BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unkn...BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.展开更多
BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to patho...BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to pathological type.We hypothesize that patients with SRCC and with different SRCC pathological components have different probability of lymph node metastasis(LNM).AIM To establish models to predict LNM in early GC(EGC),including early gastric SRCC.METHODS Clinical data from EGC patients who had undergone gastrectomy at the First Affiliated Hospital of Nanjing Medical University from January 2012 to March 2022 were reviewed.The patients were divided into three groups based on type:Pure SRCC,mixed SRCC,and non-signet ring cell carcinoma(NSRC).The risk factors were identified through statistical tests using SPSS 23.0,R,and EmpowerStats software.RESULTS A total of 1922 subjects with EGC were enrolled in this study,and included 249 SRCC patients and 1673 NSRC patients,while 278 of the patients(14.46%)presented with LNM.Multivariable analysis showed that gender,tumor size,depth of invasion,lymphovascular invasion,ulceration,and histological subtype were independent risk factors for LNM in EGC.Establishment and analysis using prediction models of EGC showed that the artificial neural network model was better than the logistic regression model in terms of sensitivity and accuracy(98.0%vs 58.1%,P=0.034;88.4%vs 86.8%,P<0.001,respectively).Among the 249 SRCC patients,LNM was more common in mixed(35.06%)rather than in pure SRCC(8.42%,P<0.001).The area under the ROC curve of the logistic regression model for LNM in SRCC was 0.760(95%CI:0.682-0.843),while the area under the operating characteristic curve of the internal validation set was 0.734(95%CI:0.643-0.826).The subgroups analysis of pure types showed that LNM was more common in patients with a tumor size>2 cm(OR=5.422,P=0.038).CONCLUSION A validated prediction model was developed to recognize the risk of LNM in EGC and early gastric SRCC,which can aid in pre-surgical decision making of the best method of treatment for patients.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is the most common type of primary liver cancer.With highly invasive biological characteristics and a lack of obvious clinical manifestations,HCC usually has a poor prognosis an...BACKGROUND Hepatocellular carcinoma(HCC)is the most common type of primary liver cancer.With highly invasive biological characteristics and a lack of obvious clinical manifestations,HCC usually has a poor prognosis and ranks fourth in cancer mortality.The aetiology and exact molecular mechanism of primary HCC are still unclear.AIM To select the characteristic genes that are significantly associated with the prognosis of HCC patients and construct a prognosis model of this malignancy.METHODS By comparing the gene expression levels of patients with different cancer grades of HCC,we screened out differentially expressed genes associated with tumour grade.By protein-protein interaction(PPI)network analysis,we obtained the top 2 PPI networks and hub genes from these differentially expressed genes.By using least absolute shrinkage and selection operator Cox regression,13 prognostic genes were selected for feature extraction,and a prognostic risk model of HCC was established.RESULTS The model had significant prognostic ability in HCC.We also analysed the biological functions of these prognostic genes.CONCLUSION By comparing the gene profiles of patients with different stages of HCC,We have constructed a prognosis model consisting of 13 genes that have important prognostic value.This model has good application value and can be explained clinically.展开更多
