The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remain...The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remains unclear.Retrovirnl vector pSEB61 was retroftted in established HCT116 siKCN and SW480-siKCN cells to silence KCNQ1 OT1.Cellular proliferation was measured using CCK8 assay,and flow cytometry(FCM)detected cell cydle changes.RNA sequencing(RNA Seq)analysis showed differentially expressed genes(DEGs).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.RT-qPCR,immunofluorescence,and western blotting were carried out to validate downstream gene expressions.The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts.Our data revealed that the silencing of KONQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase.RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cyde.RT qPCR,immunofluorescence,and western blotting confirmed downstream CCNE2 and PCNA gene expressions.HCT116 siKCN cells signifcantly suppressed tumorigenesis in BALB/c nude mice.Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer.展开更多
Liangjia Village earth fissure,one of the 79 earth fissures along the Kouzhen-Guanshan fault located in the northern Weihe Basin,causes severe damage to buildings and farmlands.Since the late 1950s,40 earth fissures h...Liangjia Village earth fissure,one of the 79 earth fissures along the Kouzhen-Guanshan fault located in the northern Weihe Basin,causes severe damage to buildings and farmlands.Since the late 1950s,40 earth fissures have occurred in a similar east-west(EW)direction parallel to the Kouzhen-Guanshan fault,and a further 39 earth fissures have occurred with north-west,east-north,or north-east orientations intersecting the fault.In this study,a case study of Liangjia Village earth fissure was conducted to investigate the mechanisms of fissure generation in detail.Geotechnical and geophysical methods including measurements,trenching,drilling,and seismic exploration were used to reveal the basic characteristics,geological background,and origin model of the Liangjia Village earth fissure.This earth fissure,with a total length of 800 m in 2014,runs EW parallel to the Kouzhen-Guanshan fault,and it has damaged buildings and farmlands by forming sinkholes,gullies,subsidence,and a scarp.The trenching results indicated that this fissure underwent multi-phase activity.Analysis of geological drilling and shallow seismic profiling results showed that the fissure also possessed characteristics of a synsedimentary fault.Regarding its genesis,the fissure was formed through the combined actions of three factors:an earthquake created the fissure,the Kouzhen-Guanshan fault controlled its development,and loess erosion and groundwater runoff subsequently enlarged the fissure.Regional extension first generated many buried faults along the hanging wall of the Kouzhen-Guanshan fault before seismic activity caused the buried faults to propagate to the surface,where loess erosion and ground-water runoff promoted the formation of the current earth fissure.展开更多
基金the Scientific Research Project of Anhui Provincial Health Commission in 2021(#AHWJ2021b109 to LS)Scientific and Technological Research Program of Chongqing Municipal Education Commission(#KJZD-K201900402 to TZ)+1 种基金Special Fund for Wannan Medical College Scholar Project(#WK2021F07)Educational Commission of Anhui Province of China(2022AH051241).
文摘The role of lncRNA KCNQ1 opposite strand/antisense transcript 1(KCNQ1OT1)in colon cancer involves various tumorigenic processes and has been studed widely.However,the mechanism by which it promotes colon cancer remains unclear.Retrovirnl vector pSEB61 was retroftted in established HCT116 siKCN and SW480-siKCN cells to silence KCNQ1 OT1.Cellular proliferation was measured using CCK8 assay,and flow cytometry(FCM)detected cell cydle changes.RNA sequencing(RNA Seq)analysis showed differentially expressed genes(DEGs).Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways.RT-qPCR,immunofluorescence,and western blotting were carried out to validate downstream gene expressions.The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts.Our data revealed that the silencing of KONQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase.RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cyde.RT qPCR,immunofluorescence,and western blotting confirmed downstream CCNE2 and PCNA gene expressions.HCT116 siKCN cells signifcantly suppressed tumorigenesis in BALB/c nude mice.Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer.
基金This study was funded by the National Basic Research Program of China(No.2014CB744700)the High Speed Railway Joint Fund of NSFC(No.U1934208)+4 种基金the Key R&D Program of Jiangxi Province,China(No.20202BBGL73079)the CMEC Technology Incubation Project(CMEC-KJFH-2018-02)the National Natural Science Foundation(Grant No.41702298)Supported by the Fundamental Research Funds for the Central Universities,CHD(Nos.300102260402 and 300102269203)the National Geological Survey of China(Nos.121201001000150122 and DD20160235).
文摘Liangjia Village earth fissure,one of the 79 earth fissures along the Kouzhen-Guanshan fault located in the northern Weihe Basin,causes severe damage to buildings and farmlands.Since the late 1950s,40 earth fissures have occurred in a similar east-west(EW)direction parallel to the Kouzhen-Guanshan fault,and a further 39 earth fissures have occurred with north-west,east-north,or north-east orientations intersecting the fault.In this study,a case study of Liangjia Village earth fissure was conducted to investigate the mechanisms of fissure generation in detail.Geotechnical and geophysical methods including measurements,trenching,drilling,and seismic exploration were used to reveal the basic characteristics,geological background,and origin model of the Liangjia Village earth fissure.This earth fissure,with a total length of 800 m in 2014,runs EW parallel to the Kouzhen-Guanshan fault,and it has damaged buildings and farmlands by forming sinkholes,gullies,subsidence,and a scarp.The trenching results indicated that this fissure underwent multi-phase activity.Analysis of geological drilling and shallow seismic profiling results showed that the fissure also possessed characteristics of a synsedimentary fault.Regarding its genesis,the fissure was formed through the combined actions of three factors:an earthquake created the fissure,the Kouzhen-Guanshan fault controlled its development,and loess erosion and groundwater runoff subsequently enlarged the fissure.Regional extension first generated many buried faults along the hanging wall of the Kouzhen-Guanshan fault before seismic activity caused the buried faults to propagate to the surface,where loess erosion and ground-water runoff promoted the formation of the current earth fissure.
