-related targets treated with DGSND Result:DGSND contains 223 active ingredients including Sitogluside,Benzoylpaeoniflorin,(-)-Asarinin Palbinone,Glycyro,4'DMEP etc.which acts on Signal transducer and activator of transcription 3(STAT3),Tumor Necrosis Factor(TNF),Vascular endothelial growth factor(VEGFA),Nuclear factor kappa-B(NFκB1),Interleukin(IL1β,IL17),Mitogen-activated protein kinase(MAPK)and Janus Kinase(JAK)and many other genes totaling 176,mainly involved in 4 more biological processes 3 molecular functions and 3 cellular components.Conclusion:DGSND is primarily used to treat SSc by regulating calcium homeostasis,inflammatory signaling pathways and neural cell repair and apoptosis-related pathways within the body.
基金supported by the National Key Research and Development Project (2020YFC0847000,2021YFC0863300, 2020YFC0845900)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDPB17)+1 种基金the National Natural Science Foundation of China (31100273, 91731304,31172073)Shandong Academician Workstation Program#170401 (to G.P.Z.)。
文摘DEAR EDITOR,The COVID-19 pandemic caused by SARS-CoV-2 continues to pose a tremendous threat to human society. SARS-CoV-2is airborne and transmits primarily through social contact;however, whether cold chain-related transmission has occurred remains highly debated(Han & Liu, 2022;Lewis,2021;Ma et al., 2021;Mallapaty et al., 2021;Pang et al.,2020;Wu et al., 2021). Here, we present a novel method and identify two transmission routes based on lineage-specific reductions in the SARS-CoV-2 evolutionary rate.
基金the National Natural Science Foundation of China(No.81874404)。
文摘Chinese medicine.However,little is known about the potential mechanism.Elucidating the effective components and mechanism based on network pharmacology was our purpose.Methods:Traditional Chinese Medicine Systems Pharmacology was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem,and the related potential targets were further predicted in Swiss Target Prediction database.Coronary heart disease molecular members were gained from GeenCards database,and the predicted targets of Cinnamomi Ramulus for coronary heart disease’s treatment were selected by Wayne diagram.As for mechanism analysis,String was used to construct the protein-protein interactions,and DAVID was used to conduct the GO and KEGG analysis.Results:Through GO and KEGG analysis,we found that cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway was related with coronary heart disease.Using network-based systems biology,we predicted that 10 active ingredients in Cinnamomi Ramulus have the treating effects with 78 potential targets.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were mainly involved in the treating effects of Cinnamomi Ramulus.Conclusion:Cinnamomi Ramulus may treat coronary heart disease through cAMP signaling pathway,estrogen signaling pathway,calcium signaling pathway.PIK3CG,MAPK8,BCL-2,BAX,PRKACA,CASP3,CALM1,CALM2,CALM3,NOS2,NOS3 were supposed to be considerable targets for treating coronary heart disease.
文摘Depression is a kind of mental disorder characterized by conspicuous and persistent low mood, sustained sadness, loss of interest and forgetfulness. In traditional Chinese medicine, heart (different from the concept in Western medicine) control the emotions. Overthinking hinders the function of the spleen. Deficiency of spleen leads to lack of heart-Qi. Deficiency of the spleen also leads to dysfunction of heart. Once the heart is dysfunction, Qi will be stagnated in Danzhong(thoracic center), eventually leads to depression. In Traditional Chinese medicine, the development of depression is a heart-spleen-heart process, which can be called the "heart-spleen axis". Biomedicine studies have found that the progress of depression is mainly caused by long-term adverse stimulation of the central nervous system, which affects the Hypothalamic-pituitary-adrenal axis (HPA) to convey monoamine neurotransmitters, such as 5- hydroxytryptamine (5-HT), and further damaged the intestinal mucosal barrier, increased its permeability, and produced a large number of lipopolysaccharides (LPS) and inflammatory factors. The production of LPS and inflammatory cytokines reduces the synthesis of 5-HT by consuming tryptophan, and the decrease of 5-HT in serum results in the decrease of 5-HT levels in the brain. At the same time, changes in the intestinal microbiota alter the concentration of brain-derived neurotrophic factor (BDNF) via the vagal pathway, ultimately creating a vicious brain-gut- brain cycle. Kaixin Powder has the effect of invigorating spleen and eliminating dampness, awaking consciousness and inducing resuscitation, and can adjust the imbalance of gut microbiota and neurotransmitters to achieve the purpose of treating depression.