AIM:To study the effects of Helicobacter pylori(H. pylori)tumor necrosis factor-α(TNF)inducing protein (Tip-α)on cytokine expression and its mechanism. METHODS:We cloned Tip-αfrom the H.pylori strain 26695,transfor...AIM:To study the effects of Helicobacter pylori(H. pylori)tumor necrosis factor-α(TNF)inducing protein (Tip-α)on cytokine expression and its mechanism. METHODS:We cloned Tip-αfrom the H.pylori strain 26695,transformed Escherichia coli with an expression plasmid,and then confirmed the expression product by Western blotting.Using different concentrations of Tip-αthat affected SGC7901 and GES-1 cells at different times,we assessed cytokine levels using enzyme-linked immunosorbent assay.We blocked SGC7901 cells with pyrrolidine dithiocarbamate(PDTC),a specific inhibitor of nuclear factorκB(NF-κB).We then detected interleukin(IL)-1βand TNF-αlevels in SGC7901 cells. RESULTS:Western blot analysis using an anti-Tip-α antibody revealed a 23-kDa protein,which indicated that recombinant Tip-αprotein was recombined successfully.The levels of IL-1β,IL-8 and TNF-αwere sig-nificantly higher following Tip-αinterference,whether GES-1 cells or SGC-7901 cells were used(P<0.05).However,the levels of cytokines(including IL-1β,IL-8 and TNF-α)secreted by SGC-7901 cells were greater than those secreted by GES-1 cells following treatment with Tip-αat the same concentration and for the same duration(P<0.05).After blocking NF-κB with PDTC, the cells(GES-1 cells and SGC-7901 cells)underwent interference with Tip-α.We found that IL-1βand TNF-αlevels were significantly decreased compared to cells that only underwent Tip-αinterference(P<0.05). CONCLUSION:Tip-αplays an important role in cyto-kine expression through NF-κB.展开更多
AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provid...AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provided data dealing with H.pylori infection in patients with migraine,as well as healthy controls,were selected.Meta-analysis was carried out using the odds ratio(OR)with a fixed or random effects model,and a 95%CI for the OR was calculated.An unconditional logistic regression model was used to analyze potential parameters related to H.pylori prevalence.Subgroup analyses were conducted as methods of detection and evidence grade.RESULTS:Five case-control studies published between 2000 and 2013 were finally identified,involving 903 patients,with a total H.pylori infection rateof 39.31%.The prevalence of H.pylori infection was significantly greater in migraineurs than in controls(44.97%vs 33.26%,OR=1.92,95%CI:1.05-3.51,P=0.001).A sensitivity test indicated that no single study dominated the combined results.Univariate regression analysis found that publication year,geographical distribution and evidence grade were relevant to the results and were the main reason for the heterogeneity.Subgroup analysis found a significantly greater infection rate of H.pylori in Asian patients with migraine,but no statistically significant infection rate in European patients.The ORs were 3.48(95%CI:2.09-5.81,P=0.000)and 1.19(95%CI:0.86-1.65,P=0.288),respectively.CONCLUSION:The pooled data suggest a trend of more frequent H.pylori infections in patients with migraine.展开更多
Objective:Study the clinical effect of atorvastatin combined with clopidogrel on patients with progressive cerebral infarction of intracranial aortic stenosis.Methods: Chose eighty-eight patients with progressive cere...Objective:Study the clinical effect of atorvastatin combined with clopidogrel on patients with progressive cerebral infarction of intracranial aortic stenosis.Methods: Chose eighty-eight patients with progressive cerebral infarction in our hospital from June 2014 to October 2015. They were randomly divided into study group and study group, 44 cases in each group. The patients in the control group were treated with oral clopidogrel. On the basis of this, the patients in the study group were treated with atorvastatin;all patients were treated for 4 weeks. Compared the total effective rate, adverse reaction rate, coagulation function index and blood lipid level between the two groups, and compared the NIHSS score and daily life ability (ADL) score between the two groups before and after treatment.Results: The total effective rate (90.90%) was significantly higher in the study group than in the control group (72.73%). After treatment, the NIHSS score of the study group was significantly lower than that of the control group. The levels of LDL-C, TG and TC in the study group were significantly lower than those in the control group, and HDL-C was significantly higher than that in the control group. There was no significant difference between the two groups.Conclusion: Clopidogrel combined with atorvastatin has a significant effect on the patients with intracranial large artery stenosis and improve the neurological function and quality of life. It is safe and reliable. It is worthy to be popularized.展开更多
