The effects of acupuncture on bone biomechanical properties and histomorphometry in ovariectomized(OVX) rats were studied. Twenty-four 8-week-old female Sprague-Dawley rats.were randomly assigned tothree groups:sham,m...The effects of acupuncture on bone biomechanical properties and histomorphometry in ovariectomized(OVX) rats were studied. Twenty-four 8-week-old female Sprague-Dawley rats.were randomly assigned tothree groups:sham,model and acupuncture. Rats in the model and acupuncture groups were ovariectomized,while those in the sham group underwent a sham operation. All rats were anesthetized and fastened for 15minutes,and for the acupuncture group,needling on Pishu (BL20) and Shenshu (BL23) was performed.BlOod and urine were collected to measure serum osteocalcin (OC) and urinary calcium,phosphorus ordeoxypyridinoline (Dpd). After 16 weeks of treatment,all the rats were killed and their tibiae and femorawere removed. The tibiae were used for analyses of bone histomorphometry and the femora for a three-pointbending test. Acupuncture gave significant protection against ovariectomy-caused decline on femoral strengthin the mechanical test,increased the trabecular bone volume and thickness,lowered the trabecular separationof tibiae and restricted the excretion of phosphorus and Dpd,while promoting the concentrations of serumosteocalcin as compared with model rats. These results seemed to indicate that acupuncture on the points ofPishu (BL20) and Shenshu (BL23) not only promoted the bone formation but also suppressed the boneresorption induced by OVX in osteoporotic rats,which suggests that it would be a potentially useful andconvenient method in preventing osteoporosis.展开更多
文摘The effects of acupuncture on bone biomechanical properties and histomorphometry in ovariectomized(OVX) rats were studied. Twenty-four 8-week-old female Sprague-Dawley rats.were randomly assigned tothree groups:sham,model and acupuncture. Rats in the model and acupuncture groups were ovariectomized,while those in the sham group underwent a sham operation. All rats were anesthetized and fastened for 15minutes,and for the acupuncture group,needling on Pishu (BL20) and Shenshu (BL23) was performed.BlOod and urine were collected to measure serum osteocalcin (OC) and urinary calcium,phosphorus ordeoxypyridinoline (Dpd). After 16 weeks of treatment,all the rats were killed and their tibiae and femorawere removed. The tibiae were used for analyses of bone histomorphometry and the femora for a three-pointbending test. Acupuncture gave significant protection against ovariectomy-caused decline on femoral strengthin the mechanical test,increased the trabecular bone volume and thickness,lowered the trabecular separationof tibiae and restricted the excretion of phosphorus and Dpd,while promoting the concentrations of serumosteocalcin as compared with model rats. These results seemed to indicate that acupuncture on the points ofPishu (BL20) and Shenshu (BL23) not only promoted the bone formation but also suppressed the boneresorption induced by OVX in osteoporotic rats,which suggests that it would be a potentially useful andconvenient method in preventing osteoporosis.