基金supported by the National Natural Science Foundation of China(grant no.81874404).
文摘Background:This study aimed to deeply explore the active components of Danggui Buxue Decoction(DBD)through systematic network pharmacology and molecular docking methods and to demonstrate its mechanism of regulating Thalassemia.Methods:Obtain and screen the active ingredients and targets of DBD from the TCMSP database.Use the GeneCards database to search for the gene corresponding to the target.The target protein-protein interaction(PPI)network was built using STRING database.A DBD-drug-Tha-target PPI network was established,and its core target network was analyzed using Cytoscape software.Then,enrichment analysis of DBD core targets was performed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and their binding activities were verified by molecular docking.Results:Finally,19 active ingredients and 504 targets were obtained from DBD,of which 98 targets were repeated with the related targets of Tha.The major genes include HSP90AA1,IL-6 and HRAS.Through the final analysis,we found that the main mechanisms of DBD in the treatment of thalassemia include increasing the expression of the γ-globin gene in red blood cells,inducing fetal hemoglobin and reducing the inflammatory response.Conclusion:The potential mechanism of DBD improving thalassemia can be preliminarily predicted by network pharmacology and molecular docking,which can provide some reference for clinical treatment.
基金General program of National Natural Science Foundation of China(81874404)。
文摘Water and liquid are the material basis of human metabolism.Traditional Chinese medicine believes that water and liquid metabolism is a very complex process that requires the synergy of the internal organs,but mainly the physiological functions of the lungs,spleen and kidneys.If the function or structure of the spleen,lungs,kidneys,or triple-burner is abnormal,it is easy to cause abnormalities in the body's water metabolism,which in turn leads to the production of pathological products such as damp phlegm.
基金National Natural Science Foundation of China(81874404)。
文摘Pulse diagnosis is an important basis for the diagnosis of Chinese medicine.It is the rich experience accumulated by the predecessors in the long and continuous practice,and it is a unique diagnosis method of Chinese medicine.There are many names of pulse conditions in traditional Chinese medicine.The four elements are analyzed and summarized in terms of position,number,shape,and momentum.The four elements are used as 28 kinds of pulses.Among them,the regularly intermittent pulse and the intermittent pulse are characterized by arrhythmia and intermittent stop of the pulse.Intermittent pulse means irregular intervals;when a pulse comes,Regularly Intermittent pulse have regular stops.This kind of phenomenon is similar to the bradyarrhythmia in modern medicine.The knot pulse is an irregular pulse stopping,which can be seen clinically in sinus arrest,second-degree type I sinus block,second-degree type I atrioventricular block,and escape beats and so on.On the other hand,Regularly Intermittent pulse is a regular pulse stopping,which can be clinically found in the second degree type II sinus block,the second degree type II sinus block,and the second degree type I atrioventricular block with a special 2:1 type of atrioventricular block type and so on.
基金National Natural Science Fund of China(NO.81072744)
文摘Objective To observe the influence of Kaixin Powder on ethology,content of 5-HT in the hippocampus, expression of m RNA, and protein in 5-HT1 A and 5-HT2 A receptors in the hippocampus of depressed rats induced by chronic unpredictable mild stress(CUMS). Methods Twenty-four male Wistar rats were randomly divided into blank,model, Trazodone, and Kaixin Powder groups, six rats in each group. In addition to the blank control group, other groups were established the depression model induced by CUMS combined with isolated feeding. At the same time, Trazodone group and Kaixin Powder group were treated with corresponding drugs for 3 weeks. After 3 weeks of administration, the rats were sacrificed, and a series of indexes were measured such as the contents of 5-HT, m RNA expression levels of 5-HT1 A and 5-HT2 A receptors, protein expression levels of 5-HT1 A and 5-HT2 A receptors, and so on. Results A series of indexes in the model group were decreased significantly such as the body weight growth, the sugar water intake, the score of Open Field Test, the content of5-HT in the hippocampus, expression of m RNA, and protein in 5-HT1 A receptor, while the expression of m RNA and protein in 5-HT2 A receptor were increased significantly.Compared with the model group, the indexes were ameliorated in Trazodone and Kaixin Powder groups. Kaixin Powder is better than Trazodone in decreasing the level of protein in 5-HT2 A receptor. Conclusion The result indicated that the depression performance of depressed rats induced by CUMS can be ameliorated by Kaixin Powder,and the mechanism maybe concerned with increasing the contents of 5-HT, exciting 5-HT1 A receptor, and antagonising 5- HT2 A receptor.