Artificial synapses and neurons are crucial milestones for neuromorphic computing hardware,and memristors with resistive and threshold switching characteristics are regarded as the most promising candidates for the co...Artificial synapses and neurons are crucial milestones for neuromorphic computing hardware,and memristors with resistive and threshold switching characteristics are regarded as the most promising candidates for the construction of hardware neural networks.However,most of the memristors can only operate in one mode,that is,resistive switching or threshold switching,and distinct memristors are required to construct fully memristive neuromorphic computing hardware,making it more complex for the fabrication and integration of the hardware.Herein,we propose a flexible dual-mode memristor array based on core–shell CsPbBr3@graphdiyne nanocrystals,which features a 100%transition yield,small cycle-to-cycle and device-to-device variability,excellent flexibility,and environmental stability.Based on this dual-mode memristor,homo-material-based fully memristive neuromorphic computing hardware—a power-free artificial nociceptive signal processing system and a spiking neural network—are constructed for the first time.Our dual-mode memristors greatly simplify the fabrication and integration of fully memristive neuromorphic systems.展开更多
To the Editor:Gastric cancer(GC)is one of the most commonly diagnosed malignancies worldwide.[1,2]Recent studies show that an aberrant gastric microbiota contributes to the onset and progression of GC.[3]A distinct cl...To the Editor:Gastric cancer(GC)is one of the most commonly diagnosed malignancies worldwide.[1,2]Recent studies show that an aberrant gastric microbiota contributes to the onset and progression of GC.[3]A distinct cluster of bacteria consisting of Peptostreptococcus,Streptococcus,and Parvimonas among others is associated with gastric atrophy and intestinal metaplasia(IM).[4]However,the gastric microbiome is highly dynamic and influenced by several factors,including diet,xenobiotics,proton pump inhibitors,physiological changes,and host genetics.展开更多
基金Supported by National Natural Science Foundation of China,No.82100594.
文摘BACKGROUND Helicobacter pylori(H.pylori)infection is related to various extragastric diseases including type 2 diabetes mellitus(T2DM).However,the possible mechanisms connecting H.pylori infection and T2DM remain unknown.AIM To explore potential molecular connections between H.pylori infection and T2DM.METHODS We extracted gene expression arrays from three online datasets(GSE60427,GSE27411 and GSE115601).Differentially expressed genes(DEGs)commonly present in patients with H.pylori infection and T2DM were identified.Hub genes were validated using human gastric biopsy samples.Correlations between hub genes and immune cell infiltration,miRNAs,and transcription factors(TFs)were further analyzed.RESULTS A total of 67 DEGs were commonly presented in patients with H.pylori infection and T2DM.Five significantly upregulated hub genes,including TLR4,ITGAM,C5AR1,FCER1G,and FCGR2A,were finally identified,all of which are closely related to immune cell infiltration.The gene-miRNA analysis detected 13 miRNAs with at least two gene cross-links.TF-gene interaction networks showed that TLR4 was coregulated by 26 TFs,the largest number of TFs among the 5 hub genes.CONCLUSION We identified five hub genes that may have molecular connections between H.pylori infection and T2DM.This study provides new insights into the pathogenesis of H.pylori-induced onset of T2DM.
基金National Natural Science Foundation of China,No.82200625 and No.82100595Youth Talent Development Program,No.YNRCQN0313+2 种基金Young Scholar Fostering Fund of the First Affiliated Hospital of Nanjing Medical University,No.PY2021023Top Talent of Changzhou“The 14th Five-Year Plan”High-Level Health Talents Training Project,No.2022CZBJ051Natural Science Foundation of Jiangsu Province,China,No.BK20210958.
