Fractional anisotropy values in diffusion tensor imaging can quantitatively reflect the consistency of nerve fibers after brain damage, where higher values generally indicate less damage to nerve fibers. Therefore, we...Fractional anisotropy values in diffusion tensor imaging can quantitatively reflect the consistency of nerve fibers after brain damage, where higher values generally indicate less damage to nerve fibers. Therefore, we hypothesized that diffusion tensor imaging could be used to evaluate the effect of mild hypothermia on diffuse axonal injury. A total of 102 patients with diffuse axonal injury were randomly divided into two groups: normothermic and mild hypothermic treatment groups. Patient's modified Rankin scale scores 2 months after mild hypothermia were significantly lower than those for the normothermia group. The difference in average fractional anisotropy value for each region of interest before and after mild hypothermia was 1.32–1.36 times higher than the value in the normothermia group. Quantitative assessment of diffusion tensor imaging indicates that mild hypothermia therapy may be beneficial for patients with diffuse axonal injury.展开更多
The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney’s free falling method.The brains were harvested at 2,6 and 24 hours,and at 3 and 5days after injury.Changes ...The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney’s free falling method.The brains were harvested at 2,6 and 24 hours,and at 3 and 5days after injury.Changes in brain water content were determined using the wet and dry weights.Our results showed that water content of tissue significantly increased after traumatic brain injury,and reached minimum at 24 hours.Hematoxylin-eosin staining revealed pathological impairment of brain tissue at each time point after injury,particularly at 3 days,with nerve cell edema,degeneration,and necrosis observed,and the apoptotic rate significantly increased.Immunohistochemistry and western blot analysis revealed that the expression of occludin at the injured site gradually decreased as injury time advanced and reached a minimum at 3 days after injury;the expression of connexin 43 gradually increased as injury time advanced and reached a peak at 24 hours after injury.The experimental findings indicate that changes in occludin and connexin 43 expression were consistent with the development of brain edema,and may reflect the pathogenesis of brain injury.展开更多
基金supported by the Natural Science Foundation of Guangdong Province in China,No.10151600101000002
文摘Fractional anisotropy values in diffusion tensor imaging can quantitatively reflect the consistency of nerve fibers after brain damage, where higher values generally indicate less damage to nerve fibers. Therefore, we hypothesized that diffusion tensor imaging could be used to evaluate the effect of mild hypothermia on diffuse axonal injury. A total of 102 patients with diffuse axonal injury were randomly divided into two groups: normothermic and mild hypothermic treatment groups. Patient's modified Rankin scale scores 2 months after mild hypothermia were significantly lower than those for the normothermia group. The difference in average fractional anisotropy value for each region of interest before and after mild hypothermia was 1.32–1.36 times higher than the value in the normothermia group. Quantitative assessment of diffusion tensor imaging indicates that mild hypothermia therapy may be beneficial for patients with diffuse axonal injury.
基金supported by the Natural Science Foundation of Guangdong Province,No.10151600101000002
文摘The experimental model of traumatic brain injury was established in Sprague-Dawley rats according to Feeney’s free falling method.The brains were harvested at 2,6 and 24 hours,and at 3 and 5days after injury.Changes in brain water content were determined using the wet and dry weights.Our results showed that water content of tissue significantly increased after traumatic brain injury,and reached minimum at 24 hours.Hematoxylin-eosin staining revealed pathological impairment of brain tissue at each time point after injury,particularly at 3 days,with nerve cell edema,degeneration,and necrosis observed,and the apoptotic rate significantly increased.Immunohistochemistry and western blot analysis revealed that the expression of occludin at the injured site gradually decreased as injury time advanced and reached a minimum at 3 days after injury;the expression of connexin 43 gradually increased as injury time advanced and reached a peak at 24 hours after injury.The experimental findings indicate that changes in occludin and connexin 43 expression were consistent with the development of brain edema,and may reflect the pathogenesis of brain injury.