基金financially supported by the National Natural Science Foundation of China(Nos.51771127,51571126 and 51772004).
文摘Two models are established to reveal the underlying coercivity mechanism for SmCo/Fe films,where one model considers a transition layer between hard and soft layers,while the other model does not consider this layer.Based on the two models,hysteresis loops,nucleation fields and coercivity are obtained by one-dimensional(1 D)and three-dimensional(3 D)micromagnetic methods.In particular,the calculated nucleation fields(H_N)and coercivity(H_C)match very well with the experimental data.It is found that the increase in the soft phase thickness(Ls)leads to a transition of the coercivity mechanism from nucleation to pinning.Such a pinning is inherently related to nucleation and has both attributes of traditional nucleation and pinning,called as a hybrid coercivity mechanism here.It is general for all hard/soft composites and can be extended to single-phased permanent magnets where defects are inevitable.
基金financially supported by the National Natural Science Foundation of China (Nos. 51771127, 52171188 52111530143, 12104327, 51901081, 11974298 and 61961136006)the National Key Research and Development Program of China (No. 2020YFA0309300)+6 种基金Sichuan Science and Technology Program (Application No. 21ZYZYTS0077)the Science and Technology Program of Guangzhou (No. 202002030052)Guangdong Special Support Project (No. 2019BT02X030)Shenzhen Fundamental Research Fund (No. JCYJ20210324120213037)Shenzhen Peacock Group Plan (No. KQTD20180413181702403)Pearl River Recruitment Program of Talents (No. 2017GC010293)the Grants-in-Aid for Scientific Research from JSPS KAKENHI (Nos. JP20F20363, JP21H01364 and JP21K18872)
文摘Skyrmions are nano-scale quasi-particles with topological protection,which have potential applications in next-generation spintronics-based information storage.Numerous papers have been published to review various aspects of skyrmions,including physics,materials and applications.However,no review paper has focused on rare metals which play important roles in nucleating and manipulating skyrmions and other topological states.In this paper,various roles of rare metals have been classified and summarized,which can tune Curie temperature(TC),Dzyaloshinskii-Moriya interaction(DMI),magnetocrystalline anisotropy,Ruderman-Kittel-Kasuya-Yosida(RKKY)interaction and four-spin interaction so as to trigger the generation of skyrmions and other topological spin structures.The materials covered include typical B20 crystals,various layered systems with interfacial DMI,frustrated materials,antiferromagnets,ferrimagnets,twodimensional(2D)materials,etc.In addition,the rare-earth(RE)permanent magnets can provide an energy barrier and enrich the dynamic behaviors of skyrmions,which has also been reviewed.
基金This work was supported in part by grants from the National Basic Research Program of China(2012CB721102,2013CB734000)the Natural Science Foundation for Youth(31300034)+1 种基金the National Natural Science Foundation of China(31270056,31430004 and 31421061)LX.Z.is an Awardee of the National Distinguished Young Scholar Program in China(31125002).