文摘BACKGROUND Signet-ring cell carcinoma(SRCC)was previously thought to have a worse prognosis than other differentiated gastric cancer(GC),however,recent studies have shown that the prognosis of SRCC is related to pathological type.We hypothesize that patients with SRCC and with different SRCC pathological components have different probability of lymph node metastasis(LNM).AIM To establish models to predict LNM in early GC(EGC),including early gastric SRCC.METHODS Clinical data from EGC patients who had undergone gastrectomy at the First Affiliated Hospital of Nanjing Medical University from January 2012 to March 2022 were reviewed.The patients were divided into three groups based on type:Pure SRCC,mixed SRCC,and non-signet ring cell carcinoma(NSRC).The risk factors were identified through statistical tests using SPSS 23.0,R,and EmpowerStats software.RESULTS A total of 1922 subjects with EGC were enrolled in this study,and included 249 SRCC patients and 1673 NSRC patients,while 278 of the patients(14.46%)presented with LNM.Multivariable analysis showed that gender,tumor size,depth of invasion,lymphovascular invasion,ulceration,and histological subtype were independent risk factors for LNM in EGC.Establishment and analysis using prediction models of EGC showed that the artificial neural network model was better than the logistic regression model in terms of sensitivity and accuracy(98.0%vs 58.1%,P=0.034;88.4%vs 86.8%,P<0.001,respectively).Among the 249 SRCC patients,LNM was more common in mixed(35.06%)rather than in pure SRCC(8.42%,P<0.001).The area under the ROC curve of the logistic regression model for LNM in SRCC was 0.760(95%CI:0.682-0.843),while the area under the operating characteristic curve of the internal validation set was 0.734(95%CI:0.643-0.826).The subgroups analysis of pure types showed that LNM was more common in patients with a tumor size>2 cm(OR=5.422,P=0.038).CONCLUSION A validated prediction model was developed to recognize the risk of LNM in EGC and early gastric SRCC,which can aid in pre-surgical decision making of the best method of treatment for patients.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is the most common type of primary liver cancer.With highly invasive biological characteristics and a lack of obvious clinical manifestations,HCC usually has a poor prognosis and ranks fourth in cancer mortality.The aetiology and exact molecular mechanism of primary HCC are still unclear.AIM To select the characteristic genes that are significantly associated with the prognosis of HCC patients and construct a prognosis model of this malignancy.METHODS By comparing the gene expression levels of patients with different cancer grades of HCC,we screened out differentially expressed genes associated with tumour grade.By protein-protein interaction(PPI)network analysis,we obtained the top 2 PPI networks and hub genes from these differentially expressed genes.By using least absolute shrinkage and selection operator Cox regression,13 prognostic genes were selected for feature extraction,and a prognostic risk model of HCC was established.RESULTS The model had significant prognostic ability in HCC.We also analysed the biological functions of these prognostic genes.CONCLUSION By comparing the gene profiles of patients with different stages of HCC,We have constructed a prognosis model consisting of 13 genes that have important prognostic value.This model has good application value and can be explained clinically.
文摘AIM:To study the effects of Helicobacter pylori(H. pylori)tumor necrosis factor-α(TNF)inducing protein (Tip-α)on cytokine expression and its mechanism. METHODS:We cloned Tip-αfrom the H.pylori strain 26695,transformed Escherichia coli with an expression plasmid,and then confirmed the expression product by Western blotting.Using different concentrations of Tip-αthat affected SGC7901 and GES-1 cells at different times,we assessed cytokine levels using enzyme-linked immunosorbent assay.We blocked SGC7901 cells with pyrrolidine dithiocarbamate(PDTC),a specific inhibitor of nuclear factorκB(NF-κB).We then detected interleukin(IL)-1βand TNF-αlevels in SGC7901 cells. RESULTS:Western blot analysis using an anti-Tip-α antibody revealed a 23-kDa protein,which indicated that recombinant Tip-αprotein was recombined successfully.The levels of IL-1β,IL-8 and TNF-αwere sig-nificantly higher following Tip-αinterference,whether GES-1 cells or SGC-7901 cells were used(P<0.05).However,the levels of cytokines(including IL-1β,IL-8 and TNF-α)secreted by SGC-7901 cells were greater than those secreted by GES-1 cells following treatment with Tip-αat the same concentration and for the same duration(P<0.05).After blocking NF-κB with PDTC, the cells(GES-1 cells and SGC-7901 cells)underwent interference with Tip-α.We found that IL-1βand TNF-αlevels were significantly decreased compared to cells that only underwent Tip-αinterference(P<0.05). CONCLUSION:Tip-αplays an important role in cyto-kine expression through NF-κB.