文摘The complete genome of methanol-utilizing Amycolatopsis methanolica strain 239T was generated,revealing a single 7,237,391 nucleotide circular chromosome with 7074 annotated protein-coding sequences(CDSs).Comparative analyses against the complete genome sequences of Amycolatopsis japonica strain MG417-CF17T,Amycolatopsis mediterranei strain U32 and Amycolatopsis orientalis strain HCCB10007 revealed a broad spectrum of genomic structures,including various genome sizes,core/quasi-core/non-core configurations and different kinds of episomes.Although polyketide synthase gene clusters were absent from the A.methanolica genome,12 gene clusters related to the biosynthesis of other specialized(secondary)metabolites were identified.Complete pathways attributable to the facultative methylotrophic physiology of A.methanolica strain 239T,including both the mdo/mscR encoded methanol oxidation and the hps/hpi encoded formaldehyde assimilation via the ribulose monophosphate cycle,were identified together with evidence that the latter might be the result of horizontal gene transfer.Phylogenetic analyses based on 16S rDNA or orthologues of AMETH_3452,a novel actinobacterial class-specific conserved gene against 62 or 18 Amycolatopsis type strains,respectively,revealed three major phyletic lineages,namely the mesophilic or moderately thermophilic A.orientalis subclade(AOS),the mesophilic Amycolatopsis taiwanensis subclade(ATS)and the thermophilic A.methanolica subclade(AMS).The distinct growth temperatures of members of the subclades correlated with corresponding genetic variations in their encoded compatible solutes.This study shows the value of integrating conventional taxonomic with whole genome sequence data.
基金financially supported by the National Nature Science Foundation of China(No.61205128)the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20123227120021)+3 种基金the Natural Science Foundation of Jiangsu Province(No.BK2012156)the Opening Project of State Key Laboratory of Electronic Thin Films and Integrated Devices(No.KFJJ201105)Application Program for Basic Research of Changzhou(No.CJ20125001)the Universities Natural Science Research Project of Jiangsu Province(No.13KJB430006)
文摘CaCu3Ti4O12 ceramics doped with different contents of Sc2O3(mol%, x = 0, 0.05, 0.10, 0.15, and 0.20) were prepared by the traditional solid-state reaction method. Scanning electron microscope(SEM) and X-ray diffraction(XRD) were used in the microstructural studies of the specimen, and the electrical properties were investigated. XRD results show that the Sc has no influence on the phase composition. The results from the dielectric measurements show that further increase of Sc doping could decrease the dielectric loss slightly. A high dielectric constant and low dielectric loss can be achieved when the doping concentration is 0.10 mol%.
基金supported by China Mega-Project on Infectious Disease Prevention(No.2017ZX10303401,2018ZX10713002,2018ZX10101003 and 2018ZX10201001)National Natural Science Foundation of China(No.81825019),and the National Institutes of Health of United States(R01 All 39761 and R01 AI116770)YY was supported by US National Institutes of Health grants R01 Al 139761 and R56 Al 148284.
文摘Background:The ongoing transmission of the Middle East respiratory syndrome coronavirus(MERS-CoV)in the Middle East and its expansion to other regions are raising concerns of a potential pandemic.An in-depth analysis about both population and molecular epidemiology of this pathogen is needed.Methods:MERS cases reported globally as of June 2020 were collected mainly from World Health Organization official reports,supplemented by other reliable sources.Determinants for case fatality and spatial diffusion of MERS were assessed with Logistic regressions and Cox proportional hazard models,respectively.Phylogenetic and phylogeographic analyses were performed to examine the evolution and migration history of MERS-CoV.Results:A total of 2562 confirmed MERS cases with 150 case clusters were reported with a case fatality rate of 32.7%(95%Cl:30.9-34.6%).Saudi Arabia accounted for 83.6%of the cases.Age of>65 years old,underlying conditions and>5 days delay in diagnosis were independent risk factors for death.However,a history of animal contact was associated with a higher risk(adjusted OR=297,95%Cl:1」0-7.98)among female cases<65 years but with a lower risk(adjusted OR=0.31,95%Cl:0.18-0.51)among male cases>65 years old.Diffusion of the disease was fastest from its origin in Saudi Arabia to the east,and was primarily driven by the transportation network.The most recent subclade C5.1(since 2013)was associated with non-synonymous mutations and a higher mortality rate.Phylogeographic analyses pointed to Riyadh of Saudi Arabia and Abu Dhabi of the United Arab Emirates as the hubs for both local and international spread of MERS-CoV.Conclusions:MERS-CoV remains primarily locally transmitted in the Middle East,with opportunistic exportation to other continents and a potential of causing transmission clusters of human cases.Animal contact is associated with a higher risk of death,but the association differs by age and sex.Transportation network is the leading driver for the spatial diffusion ofthe disease.These findings how this pathogen spread are helpful for targeting public health surveillance and interventions to control endemics and to prevent a potential pandemic.