基金Supported by National Natural Science Foundation of China,No.81270476the Priority Academic Program Development of Jiangsu Higher Education Institutions,JX10231801
文摘AIM:To quantify the association between Helicobacter pylori(H.pylori)infection and migraine.METHODS:A systematic literature search of PubMed and EMBASE was conducted from inception to December 2013.Studies that provided data dealing with H.pylori infection in patients with migraine,as well as healthy controls,were selected.Meta-analysis was carried out using the odds ratio(OR)with a fixed or random effects model,and a 95%CI for the OR was calculated.An unconditional logistic regression model was used to analyze potential parameters related to H.pylori prevalence.Subgroup analyses were conducted as methods of detection and evidence grade.RESULTS:Five case-control studies published between 2000 and 2013 were finally identified,involving 903 patients,with a total H.pylori infection rateof 39.31%.The prevalence of H.pylori infection was significantly greater in migraineurs than in controls(44.97%vs 33.26%,OR=1.92,95%CI:1.05-3.51,P=0.001).A sensitivity test indicated that no single study dominated the combined results.Univariate regression analysis found that publication year,geographical distribution and evidence grade were relevant to the results and were the main reason for the heterogeneity.Subgroup analysis found a significantly greater infection rate of H.pylori in Asian patients with migraine,but no statistically significant infection rate in European patients.The ORs were 3.48(95%CI:2.09-5.81,P=0.000)and 1.19(95%CI:0.86-1.65,P=0.288),respectively.CONCLUSION:The pooled data suggest a trend of more frequent H.pylori infections in patients with migraine.
文摘Objective:Study the clinical effect of atorvastatin combined with clopidogrel on patients with progressive cerebral infarction of intracranial aortic stenosis.Methods: Chose eighty-eight patients with progressive cerebral infarction in our hospital from June 2014 to October 2015. They were randomly divided into study group and study group, 44 cases in each group. The patients in the control group were treated with oral clopidogrel. On the basis of this, the patients in the study group were treated with atorvastatin;all patients were treated for 4 weeks. Compared the total effective rate, adverse reaction rate, coagulation function index and blood lipid level between the two groups, and compared the NIHSS score and daily life ability (ADL) score between the two groups before and after treatment.Results: The total effective rate (90.90%) was significantly higher in the study group than in the control group (72.73%). After treatment, the NIHSS score of the study group was significantly lower than that of the control group. The levels of LDL-C, TG and TC in the study group were significantly lower than those in the control group, and HDL-C was significantly higher than that in the control group. There was no significant difference between the two groups.Conclusion: Clopidogrel combined with atorvastatin has a significant effect on the patients with intracranial large artery stenosis and improve the neurological function and quality of life. It is safe and reliable. It is worthy to be popularized.
基金Natural Science Foundation of Tianjin City,Grant/Award Number:19JCYBJC17300National Natural Science Foundation of China,Grant/Award Numbers:21790052,51802220。
文摘Artificial synapses and neurons are crucial milestones for neuromorphic computing hardware,and memristors with resistive and threshold switching characteristics are regarded as the most promising candidates for the construction of hardware neural networks.However,most of the memristors can only operate in one mode,that is,resistive switching or threshold switching,and distinct memristors are required to construct fully memristive neuromorphic computing hardware,making it more complex for the fabrication and integration of the hardware.Herein,we propose a flexible dual-mode memristor array based on core–shell CsPbBr3@graphdiyne nanocrystals,which features a 100%transition yield,small cycle-to-cycle and device-to-device variability,excellent flexibility,and environmental stability.Based on this dual-mode memristor,homo-material-based fully memristive neuromorphic computing hardware—a power-free artificial nociceptive signal processing system and a spiking neural network—are constructed for the first time.Our dual-mode memristors greatly simplify the fabrication and integration of fully memristive neuromorphic systems.
文摘To the Editor:Gastric cancer(GC)is one of the most commonly diagnosed malignancies worldwide.[1,2]Recent studies show that an aberrant gastric microbiota contributes to the onset and progression of GC.[3]A distinct cluster of bacteria consisting of Peptostreptococcus,Streptococcus,and Parvimonas among others is associated with gastric atrophy and intestinal metaplasia(IM).[4]However,the gastric microbiome is highly dynamic and influenced by several factors,including diet,xenobiotics,proton pump inhibitors,physiological changes,and host genetics.
